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A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton Biocell
Zou,Yi; Zhong,Wenping.
PH domains (pleckstrin homology) are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular apartments with assistances of alternative binding partners. PH domain-containing proteins are found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2, displayed moderate phosphoinositide binding specificity. Full length PEPP2 associated with both plasma membrane and microtubules. The membrane-associated PEPP2 nucleated at cell-cell contacts and the leading edge of migrating cells. Overexpression of PEPP2 increased membrane microviscosity,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microtubule; Membrane; Phosphoinositide; Microviscosity; Wortmannin.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300005
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Ameliorative Effects of Thymoquinone on Sperm Parameters and Testosterone Level of Nicotine-Treated Sprague Dawley Rats BABT
Rosli,Farah Dayana; Hashim,Noor Hashida; Osman,Khairul; Ibrahim,Siti Fatimah; Kabir,Nurul.
Abstract Thymoquinone (TQ), the main constituent of the volatile oil derived from Nigella sativa has shown pharmacological benefits against various diseases while nicotine is an active component in cigarette that is known to be detrimental. This study was conducted to assess the ameliorating effects of TQ on sperm count, membrane, mitochondria and testosterone of nicotine-treated rats. Rats were randomized into four groups: control, nicotine, TQ, and nicotine with TQ. Nicotine (5 mg/kg bwt/day) was subcutaneously injected for 30 days to induce damaging effects on sperm and testosterone level. Rats were force-fed with TQ (5 mg/kg bwt/day) for the following 30 days. Sperm count was reduced in the nicotine group (26.72 ± 1.64 106/mL) but showed a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thymoquinone; Nicotine; Rat sperm; Membrane; Mitochondria; Testosterone.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100304
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Avaliacao da disponibilidade de fosforo no solo para a cultura do milho. Repositório Alice
MIOLA, G. R.; TEDESCO, M. J.; BISSANI, C. A.; GIANELLO, C.; CAMARGO, F. A. de O..
Conduziu-se um experimento, em 1994, em casa de vegetacao com seis solos do Estado do Rio Grande do Sul, com objetivo de avaliar a disponibilidade de fosforo no solo extraido pelos metodos duplo acido (Mehlich-1), resinas em esferas (RE), em capsulas (RC) e em membrana (RM) e papeis de filtro impregnados com oxido de ferro em solo umedecido a 75% da capacidade de campo (D1) e solo saturado (D2). Os solos foram previamente incubados com quatro doses de fosforo e cultivados com milho durante 28 dias. Os coeficientes de determinacao obtidos entre o fosforo absorvido pelas plantas de milho e o fosforo extraido foram: 0,85 para o Mehlich-1; 0,82 para o RE; 0,84 para o RC; 0,89 para o RM; 0,75 para o D1 e 0,70 para o D2. As quantidades de fosforo extraidas pelos...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Resina; Membrana; Papeis de filtro; Metodos de analise do solo; Resin; Membrane; Filter paper; Methods of soil analysis.
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/handle/doc/86394
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Chill-induced changes in fatty acid composition of tonoplast vesicles from hypocotyls of Vigna unguiculata (L.) Walp. Braz. J. Plant Physiol.
Oliveira,Luciana M. N. de; Sobreira,Alana C. de M.; Monteiro,Fernando de P.; Melo,Dirce F. de.
The present study was undertaken to investigate the changes induced by chilling on fatty acid composition of tonoplast vesicles from hypocotyls of Vigna unguiculata (L.) Walp. The 7 day-old control seedlings were grown at 25°C while treated seedlings were submitted to low temperatures (10°C and 4°C) for 4 days after 3 days germination. The chilling stress resulted in a differential inhibition of plant growth at 10°C and 4°C. Following chilling at 10°C and 4°C, the rate of unsaturated to saturated fatty acids increased under chilling stress. Our results suggest that the increase of unsaturated fatty acids content as well as the higher rate unsaturated/saturate fatty acidsmight be used by V. unguiculata plants as an adaptation mechanism likely to maintain...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Chilling; Cowpea; Lipid; Fatty acids; Membrane; Vacuole.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000100008
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Effect of a mono-specific algal diet on immune functions in two bivalve species - Crassostrea gigas and Ruditapes philippinarum ArchiMer
Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois.
