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	Provedor de dados Inra (20) Autor Estoup, A. (4) Loiseau, A. (4) Chapuis, M.P. (2) Dalleau-Clouet, C. (2) Gauthier, N. (2) Lecoq, M. (2) Lepais, O. (2) Martin, J.F. (2) Michalakis, Y. (2) Abdelkrim, J. (1) Allain, D. (1) Andrieux, A. (1) Azpilicueta, M. M. (1) Bardin, M. (1) Baudry, E. (1) Berville, A. (1) Bon, M.C. (1) Bonhomme, C. (1) Bouvret, M.E. (1) Breton, C. (1) Mais... Palavra-chave MICROSATELLITES (20) PEST (4) GENE FLOW (3) NULL ALLELES (3) ORTHOPTERA (3) POLYMORPHISME (3) CHENE SESSILE (2) CRIQUET MIGRATEUR (2) ESPECE INVASIVE (2) INTROGRESSION (2) LOCUSTA MIGRATORIA (2) MARQUEUR GENETIQUE LOCUST (2) MULTIPLEX PCR (2) STRUCTURE GENETIQUE (2) ABEILLE HONEY BEE (1) ADN CHLOROPLASTIQUE (1) ALBINO (1) ALEURODE B- AND Q-BIOTYPES (1) ALLÈLE (1) AMPLIFICATION DE GÈNES NOTHOFAGUS (1) Mais... Tipo do documento Journal Article (17) Thesis (2) Conference Paper (1) Ano 2010 (1) 2009 (3) 2008 (7) 2007 (1) 2006 (2) 2005 (3) 2004 (1) 1998 (2) Mais... País France (20) Idioma Inglês (17) Francês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 20 Primeira ... 1 ... Última A first-generation microsatellite-based integrated genetic and cytogenetic map for the European rabbit (Oryctolagus Cuniculus) and localization of angora and albino Inra Chantry-Darmon, C.; Urien, C.; De Rochambeau, H.; Allain, D.; Pena-Arnaud, B.; Hayes, H.; Grohs, C.; Cribiu, E.; Deretz, S.; Larzul, C.; Save, J.C.; Neau, A.; Chardon, P.; Rogel-Gaillard, C.. Summary : Although the European rabbit (Oryctolagus cuniculus) is used both in agronomics and in research, genomic resources for this species are still limited and no microsatellite-based genetic map has been reported. Our aim was to construct a rabbit genetic map with cytogenetically mapped microsatellites so as to build an integrated genetic and cytogenetic map. A reference population of 187 rabbits comprising eight three-generation families with 10–25 offspring per family was produced. One hundred and ninety-four of 305 previouslyidentified microsatellites were included in this study. Of these, 158 were polymorphic with two to seven alleles. The map reported here comprises 111 markers, including 104 INRA microsatellites, five microsatellites from... Tipo: Journal Article Palavras-chave: ALBINO; ANGORA; CHROMOSOMES; CYTOGENETIC MAP; GENETIC MAP; LINKAGE; MICROSATELLITES; RABBIT. Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008246a1ad2&uri=/notices/prodinra1/2008/03/ Characterization and PCR multiplexing of polymorphic microsatellite loci for the invasive ant Wasmannia auropunctata Inra Fournier, D.; Foucaud, J.; Loiseau, A.; Cros-Arteil, S.; Jourdan, H.; Orivel, J.; Le Breton, J.; Chazeau, J.; Dejean, A.; Keller, L.; Estoup, A.. Highly polymorphic genetic markers provide a useful tool for estimating genetic parametersin studies of the evolution of sociality in insects. We isolated and characterized 12 polymorphicmicrosatellite loci in the invasive ant, Wasmannia auropunctata, and described experimental conditions for PCR (polymerase chain reaction) multiplexing and simultaneously genotyping these loci in two sets of five and seven markers. The number of alleles per locus ranged from two to 14 and the observed heterozygosity ranged from 0.233 to 0.967. Moreover, results of cross-species amplification tests are reported in three other species of Wasmannia and in two species of the genus Allomerus Tipo: Journal Article Palavras-chave: STRUCTURE GENETIQUE; POLYMORPHISME ANTS; BLEPHARIDATTINI; ESPECE ENVAHISSANTE; MICROSATELLITES; MYRMICINAE; PEST. Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20074ea97322&uri=/notices/prodinra1/2007/08/ Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria Inra Chapuis, M.