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Registros recuperados: 37
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Antibacterial susceptibility patterns and cross-resistance of methicillin resistant and sensitive Staphyloccus aureus isolated from the hospitalized patients in Shiraz, Iran BJM
Japoni,Aziz; Ziyaeyan,Mazyar; Jmalidoust,Marzieh; Farshad,Shohreh; Alborzi,Abdolvahab; Rafaatpour,Noraladdin; Badiee,Parisa.
Nosocomial infections caused by methicillin-resistant staphylococci (MRSA) pose a serious problem in many countries. This study aimed to determine the antibacterial susceptibility patterns of methicillin sensitive and resistant Staphylococcus aureus isolates from the hospitalized patients. Totally 356 isolates of Staphylococcus aureus (S. aureus) including 200, 137 and 19 corresponding to MSSA, MRSA, and intermediate MRSA strains, respectively were isolated. Antibacterial susceptibility patterns of the isolates to 14 antibiotics were examined using Kirby-Bauer method. MICs of 15 antibiotics to 156 MRSA isolates were determined by E test method. Cross-resistances of MRSA isolates (137+19) to the other tested antibiotics were also determined. S.aureus with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Minimum inhibitory concentration; Empirical therapy.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300005
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Antimicrobial activity of allylic thiocyanates derived from the Morita-Baylis-Hillman reaction BJM
Sá,Marcus Mandolesi; Ferreira,Misael; Lima,Emerson Silva; Santos,Ivanildes dos; Orlandi,Patrícia Puccinelli; Fernandes,Luciano.
Bacterial resistance to commonly used antibiotics has been recognized as a significant global health issue. In this study, we carried out the screening of a family of allylic thiocyanates for their action against a diversity of bacteria and fungi with a view to developing new antimicrobial agents. Allylic thiocyanates bearing halogenated aryl groups, which were readily obtained in two steps from the Morita-Baylis-Hillman adducts, showed moderate-to-high activity against selective pathogens, including a methicillin-resistant S. aureus (MRSA) strain. In particular cases, methyl (Z)-3-(2,4-dichlorophenyl)-2-(thiocyanomethyl)-2-propenoate exhibited antimicrobial activity comparable to the reference antibiotic Imipenem.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial; Allylic thiocyanate; Morita-Baylis-Hillman reaction; MRSA.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300007
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Application of RAPD-PCR for Determining the Clonality of Methicillin Resistant Staphylococcus aureus Isolated from Different Hospitals BABT
Idil,Neslihan; Bilkay,Isil Seyis.
Randomly amplified polymorphic DNA (RAPD)-PCR was applied with ten random 10-mer primers to examine the molecular diversity among methicillin resistant Staphylococcus aureus (MRSA) strains in the hospitals and to investigate the epidemiological spread of these strains between different hospitals. The main objective of the study was to identify appropriate primers, which successfully established the clonality of MRSA. Three of the primers yielded particularly discriminatory patterns and they were used to perform the RAPD analysis which revealed different bands ranging from 200 to 1500 bp. Dendogram was created by the un-weighted pair group method using arithmetic (UPGMA) average clustering and it was constructed based on the combination results of the new...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; RAPD-PCR; Clonal dissemination; UPGMA.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400548
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Characterization of the Brazilian endemic clone of methicillin-resistant Staphylococcus aureus (MRSA) from hospitals throughout Brazil BJID
Oliveira,Geraldo A.; Faria,Juarez B.; Levyand,Carlos E.; Mamizuka,Elsa M..
The objective of this study was to characterize patterns of the Brazilian endemic clone of methicillin-resistant Staphylococcus aureus (MRSA) from hospitals throughout Brazil. We studied 83 MRSA strains isolated from patients hospitalized in 27 public and private hospitals in 19 cities located in 14 Brazilian states from September, 1995, to June, 1997. The MRSA strains were typed using antibiograms, bacteriophage typing and pulsed field gel electrophoresis (PFGE). The analysis of genomic DNA by PFGE showed that 65 isolates presented the same PFGE pattern. This pattern was present in all of the hospitals studied indicating the presence of an endemic MRSA clone widely disseminated throughout Brazilian hospitals (BEC). All isolates belonging to the BEC proved...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococus aureus; MRSA; Epidemiology; Brazilian endemic clone.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000400001
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Conventional versus molecular tests (Multiplex PCR and PCR mecA gene) for detection of methicillin resistant Staphylococcus aureus BJM
Ribas,Rosineide Marques; Gontijo-Filho,Paulo Pinto; Darini,Ana Lúcia da Costa.