The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte...
Tipo: Text Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf
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Influence of depectinization in the ultrafiltration of West Indian cherry (Malpighia glabra L.) and pineapple (Ananas comosus (L.) Meer) juices Ciênc. Tecnol. Aliment.
Barrosi,Sueli T. D.; Mendes,Elisabete S; Peres,Leila.
West Indian cherry (Malpighia glabra L.) and pineapple (Ananas comosus (L.) Meer)juice clarification by cross-flow UF, using polysulphone hollow fiber and ceramic tubular membranes with, respectively, nominal molecular weight cut off values of 100kDaltons and average pore diameters of 0.01mm, were studied. The influence of enzymatic treatment using enzyme concentrations of 20, 100 and 300mg/L, a time of 90min and a temperature of 40ºC for depectinization was verified. The juices were then clarified in a laboratory scale filtration unit, with an effective filtration area of 0.12m² for the polysulphone hollow fiber membrane and of 0.005m² for the ceramic tubular membranes. The influence of enzymatic treatment on viscosity, turbidity and total pectin of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clarification; Enzyme treatment; Ceramic tubular; Polysulfone hollow fiber; Membrane.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612004000200006
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Knocking down chitin synthase 2 by RNAi is lethal to the cotton boll weevil. Repositório Alice
MACEDO, L. L. P.; SOUZA JUNIOR, J. D. D. de; COELHO, R. R.; FONSECA, F. C. A.; FIRMINO, A. A. P.; SILVA, M. C. M.; FRAGOSO, R. R.; ALBUQUERQUE, E. V. S.; SILVA, M. S.; ENGLER, J. de A.; TERRA, W. R.; GROSSI-DE-SA, M. F..
bitstream/item/168697/1/1-s2.0-S2452072117300096-main.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Peritrophic; Membrane; Insect pest control; RNAi (RNA interference); Midgut.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082142
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Leukocyte adhesion - a fundamental process in leukocyte physiology BJMBR
Gahmberg,C.G.; Valmu,L.; Tian,L.; Kotovuori,P.; Fagerholm,S.; Kotovuori,A.; Kantor,C.; Hilden,T..
Leukocyte adhesion is of pivotal functional importance. The adhesion involves several different adhesion molecules, the most important of which are the leukocyte ß2-integrins (CD11/CD18), the intercellular adhesion molecules, and the selectins. We and others have extensively studied the specificity and binding sites in the integrins and the intercellular adhesion molecules for their receptors and ligands. The integrins have to become activated to exert their functions but the possible mechanisms of activation remain poorly understood. Importantly, a few novel intercellular adhesion molecules have been recently described, which seem to function only in specific tissues. Furthermore, it is becoming increasingly apparent that changes in integrins and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leukocyte; Adhesion; Integrin; ICAM; Membrane; Glycoprotein.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500003
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MEND Syndrome: A case report with scanning electron microscopy findings of the collodion membrane Repositório Alice
CASTRO, L. A. S. de; ISAACSSON, H.; RAMPON, G.; HAS, C.; ALMEIDA JR, H..
2017
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Membrane.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1065815
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Phylogeny of canine coronavirus (CCoV) from Brazilian dogs based on membrane protein partial sequences Arq. Bras. Med. Vet. Zootec.
Guirao,M.P.; Souza,S.P.; Jerez,J.A.; Richtzenhain,L.J.; Brandão,P.E..
Este artigo descreve a anteriormente desconhecida diversidade molecular de amostras brasileiras de Coronavírus canino (CCoV). Vinte e duas amostras foram submetidas à análise da sequência parcial do gene codificador da proteína de membrana, sendo 12 classificadas como CCoV Tipo II e 10 como CCoV Tipo I e uma possível sublinhagem tipicamente brasileira foi encontrada para o CCoV Tipo II.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coronavirus; Dogs; Phylogeny; Membrane; Brazil.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000600042
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Pre-purification by membrane filtration of paralytic shellfish toxins from Alexandrium minutum dinoflagellate ArchiMer
Balti, Rafik; Brodu, Nicolas; Zhang, Jiaxuan; Amzil, Zouher; Drouin, Delphine; Sechet, Veronique; Masse, Anthony.
