SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

BLECHA, I. M. Z.; SOUSA, I. I.; FERREIRA, A. B. R.; FEIJO, G. L. D.; TORRES JUNIOR, R. A. de A.; SIQUEIRA, F..

The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST gene, new primers were designed, and for the SNP of the CAPN1 gene, the same primers previously described in the literature were used. Bonsmara, Caracu, Senepol, Nelore, and Angus bulls were chosen from among the most used bulls in breeding programs according to their genealogy and the lowest possible degree of parentage between them to ensure an...

Association of polymorphisms in the leptin and thyroglobulin genes with meat quality and carcass traits in beef cattle

Palavras-chave: Animal breeding; Marker-assisted selection; Meat tenderness.

Ano: 2019

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117678

R. Bras. Zootec.

Carvalho,Thiago Dutra de; Siqueira,Fabiane; Torres Júnior,Roberto Augusto de Almeida; Medeiros,Sérgio Raposo de; Feijó,Gelson Luís Dias; Souza Junior,Maury Dorta de; Blecha,Isabella Maiumi Zaidan; Soares,Cleber Oliveira.

The objective of the present study was to estimate the allelic and genotypic frequencies of the polymorphisms E2FB (AY138588.1: c.305C> T), located in the leptin gene (LEP), and TG5 (X05380.1:g.-422C>T), located in the thyroglobulin gene (TG), and evaluate the association of these polymorphisms in crossbred cattle of seven distinct genetic groups with the following traits: slaughter weight (SW), hot carcass weight (HCW), hot carcass yield (HCY), carcass fat thickness (CFT), ribeye area (REA), marbling (MARM) and shear force (SF). The animals were genotyped using the PCR-RFLP (Polymorphism Chain Reaction-Restriction Fragment Length Polymorphism) technique, using 201 products obtained from F1 Caracu × Nellore, Angus × Nellore and Valdostana × Nellore...

Beef tenderness of Nellore cattle finished with supplemented yeast and probiotic in the north of Mato Grosso.

Palavras-chave: Fat thickness; Marker-assisted selection; Meat tenderness; Shear force; SNP.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000004

NUNES, P. C. de A.; KAMCHEN, S. G.; ROCHA, B. F. da; CARLOS, F. de M.; XAVIER, I. M.; POLIZEL NETO, A.; PAREIRA, D. H.; PEDREIRA, B. C. e.

Important world markets are requiring safer, consumer-friendly food, encouraging further studies on beef information. Feed supplementation of grazing cattle may influence on the beef tenderness, mainly associated with the animal growth and fat cover degree. The determination of the physicochemical composition of the carcass becomes fundamental within this context, since it makes possible to evaluate the effect of any type of treatment to which the animals have been submitted. Aimed, with study, evaluate the beef tenderness of Nellore cattle supplemented with different additives. Twenty-eight non-castrated males of the Nellore breed were randomly divided into four supplementation groups (Group 1 = Urea; Group 2 = Urea + Optygen; Group 3 = Group 2 + Yeasts;...

Bovine μ-calpain (CAPN1) gene polymorphisms in Brangus and Brahman bulls

Palavras-chave: Beef; Beef cattle; Beef carcasses; Shear stress; Meat tenderness.

Ano: 2017

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1086385

JBAG

Soria,LA; Corva,PM; Huguet,MJ; Miño,S; Miquel,MC.

The bovine CAPN1 gene encodes the large subunit of μ-calpain, which is thought to be one of the most important enzymes involved in postmortem beef tenderization. Three SNPs in CAPN1 (SNP 316, SNP 530 and SNP 4751) have been associated with beef tenderness in different beef cattle breeds. The objective of this work was to implement genotyping strategies for CAPN1 markers as part of a project pursuing the identifi cation and validation of molecular markers associated with bovine meat quality and composition. Three PCR-RFLP methods were designed to determine genotypes of 64 bulls (11 Angus, 43 Brangus and 10 Brahman). Unexpected patterns resulting from the PCR-RFLP analysis at SNP 316 and SNP 530 were resolved by cloning and sequencing and lead to...

URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332010000100007

Palavras-chave: Beef cattle; Meat tenderness; U-calpain; Molecular markers; Single nucleotide polymorphism.

Ano: 2010

Candidate SNPs for carcass and meat traits in Nelore animals and in their crosses with Bos taurus

PAB

Curi,Rogério Abdallah; Krauskopf,Monique Marcondes; Hadlich,Janaína Conte; Fortes,Marina Rufino Salinas; Vankan,Dianne Margaret; Silva,Josineudson Augusto II Vasconcelos; Oliveira,Henrique Nunes de; Mota,Marcílio Dias Silveira da.

