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Registros recuperados: 11
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Cloning and initial characterization of cellulolytic enzymes selected in a metagenomic approach. Repositório Alice
FAHEEM, M.; VIDAL, J. F. D.; FERNANDES, J. P. C.; OLIVEIRA. G. M.; CAROLINE, C. F.; SOUTO, B. de M.; PARACHIN, N. S.; QUIRINO, B. F.; BARBOSA, J. A. R. G..
2013
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Biofuel; Metagenome; Cellulose degradation; Enzymatic activity; Protein structure.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/961702
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Combined Whole-Cell High-Throughput Functional Screening for Identification of New Nicotinamidases/Pyrazinamidases in Metagenomic/Polygenomic Libraries ArchiMer
Zapata-perez, Ruben; Garcia-saura, Antonio G.; Jebbar, Mohamed; Golyshin, Peter N.; Sanchez-ferrer, Alvaro.
Nicotinamidases catalyze the hydrolysis of the amide bond in nicotinamide (NAM) to produce ammonia and nicotinic acid (NA). These enzymes are an essential component of the NAD(+) salvage pathway and are implicated in the viability of several pathogenic organisms. Its absence in humans makes them a promising drug target. In addition, although they are key analytical biocatalysts for screening modulators in relevant biomedical enzymes, such as sirtuins and poly-ADP-ribosyltransferases, no commercial sources are available. Surprisingly, the finding of an affordable source of nicotinamidase from metagenomic libraries is hindered by the absence of a suitable and fast screening method. In this manuscript, we describe the development of two new whole-cell methods...
Tipo: Text Palavras-chave: Amidohydrolases; Nicotinamidase; Functional screening; Metagenome; Library; High-throughput screening assays.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00362/47269/47232.pdf
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Composition and functionality of the wild and cultivated common bean rhizosphere microbiome. Repositório Alice
FLORES, S. W. S.; CHIARAMONTE, J. B.; ROSSMANN, M.; MENDES, R..
Abstract: Plants rely on their rhizosphere microbiome for specific functions, such as, nutrient acquisition and protection against diseases. The domestication and subsequent plant breeding neglected the important role of the rhizosphere microbiome on plant performance. Here, we tested the hypothesis that ancestor materials have higher ability to host beneficial microorganisms in the rhizosphere when compared to modern cultivars. For this, we assessed the composition and functionality of the rhizosphere microbiome associated with a wild (Wild Mex) and with a cultivated (IAC Alvorada) common bean grown in highly biodiverse soil (Amazonian Dark Earth). Antagonistic bacteria were isolated from common bean rhizosphere and total rhizosphere DNA was extracted for...
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Wild common bean; Metagenome; Rhizobacteria; Metagenomics; Microbial communities.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1036667
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Effect of nickel in the degradation of oil in soils contaminated with petroleum and nickel. Repositório Alice
TAKETANI, N. F.; TAKETANI, R. G.; LEITE, S. G. F.; MELO, I. S. de; LIMA-RIZZO, A. C.; ANDREOTE, F. D.; CUNHA, C. D. da.
Abstract: The pollution originated from the petrochemical industry is currently one of the main environmental concerns. Most of this is due to the high volume of toxic hydrocarbons that are produced and transported around the globe. However, this industry is also associated with toxic metals that are minor components of petroleum and used during refining processes. Here we have evaluated the impact of nickel in the biodegradation of crude oil in natural soils. We have assessed bacterial community profiles in these samples using ion torrent 16S rRNA gene sequencing and Real-Time PCR quantification and shotgun metagenome sequencing. We have found that the contamination with oil and nickel reduced the bacterial abundance compared with only soil. There was...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Metagenome; Metal contamination; Hydrocarbon remediation; Contaminação; Poluição do Solo; Petróleo; Níquel; Bactéria; Metagenomics; Soil pollution; Petroleum; Actinobacteria; Decontamination.
Ano: 2020 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125486
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Identification of clones with Beta-Glucosidase and cellobiohydrolase activities from a goat rumen metagenomic library. Repositório Alice
SOUTO, B. de M.; CARVALHO, L. S.; LOPIES, N. S. P. R.; CUNHA, I. S.; KRUGER, R. H.; QUIRINO, B. F..
