Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 10
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Construction and characterization of a metagenomic DNA library from the rhizosphere of wheat (Triticum aestivum) Phyton
Hernández-León,R; Martínez-Trujillo,M; Valencia-Cantero,E; Santoyo,G.
Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), including amidohydrolase, hydrolase, peptidase, serine protease, endonuclease and exonuclease. Another interesting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metagenomics; Rhizosphere; Wheat.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572012000100018
Imagem não selecionada

Imprime registro no formato completo
Functional Genes of Microorganisms, Comprehending the Dynamics of Agricultural Ecosystems BABT
Reali,Catiusca; Pilz Júnior,Harry Luiz; Meile,Jean Christophe; Galindo,Sabine Shorr; Fiuza,Lidia Mariana.
ABSTRACT The microbial composition of different types,in ecosystems (including agro-ecosystems), has been investigated in a rapidly growing number of studies in the past few years. The importance of microorganisms, regarding the maintenance and stability of nutrients in agroecosystems, is a key to maintain the sustainability of a crop. Molecular tools to study microbial communities are possible through many methods such as RISA, DGGE, TGGE, clone libraries, T-RFLP, RAPD, SSCP and more recently NGS (Next-Generation Sequencing). DGGE is widely employed to characterize the diversity and the community dynamics of microorganisms in the environment, making possible to find out specific groups through functional genes, allowing access to data that cannot be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agroecosystems; Functional genes; Bacteria; DGGE; Metagenomics; Microbial ecology.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100201
Imagem não selecionada

Imprime registro no formato completo
Genomic ecology of Marine Group II, the most common marine planktonic Archaea across the surface ocean ArchiMer
Pereira, Olivier; Hochart, Corentin; Auguet, Jean-christophe; Debroas, Didier; Galand, Pierre E..
Planktonic Archaea have been detected in all the world's oceans and are found from surface waters to the deep sea. The two most common Archaea phyla are Thaumarchaeota and Euryarchaeota. Euryarchaeota are generally more common in surface waters, but very little is known about their ecology and their potential metabolisms. In this study, we explore the genomic ecology of the Marine Group II (MGII), the main marine planktonic Euryarchaeota, and test if it is composed of different ecologically relevant units. We re‐analyzed Tara Oceans metagenomes from the photic layer and the deep ocean by annotating sequences against a custom MGII database and by mapping gene co‐occurrences. Our data provide a global view of the distribution of Euryarchaeota, and more...
Tipo: Text Palavras-chave: 16S rRNA; Euryarchaeota; Global ocean; Metagenomics; Poseidoniales; Proteorhodopsin.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00505/61676/65607.pdf
Imagem não selecionada

Imprime registro no formato completo
Holobionts and their hologenomes: Evolution with mixed modes of inheritance Genet. Mol. Biol.
Haag,Karen Luisa.
Abstract Symbioses are ubiquitous and have played an influential role in the evolution of life on Earth. Genomic studies are now revealing a huge diversity of associations among hosts and their microbiotas, allowing us to characterize their complex ecological and evolutionary dynamics. The different transmission modes and the asynchronous cell proliferation of the numerous symbionts associated with one host generate a genomic conflict ought to be solved. Two disputing views have been used to model and predict the outcome of such conflicts. The traditional view is based on community ecology, and considers that selection at the level of individuals is sufficient to explain longstanding associations among species. A new perspective considers that the host and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genomics; Metagenomics; Microbiota; Symbiosis; Holobiont.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200189
Imagem não selecionada

Imprime registro no formato completo
Metagenomic and metatranscriptomic analysis of the microbial community structure and metabolic potential of fermented soybean in Yunnan Province Ciênc. Tecnol. Aliment.
LIU,Xiao-Feng; LIU,Chen-Jian; ZENG,Xue-Qin; ZHANG,Hai-Yan; LUO,Yi-Yong; LI,Xiao-Ran.
Abstract Traditional fermented soybean contains a variety of microflora. To obtain a comprehensive and accurate understanding of the microbial community structure and metabolic potential of fermented soybean, comparative metagenomics and metatranscriptomics were performed on a sample of fermented soybean in Yunnan Province. Metagenomic DNA and metatranscriptomic RNA were sequenced using Illumina HiseqTM2500, which yielded a total of 92,192,276 reads, with an average read length of 150 bp and 38,798,262 paired-end sequences, with an average length of 151 bp. The results show that Providencia stuartii was the most abundant species and the genes of carbohydrates (2296, 13.30%), protein metabolism (1530, 8.86%), and amino acids and amino acid derivatives...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermented soybean; Metagenomics; Metatranscriptomics; Microbial community.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005000301
Imagem não selecionada