The objective of this work was to evaluate the effects of single-nucleotide polymorphisms (SNPs) in the genes IGF1 (AF_017143.1:g.198C>T), MSTN (AF_320998.1:g.433C>A), MYOD1 (NC_007313:g.1274A>G) and MYF5 (NC_007303:g.1911A>G) on carcass and meat traits in Nelore (Bos indicus) and Nelore x B. taurus. A total of 300 animals were genotyped and phenotyped for rib eye area (REA), backfat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The effects of allele substitution for each SNP were estimated by regression of the evaluated phenotypes on the number of copies of a particular allele using the general linear model. The polymorphism at IGF1 was non-informative in Nelore animals. In crossbred...

Comparison of proximate chemical composition and texture of cupim, Rhomboideus m. and lombo, Longissimus dorsi m. of Nelore (Bos indicus)

Palavras-chave: Bos indicus; Candidate gene; Fat deposition; Meat tenderness; Polymorphism.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000200019

BABT

Pedrão,Mayka Reghiany; Lassance,Fernanda; Souza,Nilson Evelazio de; Matsushita,Makoto; Telles,Paulo; Shimokomaki,Massami.

The proximate chemical composition of hump, known in Brazil as cupim, Rhomboideus m. (RB), of Nelore (Bos indicus) aged 24 months revealed it to be a unique beef muscle. It presents a lipid fraction 14-fold as high as that of Longissimus dorsi m. (LD) taken from the same animal (p<0.05), the highest value reported so far. This was unequivocally observed by the histological evaluation. Proportionally more protein fraction and conversely less moisture were also observed in RB. Analysis of collagen and its crosslinking with hydroxylysylpyridinium (HP) showed there to be 22.9% more collagen and 14-fold as much HP in RB as in LD. Contrary to the expectations, the tenderness of fresh samples evaluated by Warner Bratzler shear force measurements led to values...

Feed efficiency indexes and their relationships with carcass, non-carcass and meat quality traits in Nellore steers.

Palavras-chave: Zebu breed; Collagen; Hydroxylysylpyridinium; Meat tenderness; Marbling fat.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300023

NASCIMENTO, M. L.; SOUZA, A. R. D. L.; CHAVES, A. S.; CESAR, A. S. M.; TULLIO, R. R.; MEDEIROS, S. R. de; MOURÃO, G. B.; ROSA, A. do N.; FEIJO, G. L. D.; ALENCAR, M. M. de; LANNA, D. P. D..

Five hundred and seventy-five Nellore steerswere evaluated for residual feed intake and residual feed intake and gain and their relationships between carcass, non-carcass and meat quality traits. RFI was measured by the difference between observed and predicted dry matter intake and RIG was obtained by the sum of ?1*RFI and residual gain. Efficient and inefficient animals were classified adopting ±0.5 standard deviations from RFI and RIG mean. A mixed model was used including RFI or RIG and contemporary group as fixed effects, initial age as covariate and sire and experimental period as random effects, testing the significance of the regression slope for each evaluated trait. RIG was positively related to longissimus muscle area. Efficient-RFI animals had...

Genetic polymorphisms related to meat traits in purebred and crossbred Nelore cattle.

Palavras-chave: Internal fat; Visceral organs; Bos indicus; Gado zebu; Beef cattle; Zebu; Feed conversion; Meat tenderness.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/handle/doc/1066238

CURI, R. A.; FORTES, M. R. S.; CHARDULO, L. A. L.; SILVEIRA, A. C.; DE BENI ARRIGONE, M.; MARTINS, C. L.; ASSUMPÇÃO, M. E. O. d' A.; OLIVEIRA, H. N. de.

Abstract - The objective of this work was to estimate the allelic and genotypic frequencies of CAST/XmnI, a calpastatin gene polymorphism, and CAPN530, a calpain 1 large subunit gene polymorphism, in different beef genetic groups (Nelore and Nelore x Bos taurus), and to investigate associations between these polymorphisms and carcass and meat traits. Three hundred animals ? comprising 114 Nelore, 67 Angus x Nelore, 44 Rubia Gallega x Nelore, 41 Canchim, 19 Brangus three-way cross and 15 Braunvieh three-way cross ? were genotyped by PCR-RFLP and phenotyped for rib-eye area (REA), back-fat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The occurrence of the two alleles of the CAST/XmnI and CAPN530 single...

