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| SOUSA, A. L. M. de. |
| Resumo: As Lentiviroses de Pequenos Ruminantes causam infecções crônicas em caprinos e ovinos, podendo ocasionarmastite, artrite, emagrecimento progressivo, pneumonia e problemas neurológicos. Estas enfermidades têm como principal via de transmissão a ingestão de leite e colostro oriundo de animaisinfectados, além de possuírem um alto índice de contágio e permanência em rebanhos a nível mundial. Vale ressaltar que, até o momento, não há tratamentos e/ou vacinas eficazes e disponíveis,necessitando, assim, a busca de meios inovadores passíveis de serem implementados no combate a estas enfermidades. Atualmente, vem se desenvolvendo pesquisas para o uso deantivirais tanto de origem sintética, como o dodecil sulfato de sódio (SDS), como de origem fitoquímica,... |
| Tipo: Teses |
Palavras-chave: Antivirals; Chemical additive; Viral replication; Meliáceas; Antiviral; Aditivo químico; LVPR; CAEV; Maedi-Visna; MVV; Pequeno ruminante; Caprino; Leite de Cabra; Azadirachta Indica; Nim; Melia Azedarach; Cinamomo; Planta Produtora de Pesticida; Extrato Vegetal; Goat milk; Goats; Disease transmission; Lentivirus; Small ruminants; Plant extracts; Visna maedi virus. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125033 |
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| Marinho,Rebeca C.; Martins,Gabrielle R.; Souza,Kelma C.; Sousa,Ana Lídia M.; Silva,Sabrina Tainah C.; Nobre,Juliana A.; Teixeira,Maria F.S.. |
| Abstract Small ruminant lentiviruses (SRLV) have high genetic variability which results in different viral strains around the world. This create a challenge to design sensible primers for molecular diagnosis in different regions. This work proposes a protocol of duplex nested-PCR for the precise diagnosis of SRLV. The technique was designed and tested with the control strains CAEV Co and MVV 1514. Then, field strains were submitted to the same protocol of duplex nested-PCR. Blood samples of sheep and goats were tested with AGID and nested PCR with specific primers for pol, gag and LTR. The AGID results showed low detection capacity of positive animals, while the nested PCR demonstrated a greater capacity of virus detection. Results demonstrated that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Standardization; Multiplex; Viruses; CAEV; MVV. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500083 |
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| Martins,Gabrielle R.; Marinho,Rebeca C.; Bezerra Junior,Rosivaldo Q.; Alves,Antoniel de O.; Câmara,Lilia M.C.; Albuquerque-Pinto,Luiz C.; Teixeira,Maria F. da S.. |
| Abstract Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibroblast primary culture. A new alternative type of host cells, derived from goat umbilical cord, was isolated and characterized phenotypically with its main purpose being to obtain cell monolayers that could be used for the diagnosis and isolation of small ruminant lentiviruses in cell culture. To accomplish this goal, cells were isolated from umbilical cords; characterized phenotypically by flow cytometry analysis; differentiate into osteogenic,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CAEV; MVV; Cell culture; MSC; Flow cytometry. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100125 |
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| ALVES, S. M.; TEIXEIRA, M. F. da S.; PINHEIRO, R. R.; ALVES, F. S. F.; LIMA, A. M. C.; FARIAS, D. A. de; SANTOS, V. W. S. dos; AZEVEDO, D. A. A. de; MARTINS, G. R.; AGUIAR, T. D. de F.. |
| Abstract: The production performance of a livestock herd can be compromised by various diseases. In sheep, maedi-visna (MV) infections, which have a chronic nature, are caused by a virus (maedi-visna virus (MVV)) belonging to the genus Lentivirus of the Retroviridae family. The infection can cause significant economic losses and has considerable health impacts on sheep breeding in production systems. Due to the importance of this disease in sheep flocks, the objective was to conduct a serosurvey of MVV in the states of Ceará (CE), Rio Grande do Norte (RN), Paraíba (PB), and Sergipe (SE). A total of, 3332 serum samples were collected in the four states, 1011 in CE, 931 in RN, 459 in PB, and 931 in SE, with the number of samples proportional to the actual... |
