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Définition d’un indice composition pour le phytoplancton en Manche- Atlantique, à partir des données du micro-phytoplancton du REPHY et des réseaux régionaux, et des mesures complémentaires de la flore phytoplanctonique acquises avec des techniques novatrices. Action 3 – Livrable 1. Propositions pour un indice de composition du phytoplancton, basé sur les résultats des méthodes microscopie, pigments et diversité génétique. Rapport final, février 2016 ArchiMer
Hernandez Farinas, Tania; Brun, Melanie; Siano, Raffaele; Delmas, Daniel.
This deliverable is composed of two reports which propose indice of phytoplankton composition, based respectively on the results of pigment and genetic diversity methods, and the results of microscopy method. Proposals based on the results of pigment and genetic diversity methods. The present work is a continuation of several studies carried out in the past (Siano and Delmas, 2013, 2015) on investigating an indicator of phytoplankton diversity using two methods complementary to optical microscopy: the genetic diversity analyzed with metabarcoding (exhaustive analysis of the diversity and relative abundance of OTUs, i.e. groups of sequences identified by their genetic resemblance) and the pigmentary diversity (analysis of the diversity and abundance of...
Tipo: Text Palavras-chave: Metabarcoding; OTUs; Pigments; Picophytoplancton; Indice composition; Niche ecologique; Mamiellophyceae; Chlorophylle b; Rade de Brest; Bretagne sud; Panache de la Gironde; Denombrements du micro-phytoplancton; Abondance totale; Monotonicite; Linearite; Pressions; Facade Manche-Atlantique; Metabarcoding; OTUs; Pigments; Picophytoplankton; Composition indice; Ecological niche; Mamiellophyceae; Chlorophyll b; Brest Bay; Southern Britain; Gironde plume; Micro-phytoplankton counts; Total abundance; Monotonicity; Linearity; Pressure; Channel-Atlantic coast.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00363/47421/47426.pdf
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Movement patterns of humpback whales (Megaptera novaeangliae) reoccupying a Brazilian breeding ground Biota Neotropica
Gonçalves,Maria Isabel Carvalho; Sousa-Lima,Renata Santoro de; Teixeira,Niel Nascimento; Carvalho,Gustavo Henrique; Danilewicz,Daniel; Baumgarten,Júlio Ernesto.
Abstract: The population of humpback whales from breeding stock A is increasing, and little is known about the routes used by humpbacks that move north of the main calving area of Brazil, the Abrolhos Bank. The aim of this study was to describe the movements of humpback whales in a reoccupation wintering area (Serra Grande, Bahia state, Brazil) based on land-based surveys to test if movement patterns change during the season and between years, due to group composition, behavioral state, and distance to the coast. The mean leg speed of the groups sighted was 6.88 (±2.92) km/h, and leg speed was positively correlated with distance to the coast. There was an increase in leg speed and distance to the coast with increasing number of escorts in the groups with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Calving area; Distance to the coast; Land-based station; Linearity; Speed.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032018000400210
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Use of real-time PCR to evaluate two DNA extraction methods from food Ciênc. Tecnol. Aliment.
Branquinho,Maria Regina; Ferreira,Renata Trotta Barroso; Cardarelli-Leite,Paola.
The DNA extraction is a critical step in Genetically Modified Organisms analysis based on real-time PCR. In this study, the CTAB and DNeasy methods provided good quality and quantity of DNA from the texturized soy protein, infant formula, and soy milk samples. Concerning the Certified Reference Material consisting of 5% Roundup Ready® soybean, neither method yielded DNA of good quality. However, the dilution test applied in the CTAB extracts showed no interference of inhibitory substances. The PCR efficiencies of lectin target amplification were not statistically different, and the coefficients of correlation (R²) demonstrated high degree of correlation between the copy numbers and the threshold cycle (Ct) values. ANOVA showed suitable adjustment of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA extraction; Genetically modified organisms; GMO; Food analysis; Real-time PCR; Linearity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100017
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Use of the correlation coefficient in agricultural sciences: problems, pitfalls and how to deal with them Anais da ABC (AABC)
Kozak,Marcin; Krzanowski,Wojtek; Tartanus,MaŁgorzata.
This paper discusses a number of aspects concerning the analysis, interpretation and reporting of correlations in agricultural sciences. Various problems that one might encounter with these aspects are identified, and suggestions of how to overcome these problems are proposed. Some of the examples presented show how mistaken and even misleading the interpretation of correlation can be when one ignores simple rules of analysis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Experimental design; Linearity; Path analysis; Simpson's paradox; Statistical analysis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400029
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