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Accurate identification of atypical Staphylococcus chromogenes plasma-clotting strains causing bovine mastitis 65
Avellar-Costa,Pedro; Santos,Danielle Cabral dos; Lange,Carla Christine; Laport,Marinella Silva; Brito,Maria Aparecida Vasconcelos Paiva; Rossi,Ciro César; Giambiagi-deMarval,Marcia.
ABSTRACT: We compared the potential of routine techniques used for the identification of Staphylococcus species, aiming to evaluate their accuracy in the detection of 43 Staphylococcus chromogenes strains isolated from bovine mastitis that, despite being a coagulase-negative species, are able to clot plasma. These strains could be mistakenly suspected to be S. aureus and lead to an unappropriated treatment of the disease. MALDI-TOF, PCR-RFLP of the chaperonine gene groEL, and sequencing of the 16S rRNA and elongation factor Tu gene tuf were employed. Results from the four methods were coincident for only half of the strains because of the low accuracy of the groEL PCR-RFLP (51.2% accuracy). Even though all the sequencing results were identical, the high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus chromogenes; Mastitis; MALDI-TOF; Tuf; GroEL.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000600452
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Biochemical and pharmacological study of venom of the wolf spider Lycosa singoriensis 80
Liu,ZH; Qian,W; Li,J; Zhang,Y; Liang,S.
The wolf spider Lycosa singoriensis is a large and venomous spider distributed throughout northwestern China. Like other spider venoms, the wolf spider venom is a chemical cocktail. Its protein content is 0.659 mg protein/mg crude venom as determined by the Lowry method. MALDI-TOF analysis revealed that the venom peptides are highly diverse and may be divided into three groups characterized by three independent molecular ranges: 2,000 to 2,500 Da, 4,800 to 5,500 Da and 7,000 to 8,000 Da, respectively. This molecular distribution differs substantially from those of most spider venoms studied so far. This wolf spider venom has low neurotoxic action on mice, but it can induce hemolysis of human erythrocytes. Furthermore, the venom shows antimicrobial activity...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spider; Lycosa singoriensis; Crude venom; MALDI-TOF; Antimicrobial activity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100008
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Biochemical survey of the polar head of plant glycosylinositolphosphoceramides unravels broad diversity 5
Cacas, Jean-luc; Bure, Corinne; Furt, Fabienne; Maalouf, Jean-paul; Badoc, Alain; Cluzet, Stéphanie; Schmitter, Jean-marie; Antajan, Elvire; Mongrand, Sébastien.
Although Glycosyl-Inositol-Phospho-Ceramides (GIPCs) are the main sphingolipids of plant tissues, they remain poorly characterized in term of structures. This lack of information, notably with regard to polar heads, currently hampers the understanding of GIPC functions in biological systems. This situation prompted us to undertake a large scale-analysis of plant GIPCs: 23 plant species chosen in various phylogenetic groups were surveyed for their total GIPC content. GIPCs were extracted and their polar heads were characterized by negative ion MALDI and ESI mass spectrometry. Our data shed light on an unexpected broad diversity of GIPC distributions within Plantae, and the occurrence of yet-unreported GIPC structures in green and red algae. In monocots,...
Tipo: Text Palavras-chave: Glycosphingolipid; Glycosyl-Inositol-Phospho-Ceramide; GIPC; Mass spectrometry; MALDI-TOF; Polar head; Roots; Photosynthetic tissues; Berries.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00166/27689/25884.pdf
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Direct identification from positive blood broth culture by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) 55
Barberino,Maria Goreth; Silva,Marcio de Oliveira; Arraes,Ana Carolina Palmeiras; Correia,Luís Cláudio; Mendes,Ana Verena.
ABSTRACT Bloodstream infections (BSIs) are among the most concerning bacterial infections. They are one of the leading causes of morbidity and mortality, and occur in 30-70% of critical care patients. The prompt identification of the causative microorganism can help choosing the appropriate antimicrobial therapy that will lead to better clinical outcomes. Blood culture is one of the most relevant tests for microbiological diagnosis of bacterial infections. The introduction of the MALDI-TOF microbiological diagnosis significantly decreased the time of identifying microorganisms. However, it depends on the growth on solid culture medium. In this study, 538 bottles of positive blood cultures were evaluated to test the accuracy of an in house modified...
Tipo: Info:eu-repo/semantics/other Palavras-chave: MALDI-TOF; Blood culture; Bacterial identification.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300339
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Fermentation process for production of apple-based kefir vinegar: microbiological, chemical and sensory analysis 58
Viana,Roberta Oliveira; Magalhães-Guedes,Karina Teixeira; Braga Jr.,Roberto Alves; Dias,Disney Ribeiro; Schwan,Rosane Freitas.