2011
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Second generation bioethanol; Beta-Glucosidase; Cellobiohydrolase; Metagenome.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/908053
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Insights into multiple use water enterobacteria from the Brazilian semi-arid: the first approach by pyrosequencing Biological Sciences
Sodré Neto, Luiz; Regali-Seleghim, Mirna Helena; Araújo, Magnólia Fernandes Florêncio de; Blaha, Carlos Alfredo Galindo.
Thousands of people living in semi-arid regions face problems of drought and loss of water quality. In addition, high incidence of acute diarrheal diseases related to water consumption has been responsible for a high number of deaths and high economic costs for human health. Many of the diseases can be caused by the presence of enterobacteria in reservoirs that serve for multiple purposes. This study aimed to confirm the presence of potentially harmful bacteria, which was highlighted in other articles, and to reveal non-identified genera by culture-dependent methods and pyrosequencing. Twenty-three genera of the Enterobacteriaceae family were detected, with emphasis on Escherichia genus and confirmation of the presence of species such as Salmonella...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbiologia acute diarrheal diseases; Metagenome; Pyrosequencing..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/43447
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Molecular characterization of bacterial populations of different soils BJM
Pereira,Rodrigo Matheus; Silveira,Érico Leandro da; Scaquitto,Denilson César; Pedrinho,Eliamar Aparecida Nascimbém; Val-Moraes,Silvana Pompéia; Wickert,Ester; Carareto-Alves,Lúcia Maria; Lemos,Eliana Gertrudes de Macedo.
Until recently, few studies were carried out in Brazil about diversity of bacterial soil communities. Aiming to characterize the bacterial population in the soil through 16S rRNA analysis, two types of soil have been analyzed: one of them characterized by intensive use where tomato, beans and corn were cultivated (CS); the other analyzed soil was under forest (FS), unchanged by man; both located in Guaíra, São Paulo State, Brazil. Using specific primers, 16S rRNA genes from metagenomic DNA in both soils were amplified by PCR, amplicons were cloned and 139 clones from two libraries were partially sequenced. The use of 16S rRNA analysis allowed identification of several bacterial populations in the soil belonging to the following phyla: Acidobacteria,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metagenome; Microbial diversity; 16S rRNA.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400007
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Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach. Repositório Alice
SOUZA, R. C.; CANTAO, M. E.; NOGUEIRA, M. A.; VASCONCELOS, A. T. R.; HUNGRIA, M..
Abstract: The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Metagenome; Manejo do Solo; Plantio Direto; Microbiologia do Solo; Soil management; Soil enzymes; No-tillage; Metagenomics; Microbiome.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100838
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Outstanding impact of soil tillage on the abundance of soil hydrolases revealed by a metagenomic approach BJM
Souza,Renata Carolini; Cantão,Maurício Egídio; Nogueira,Marco Antonio; Vasconcelos,Ana Tereza Ribeiro; Hungria,Mariangela.
ABSTRACT The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metagenome; Microbiome; Soil enzymes; Soil management; No-tillage.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400723
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The rates and players of denitrification, dissimilatory nitrate reduction to ammonia (DNRA) and anaerobic ammonia oxidation (anammox) in mangrove soils Anais da ABC (AABC)
LUVIZOTTO,DANICE M.; ARAUJO,JULIANA E.; SILVA,MICHELE DE CÁSSIA P.; DIAS,ARMANDO C. F.; KRAFT,BEATE; TEGETMEYE,HALINA; STROUS,MARC; ANDREOTE,FERNANDO D..
Abstract Mangroves are ecosystems located in the transition zone between land and sea, characterized by periodic flooding that confer to its unique characteristics. Little is known about the transformation of nutrients that occur during the organic matter degradation in this system. In this study, we monitor the nitrogen transformations in soils from three mangroves with distinct levels of contamination using labeled 15NO3-. We also screened the mangroves metagenomes for the presence of genes that encode enzymes involved in denitrification (nirS, nirK, nosZ, norB and narG), anaerobic oxidation of ammonia (anammox) (hh, hao and hzo) and dissimilatory nitrate reduction to ammonium (DNRA) (nrfA). The transformations of 15NO3- indicated the balance of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: N cycling; Anaerobiosis; Metagenome; Environmental contamination.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000200503
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Tracking earthworm-microbe interactions and GHG emissions in green sugarcane production. Repositório Alice
BRAGA, L. P. P.; BROWN, G. G.; TSAI, S. M..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Ecologia microbial; Metagenoma; Microbial ecology; Metagenome; Agroecosystems; Matatranscriptomics.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1003407
Registros recuperados: 11
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