In this study, for detection of methicillin-resistant S. aureus (MRSA), a mecA multiplex PCR-based amplification was compared with the 1 µg oxacillin disk diffusion test, detection of minimal inhibitory concentration (MIC), and screening in agar with 4% NaCl and 6 µg/mL oxacillin. Among 24 isolates obtained from blood, mecA gene was detected in only 16 (66.7%) isolates by multiplex PCR. The MIC test showed a range of resistance to oxacillin from 0.19 to 512 µg/mL, among these isolates. Data obtained by screening and dilution tests showed that sensitivity to methicillin was 80.0% and 72.8%, respectively, when compared with the presence of mecA gene (multiplex). All isolates, including the negatives, when revaluated for mecA gene by PCR were positive....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multiplex PCR; MRSA; Beta-lactamase; MecA gene.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500012
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Detecção dos genes de Staphylococcus aureus, enterotoxinas e de resistência à meticilina em leite Arq. Bras. Med. Vet. Zootec.
Dias,N.L.; Silva,D.C.B.; Oliveira,D.C.B.S.; Fonseca Junior,A.A.; Sales,M.L.; Silva,N..
Realizou-se a detecção do gene de Staphylococcus aureus, de enterotoxinas e de resistência à meticilina com extração de DNA feita diretamente de amostras de leite. Das 200 amostras estudadas, 145 (72,5%) amplificaram o gene femA, e estas foram analisadas quanto à presença dos genes sea, seb, sec e mecA. Os genes das enterotoxinas mais prevalentes foram: sea (60%), seb (37,9%) e sec (6,9%). Foram encontradas 18 amostras de leite (11,0 %) com S. aureus portadores do gene mecA. A detecção de S. aureus diretamente do leite, sem a necessidade de isolamento bacteriano e a caracterização do potencial enterotoxigênico, demonstra que a técnica de PCR é muito útil para estudos epidemiológicos das infecções estafilocócicas da glândula mamária. O alto percentual...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Enterotoxinas; MRSA; PCR.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000600036
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Detection of methicillin resistance in Staphylococcus aureus isolated from pediatric patients: is the cefoxitin disk diffusion test accurate enough? BJID
Mimica,M.J.; Berezin,E.N.; Carvalho,R.L.B.; Mimica,I.M.; Mimica,L.M.J.; Sáfadi,M.A.P.; Schneider,E.; Caiaffa-Filho,H.H..
We evaluated the performance of several methods for the detection of methicillin resistance in Staphylococcus aureus using 101 clinical S. aureus isolates from pediatric patients in a tertiary hospital in Brazil; 50 isolates were mecA-positive and 51 were mecA-negative. The Etest and oxacillin agar screening plates were 100% sensitive and specific for mecA presence. Oxacillin and cefoxitin disks gave sensitivities of 96 and 92%, respectively, and 98% specificity. Alterations of CLSI cefoxitin breakpoints increased sensitivity to 98%, without decreasing specificity. Our results highlight the importance of a continuing evaluation of the recommended microbiological methods by different laboratories and in different settings. If necessary, laboratories should...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Disc diffusion; Cefoxitin; MecA.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000400009
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Detection of the mecA gene and identification of Staphylococcus directly from blood culture bottles by multiplex polymerase chain reaction BJID
Rocchetti,Taisa Trevizani; Martins,Katheryne Benini; Martins,Patricia Yoshida Faccioli; Oliveira,Rogério Antonio de; Mondelli,Alessandro Lia; Fortaleza,Carlos Magno Castelo Branco; Cunha,Maria de Lourdes Ribeiro de Souza da.
