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| Katarzyna, S; Renault, Tristan; Wolowicz, M. |
| This study presents results of histopathologic, cytogenetics and flow cytometry analyses performed on Macoma balthica collected from the Gulf of Gdansk (Baltic Sea) in 2003 in order to compare the techniques for diagnosis of neoplasia. The proportion of affected clams gave a crude prevalence of 15.7 %. The four stages of the disease defined by histology and three stages of neoplasia defined by flow cytometry were reported. Stage I defined by flow cytometry corresponded to stages I and II defined by histology. Chromosome analysis did not lead to a staging of neoplastic progression. Both cytogenetics and flow cytometry indicated a difference in the DNA content of non-neoplastic and neoplastic cells. Cytogenetics examination marked that the range of... |
| Tipo: Text |
Palavras-chave: Baltic Sea; Cytofluorymetry; Cytogenetics; Histology; Macoma balthica; Neoplasia. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2921.pdf |
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| Smolarz, Kasia; Renault, Tristan; Soletchnik, Patrick; Wolowicz, Macciek. |
| A flow cytometry protocol was applied for the detection of neoplasia in Macoma balthica L. from the Gulf of Gdansk (Baltic Sea, Poland). A simple method, based on an osmotic shock, was used to permeabilise gill cells. The cytometric pattern of normal clams consisted of 2 peaks, a major peak B and a smaller peak C. The cytometric pattern of affected clams consisted of 2 peaks named B' and C'. Two parameters were used to define the stages of abnormalities in M. balthica clams based on the percentage of cells in peaks B, C, B' and C' and on the ratio between the fluorescence value of peaks B, C, B' and C' in all individuals. Three stages of neoplasia were clearly distinguished by flow cytometry considering peak C'. Stage 1 was characterised by a major... |
| Tipo: Text |
Palavras-chave: Baltic Sea; Macoma balthica; Neoplasia; Cytogenetics; Histology; Flow cytometry. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2918.pdf |
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| Sokolowski, A; Wolowicz, M; Hummel, H; Bogaards, R. |
| Physiological and behavioral responses to Cu exposure were measured in the Baltic clam Macoma balthica from the Gulf of Gdansk, southern Baltic Sea. The burrowing activity, mortality rate, glycogen content, condition index and free amino acid (FAA) composition were analysed as indicators of stress in a series of field and laboratory studies. M. balthica exposed to Cu showed clear Cu-concentration related differences in burrowing activity and mortality rate, but no consistent differences in the condition index, glycogen content, and free amino acids. The clams from a less polluted area reacted more strongly and were more sensitive to additional stress as compared to organisms from a more polluted region. The effect of Cu on the ecophysiology of Baltic clams... |
| Tipo: Text |
Palavras-chave: Stress; Cuivre; Écophysiologie; Macoma balthica; Mer Baltique; Stress; Copper; Ecophysiology; Macoma balthica; Baltic Sea. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00325/43606/43925.pdf |
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| Smolarz, Kasia; Renault, Tristan; Soletchnik, Patrick; Wolowicz, Macciek. |
| Using flow cytometry, 234 Macoma balthica were examined during a survey to determine frequency of neoplasia in the Gulf of Gdansk (Poland). Clams were collected in 4 locations and DNA content in gill tissue cells was determined by flow cytometry using propidium iodide staining. Cell permeabilization was induced by osmotic shock. Prevalence of neoplasia ranged from 9.6 to 26.7 % depending on location. DNA content in aneuploid cells was higher than in normal dividing cells. The fluorescence value for aneuploid cells corresponded to tetraploid/pentaploid cells. Three stages of neoplasia were defined, based on the percentage of aneuploid cells determined by flow cytometry. Histopathological and cytogenetic analyses were also carried out on the same clams for... |
| Tipo: Text |
Palavras-chave: Prevalence; Macoma balthica; Flow cytometry; Gill neoplasia. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2919.pdf |
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| Smolarz, Kasia; Renault, Tristan; Wolowicz, Maciej. |
| In the Baltic clam Macoma balthica from the Gulf of Gdansk, neoplasia is considered as a serious epizootic linked to bad environmental conditions and high levels of pollutants. Previous research was focused on the diagnosis, prevalence, seasonality and histopathological characteristic of the cancer. This study is focused on electron microscopy analyses describing cell ultrastructure abnormalities related to neoplasia. Examinations using the electron microscopy highlighted changes confined to anatomic ultrastructures, shapes and functions of neoplastic cells. The lobulated appearance of the nucleus, changes in cellular matrix and the occurrence of large granular cells with hyperchromatic nuclei, atypical Golgi structures and deterioration of rough... |
| Tipo: Text |
Palavras-chave: Gulf of Gdansk; Macoma balthica; Neoplasia; Myofibroblast like cells; Cell ultrastructures. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1689.pdf |
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