Abstract The aim of this study was to develop a kefir apple-based vinegar and evaluate this fermentation process using new methodology with Biospeckle Laser. Brazilian kefir grains were inoculated in apple must for vinegar production. In this study, the microbial community present in kefir, and correspondent vinegar, was investigated using Matrix Assisted Laser Desorption/Ionization - Time of Flight Mass Spectrometry (MALDI-TOF MS) technique. Saccharomyces cerevisiae, Lactobacillus paracasei, Lactobacillus plantarum, Acetobacter pasteurianus and Acetobacter syzygii were the microbial species identified. S. cerevisiae, L. plantarum, A. pasteurianus and A. syzygii were found in smaller quantities at the beginning of the alcoholic fermentation, but were found...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Saccharomyces; Lactobacillus; Apple; MALDI-TOF; Biospeckle Laser; Vinegar.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300592
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Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry 58
Karcher,Daniel; Grenfell,Rafaella C.; Moreno,Andrea Micke; Moreno,Luisa Zanolli; Vasconcellos,Silvio Arruda; Heinemann,Marcos B.; Almeida Junior,Joao N. de; Juliano,Luiz; Juliano,Maria A..
ABSTRACT Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e.g., Leptospira interrogans and Leptospira kirschneri) may not be discriminated using this technology. Herein, we report an in-house Leptospira reference spectra database using Leptospira reference strains that were validated with a collection...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira; Brazil; Identification; Mass spectrometry; MALDI-TOF.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400900
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Imagerie par spectrométrie de masse de l’accumulation d’acide okadaïque dans la glande digestive de la moule 5
Sam Lone, Anais.
Among the applications that facilitate the study of functional processes in living systems, measurement of the spatial distribution of molecules in biological tissues is currently available. Thus, in the field of human health, this application which targets the molecular distributions of altered tissues can be used, for example, in the diagnosis of diseases such as cancers, Alzheimer's disease, muscular myopathy, but also to recognize the distribution of drugs in animal tissues. The principle of imaging by mass spectrometry is to produce a mapping of the surface of a section of tissue, recording at each point a mass spectrum. All these spectra reconstitute so many "ion density maps", called images, that there are peaks of biological interest in the...
Tipo: Text Palavras-chave: Imagerie par spectrométrie de masse; MALDI-TOF; TOF-SIMS; Acide okadaïque; Glande digestive; Mytilus edulis; Imaging by mass spectrometry; MALDI-TOF; TOF-SIMS; Okadaic acid; Digestive Gland; Mytilus edulis.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00360/47167/47190.pdf
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Influence of microbiome species in hard-to-heal wounds on disease severity and treatment duration 55
Chudobova,Dagmar; Cihalova,Kristyna; Guran,Roman; Dostalova,Simona; Smerkova,Kristyna; Vesely,Radek; Gumulec,Jaromir; Masarik,Michal; Heger,Zbynek; Adam,Vojtech; Kizek,Rene.
ABSTRACT BACKGROUND: Infections, mostly those associated with colonization of wound by different pathogenic microorganisms, are one of the most serious health complications during a medical treatment. Therefore, this study is focused on the isolation, characterization, and identification of microorganisms prevalent in superficial wounds of patients (n = 50) presenting with bacterial infection. METHODS: After successful cultivation, bacteria were processed and analyzed. Initially the identification of the strains was performed through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on comparison of protein profiles (2-30 kDa) with database. Subsequently, bacterial strains from infected wounds were identified by both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial strains; MALDI-TOF; Sequencing; Superficial wounds.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600604
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Process optimization for production and purification of a thermostable, organic solvent tolerant lipase from Acinetobacter sp. AU07 58
Gururaj,P.; Ramalingam,Subramanian; Devi,Ganesan Nandhini; Gautam,Pennathur.
ABSTRACT The purpose of this study was to isolate, purify and optimize the production conditions of an organic solvent tolerant and thermostable lipase from Acinetobacter sp. AU07 isolated from distillery waste. The lipase production was optimized by response surface methodology, and a maximum production of 14.5 U/mL was observed at 30 ºC and pH 7, using a 0.5% (v/v) inoculum, 2% (v/v) castor oil (inducer), and agitation 150 rpm. The optimized conditions from the shake flask experiments were validated in a 3 L lab scale bioreactor, and the lipase production increased to 48 U/mL. The enzyme was purified by ammonium sulfate precipitation and ion exchange chromatography and the overall yield was 36%. SDS-PAGE indicated a molecular weight of 45 kDa for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acinetobacter sp.; Response surface methodology; MALDI-TOF; Organic solvent tolerant lipase; Thermostable lipase.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300647
Registros recuperados: 9
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