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Registros recuperados: 5
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Development and activity of Glomus intraradices as affected by co-existence with Glomus claroideum in one root system Inra
Janoušková, M.; Seddas, P.; Mrnka, L.; Van Tuinen, D.; Dvořáčková, A.; Tollot, M.; Gianinazzi-Pearson, V.; Vosátka, M.; Gollotte, A..
The co-existence of two arbuscular mycorrhizal fungal (AMF) species, Glomus intraradices and Glomus claroideum, in the root systems of plants was investigated in a greenhouse experiment aimed at reconstructing interactions during an early stage of primary succession on a coal-mine spoil bank in Central Europe. Two plant species, Tripleurospermum inodorum and Calamagrostis epigejos, were inoculated either with one or both AMF species. Fungal development, determined by trypan blue and alkaline phosphatase staining as well as by PCR amplification of rRNA genes with species-specific primers, and the expression of five genes with different metabolic functions in the intraradical structures of G. intraradices were followed after 6 and 9 weeks of cultivation. The...
Tipo: Journal Article Palavras-chave: MYCORHIZES A ARBUSCULES; PHOSPHATASE ALCALINE; MYCELIUM INTRARADICAL ALKALINE PHOSPHATASE; ARBUSCULAR MYCORRHIZA; GENE EXPRESSION; INTRARADICAL MYCELIUM; NESTED PCR.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20119c7b67f7&uri=/notices/prodinra1/2011/05/
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Localisation et visualisation de transcrits fongiques in situ Une méthode originale qui combine biologie moléculaire et microscopie confocale Inra
Arnould, C.; Tollot, M.; Seddas, P.; Gianinazzi-Pearson, V..
Les Gloméromycètes, formant la symbiose mycorhizienne à arbuscules, sont des champignons biotrophes obligatoires qui sont intimement associés aux tissus végétaux qu’ils colonisent. Les techniques utilisées pour suivre l’expression des gènes fongiques dans les racines mycorhizées ne permettent pas de définir le profil spatio-temporel de leur activité. Afin de tracer l’activité transcriptionnelle fongique dans les racines mycorhizées, une méthode innovante, basée sur la RT-PCR in situ, a donc été développée. Cette technique permet de localiser, en microscopie optique confocale à balayage laser, des transcrits fongiques, à l’aide d’amorces spécifiques marquées par un fluorochrome. Du fait de leur importance biologique et de la facilité de leur détection, nous...
Tipo: Journal Article Palavras-chave: RT-PCR IN SITU; MICROSCOPIE OPTIQUE CONFOCALE; EXPRESSION DE GENE; CHAMPIGNON MYCORHIZE; MYCORHIZES A ARBUSCULES.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011fb0fd6a5&uri=/notices/prodinra1/2011/07/
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Méthode simple pour évaluer le potentiel endomycorhizogène d’un inoculum Inra
Kuszala, C.; Gianinazzi, S..
Les champignons mycorhizogènes à arbuscules (MA), forment une association mutualiste avec la plupart des plantes terrestres. Ils favorisent leur croissance et leur résistance à de nombreux stress biotiques et abiotiques. Leur utilisation en agriculture permet de limiter l’apport d’intrants chimiques. Pour gérer leur population il faut connaître leur abondance dans le sol, et donc évaluer le potentiel mycorhizogène du sol. Pour cela on utilise un test qui mesure le pouvoir de colonisation racinaire de ces symbiotes au niveau d’une plante piège. La méthode du « Most Probable Number » (MPN) est la plus utilisée. L’objectif de cette étude a été de comparer deux techniques d’évaluation du MPN : une technique volumétrique basée sur des dilutions successives et...
Tipo: Journal Article Palavras-chave: MPN; TEST KHIDEUX; CHAMPIGNONS ENDOMYCORHIZOGENES; INOCULUM; MYCORHIZES A ARBUSCULES.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20117863b21d&uri=/notices/prodinra1/2011/07/
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Plant-driven selection of microbes Inra
Hartmann, A.; Schmid, M.; Van Tuinen, D.; Berg, G..
The rhizodeposition of plants dramatically influence the surrounding soil and its microflora. Root exudates have pronounced selective and promoting effects on specific microbial populations which are able to respond with chemotaxis and fast growth responses, such that only a rather small subset of the whole soil microbial diversity is finally colonizing roots successfully. The exudates carbon compounds provide readily available nutrient and energy sources for heterotrophic organisms but also contribute e.g. complexing agents, such as carboxylates, phenols or siderophores for the mobilization and acquisition of rather insoluble minerals. Root exudation can also quite dramatically alter the pH- and redox-milieu in the rhizosphere. In addition, not only...
Tipo: Journal Article Palavras-chave: EXSUDATION RACINAIRE; RHIZODEPOSITION; DIVERITE MICROBIENNE; MYCORHIZES A ARBUSCULES; ANTIMICROBIEN ROOT EXUDATION; RHIZODEPOSITION; MICROBIAL DIVERSITY; RHIZOSPHERE BACTERIA; MYCORRHIZAL FUNGI; ARBUSCULAR MYCORRHIZA; ECTOMYCORRHIZA; ANTIMICROBIALS; SIGNALLING COMPOUNDS; PLANT GROWTH PROMOTION; BIOLOGICAL CONTROL; "BIASED RHIZOSPHERE CONCEPT".
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011720427a9&uri=/notices/prodinra1/2011/05/
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Symbiosis-related pea genes modulate fungal and plant gene expression during the arbuscule stage of mycorrhiza with Glomus intraradices Inra
Kuznetsova, E.; Seddas-Dozolme, P.; Tollot, M.; Van Tuinen, D.; Borisov, A.; Gianinazzi, S.; Gianinazzi-Pearson, V..
The arbuscular mycorrhiza association results from a successful interaction between genomes of the plant and fungal symbiotic partners. In this study, we analyzed the effect of inactivation of late-stage symbiosis-related pea genes on symbiosis-associated fungal and plant molecular responses in order to gain insight into their role in the functional mycorrhizal association. The expression of a subset of ten fungal and eight plant genes, previously reported to be activated during mycorrhiza development, was compared in Glomus intraradices-inoculated wild-type and isogenic genotypes of pea mutated for the PsSym36, PsSym33, and PsSym40 genes where arbuscule formation is inhibited or fungal turnover modulated, respectively. Microdissection was used to...
Tipo: Journal Article Palavras-chave: MYCORHIZES A ARBUSCULES; GLOMUS INTRARADICES.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011268ce933&uri=/notices/prodinra1/2011/05/
Registros recuperados: 5
Primeira ... 1 ... Última
 

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