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| Registros recuperados: 13 | |
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| Suarez,Carlos Alberto Galeano; Montano,Inti Doraci Cavalcanti; Nucci,Edson Romano; Iemma,Monica Rosas da Costa; Giordano,Raquel de Lima Camargo; Giordano,Roberto de Campos. |
| Bacillus megaterium is a promising host for expression of heterologous proteins. This paper reports the nutrient consumption patterns and production of metabolites for three different strains of B. megaterium, ATCC 14945, QMB 1551 and PV 361, which is QMB 1551 with seven constitutive plasmids deleted. 14 h cultivations in agitated flasks were run, for two different media: A (LB plus 10g/L glucose) and B (medium A, with the yeast extract replaced by tryptone). Strains PV361 and QMB 1551 showed higher maximum specific growth rates in medium B, reaching 0.42 h-1 and 0.48 h-1 respectively. The main by-products of the glucose overflow mechanism were acetate and lactate, for all three strains, which had preferential amino acids for substrate: Ala, Asp, Glu, Ser.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metabolic profile; Bacillus megaterium; Culture media. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400001 |
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| Prado,R.O.F.; Morales,B.J.E.; García,M.L.J.; Molina-Ochoa,J.; Valpuesta,S.G.; Hernández,R.J.A.; García,C.A.C.. |
| ABSTRACT English Thoroughbred horses, are widespread in Mexico and due to the lack of data on their exercise physiology, it is important to conduct exercise tests in order to obtain information the effects of exercise on more essential cations/anions in blood serum, as these horses are submitted to constant efforts. The study was carried out with 150 blood samples of English Thoroughbred horses clinically healthy. The blood sample collection was performed during three periods: 1) rest, 2) 30min after exercise (speed race of 12km/h for 30min with no rest) and 3) 60min after exercise. Mean values were calculated for cations (sodium and potassium) and anions (chloride and bicarbonate). The resulting data set was analyzed using Gaussian distribution and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: English thoroughbred horses; Blood chemistry; Metabolic profile; Osmotic balance. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300909 |
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| Gomes,G.L.G.C.; Carbonari,C.A.; Velini,E.D.; Trindade,M.L.B.; Silva,J.R.M.. |
| This study has aimed to develop a method for simultaneous extraction and determination by liquid chromatography and mass spectrometry (LC-MS/MS) of glyphosate, aminomethylphosphonic acid (AMPA), shikimic acid, quinic acid, phenylalanine, tyrosine and tryptophan. For the joint analysis of these compounds the best conditions of ionization in mass spectrometry and for chromatographic separation of the compounds were selected. Calibration curves and linearity ranges were also determined for each compound. Different extraction systems of the compounds were tested from plant tissues collected from sugarcane (Saccharum officinarum) and eucalyptus (Eucalyptus urophylla platiphylla) plants two days after the glyphosate application at the dose of 720 g a.e. ha-1.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Herbicide; LC-MS/MS; Metabolites; Metabolic profile. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000200295 |
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| Alves,Marilia; González,Félix; Carvalho,Nelcy; Mühlbach,Paulo; Lima,Verônica; Conceição,Tiago Reis; Wald,Vera. |
| Soybean by-products are currently used in Rio Grande do Sul, Brazil, as a source of protein in dairy cows. However, the high protein breakdown of this feed source in the rumen causes loss of the intrinsic quality of the protein and increases plasma urea with deleterious consequences to animal metabolism. Thermal treatment of soybean can overcome this limitation. The aim of this study was to evaluate the metabolism of dairy cows fed with thermally treated soybean and raw soybean, through the analyses of the metabolic profile of plasma and milk. Twelve Holstein cows in mid-lactation period were studied using four treatments: commercial concentrated of protein, soybean meal, raw soybean and roasted soybean. Protein and urea levels in milk were measured in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metabolic profile; Roasted soybean; Dairy cows; Milk; Protein; Urea. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000100037 |
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| Nogueira,Ériklis; Silva,Ailson Sebastião da; Amaral,Thaís Basso; Ítavo,Luís Carlos Vinhas; Dias,Alexandre Menezes; Mingoti,Gisele Zoccal. |
| To verify the effects of energy supplementation and fat on follicular dynamics, metabolic profile and the production of oocytes, 15 young heifers (Bos taurus indicus) with an average age of 14 months and with an initial weight of 256.35 kg were assigned to two treatments according to weight and follicular population as evaluated by ultrasonography: in T1 (1.0 × M), animals received 100% of the energy requirements for maintenance; in T2 (1.7 × M), animals received 170% of the energy requirements for maintenance, achieved by the addition of 200 g of Megalac®. After a period of adaptation to the diet, the treatments, blood collection and follicular aspirations were started using a randomized design. The dry matter intake and weight gain were lower in the 1.0... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metabolic profile; Nutrition and reproduction; OPU. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000900007 |
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| BRUNETTO, M. A.; RUBERTI, B.; HALFEN, D. P.; CARAGELASCO, D. S.; VENDRAMINI, T. H. A.; PEDRINELLI, V.; MACEDO, H. T.; JEREMIAS, J. T.; PONTIERI, C. F. F.; OCAMPOS, F. M. M.; COLNAGO, L. A.; KOGIKA, M. M.. |
