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| Registros recuperados: 17 | |
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| Odisi,Estácio Jussie; Silvestrin,Marcela Bruschi; Takahashi,Rodrigo Yoji Uwamori; da Silva,Marcus Adonai Castro; Lima,André Oliveira de Souza. |
| Background: Cellulases and lipases have broad industrial application, which calls for an urgent exploration of microorganisms from extreme environments as valuable source of commercial enzyme. In this context, the present work describes the bioprospection and identification of deep-sea bacteria that produce cellulases and lipases, as well their optimal temperature of activity. Results: The first step of this study was the screening of cellulolytic and lipolytic deep-sea bacteria from sediment and water column, which was conducted with substrates linked with 4-Methylumbelliferyl. Among the 161 strains evaluated, 40 were cellulolytic, 23 were lipolytic and 5 exhibited both activities. Cellulolytic and lipolytic bacteria are more common in sediment than at... |
| Tipo: Journal article |
Palavras-chave: Bacteria; Cellulases; Deep-sea; Lipases. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500018 |
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| Silva,Jane E. S.; Jesus,Paulo C.. |
| In the present work, the ester synthesis in organic media catalyzed by lipases immobilized on chrysotile was studied. Lipases of different sources (Mucor javanicus, Pseudomonas cepacia, Rhizopus oryzae, Aspergillus niger and Candida rugosa) were immobilized on chrysotile, an inexpensive magnesium silicate, and used for esterification of hexanoic, octanoic and lauric acid with methanol, ethanol, 1-butanol and 1-octanol at 25ºC in hexane as solvent. The best results were obtained with Mucor javanicus lipase and lauric acid giving yields of 62-97% of ester. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chrysotile; Lipases; Esters. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652003000200003 |
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| Bevilaqua,Maycon R.R.; Santana Filho,Arquimedes P.; Mangolin,Claudete A.; Oliveira,Arildo J.B.; Machado,Maria De Fátima P.S.. |
| The purpose of this study was to evaluate the chemical, physiological and genetic differences in seeds of cactus of the Cereus genus (mandacaru) cultivated in the Northeast (Picos, State of Piauí) and Southern (Maringá, State of Paraná) regions of Brazil. Over a period of eight days, temperatures of 25°C and 30°C were equally efficient for the germination of all the seeds. Oleic acid (C18:1) was the most common fatty acid found in the seeds collected in the Southern (41%) and Northeast (45.5%) regions. The analysis of lipases indicated that seeds from Maringá have high mean observed and expected heterozygosities and that seeds from Picos have a higher number of alleles per loci. Therefore, the seeds of mandacaru from the semiarid region of Northeast as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cactus; Cereus peruvianus; Cereus jamacaru; Seed germination; Fatty acids; Lipases; Genetic diversity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200765 |
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| Menoncin,Silvana; Domingues,Natalia Molossi; Freire,Denise Maria Guimarães; Oliveira,José Vladimir; Di Luccio,Marco; Treichel,Helen; Oliveira,Débora de. |
| O principal interesse em imobilizar uma enzima é obter um biocatalisador com atividade e estabilidade que não sejam afetadas durante o processo, em comparação à sua forma livre. Aliado ao potencial biotecnológico que as lipases apresentam, a aplicação destas em nível industrial requer a investigação de técnicas viáveis para reutilização e aumento da estabilidade, conferindo relevância aos processos de imobilização. Neste trabalho investigou-se a imobilização da lipase produzida por fermentação em estado sólido utilizando Penicillium verrucosum em dois suportes hidrofóbicos; Accurel EP 1000 e Carvão Ativo. Para a imobilização das lipases foi adicionado 1 g de suporte a 50 mL de uma solução enzimática, estes permaneceram em contato por 2 horas em banho de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipases; Penicillium verrucosum; Imobilização. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612009000200033 |
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| Benjamin,Sailas; Pandey,Ashok. |
| Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40ºC and pH 7-8.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500002 |
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| Benjamin,Sailas; Pandey,Ashok. |
| Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40°C and pH 7-8.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida rugosa; Lipases; Solid cultures; Purification; Characterization. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200016 |
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| Hermes,Eliane; Rocha,Dayane C. da; Orssatto,Fábio; Lucas,Juliana F.R.; Gomes,Simone D.; Sene,Luciane. |