Imprime registro no formato completo
Metagenomic and metatranscriptomic analysis of the microbial community structure and metabolic potential of fermented soybean in Yunnan Province Ciênc. Tecnol. Aliment.
LIU,Xiao-Feng; LIU,Chen-Jian; ZENG,Xue-Qin; ZHANG,Hai-Yan; LUO,Yi-Yong; LI,Xiao-Ran.
Abstract Traditional fermented soybean contains a variety of microflora. To obtain a comprehensive and accurate understanding of the microbial community structure and metabolic potential of fermented soybean, comparative metagenomics and metatranscriptomics were performed on a sample of fermented soybean in Yunnan Province. Metagenomic DNA and metatranscriptomic RNA were sequenced using Illumina HiseqTM2500, which yielded a total of 92,192,276 reads, with an average read length of 150 bp and 38,798,262 paired-end sequences, with an average length of 151 bp. The results show that Providencia stuartii was the most abundant species and the genes of carbohydrates (2296, 13.30%), protein metabolism (1530, 8.86%), and amino acids and amino acid derivatives...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermented soybean; Metagenomics; Metatranscriptomics; Microbial community.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100018
Imagem não selecionada

Imprime registro no formato completo
Metagenomic sequencing of environmental DNA reveals marine faunal assemblages from the West Antarctic Peninsula ArchiMer
Cowart, Dominique; Murphy, Katherine R.; Cheng, C. -h. Christina.
The West Antarctic Peninsula (WAP) is the fastest warming region in Antarctica where climate impact on the cold-adapted marine ecosystem is already visible. To monitor faunal changes in remote vast bodies of Antarctic waters, efficient and informative tools are essential. High-throughput sequencing of environmental DNA (eDNA) has emerged as one such tool for monitoring biodiversity and ecosystems, as it increases detection sensitivity of taxa, and sampling is often simpler and less costly than traditional collection methods. We collected water samples from four WAP shallow (≤ 300 m) shelf regions, recovered the eDNA therein, and performed metagenomic shotgun sequencing and analyses to determine the effectiveness of this method to assess marine benthic...
Tipo: Text Palavras-chave: EDNA; Antarctica; Metagenomics; King crabs; Benthic invertebrates.
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00413/52490/53278.pdf
Imagem não selecionada

Imprime registro no formato completo
Molecular analysis of the bacterial diversity in a specialized consortium for diesel oil degradation Rev. Bras. Ciênc. Solo
Paixão,Douglas Antonio Alvaredo; Dimitrov,Mauricio Rocha; Pereira,Rodrigo Matheus; Accorsini,Fábio Raphael; Vidotti,Maria Benincasa; Lemos,Eliana Gertrudes de Macedo.
Diesel oil is a compound derived from petroleum, consisting primarily of hydrocarbons. Poor conditions in transportation and storage of this product can contribute significantly to accidental spills causing serious ecological problems in soil and water and affecting the diversity of the microbial environment. The cloning and sequencing of the 16S rRNA gene is one of the molecular techniques that allows estimation and comparison of the microbial diversity in different environmental samples. The aim of this work was to estimate the diversity of microorganisms from the Bacteria domain in a consortium specialized in diesel oil degradation through partial sequencing of the 16S rRNA gene. After the extraction of DNA metagenomics, the material was amplified by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR; Cloning; 16S rRNA; Metagenomics; Soil; Biosurfactants.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000300019
Imagem não selecionada

Imprime registro no formato completo
Selective elimination of chloroplastidial DNA for metagenomics of bacteria associated with the green alga Caulerpa Taxifolia (bryopsidophyceae) ArchiMer
Aires, Tania; Marba, Nuria; Serrao, Ester A.; Duarte, Carlos M.; Arnaud-haond, Sophie.
Molecular analyses of bacteria associated with photosynthetic organisms are often confounded by coamplification of the chloroplastidial 16S rDNA with the targeted bacterial 16S rDNA. This major problem has hampered progress in the characterization of bacterial communities associated to photosynthetic organisms and has limited the full realization of the potential offered by the last generation of metagenomics approaches. A simple and inexpensive method is presented, based on ethanol and bleach treatments prior to extraction, to efficiently discard a great part of chloroplastidial DNA without affecting the characterization of bacterial communities through pyrosequencing. Its effectiveness for the description of bacterial lineages associated to the green...
Tipo: Text Palavras-chave: 16S; Chloroplasts; Elimination; Green algae; Metagenomics.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00076/18739/16464.pdf
Imagem não selecionada

Imprime registro no formato completo
The insect reservoir of biodiversity for viruses and for antiviral mechanisms Anais da ABC (AABC)
OLMO,ROENICK P.; MARTINS,NELSON EDUARDO; AGUIAR,ERIC ROBERTO G.R.; MARQUES,JOÃO T.; IMLER,JEAN-LUC.
Abstract: Insects are the most diverse group of animals. They can be infected by an extraordinary diversity of viruses. Among them, arthropod-borne viruses (arboviruses) can be transmitted to humans. High-throughput sequencing of small RNAs from insects provides insight into their virome, which may help understand the dynamics of vector borne infectious diseases. Furthermore, investigating the mechanisms that restrict viral infections in insects points to genetic innovations that may inspire novel antiviral strategies.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antiviral immunity; Insects; Metagenomics; Restriction factors; RNA interference; Virome.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000600604
Registros recuperados: 10
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional