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Registros recuperados: 7
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Antimicrobial susceptibility profile and research of mec A and erm genes in coagulase-negative staphylococci isolated from platelet concentrates bags BJPS
Martini,Rosiéli; Hörner,Rosmari; Graichen,Daniel Ângelo Sganzerla.
Abstract In recent years, several studies have described the clinical impact of bacterial infection associated with transfusion of platelet concentrates (PCs). Among the blood components, PCs are responsible for the highest rates of bacterial contamination as well as septic transfusion reactions. We assessed antimicrobial susceptibility profile, resistance to methicillin (MRCoNS), and resistance to macrolides, lincosamides and streptogramins of group B (MLSB) of 16 coagulase-negative staphylococci (CoNS) isolates from an investigation in 691 PCs bags. We then compared conventional and automated phenotypic methods, disc diffusion test (DD) and VITEK(r) 2, respectively as well as phenotypic and genotypic methods (Polymerase Chain Reaction - PCR). All CoNS...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial resistance; Staphylococcus sp; Platelet concentrates; MecA; Erm..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100609
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Description of Methicillin-resistant Staphylococcus pseudintermedius from canine pyoderma in Minas Gerais state, Brazil Arq. Bras. Med. Vet. Zootec.
Bourguignon,E.; Viçosa,G.N.; Corsini,C.M.M.; Moreira,M.A.S.; Nero,L.A.; Conceição,L.G..
Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is of worldwide concern in veterinary medicine. The identification of resistant strains is necessary for proper treatment and the prevention of its propagation among animals. This study aimed to identify S. pseudintermedius isolated from canine pyoderma and evaluate their resistance profiles. Lesions from 25 dogs with pyoderma were sampled. Bacterial isolates were subjected to phenotypic and genotypic analysis for identification of the causative agent. S. pseudintermedius isolates were subjected to SmaI macrorestriction analysis and PFGE for genetic grouping, and PCR to identify the presence of the mecA gene. Their resistance profiles against 12 antimicrobials were also assessed. According to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus pseudintermedius; Canine pyoderma; MecA; PFGE; Antimicrobial resistance.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200299
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Detection of methicillin resistance in Staphylococcus aureus isolated from pediatric patients: is the cefoxitin disk diffusion test accurate enough? BJID
Mimica,M.J.; Berezin,E.N.; Carvalho,R.L.B.; Mimica,I.M.; Mimica,L.M.J.; Sáfadi,M.A.P.; Schneider,E.; Caiaffa-Filho,H.H..
We evaluated the performance of several methods for the detection of methicillin resistance in Staphylococcus aureus using 101 clinical S. aureus isolates from pediatric patients in a tertiary hospital in Brazil; 50 isolates were mecA-positive and 51 were mecA-negative. The Etest and oxacillin agar screening plates were 100% sensitive and specific for mecA presence. Oxacillin and cefoxitin disks gave sensitivities of 96 and 92%, respectively, and 98% specificity. Alterations of CLSI cefoxitin breakpoints increased sensitivity to 98%, without decreasing specificity. Our results highlight the importance of a continuing evaluation of the recommended microbiological methods by different laboratories and in different settings. If necessary, laboratories should...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Disc diffusion; Cefoxitin; MecA.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000400009
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Estafilococos resistentes à oxacilina isolados em casos de mastite subclínica em ovinos. Repositório Alice
ZAFALON, L. F.; VERISSIMO, C. J.; MAMIZUKA, E. M.; MARTINS, K. B.; ALMEIDA, L. M.; VESCHI, J. L. A..
Bactérias do gênero Staphylococcus estão entre os principais agentes causadores da mastite ovina. Um dos maiores entraves ao tratamento dos animais doentes são cepas resistentes aos antimicrobianos empregados. A pesquisa do gene mecA nos estafilococos é um instrumento auxiliar para a determinação de aspectos epidemiológicos da doença. Este trabalho teve por objetivo investigar a resistência à oxacilina em estafilococos coagulase-negativos isolados no leite de ovelhas com mastite subclínica. Foram analisadas 448 amostras de leite de dois rebanhos. Os micro-organismos isolados foram submetidos previamente a testes de sensibilidade a antibióticos in vitro a partir da técnica de difusão em disco. Naqueles resistentes à oxacilina nestes testes efetuou-se a...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Staphylococcus spp; Leite; Gene; MecA; Resistência.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/931684
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Genetic analysis of mecA gene and detection of homologue pbpD in Stahylococcus sciuri group BJM
Calazans-Silva,Amanda C.; Medeiros,Pedro T.C.; Araujo,Dayanne M.; Carvalho,Bruno O.; Coelho,Irene S.; Coelho,Shana M.O.; Souza,Miliane M.S..
Oxacillin/methicillin-resistance is related to the mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown, although evidences support that it is related to CNS, once mecA and a homologue gene, pbpD, were both detected in Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal swabs from equines of Army Biologic Institute, Rio de Janeiro. Pheno- and genotypic characterization provided 59.52% (25/42) and 78.57% (11/14) S. lentus and S. sciuri, respectively. It was observed that although all S. sciuri isolates tested positive for pbpD, there was no correlation with oxacillin-resistance. On the other hand, isolates tested positive for mecA gene also presented phenotypic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus sciuri; MecA; PbpD; Antimicrobial resistance.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200038
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Genotypic and phenotypic characterization of antimicrobial resistance in Staphylococcus Spp. isolated from cows after alternative treatment of bovine mastitis. Repositório Alice
FERREIRA, E. M.; ROMERO, L. C.; SILVA, L. P.; PEREIRA, L. E. C.; MALAGO JUNIOR, W.; CHAPAVAL, L.; CUNHA, M. de L. R. de S. da; ZAFALON, L. F..
Staphylococcus spp. is among the major microorganisms involved in the etiology of bovine mastitis, with the ability to quickly acquire antimicrobial resistance genes.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Homeopathy; SCCmeC; S epidermidis; MecA; BlaZ; Staphylococcus.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1116609
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Species identification, slime production and oxacillin susceptibility in coagulase-negative staphylococci isolated from nosocomial specimens BJM
Alcaráz,Lucía E.; Satorres,Sara E.; Lucero,Rodolfo M.; Centorbi,Olga N. Puig de.
Ninety-two coagulase negative staphylococci (CNS) (forty-five of clinical origin and forty-seven of environmental origin), collected in a hospital in San Luis, Argentina, from March to June, 1999, were identified to species level by the ID 32 Staph and API Staph System (bioMérieux). Slime production was investigated by the quantitative and qualitative methods. Oxacillin susceptibility was determined by the disk diffusion test (1 µg), the agar dilution method (0.125 to 4 mg/ml) and agar screen (6 µg/ml). The presence of mecA gene was investigated by PCR. The clinical CNS species most commonly isolated were S. epidermidis, S. haemolyticus, S. hominis and S. saprophyticus. The frequency of slime production by clinical and environmental isolates was similar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coagulase-negative staphylococci; Slime; MecA; Oxacillin-R.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100010
Registros recuperados: 7
Primeira ... 1 ... Última
 

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