| The aim of this study was to isolate microorganisms that produce lipase and to assess the efficiency of COD removal intreatment of cheese whey under different operating conditions. The microorganisms were isolated from cheese whey and a commercial product; it was selectedthreemicroorganisms that obtained the best response to the lipolytic activity test through the enzyme index. Then, the microorganisms were inoculated in sterilized cheese whey samples, for two pH values (6.2 and 7.0), incubated at 35 °C and 150 rpm in shaker and the lipolityc activity and the efficiency of COD removal were measured in two time periods (24 and 48h). After incubation, it was observed that the treatments showed a good removal efficiency of COD for the pre-treatment and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Waste treatment; Dairy industry; Lipases. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162013000200016 |
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| Lima,Laisy Garcia Ribeiro; Gonçalves,Márcia Monteiro Machado; Couri,Sonia; Melo,Verônica Ferreira; Sant’Ana,Gizele Cardoso Fontes; Costa,Antônio Carlos Augusto da. |
| Abstract This study aimed to evaluate the effects of variables on the process of lipases production by Aspergillus niger C by submerged fermentation (SmF). The production assays were performed in shake flasks for 72 hours at 150 rpm and 32°C. First, a fractional factorial design 25-1 (FFD) was carried out to evaluate the effect of the following process variables: sucrose, ammonium sulphate, soybean oil, yeast extract concentration and pH. After the selection of the variables that significantly influenced the lipase production, a central composite rotational design 22 (CCRD) was used, aiming to find the most favorable operational conditions. The selected assay condition (15.0 g.L-1 sucrose, 4.0 g.L-1 ammonium sulphate, 4.0 g.L-1 soybean oil and 1.0 g.L-1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipases; Submerged fermentation; Aspergillus nige; Experimental design.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100214 |
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| Pinheiro,Thaís da Luz Fontoura; Menoncin,Silvana; Domingues,Natália Molossi; Oliveira,Débora de; Treichel,Helen; Di Luccio,Marco; Freire,Denise Maria Guimarães. |
| The growing interest in lipase production is related to the potential biotechnological applications that these enzymes present. Current studies on lipase production by submerged fermentation involve the use of agro-industrial residues aiming at increasing economic attractiveness. Based on these aspects, the objective of this work was to investigate lipase production by Penicillium verrucosum in submerged fermentation using a conventional medium based on peptone, yeast extract, NaCl and olive oil, and an industrial medium based on corn steep liquor, Prodex Lac (yeast hydrolysate), NaCl and olive oil, as well as to characterize the crude enzymatic extracts obtained. Kinetics of lipase production was evaluated and the highest enzymatic activities, of 3.15 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipases; Penicillium verrucosum; Submerged fermentation; Partial characterization. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612008000200028 |
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| Ferreira-Dias,Suzana; Sandoval,Georgina; Plou,Francisco; Valero,Francisco. |
| The potential of lipases (E.C.3.1.1.3.) as biocatalysts for the production of fatty acid derivatives for the food and nutraceutical industries, such as flavouring esters, fatty acid esters of antioxidants and structured lipids, is enormous, mainly due to their high regio- and stereo-selectivities, in addition to the other well-known advantages of enzymatic processes. The replacement of chemical catalysts by lipases presents great benefits in terms of the nutritional properties of the obtained products and environmental care. The reactions performed for the production of these compounds, as well as the best operation conditions, the biocatalysts used, reactor types and operation mode, are addressed in this review. |
| Tipo: Journal article |
Palavras-chave: Fatty acids derivatives; Lipases; Nutraceutical industries. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300012 |
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| Dalla Rosa,Clarissa; Oliveira,Débora de; Oliveira,José Vladimir. |
| This research note addresses the role of organic solvent amount in the production of fatty acid ethyl esters from soybean oil. N-hexane was chosen as solvent and two commercial immobilized lipases as catalysts, Novozym 435 and Lipozyme IM. The reactions were conducted in 6 hours, varying the solvent to oil ratio from zero to 50 (v/wt) and adopting adopting for Novozym 435: 65 ºC, enzyme concentration (E, wt%) = 5, oil to ethanol molar ratio (R) = 1:10, water addition (H, wt%) = 0, and for Lipozyme IM: 35 ºC, E = 5 wt%, R = 1:3, H = 10 wt%. For Lipozyme IM, an increase in solvent amount is shown to lead to an enhancement of reaction conversion, while a negligible effect was found for Novozym 435. When using 30 mL of solvent the reaction conversions were 88%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biodiesel; Lipases; Organic solvent; Soybean oil. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000100011 |
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| Registros recuperados: 17 | |
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