ABSTRACT Introduction: Staphylococcus spp. – both S. aureus, including methicillin-resistant strains (MRSA) and coagulase negative staphylococci (CoNS) – are relevant agents of healthcare-associated infections. Therefore, the rapid recognition of MRSA and methicillin-resistant CoNS from blood stream infections is critically important for patient management. It is worth noting that inappropriate empiric therapy has been associated with higher in-hospital mortality. Material and methods: In this study we evaluated a multiplex polymerase chain reaction (multiplex PCR) standardized to detect Staphylococcus spp., S. aureus, and mecA gene-encoded oxacillin resistance directly from blood culture bottles. A total of 371 blood cultures with Gram-positive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus spp.; Staphylococcus aureus; MRSA; MecA gene; Blood cultures; Multiplex PCR.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200099
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Diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus in Latin America BJID
Zurita,Jeannete; Mejía,Carlos; Guzmán-Blanco,Manuel.
Strategies to monitor and control the spread of methicillin-resistant Staphylococcus aureus (MRSA) infections are dependent on accurate and timely diagnosis of MRSA in both hospital and community settings. In Latin America, significant diversity in diagnostic and susceptibility testing procedures exists at the regional, national and local levels. Various tests for S. aureus and MRSA are available in clinical settings, but the application of these techniques differs between and within countries, and quality control measures are not uniformly applied to verify diagnoses. To optimize the diagnosis of MRSA infections across Latin America, a more consistent approach is required. This may include: adoption and appropriate adaption of specific guidelines for MRSA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Diagnosis; Susceptibility testing; Latin America.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000800005
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Efficacy of a program of prevention and control for methicillin-resistant Staphylococcus aureus infections in an intensive-care unit BJID
Moreira,Marina; Freitas,Marise R.; Martins,Sinaida T.; Castelo,Adauto; Medeiros,Eduardo Alexandrino Servolo.
Methicillin-resistant Staphylococcus aureus (MRSA) is endemic in most Brazilian Hospitals, and there are few studies which show the efficacy of control measures in such situations. This study evaluated intensive care unit (ICU) patients, in two years divided in control, intervention and post-intervention group. Intervention measures: hands-on educational programs for healthcare workers; early identification of MRSA infected or colonized patients, labeled with a bed-identification tag for contact isolation; nasal carriers, patients, and healthcare professionals treated with topical mupirocin for five days. The hospital infection rates in the control period were compared to the ones in the post-intervention period. Hospital infection rates were found by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Methicillin-resistant Staphylococcus aureus; Intensive care unit infections.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100015
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Emergence of clonal complex 5 (CC5) methicillin-resistant Staphylococcus aureus (MRSA) isolates susceptible to trimethoprim-sulfamethoxazole in a Brazilian hospital BJMBR
Teixeira,M.M.; Araújo,M.C.; Silva-Carvalho,M.C.; Beltrame,C.O.; Oliveira,C.C.H.B.; Figueiredo,A.M.S.; Oliveira,A.G..
In this study, genotyping techniques including staphylococcal chromosomal cassette mec (SCCmec) typing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and restriction-modification tests were used to compare the molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered at two times within a 10-year interval (1998 and 2008) from a tertiary Brazilian hospital. In addition, the antimicrobial susceptibility profiles were analyzed. All 48 MRSA isolates from 1998 and 85.7% from 2008 (48/56 isolates) displayed multidrug-resistance phenotypes and SCCmec III. All but one of the 13 representative SCCmec III isolates belonged to CC8 and had PFGE patterns similar to that of the BMB9393 strain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Antimicrobial susceptibility; CC5; Cordobes clone; Trimethoprim-sulfamethoxazole.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700011
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Epidemiology and surveillance of methicillin-resistant staphylococcus aureus in Latin America BJID
Mejía,Carlos; Zurita,Jeannete; Guzmán-Blanco,Manuel.
