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| Registros recuperados: 11 | |
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| Wei,Shiping; Hurley,James; Jiang,Zhenglong; Wang,Siwen; Wang,Yuanyuan. |
| The use of the filamentous fungus, Ashbya gossypii, to improve riboflavin production at an industrial scale is described in this paper. A riboflavin overproducing strain was isolated by ultraviolet irradiation. Ten minutes after spore suspensions of A. gossypii were irradiated by ultraviolet light, a survival rate of 5.5% spores was observed, with 10% of the surviving spores giving rise to riboflavin-overproducing mutants. At this time point, a stable mutant of the wild strain was isolated. Riboflavin production of the mutant was two fold higher than that of the wild strain in flask culture. When the mutant was growing on the optimized medium, maximum riboflavin production could reach 6.38 g/l. It has even greater promise to increase its riboflavin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Riboflavin; Ashbya gossypii; Ultraviolet irradiation; Medium optimization. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200003 |
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| Hao,Zhikui; Cai,Yujie; Liao,Xiangru; Zhang,Xiaoli; Fang,Zhiyou; Zhang,Dabing. |
| The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chitinase; Medium optimization; Response surface method; Inulin; Chitinolyticbacter meiyuanensis SYBC-H1. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100019 |
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| Chaichanan,Jirapan; Wiyakrutta,Suthep; Pongtharangkul,Thunyarat; Isarangkul,Duangnate; Meevootisom,Vithaya. |
| To optimize the medium for high zofimarin production, sucrose maltose, glucose, tryptone and peptone were used in an orthogonal array design experiment, where the highest value of zofimarin produced was 25.6 µg/mL. This value was about 3 times higher than that obtained with Czapek yeast extract (CzYE) culture medium. A study with Plackett-Burman design showed that sucrose, maltose, glucose and NaNO3 were significant factors in zofimarin production. Further studies using central composite design (CCD) showed the significance of glucose and the interactions of these critical components affecting zofimarin production. Multiple regression analysis of the data yielded a poor fit as shown by the mismatch of the model with these variable factors. When a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zofimarin; Secondary metabolite; Medium optimization; Statistical methods; Endophytic fungus. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100042 |
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| Zárate-Chaves,Carlos Andrés; Romero-Rodríguez,María Camila; Niño-Arias,Fabián Camilo; Robles-Camargo,Jorge; Linares-Linares,Melva; Rodríguez-Bocanegra,María Ximena; Gutiérrez-Rojas,Ivonne. |
| The present work was aimed at optimizing a culture medium for biomass production and phenolic compounds by using Ganoderma lucidum. The culture was optimized in two stages; a Plackett-Burman design was used in the first one for identifying key components in the medium and a central composite design was used in the second one for optimizing their concentration. Both responses (biomass and phenolic compounds) were simultaneously optimized by the latter methodology regarding desirability, and the optimal concentrations obtained were 50.00 g/L sucrose, 13.29 g/L yeast extract and 2.99 g/L olive oil. Maximum biomass production identified in these optimal conditions was 9.5 g/L and that for phenolic compounds was 0.0452 g/L, this being 100% better than that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Medium optimization; Ganoderma lucidum; Plackett-Burman; Central composite design; Phenolic compounds. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100032 |
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| Damaso,Mônica Caramez Triches; Andrade,Carolina M.M. Carvalho; Pereira Jr,Nei. |
| In recent years, xylanases have expanded their use in many processing industries, such as pulp and paper, food and textile. Thermomyces lanuginosus IOC-4145 was able to produce a very high level of cellulase-free xylanase in shaken cultures using corncob as substrate (500 U/mL). An optimization of the medium composition in submerged fermentation was carried out aiming at a low cost medium composition for enzyme production. Statistical experiment design was employed for this purpose, pointing out corncob as the most important parameter, which affects enzyme production. Additionally, the influence of several chemicals on xylanase activity was investigated in the crude extract. A slight stimulation of the enzyme (5-15%) was achieved with NaCl and urea, both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xylanases; Thermomyces lanuginosus; Corncob; Submerged fermentation; Medium optimization. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400011 |
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| Shakibaie,Mojtaba; Ameri,Atefeh; Ghazanfarian,Roya; Adeli-Sardou,Mahboubeh; Amirpour-Rostami,Sahar; Torkzadeh-Mahani,Masoud; Imani,Mehdi; Forootanfar,Hamid. |
| ABSTRACT The ability of four Aspergillus strains for biosynthesis of kojic acid was evaluated among which Aspergillus terreus represented the highest level (2.21 g/L) of kojic acid production. Improvement kojic acid production ability of A. terreus by random mutagenesis using different exposure time to ultraviolet light (5–40 min) was then performed to obtain a suitable mutant of kojic acid production (designated as C5-10, 7.63 g/L). Thereafter, design of experiment protocol was employed to find medium components (glucose, yeast extract, KH2PO4 (NH4)2SO4, and pH) influences on kojic acid production by the C5-10 mutant. A 25-1 fractional factorial design augmented to central composite design showed that glucose, yeast extract, and KH2PO4 were the most... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kojic acid; Aspergillus terreus; UV mutagenesis; Medium optimization. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400865 |
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| Registros recuperados: 11 | |
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