Genetic polymorphisms related to meat traits in purebred and crossbred Nelore cattle

Palavras-chave: Calpain gene; Calpastatin gene; Single nucleotide polymorphisms; Bos indicus; Meat tenderness.

Ano: 2009

URL: http://www.alice.cnptia.embrapa.br/handle/doc/662457

PAB

Curi,Rogério Abdallah; Fortes,Marina Rufino Salinas; Chardulo,Luis Artur Loyola; Silveira,Antonio Carlos; Arrigoni,Mário De Beni; Martins,Cyntia Ludovico; Assumpção,Mayra Elena Ortiz D' Avila; Oliveira,Henrique Nunes de.

The objective of this work was to estimate the allelic and genotypic frequencies of CAST/XmnI, a calpastatin gene polymorphism, and CAPN530, a calpain 1 large subunit gene polymorphism, in different beef genetic groups (Nelore and Nelore x Bos taurus), and to investigate associations between these polymorphisms and carcass and meat traits. Three hundred animals - comprising 114 Nelore, 67 Angus x Nelore, 44 Rubia Gallega x Nelore, 41 Canchim, 19 Brangus three-way cross and 15 Braunvieh three-way cross- were genotyped by PCR-RFLP and phenotyped for rib-eye area (REA), back-fat thickness (BT), intramuscular fat (IF), shear force (SF) and myofibrillar fragmentation index (MFI). The occurrence of the two alleles of the CAST/XmnI and CAPN530 single nucleotide...

Genome-wide detection of CNVs and their association with meat tenderness in Nelore cattle.

Palavras-chave: Bos indicus; Calpain gene; Calpastatin gene; Meat tenderness; Single nucleotide polymorphisms.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001200015

SILVA, V. H. da; REGITANO, L. C. de A.; GEISTLINGER, L.; PÉRTILLE, F.; GIACHETTO, P. F.; BRASSALOTI, R. A.; MOROSINI, N. S.; ZIMMER, R.; COUTINHO, L. L..

Brazil is one of the largest beef producers and exporters in the world with the Nelore breed representing the vast majority of Brazilian cattle (Bos taurus indicus). Despite the great adaptability of the Nelore breed to tropical climate, meat tenderness (MT) remains to be improved. Several factors including genetic composition can influence MT. In this article, we report a genome-wide analysis of copy number variation (CNV) inferred from Illumina1 High Density SNP-chip data for a Nelore population of 723 males. We detected >2,600 CNV regions (CNVRs) representing 6.5% of the genome. Comparing our results with previous studies revealed an overlap in 1400 CNVRs (>50%). A total of 1,155 CNVRs (43.6%) overlapped 2,750 genes. They were enriched for...

Heat shock proteins on beef quality

Palavras-chave: Genômica; Maciez da carne; CNV; Nelore; Gado Nelore; Meat tenderness.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1055112

PAB

Moncau,Cristina Tschorny; Rosa,Alessandra Fernandes; Eler,Joanir Pereira; Balieiro,Júlio César de Carvalho.

Abstract: The objective of this work was to quantify heat shock proteins (HSP) 27 and 70 in the Longissimus dorsi muscle of cattle during aging and to check their potential as biomarkers for meat quality. A total of 191 steers ½ South African Simmental x ½ Nellore (16-18 months, 391.7±99.7 kg), castrated, and feedlot finished were used. Meat quality was measured by pH, color, cooking loss, and shear force (SF) at 1 and 14 days of aging time. HSP27 and HSP70 were quantified according to the SF values in the more and less tender meat groups, with 20 samples each, for each aging time. HSP27 concentrations in more and less tender meat decrease from 1 to 14 days of aging, and do not differ when evaluated at the same period. HSP70 concentrations in more tender...

Meat quality of Santa Inês sheep raised in confinement with diet containing cactus pear replacing corn

Palavras-chave: Bos taurus indicus; Biomarker; HSP27; HSP70; Meat tenderness.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100090

R. Bras. Zootec.

Costa,Roberto Germano; Pinto,Tiago Ferreira; Medeiros,Geovergue Rodrigues de; Medeiros,Ariosvaldo Nunes de; Queiroga,Rita de Cássia Ramos do Egypto; Treviño,Israel Hernandez.

The objective of this study was to assess the tissue component yields and the physical, chemical and sensory characteristics of meat from Santa Inês sheep fed diets in which cactus pear partially or completely replaced corn. The study used 45 Santa Inês rams with initial live weight of 25±2.5 kg and final weight of 35±1.5 kg in a completely randomized design with five treatments (0, 25, 50, 75 and 100%) and nine replicates per treatment. The leg of the animal was used to analyze the tissue component yields, and the longissimus dorsi muscle was used for assessment of the sensory characteristics and the physical and chemical compositions. The inclusion of cactus pear in the diet increased the adipose tissue percentage quadratically, up to 50%, reducing the...