Surveillance systems monitoring the spread and divergence of methicillin-resistant Staphylococcus aureus (MRSA) strains are critical if preventive and therapeutic measures targeting MRSA infection are to be employed optimally. Surveillance provides information on the spread of MRSA, on the emergence of new strains within hospitals and communities, on the antibiotic resistance profile and virulence of strains, and on the risk factors associated with infection. These data help clinicians to provide appropriate empiric treatment of infections circulating in their region, leading to improved patient outcomes. While information on MRSA epidemiology in Latin America is growing, significant gaps exist in the available data, especially in local areas where fewer...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Epidemiology surveillance Latin America resistance.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000800003
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Epidemiology of diabetic foot infections in a reference tertiary hospital in India BJM
Saseedharan,Sanjith; Sahu,Manisa; Chaddha,Roonam; Pathrose,Edwin; Bal,Arun; Bhalekar,Pallavi; Sekar,Priyadharshini; Krishnan,Padma.
Abstract Introduction The present study attempts to examine the microbial profile and antibiotic susceptibility of diabetic foot infections in the intensive care unit of a tertiary referral centre for diabetic foot. As part of the study, we also attempted to find the prevalence of blaNDM-like gene among carbapenem-resistant gram negative infections. Methodology A prospective study of 261 patients with diabetic foot infections was performed during the period between January 2014 and June 2014. Results A total of 289 isolates were obtained from 178 tissue samples from 261 patients, 156 (59.7%) males and 105 (40.2%) females, with a mean age of 58 years (-15 years), having diabetic foot infection. No growth was seen in thirty eight (17.6%) tissue samples....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diabetic foot infection; BlaNDM; MRSA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200401
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Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil BJM
Sousa Júnior,Francisco Canindé de; Néri,Gildelane da Silva; Silva,Ana Karine; Araújo,Bibiana Priscila Rodrigues Câmara de; Guerra,Myrian Júlia de Paiva Dourado; Fernandes,Maria José de Britto Costa; Milan,Eveline Pipolo; Melo,Maria Celeste Nunes de.
Many methods have been described for the detection of methicillin-resistant Staphylococcus aureus (MRSA), but the heterogeneous expression of methicillin resistance affects the reliability of these methods. The aim of the present study was to evaluate some methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil. Among the isolates, 15 were methicillin-susceptible and 45 were methicillin-resistant, including low-level heterogeneous resistance strains. Both the 30 µg-cefoxitin disk and PBP2a test had 100% sensibility/specificity and appear to be good options for the detection of MRSA in the clinical laboratory.
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Methicillin resistance; Heterogeneous resistance; Cefoxitin; PBP2a.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200009
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Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil BJMBR
Sousa-Junior,F.C. de; Silva-Carvalho,M.C.; Fernandes,M.J.B.C.; Vieira,M.F.P.; Pellegrino,F.L.P.C.; Figueiredo,A.M.S.; Melo,M.C.N. de; Milan,E.P..
Methicillin-resistant Staphylococcus aureus (MRSA) is a major agent of hospital infections worldwide. In Brazil, a multiresistant MRSA lineage (ST239-SCCmecIIIA), the so-called Brazilian epidemic clone (BEC), has predominated in all regions. However, an increase in nosocomial infections caused by non-multiresistant MRSA clones has recently been observed. In the present study, 45 clinical isolates of MRSA obtained from a university hospital located in Natal city, Brazil, were identified by standard laboratory methods and molecularly characterized using staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis. Antimicrobial susceptibility testing was carried out using CLSI methods. The MRSA isolates studied displayed a...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Methicillin-resistant Staphylococcus aureus; MRSA; Antimicrobial resistance; Brazilian epidemic clone; Pediatric clone; SCCmecIV.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000002
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Identification of Methicillin-Resistant Staphylococcus aureus in Bulk Tank Milk Ciênc. Tecnol. Aliment.
KEYVAN,Erhan; YURDAKUL,Ozen; DEMIRTAS,Ahu; YALCIN,Halil; BILGEN,Nuket.
