|
|
|
|
|
| Klimaszewska, K.; Noceda, C.; Pelletier, G.; Label, P.; Rodriguez, R.; Lelu-Walter, M.A.. |
| Somatic embryogenesis (SE) in conifers is at the base of nearly allbiotechnology products that potentially might be developedincluding transgenic trees. SE is a powerful tool for clonalpropagation that has opened avenues for deployment of superiorclonally replicated planting stock in forest plantations. Althoughthe SE process can be managed for high efficiency there are stillseveral problems that require better understanding of underlyingcauses. One phenomenon, which occurs after the long-termculture of embryogenic tissue, particularly of pines, is the loss ofability to produce mature somatic embryos and consequentlyplants. Such unproductive cultures must be replaced, which is bothexpensive and undesirable. In an attempt to get an insight into... |
| Tipo: Meeting Paper |
Palavras-chave: MASSE EMBRYONNAIRE; EMBRYON SOMATIQUE; METHYLATION DE l'ADN EMBRYONAL MASS; SOMATIC EMBRYO; DNA METHYLATION; MARITIME PINE. |
| Ano: 2006 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008b2b6654e&uri=/notices/prodinra1/2008/11/ |
| |
|
|
| Trontin, J.F.; Reymond, I.; Quoniou, S.; Canlet, F.; Debille, S.; Bruneau, G.; Harvengt, L.; Lelu-Walter, M.A.; Teyssier, C.; Le Mette, C.; Vallance, M.; Label, P.. |
| Pinus pinaster is the main industrial conifer in South-western Europe, especially France. Advanced forestry and conventional genetic breeding for 50 years resulted in a 3-fold increase in wood productivity. Up to 15% increase in growth and straightness were effective with first-generation varieties but genetic redundancy is expected to lower forthcoming genetic gain. Maximizing genetic gain for historical and new selection criteria remains challenging owing to long generation time of Maritime pine and low ability for conventional clonal propagation. Somatic embryogenesis (SE) was considered early in the eighties as a very promising way to fulfil this requirement by capturing the best genetic stock. Evidence for SE in maritime pine was initially reported in... |
| Tipo: Conference Paper |
Palavras-chave: EMBRYOGENESE SOMATIQUE; MATURATION DE L'EMBRYON SOMATIQUE SOMATIC EMBRYOGENESIS; MARITIME PINE; SOMATIC EMBRYO MATURATION. |
| Ano: 2010 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011dc90f870&uri=/notices/prodinra1/2011/05/ |
| |
|
|
| Klimaszewska, K.; Noceda, C.; Pelletier, G.; Label, P.; Rodriguez, R.; Lelu-Walter, M.A.. |
| Pinus pinaster (Ait.) somatic embryogenesis (SE) has been developed during the last decade, and its application in tree improvement programs is underway. Nevertheless, a few more or less important problems still exist, which have an impact on the efficiency of specific SE stages. One phenomenon, which had been observed in embryogenic tissue (embryonal mass, EM) initiated from immature seed, has been the loss of the ability to produce mature somatic embryos after the tissue had been cultured for several months. In an attempt to get insight into the differences between young cultures of EM (3-mo-old since the first subculture) of P. pinaster that produced mature somatic embryos and the same lines of significantly increased age (18-mo-old, aged EM) that... |
| Tipo: Journal Article |
Palavras-chave: EMBRYOGENESE SOMATIQUE; MATURATION DE L'EMBRYON SOMATIQUE; CHANGEMENT BIOCHIMIQUE; METHYLATION DE L'ADN; HORMONE ENDOGENE SOMATIC EMBRYOGENESIS; SOMATIC EMBRYO MATURATION; BIOCHEMICAL CHANGE; DNA METHYLATION; ENDOGENOUS HORMONE; MARITIME PINE. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008454303df&uri=/notices/prodinra1/2009/03/ |
| |
|
|
| Fernandes, L.; Rocheta, M.; Cordeiro, J.; Pereira, S.; Gerber, S.; Oliveira, M.M.; Ribeiro, M.M.. |
| L'apparentement entre parents, la variation de fertilité entre clones et la pollution pollinique font dévier le gain génétique réalisé et la diversité génétique des valeurs attendues en verger à graines de clones à pollinisation libre, en particulier pour une espèce à pollinisation anémophile comme Pinus pinaster Aiton.Ce travail étudie la variation génétique, le système de reproduction et la contamination du pollen d'un verger à graines de clones (VGC) de P. pinaster, en génotypant les 60 clones du VGC et les graines récoltées sur 21 arbres-mères pour trois marqueurs microsatellites nucléaires.La diversité attendue s'est révélée similaire mais l'hétérozygotie observée a diminué de 20 % chez les descendants par rapport aux parents. Le taux... |
| Tipo: Journal Article |
Palavras-chave: VARIATION GENETIQUE; SYSTEME DE REPRODUCTION; FLUX DE GENES; MICROSATELLITES; PIN MARITIME; VERGER A GRAINES DE CLONES; MARQUEUR GENETIC VARIATION; MATING SYSTEM; GENE FLOW; SIMPLE SEQUENCE REPEATS; MARITIME PINE. |
| Ano: 2008 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200892079bd0&uri=/notices/prodinra1/2009/11/ |
| |
|
| |
|
|
| Trontin, J.F.; Reymond, I.; Quoniou, S.; Canlet, F.; Debille, S.; Bruneau, G.; Durandeau, K.; Harvengt, L.; Le Mette, C.; Vallance, M.; Teyssier, C.; Label, P.; Lelu-Walter, M.A.. |
| Maritime pine contributes for a leading part in socio-economic development of the French Aquitania region. Sudden and drastic changes in market value and environmental constraints in recent years would need a significant paradigm shift in current breeding technology to deliver suitable varieties. Field comparison of vegetative propagules is a key towards individual selection and efficient capture of thebest genetic stocks. Clonal propagation is also required for scaling up production of improved varieties.Somatic embryogenesis is considered as the key technology to fulfil such requirements in maritime pine. Significant progress has been made during the last 2 decades using immature zygotic embryos as explants. High genotype capture within family at the... |
| Tipo: Conference Paper |
Palavras-chave: EMBRYOGENESE SOMATIQUE SOMATIC EMBRYOGENESIS; MARITIME PINE. |
| Ano: 2011 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011e84b4143&uri=/notices/prodinra1/2011/05/ |
| |
|
| |
|
|
|