Quality characteristics of Longissimus dorsi muscle from Bos indicus animals treated with vitamin D3

Palavras-chave: Meat tenderness; Opuntia ficus indica; Proximate composition; Sensory characteristics.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000200028

Scientia Agricola

Pedreira,Aparecida Carla de Moura Silveira; Luchiari Filho,Albino; Leite,Vanderley Benedito de Oliveira; Carvalho,Marina Hojaij.

Among several techniques to improve beef tenderness, vitamin D3, important for calcium mobilization, has recently been developed as an alternative. It acts on the intracellular calcium-dependent proteases (mu- and m-calpain). Ten days prior to slaughter, 36 Nelore steers were fed 0, 3, 6 and 9 million IU of supplemental vitamin D3 (D3) per animal per day (an-1 d-1). Animals were slaughtered and tenderness (shear force), cooking losses (1, 8 and 15 days of aging), sensory evaluation, and minerals in blood plasma and muscle (Longissimus dorsi) were measured. There were no differences (P > 0.05) among treatments for blood plasma and muscle mineral concentration, evaporation losses, and sensory juiciness. For drip and total loss, the smallest losses were...

Screening for Single Nucleotide polymorphisms in MYOD1 gene and its association with meat tenderness in cattle.

Palavras-chave: Nelore cattle; Meat tenderness; Carcass characteristics.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400005

TIZIOTO, P. C.; MEIRELLES, S. L.; SOUZA, M. M. de; OLIVEIRA, P. S. N. de; SIQUEIRA, F.; ROSA, A. do N.; TULLIO, R. R..

2011

Study of Polled Nellore lineages for carcass characteristic and meat quality.

Palavras-chave: Nucleotide polymorphisms; MYOD1 gene; Meat tenderness; Beef cattle.

Ano: 2011

URL: http://www.alice.cnptia.embrapa.br/handle/doc/908938

MAGNABOSCO, C. de U.; MOREIRA, L. da C.; LOPES, F. B.; REGITANO, L. C. de A.; TULLIO, R. R.; NASSU, R. T.; CASTRO, L. M. de; SAINZ, R. D..

2014

Valuing Fed Cattle Using Objective Tenderness Measures

Palavras-chave: Ancestry; Carcass; Quality; Meat tenderness; Selection; Race Zebu.

Ano: 2014

URL: http://www.alice.cnptia.embrapa.br/handle/doc/992598

AgEcon

Riley, John Michael; Schroeder, Ted C.; Wheeler, Tommy L.; Shackelford, Stephen D.; Koohmaraie, Mohammad.

Beef tenderness is critical in consumer satisfaction with beef steak products. Current fed cattle valuation systems do not differentiate carcasses based upon tenderness variation. However, considerable research indicates consumers are willing to pay more for tender relative to tough beef steak. This article develops a tenderness-augmentation to current fed cattle grid pricing systems. Using a large set of actual carcasses, we determine that a tenderness-augmented price grid would reorder fed cattle value by on average nearly $5.00/cwt dressed relative to current valuation methods. Substantial opportunity is present to improve beef tenderness through new price signals to producers.

Tipo: Journal Article

Palavras-chave: Beef quality; Meat tenderness; Cattle value; Cattle price; Agribusiness; Farm Management; Food Consumption/Nutrition/Food Safety; Livestock Production/Industries; Q11; Q13; M31.

Ano: 2009

URL: http://purl.umn.edu/48747

VIS-NIRS to predict meat quality is anatomical point specific.

BONIN, M. de N.; SILVA, S. da L. e; GOMES, R. da C.; MENEZES, G. R. de O.; SURITA, L. M. A.; FAVERO, R.; FERRAZ, A. L. J.; FEIJO, G. L. D..

The Vis-NIRS is a non-destructive, simple, rapid, and safe method for evaluating meat quality. Studies using VIS/NIRS to predict the meat quality are based mainly in information collected on longissimus thoracis (LT), at 12th rib, which differs in quality from LT at 5th rib. Accordingly, the present study aimed to create VIS/NIRS calibrations models for LT at 5th (LT5) and 12th rib (LT12) and test their accuracy to predict meat quality of beef samples. A total of 1,508 steer and heifer carcasses, averaging 20 months (± 4.00) were used in this study.