The Paralytic shellfish neurotoxins (PST) are of increasing interest for biomedical applications. The chemical synthesis is often complex and expensive that’s why the purification by membrane filtration of PST from Alexandrium minutum dinoflagellate was investigated. Disrupted micro-alga cells by ultrasonic treatment were diafiltred to let pass toxins through an ultrafiltration membrane. Then, the mean permeate was concentrated and diafiltrated by nanofiltration. Mean permeate fluxes equal to 187, 135 and 135 L.h–1.m-2 were obtained during the first diafiltration, the concentration step and the final diafiltration respectively. Up to 57 % (mol/mol) and 78 % (mol/mol) of organic matters and salts were removed respectively. Divalent ions were sparsely...
Tipo: Text Palavras-chave: Paralytic shellfish poisoning; Nanofiltration; Membrane; Alexandrium minutum; Purification.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00451/56286/57865.pdf
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Sanitary aspects and technological challenges of whole milk microfiltration at low temperatures PAB
Fagnani,Rafael; Mexia,Marisa Marroni; Puppio,Ana Amélia Nunes; Battaglini,Ana Paula Pavão.
Abstract: The objective of this work was to evaluate the effects of whole milk microfiltration at low temperatures on bacterial counts and on its shelf life. The microfiltration process was evaluated at two temperatures (30 and 50ºC) and compared with the slow pasteurization process. Both slow pasteurization and microfiltration reduced the initial counts of aerobic mesophilic and psychrotrophic bacteria, as well as total coliforms in whole milk. Microfiltration at 50ºC was as effective as the pasteurization process, since it reduced the initial count of aerobic mesophilic bacteria in 4.4 log cycles; increased the product's shelf life, which reached 30 days without exceeding 1,000 CFU mL-1; and eliminated coliform counts at the temperatures of 35 and 45ºC,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; Membrane; Microbiological quality; Pasteurization; Shelf life.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000800990
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Sustainable alternative for the food industry: converting whey and orange juice into a micro-filtered beverage Scientia Agricola
Fagnani,Rafael; Puppio,Ana Amélia Nunes; Zanon,Emely Osti.
ABSTRACT: Enhancing industrial sustainability by converting whey into alternative high value-added products is a scientific trend in food science and technology. However, without other ingredients, rennet, or sour, whey has an unappetizing flavor. This sensory challenge can be overcome by blending it with citrus flavor from orange juice. This study assessed a micro-filtered beverage from whey and orange juice without enzymatic treatment. Four formulations (27:10, 8.6:10, 2.4:10 and 1:10 v/v whey:juice ratio) were processed through a 1.4 μm microfiltration system with four different transmembrane pressures (1, 2, 3, and 4.15 bar) and then stored at 5 °C for 28 days. The micro-filtered beverage was analyzed for physicochemical, sensorial and microbial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Membrane; Dairy; Fruit; Fouling; Sensory evaluation.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000200136
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Tangential microfiltration of orange juice in bench pilot Ciênc. Tecnol. Aliment.
Venturini Filho,W. G.; Dornier,M.; Belleville,M. P..
The aim of this study was to introduce the tangential microfiltration (TMF) technique on the production of orange juice (TMFJ), and compare it with pasteurised juice (control) as regards chemical composition and sensorial characteristics. We used a TMF pilot equipped with four monotubular ceramic membranes (0.1, 0.2, 0.8 and 1.4mm) arranged in series with a filtering area of 0.005 m² each. Commercial flash-pasteurised orange juice was used as the initial product. Experiments were divided into three parts: a) the characterisation of the TMF pilot; b) optimisation of operational conditions; c) production of the TMFJ. In the second part, membrane with 0.8-mm pores presented best flux followed by those with 1.4-, 0.1-, and 0.2-mm pores. However, to guarantee...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beverage; Fruit; Sensorial analysis; Membrane.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612003000300006
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