-P.; Loiseau, A.; Michalakis, Y.; Lecoq, M.. Because of the scarcity of polymorphic genetic markers available in locust species, only a few population genetics studies have been carried out on this taxon. We isolated and characterized 11 polymorphic microsatellite loci in the pest locust Locusta migratoria capito, and described experimental conditions for polymerase chain reaction (PCR) multiplexing and simultaneously genotyping these loci. The number of alleles per locus ranged from six to 25, and the expected heterozygosity ranged from 0.431 to 0.957. Results of cross-taxon amplification tests are reported in six other Locusta migratoria subspecies, six species of the Oedipodinae subfamily and two other pest locust species Tipo: Journal Article Palavras-chave: CRIQUET MIGRATEUR; POLYMORPHISME; MARQUEUR GENETIQUE LOCUST; LOCUSTA MIGRATORIA; MICROSATELLITES; NULL ALLELES; ORTHOPTERA; PEST. Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200769fce925&uri=/notices/prodinra1/2007/08/ Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria (vol 5, pg 554, 2005) - corrigendum Inra Chapuis, M.P.; Loiseau, A.; Michalakis, Y.; Lecoq, M.; Estoup, A.. Because of the scarcity of polymorphic genetic markers available in locust species, only a few population genetics studies have been carried out on this taxon. We isolated and characterized 11 polymorphic microsatellite loci in the pest locust Locusta migratoria capito, and described experimental conditions for polymerase chain reaction (PCR) multiplexing and simultaneously genotyping these loci. The number of alleles per locus ranged from six to 25, and the expected heterozygosity ranged from 0.431 to 0.957. Results of cross-taxon amplification tests are reported in six other Locusta migratoria subspecies, six species of the Oedipodinae subfamily and two other pest locust species Tipo: Journal Article Palavras-chave: CRIQUET MIGRATEUR; POLYMORPHISME; MARQUEUR GENETIQUE LOCUST; LOCUSTA MIGRATORIA; MICROSATELLITES; NULL ALLELES; ORTHOPTERA; PEST. Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011bec98eb7&uri=/notices/prodinra1/2011/06/ Characterization of 41 microsatellite loci developed from the genome sequence of the poplar rust fungus, Melampsora larici-populina Inra Xhaard, C.; Andrieux, A.; Halkett, F.; Frey, P.. Forty-one microsatellite markers were developed for the poplar rust fungus Melampsora larici-populina. The genome sequence of M. larici-populina strain 98AG31 was screened in silico for microsatellite loci using Magellan software. Thirty-nine loci, evaluated on a sexual population of 35 M. larici-populina isolates sampled on larch trees in the French Alps, were identified to be highly polymorphic. These markers revealed two to 34 alleles, with an average of 7.23 alleles per locus. The estimated heterozygosity ranged from 0.029 to 0.958 across the 41 microsatellite loci. These markers will be highly useful for population genetics studies of M. larici-populina. Tipo: Journal Article Palavras-chave: BASIDIOMYCETE; MELAMPSORA LARICI-POPULINA; POPLAR RUST FUNGUS; GENOME SEQUENCE; MICROSATELLITES; M13-TAILED PRIMER METHOD. Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010e8fd96fd&uri=/notices/prodinra1/2010/10/ Comparison between classical and Bayesian methods to investigate the history of olive cultivars using SSR-polymorphisms Inra Breton, C.; Pinatel, C.; Médail, F.; Bonhomme, C.; Berville, A.. Olive cultivar origins are unknown. We attempted to use multivariate analyses and based Bayesian methods to look for sites of olive cultivar geographic origins. In this study, to establish the method, forty cultivars were chosen with different chlorotypes that represent the olive RAPD-based diversity in the Mediterranean basin. We constructed a dendrogram, as reference, using molecular SSR data. Cultivar origins in the oleaster were examined by using Bayesian clustering methods. It was calculated whether some cultivars may be grouped by admixture analysis to these GRPs and how much olive cultivars admixed to the previously defined oleaster GRPs: two in the east and four in the west of the Mediterranean basin. The comparison between dendrogram and Bayesian... Tipo: Journal Article Palavras-chave: ASSIGNATION (BAYESIAN) METHOD DOMESTICATION; GENETIC RELATIONSHIPS; OLIVE CULTIVARS; MICROSATELLITES. Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200952d38b74&uri=/notices/prodinra1/2009/03/ Dynamique d'hybridation dans le complexe d'espèces des chênes blancs européens (Chênes pédonculés - Quercus robur L., sessiles - Q. petraea (Matt.) Liebl., pubescents -Q. pubescens Willd. et tauzins - Q. pyrenaica Willd.) Inra Lepais, O.. L’hybridation est un processus aux conséquences diverses sur l’évolution des espèces qui est difficile à étudier lorsque les espèces se distinguent mal au niveau morphologique. Afin de comprendre le rôle de l'hybridation dans l’évolution du complexe d’espèces des chênes blancs européens, nous avons utilisé des outils de la génétique des populations pour quantifier les flux de gènes interspécifiques contemporains et étudier le système de reproduction de quatre espèces. Un protocole d'analyse génétique rapide a été développé et des méthodes d’assignations génétiques, permettant de déterminer l’espèce de chaque arbre et d’identifier les hybrides, ont été testées par simulations. Cette méthode a été appliquée en populations naturelles révélant un pourcentage... Tipo: Thesis Palavras-chave: HYBRIDATION; INTROGRESSION; DELIMITATION D'ESPECES; ASSIGNATION GENETIQUE; ANALYSE DE PATERNITE; MICROSATELLITES; CHENES; CHENE SESSILE; CHENE TAUZIN; CHENE BLANC EUROPEEN; ANALYSE GENETIQUE QUERCUS; QUERCUS PETRAEA; QUERCUS PYRENAICA. Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200838ff888d&uri=/notices/prodinra1/2008/11/ Eight polymorphic microsatellite loci for the Australian plague locust, Chortoicetes terminifera Inra Chapuis, M.P.; Popple, J.A.; Simpson, S.J.; Estoup, A.; Martin, J.F.; Steinbauer, M.; McCulloch, L.; Sword, G.A.. Few population genetics studies have been carried out on major locust species. In particular, an understanding of the population genetic structure of the Australian plague locust, Chortoicetes terminifera, is lacking. We isolated and characterized eight polymorphic microsatellite loci in C. terminifera, and described experimental conditions for polymerase chain reaction multiplexing and genotyping these loci. The number of alleles per locus ranged from 11 to 29 and the expected heterozygosity ranged from 0.797 to 0.977. One locus was found to be X-linked. Results of cross-taxon amplification tests are reported in four species of the Oedipodinae subfamily Tipo: Journal Article Palavras-chave: CRIQUET PELERIN; ESPECE INVASIVE CHORTOICETES TERMINIFERA; LOCUST; MICROSATELLITES; NULL ALLELES; ORTHOPTERA; PEST. Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009f0f82bce&uri=/notices/prodinra1/2010/02/ Étude comparée de la structuration génétique des chênes à l’échelle d’un paysage Inra Lepais, O.. Une grande partie des forêts européennes est dominée par les chênes (genre Quercus). Ces espèces ont longtemps été étudiées d’un point de vue taxonomique et écologique. Récemment, l’étude du polymorphisme de l’ADN chloroplastique a permis de mieux comprendre la taxonomie du genre ainsi que l’histoire de la recolonisation postglaciaire de ces espèces. Des flux de gènes intenses entre espèces ont été mis en évidence grâce au développement de marqueurs nucléaires très polymorphes (microsatellites). Cette caractéristique des chênes à s’hybrider entre espèces a conduit à les considérer comme appartenant à un complexe d’espèces. La zone étudiée ici est située dans le canton d’Aurignac (Haute Garonne). Elle se distingue par son étendue relativement restreinte... Tipo: Thesis Palavras-chave: ADN CHLOROPLASTIQUE; MICROSATELLITES; DIFFERENCIATION GENETIQUE INTERSPECIFIQUE; CHENE SESSILE; RECOLONISATION POST-GLACIAIRE QUERCUS. Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20089ecd6c4d&uri=/notices/prodinra1/2008/10/ Genetic diversity of the west European honey bee (Apis mellifera mellifera and A. m. iberia). II.Microsatellite loci Inra Garnery, L.; Franck, P.; Baudry, E.; Vautrin, D.; Cornuet, J.M.; Solignac, M.. La variabilité génétique et la différenciation entre populations a été étudiée pour 11 locus microsatellites dans 15 populations de l’abeille ouest européenne. Les deux sous-espèces qui constituent ce rameau (Apis mellifera mellifera et A. m. iberica) ont une variabilité génétique plus réduite que la plupart des autres populations étudiées avec les mêmes outils moléculaires et plusieurs tests statistiques sont révélateurs d’une réduction récente de leur effectif. De plus, les profils génétiques des populations sont très comparables, du sud de l’Espagne à la Scandinavie, Les populations françaises sont plus ou moins introgressées (de quelques pourcentages jusqu’à 57 %) par des gènes de la lignée nordméditerranéenne, les races ligustica et carnica... Tipo: Journal Article Palavras-chave: ABEILLE HONEY BEE; MICROSATELLITES; POPULATION GENETICS; INTROGRESSION; CONSERVATION. Ano: 1998 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007f04ca494&uri=/notices/prodinra1/2011/02/ Genetic structure and functioning of alien ship rat populations from a Corsican micro-insular complex Inra Abdelkrim, J.; Pascal, M.; Samadi, S.. The detrimental effects of the introduced ship rat on bird species on the Lavezzi Mediterranean archipelago has led to the decision to eradicate the rodent from the main island, Lavezzu (73 ha), as well as from several neighbouring islets. A genetic study using eight microsatellite markers has revealed some of the dynamics of this rat population. First, it has been shown that the rat population was genetically stable (no departure from Hardy-Weinberg equilibrium and no linkage disequilibrium) and as suggested by paleontological data, established for a long time. This information is encouraging in term of viability of the eradication campaign in the long term, even if rare immigration events cannot be excluded. Second, this study shows that rats are likely... Tipo: Journal Article Palavras-chave: INVASION BIOLOGIQUE; ESPECE INVASIVE; ERADICATION; POPULATION ANIMALE; MICROSATELLITE; ILE; MILIEU INSULAIRE SHIP RAT; BIOLOGICAL INVASION; ISLANDS; GENE FLOW; MICROSATELLITES. Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20093edb7feb&uri=/notices/prodinra1/2010/11/ Genetic structure of Botrytis cinerea populations from vegetable greenhouses in France. Abstract Inra Decognet, V.; Bardin, M.; Walker, A.S.; Fermaud, M.; Nicot, P.. Tipo: Conference Paper Palavras-chave: CROPPING SYSTEM; MICROSATELLITES; GENETIC DIVERSITY; GENOTYPE. Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008e753b37a&uri=/notices/prodinra1/2008/05/ Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard Inra Fernandes, L.; Rocheta, M.; Cordeiro, J.; Pereira, S.; Gerber, S.; Oliveira, M.M.; Ribeiro, M.M.. L'apparentement entre parents, la variation de fertilité entre clones et la pollution pollinique font dévier le gain génétique réalisé et la diversité génétique des valeurs attendues en verger à graines de clones à pollinisation libre, en particulier pour une espèce à pollinisation anémophile comme Pinus pinaster Aiton.Ce travail étudie la variation génétique, le système de reproduction et la contamination du pollen d'un verger à graines de clones (VGC) de P. pinaster, en génotypant les 60 clones du VGC et les graines récoltées sur 21 arbres-mères pour trois marqueurs microsatellites nucléaires.La diversité attendue s'est révélée similaire mais l'hétérozygotie observée a diminué de 20 % chez les descendants par rapport aux parents. Le taux... Tipo: Journal Article Palavras-chave: VARIATION GENETIQUE; SYSTEME DE REPRODUCTION; FLUX DE GENES; MICROSATELLITES; PIN MARITIME; VERGER A GRAINES DE CLONES; MARQUEUR GENETIC VARIATION; MATING SYSTEM; GENE FLOW; SIMPLE SEQUENCE REPEATS; MARITIME PINE. Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200892079bd0&uri=/notices/prodinra1/2009/11/ Genome-wide effects of postglacial colonization in Arabidopsis lyrata Inra Muller, M.H.; Leppala, J.; Savolainen, O.. The perennial outcrossing Arabidopsis lyrata is becoming a plant model species for molecular ecology and evolution. However, its evolutionary history, and especially the impact of the climatic oscillations of the Pleistocene on its genetic diversity and population structure, is not well known. We analyzed the broad-scale population structure of the species based on microsatellite variation at 22 loci. A wide sample in Europe revealed that glaciations and postglacial colonization have caused high divergence and high variation in variability between populations. Colonization from Central Europe to Iceland and Scandinavia was associated with a strong decrease of genetic diversity from South to North. On the other hand, the Russian population included in our... Tipo: Journal Article Palavras-chave: MICROSATELLITES; POSTGLACIAL HISTORY; POPULATION STRUCTURE. Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200996da2027&uri=/notices/prodinra1/2009/03/ Isolation and characterization of microsatellite loci from the entomopathogenic hyphomycete, Paecilomyces fumosoroseus Inra Dalleau-Clouet, C.; Gauthier, N.; Fargues, J.; RISTERUCCI, A.M.; Bon, M.C.. Nine microsatellite markers were isolated from the entomopathogenic haploid fungus Paecilomyces fumosoroseus. Genetic diversity was assessed in 26P. fumosoroseus isolates originated from the whitefly Bemisia tabaci collected in various geoclimatic areas. Eight loci were polymorphic with an observed number of alleles ranging from two to six. The loci differentiated some isolates and group of isolates according to their geographical location, showing promise for the study of gene flow. All loci failed to give clear amplifications in P. fumosoroseus isolates from hosts other than B. tabaci. These microsatellite markers provide powerful tools for ecological, epidemiological, and population genetic studies Tipo: Journal Article Palavras-chave: POLYMORPHISME GENETIC DIVERSITY; HOST INSECT; HYPHOMYCETES; MICROSATELLITES; PAECILOMYCES FUMOSOROSEUS. Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20077c147831&uri=/notices/prodinra1/2007/08/ Isolation and characterization of microsatellites in the harlequin ladybird Harmonia axyridis (Coleoptera, Coccinellidae) and cross-species amplification within the family Coccinellidae Inra Loiseau, A.; Malausa, T.; Lombaert, E.; Martin, J.F.; Estoup, A.. A total of 18 microsatellite DNA loci were isolated and characterized from the harlequin ladybird, Harmonia axyridis (Coleoptera: Coccinellidae). We optimized a multiplex panel consisting of two polymerase chain reactions, allowing the genotyping of all loci. The number of alleles and heterozygosity observed at each locus ranged from 1 to 12 and from 0 to 100%, respectively. After Bonferroni correction for multiple tests, none of the loci deviated significantly from Hardy-Weinberg equilibrium and there was no indication of significant linkage disequilibrium among pairs of loci. Successful cross-species amplification was obtained for only three of the seven tested species of Coccinellidae Tipo: Journal Article Palavras-chave: COCCINELLE; COLEOPTERE; ESPECE INVASIVE; MICROSATELLITE BIOLOGICAL INVASION; INSECT; INVASIVE SPECIES; MICROSATELLITES; NUCLEAR MARKER. Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200946b6d228&uri=/notices/prodinra1/2010/08/ New polymorphic microsatellites loci, cross-species amplification and PCR multiplexing in the black Aphid, Aphis fabae Scopoli Inra Gauffre, B.; Coeur D'Acier, A.. Aphis fabae includes four morphological cryptic subspecies, which are mostly identified by their partially distinct secondary host range. To determine the extent of gene flow and isolation between these four taxa, we isolated and characterized 12 microsatellite loci from Aphis fabae fabae and tested cross-species amplification of eight loci from the closely related species Aphis gossypii . Using eight previously described microsatellite loci, we have developed the polymerase chain reaction (PCR) multiplexing of 24 loci, which were separated in tree sets and five PCRs. These sets of microsatellite loci provide high throughput capacity for large-scale population genetic studies at a minimum cost Tipo: Journal Article Palavras-chave: PUCERON; MICROSATELLITE APHIDS; APHIS FABAE COMPLEX; CROSS-SPECIES AMPLIFICATION; HOST PLANT; MICROSATELLITES; MULTIPLEX PCR. Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20091863c639&uri=/notices/prodinra1/2010/03/ Organisation spatiale de la diversité génétique et flux polliniques dans une chênaie mixte Inra Streiff, R.; Ducousso, A.; Kremer, A.. L’organisation de la diversité génétique a été décrite dans le cas d’un peuplement mélangé de chêne sessile et pédonculé constitué de 355 arbres adultes répartis sur une surface de 6 ha. La diversité a été appréhendée à l’aide de 6 loci microsatellites manifestant un polymorphisme très élevé (de 15 à 32 allèles par locus). L'organisation a été décrite à l’aide de deux paramètres : la différenciation (Fst) entre différentes subdivisions spatiales de la parcelle étudiée et l’autocorrélation spatiale (I) entre les génotypes présents sur la parcelle. Les deux espèces se caractérisent par une différenciation génétique faible mais significative, quelle que soit la taille des subdivisions spatiales considérées (à l’exception des faibles tailles < 10 m... Tipo: Journal Article Palavras-chave: DIFFERENCIATION GENETIQUE; AUTOCORRELATION SPATIALE; RECHERCHE DE PATERNITE; FLUX DE POLLEN; MICROSATELLITES. Ano: 1998 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007900ed2a7&uri=/notices/prodinra1/2007/08/ Primer note: A new set of highly polymorphic nuclear microsatellite markers for Nothofagus nervosa and related South American species Inra Marchelli, P.; Caron, H.; Azpilicueta, M. M.; Gallo, L. A.. Nothofagus is the main component of southern South American temperate forests. Overexploitation in the past has led to the loss of 40% of the original distribution range. Genetic diversity as well as biological processes shaping the distribution of the genetic variation (e.g. gene flow) constitutes basic knowledge for the implementation of conservation measures and for the definition of Evolutionary Significant Units. Nuclear microsatellites are the marker of choice for gene flow and fine-scale genetic structure studies. We enlarged a previous set of microsatellites (SSRs) for South American Nothofagus species, with special concern to Nothofagus nervosa (Phil.) Dim. et Mil. Five new SSRs are presented with allele numbers up to 12 in a single population.... Tipo: Journal Article Palavras-chave: STRUCTURE GENETIQUE; POLYMORPHISME; ALLÈLE; AMPLIFICATION DE GÈNES NOTHOFAGUS; MICROSATELLITES; SOUTH AMERICA; GENE FLOW; FINE-SCALE GENETIC STRUCTURE. Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200933c58e92&uri=/notices/prodinra1/2009/03/ Twelve new polymorphic microsatellite loci and PCR multiplexing in the whitefly, Bemisia tabaci Inra Gauthier, N.; Dalleau-Clouet, C.; Bouvret, M.E.. Twelve new dinucleotide microsatellite loci of the whitefly, Bemisia tabaci, were obtained from enriched genomic libraries. Three polymerase chain reaction multiplex sets comprising three, five and four loci were optimized and characterized across 133 B. tabaci females from Israeli rearings and natural populations collected in four Mediterranean countries (Tunisia, France, Spain and Morocco). There were three to 24 alleles per locus and the observed heterozygosity was from 0.084 to 0.420. Deviation from Hardy–Weinberg equilibrium was detected at four loci associated with significant heterozygote deficiencies due to null alleles and presence of subpopulations that were mostly in the Tunisian sample. The 12 loci carried independent information Tipo: Journal Article Palavras-chave: ESPECES INVASIVES; ALEURODE B- AND Q-BIOTYPES; BEMISIA TABACI; INVASIVE PEST; MICROSATELLITES; MULTIPLEX PCR. Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009a729d8cd&uri=/notices/prodinra1/2010/03/ Registros recuperados: 20 Primeira ... 1 ... Última

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