| Tipo: Artigo de periódico |
Palavras-chave: Enfermidade; Sorologia; Maedi-visna; Lentivirose; MVV; Ovino; Doença Animal; Anticorpo; Sheep diseases; Visna maedi virus; Serology; Lentivirus; Viral antibodies; Infection; Animal diseases. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1096224 |
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| SOUSA, M. S.; RÊGO, W. M. F. do; SANTOS, R. L. dos; ALVES, F. S. F.; PINHEIRO, R. R.; FARIAS, D. A. de; SANTIAGO, L. B.; DINIZ, B. L. M.; CARDOSO, J. de F. S.; PAULA, N. R. de O.. |
| Resumo: Objetivando estimar a prevalência dos Lentivírus de Pequenos Ruminantes (LVPR) em rebanhos ovinos da Micro-Região do Alto-Médio Gurguéia, no sul do estado do Piauí, foram coletados 965 amostras sorológica em 37 propriedades. Para a detecção de anticorpos antilentivírus foi utilizado o teste de Microimudifusão em Gel de Agarose (MIDGA). Os resultados revelaram soroprevalência geral de 0,51% de animais positivos. Portanto, as Lentiviroses de Pequenos Ruminantes estão presentes em rebanhos ovinos da Micro-Região do Alto-Médio Gurguéia, no Sul do Estado do Piauí. Abstract: In order to estimate the prevalence of Small Ruminants lentivirus (SRLV) in sheep flocks at Micro-Region of the Upper-Middle Gurguéia , in the southern state of Piaui, 965 blood... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: MVV; Maed-Visna; Lentivirose.; Ovino; Doença Animal.; Lentivirus; Seroprevalence; Small ruminants; Disease prevalence; Sheep diseases; Brazil.. |
| Ano: 2011 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/909164 |
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| LIMA, C. C. V. de; AYRES, M. C. C.; PINHEIRO, R. R.; COSTA, N. C.; SOUZA, T. S. de; ANDRIOLI, A.; AZEVEDO, D. A. A.; SANTOS, V. W. S. dos. |
| Abstract: This study was conducted to evaluate caprine arthritis encephalitis virus (CAEV) transmission among sheep using 15 lambs that were distributed in 2 experimental groups. The exposed group consisted of 10 lambs that remained with their mothers, who were experimentally infected with CAEV. The non-exposed group was characterized as the control group and was comprised of 5 lambs that remained with their CAEV-negative mothers. Blood samples were collected monthly from birth until 1 year of life. To evaluate the transmission, an agar gel immunodiffusion test (AGID), enzyme immunoassay (ELISA), immunoblotting (IB), and nested polymerase chain reaction (nPCR) techniques were used. The non-exposed group was negative in all of the tests throughout the whole... |
| Tipo: Artigo de periódico |
Palavras-chave: Lentivirose; CAEV; LVPR; MVV; Transmissão intra-espécie; Intra-species transmission; Ovino; Doença Animal; Transmissão de Doença; Cordeiro; Sheep diseases; Lambs; Disease transmission; Caprine arthritis encephalitis virus; Virus transmission; Ovine lentivirus; Maedi-visna disease. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1096081 |
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| Lima,Carla Caroline Valença de; Ayres,Maria Consuêlo Caribé; Pinheiro,Raymundo Rizaldo; Costa,Joselito Nunes; Souza,Thiago Sampaio de; Pinheiro,Alice Andrioli; Azevedo,Dalva Alana Aragão; Santos,Vanderlan Warlington Souza dos. |
| ABSTRACT: This study was conducted to evaluate caprine arthritis encephalitis virus (CAEV) transmission among sheep using 15 lambs that were distributed in 2 experimental groups. The exposed group consisted of 10 lambs that remained with their mothers, who were experimentally infected with CAEV. The non-exposed group was characterized as the control group and was comprised of 5 lambs that remained with their CAEV-negative mothers. Blood samples were collected monthly from birth until 1 year of life. To evaluate the transmission, an agar gel immunodiffusion test (AGID), enzyme immunoassay (ELISA), immunoblotting (IB), and nested polymerase chain reaction (nPCR) techniques were used. The non-exposed group was negative in all of the tests throughout the whole... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lentiviruses; CAEV; MVV; Intra-species transmission; SRLV. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000450 |
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