Abstract Staphylococcus aureus is the leading cause of intoxications in humans. Also, methicillin-resistant S. aureus (MRSA) is an emerging problem in food-producing animals. The presence of MRSA in milk may create a risk to public health. The aim of this study was to evaluate the presence of MRSA in bulk tank milk samples. One hundred and twenty bulk tank milk samples were analyzed using the convenient sampling method. The isolates were confirmed by real-time polymerase chain reaction (RT-PCR) targeting nuc and mecA gene in S. aureus. Antibiotic resistance profiles of the isolates were tested by disc agar diffusion method. In the current study, S. aureus was detected in 44 (36.66%) of the samples. The presence of the mecA gene was found to be positive in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibiotic resistance; Bulk tank milk; MRSA.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100150
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In vitro susceptibility of methicillin-resistant Staphylococcus aureus isolates from skin and soft tissue infections to vancomycin, daptomycin, linezolid and tedizolid BJID
Múnera,Johanna Marcela Vanegas; Ríos,Ana María Ocampo; Urrego,Daniela Montoya; Jiménez Quiceno,Judy Natalia.
Abstract Introduction Treatment of multidrug-resistant Gram-positive infections caused by Staphylococcus aureus remains as a clinical challenge due to emergence of new resistance mechanisms. Tedizolid is a next-generation oxazolidinone, recently approved for skin and soft tissues infections. We conducted a study to determine in vitro susceptibility to vancomycin, daptomycin, linezolid and tedizolid in MRSA clinical isolates from adult patients with skin and soft tissue infections. Material and methods Methicillin-resistant S. aureus isolates were collected in three tertiary-care hospitals of Medellin, Colombia, from February 2008 to June 2010 as part of a previous study. Clinical characteristics were assessed by medical records and MIC values were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Skin and soft tissues infections; Tedizolid; Linezolid; Vancomycin; Daptomycin.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500493
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Letter to the Editor: Retrospective multicenter study reveals absence of MRSA-associated bovine mastitis in Brazil (1994 to 2016). Repositório Alice
AIZAWA, J.; SOUZA-FILHO, A. F.; GUIMARÃES, A. S.; VASCONCELOS, C. G. C.; BRITO, M. A. V. P.; SELLERA, F. P.; CORTEZ, A.; HEINEMANN, M. B..
Tipo: Artigo de periódico Palavras-chave: MRSA; Resistance to methicillin; Staphylococcus Aureus.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118703
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Local circulating clones of Staphylococcus aureus in Ecuador BJID
Zurita,Jeannete; Barba,Pedro; Ortega-Paredes,David; Mora,Marcelo; Rivadeneira,Sebastián.
ABSTRACT The spread of pandemic Staphylococcus aureus clones, mainly methicillin-resistant S. aureus (MRSA), must be kept under surveillance to assemble an accurate, local epidemiological analysis. In Ecuador, the prevalence of the USA300 Latin American variant clone (USA300-LV) is well known; however, there is little information about other circulating clones. The aim of this work was to identify the sequence types (ST) using a Multiple-Locus Variable number tandem repeat Analysis 14-locus genotyping approach. We analyzed 132 S. aureus strains that were recovered from 2005 to 2013 and isolated in several clinical settings in Quito, Ecuador. MRSA isolates composed 46.97% (62/132) of the study population. Within MRSA, 37 isolates were related to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; MSSA; Epidemic clones; MLVA typing.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600525
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Mannitol-negative methicillin-resistant Staphylococcus aureus from nasal swab specimens in Brazil BJM
Santos,Danielle Caldeira Martins dos; Costa,Thaina Miranda da; Rabello,Renata Fernandes; Alves,Fábio Aguiar; Mondino,Silvia Susana Bona de.
The isolation of mannitol-negative methicillin-resistant Staphylococcus aureus from nasal swabs is reported. Among the 59 isolates, 9 (15%) isolates were mannitol-negative; all of these isolates were categorized as staphylococcal cassette chromosome mec (SCCmec) type IVa. This report emphasizes that mannitol fermentation on mannitol salt agar should not be used as the sole criterion when screening nasal swab specimens for S. aureus.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Mannitol-negative; MRSA; Nasal swab surveillance; SCCmec.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200531
Registros recuperados: 37
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