| ABSTRACT: Chronic kidney disease (CKD) is highly prevalent in dogs, and metabolomics investigation has been recently introduced for a better understanding of the role of diet in CKD. This study aimed to compare the serum metabolomic profile of healthy dogs (CG) and dogs with CKD (CKD-T0 and CKD-T6) to evaluate whether the diet would affect metabolites. Six dogs (5 females; 1 male; 7.47 ± 2.31 years old) with CKD stage 3 or 4 (IRIS) were included. CG consisted of 10 healthy female dogs (5.89 ± 2.57 years old) fed a maintenance diet. Serum metabolites were analyzed by 1H nuclear magnetic resonance (1H NMR) spectra. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to assess differences in metabolomic... |
| Tipo: Artigo de periódico |
Palavras-chave: Renal dogs; Uremic toxins; Metabolic profile; Survival; Animal nutrition. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137959 |
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| Tabeleão, Vinícius Coitinho; UFPEL; Del Pino, Francisco Augusto Burcket; UFPEL; Goulart, Maikel Alan; UFPEL; Schwegler, Elizabeth; UFPEL; Moura, Sandra Vieira; UFPEL. |
| O objetivo deste estudo foi verificar a influência dos suplementos monensina e levedura sobre parâmetros ruminais e metabólicos de cordeiros semiconfinados. Foram utilizados 30 animais divididos em três grupos: controle (sem suplemento), ionóforo (500 g t-1 de Rumensin® ) e levedura (500 g t-1 de Beefsac® ). Os animais foram mantidos em sistema de semiconfinamento, recebendo ração concentrada equivalente a 2% do peso corporal. O fluido ruminal foi coletado a cada sete dias, a fim de determinar o pH, potencial redox, tempo de sedimentação e flutuação, cor, odor, consistência e movimentação de protozoários. O mesmo intervalo foi adotado para coleta de sangue, para determinação de glucose, ureia, colesterol total, triacilgliceróis,... |
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Palavras-chave: 5.04.03.00-1 Nutrição e Alimentação Animal probiótico; Ionóforo; Perfil metabólico; Perfil ruminal; Ovinos. probiotic; Oonophore; Metabolic profile; Ruminal profile; Ovine. |
| Ano: 2008 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4687 |
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| RODRIGUES NETO, J. C.; VIEIRA, L. R.; RIBEIRO, J. A. de A.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T.; ABDELNUR, P. V.. |
| Abstract: The expansion of the oil palm in marginal areas can face challenges, such as water deficit, leading to an impact on palm oil production. A better understanding of the biological consequences of abiotic stresses on this crop can result from joint metabolic profiling and multivariate analysis. Metabolic profiling of leaves was performed from control and stressed plants (7 and 14 days of stress). Samples were extracted and analyzed on a UHPLC-ESI-Q-TOF-HRMS system. Acquired data were processed using XCMS Online and MetaboAnalyst for multivariate and pathway activity analysis. Metabolism was affected by drought stress through clear segregation between control and stressed groups. More importantly, metabolism changed through time, gradually from 7 to... |
| Tipo: Artigo de periódico |
Palavras-chave: Metabolic profile; Análise multivariada; Estresse abiótico; Dendê; Elaeis Guineensis; Oil palm products; Abiotic stress; Multivariate analysis; Drought tolerance. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134385 |
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| Kawashima, Chiho; Sakaguchi, Minoru; Suzuki, Takahiro; Sasamoto, Yoshihiko; Takahashi, Yoshiyuki; Matsui, Motozumi; Miyamoto, Akio. |
| Metabolic hormones affect ovarian function in the cow. However, the relationship between metabolic factors and ovarian function is not clear in the postpartum primiparous cow because they are still growing. The aim of the present study was to investigate in detail the time-dependent profile of the metabolic hormones, metabolites, and milk yields of ovulatory and anovulatory primiparous cows during the first follicular wave postpartum. We used 16 primiparous Holstein cows and obtained blood samples for the profiles of metabolites (glucose; non-esterified fatty acid, NEFA; ketone body; total cholesterol; and aspartate aminotransferase), metabolic hormones (growth hormone, GH; insulin-like growth factor-I, IGF-1; and insulin), and progesterone every other day... |
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Palavras-chave: First follicular wave; Metabolic profile; Postpartum; Primiparous cow. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/921 |
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| García,C.A.C.; Prado,F.M.G.; Galicia,L.L.; Borderas,T.F.. |
| ABSTRACT Blood samples of 189 healthy Holstein cows classified as high-producers, low-producers, or dry cows, were collected with the purpose of establishing reference values for several biochemical analytes in Mexican dairy farms. Mean values were calculated for: 1) Energy profile: glucose, cholesterol, triglycerides, β-hydroxybutyrate, and non-esterified fatty acids, 2) Protein profile: urea, albumin, globulin, and total protein, 3) Mineral profile: calcium, phosphorus, sodium, potassium, magnesium, chlorine, carbon dioxide, bicarbonate, and anion gap, and 4) Hepatic enzyme: γ-glutamyl transpeptidase. The resulting data set was analyzed using Gaussian distribution and descriptive statistics. Confidence intervals of 95% were established. The linear... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dairy cow; Blood chemistry; Metabolic profile. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000200445 |
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| Registros recuperados: 13 | |
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