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| Registros recuperados: 23 | |
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| Cossa, Daniel; Garnier, Cedric; Buscail, Roselyne; Elbaz-poulichet, Francoise; Mikac, Nevenka; Patel-sorrentino, Nathalie; Tessier, Erwan; Rigaud, Sylvain; Lenoble, Veronique; Gobeil, Charles. |
| Methylation of mercury (Hg) is the crucial process that controls Hg biomagnification along the aquatic food chains. Aquatic sediments are of particular interest because they constitute an essential reservoir where inorganic divalent Hg (HgII) is methylated. Methylmercury (MeHg) concentrations in sediments mainly result from the balance between methylation and demethylation reactions, two opposite natural processes primarily mediated by aquatic microorganisms. Thus, Hg availability and the activity of methylating microbial communities control the MeHg abundance in sediments. Consistently, some studies have reported a significant positive correlation between MeHg and HgII or total Hg (HgT), taken as a proxy for HgII, in aquatic sediments using... |
| Tipo: Text |
Palavras-chave: Mercury; Methylmercury; Aquatic sediment; Methylation; Demethylation. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00171/28220/26520.pdf |
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| Almeida,Fabiana Greyce Oliveira; Aquino,Priscila Ferreira de; Souza,Afonso Duarte Leäo de; Souza,Antonia Queiroz Lima de; Vinhote,Sonia do Carmo; Mac-Cormick,Thais Messias; Silva,Marcelo Soares da Mota; Chalub,Sidney Raimundo Silva; Fischer,Juliana de Saldanha da Gama; Carvalho,Paulo Costa; Carvalho,Maria da Gloria da Costa. |
| BACKGROUND: DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world. RESULTS: Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2... |
| Tipo: Journal article |
Palavras-chave: Methylation; Colorectal cancer; Amazon; Epigenetic. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100050 |
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| Cardoso,Leila C.A.; Castaño,Jair A. Tenorio; Pereira,Hanna S.; Lima,Maria Angélica de F.D.; Santos,Anna Cláudia E. dos; Faria,Paulo S. de; Ferman,Sima; Seuánez,Héctor N.; Nevado,Julián B.; Almeida,José Carlos Cabral de; Lapunzina,Pablo; Vargas,Fernando R.. |
| The most frequent epigenetic alterations in Wilms tumor (WT) occur at WT2, assigned to 11p15. WT2 consists of two domains: telomeric domain 1 (DMRH19) that contains the IGF2 gene and an imprinted maternally expressed transcript (H19) and centromeric domain 2 (KvDMR) that contains the genes KCNQ1, KCNQ1OT1 and CDKN1C. In this work, we used pyrosequencing and MS-MLPA to compare the methylation patterns of DMRH19/KvDMR in blood and tumor samples from 40 WT patients. Normal constitutional KvDMR methylation indicated that most of the epigenetic alterations in WT occur at DMRH19. Constitutional DMRH19 hypermethylation (HM DMRH19) was observed in two patients with Beckwith-Wiedemann syndrome. Pyrosequencing and MS-MLPA showed HM DMRH19 in 28/34 tumor samples:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epigenetic; Histopathology; Methylation; MS-MLPA; Pyrosequencing. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500002 |
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| Rodrigues,Maria Gabriela Fontanetti; Soares,Murilo Racy; Gimenez,Daniele Fernanda Jovino; Fonseca,Larissa Fernanda Simielli; Torrieri,Erico; Ramos,Ester Silveira; Giuliatti,Silvana. |
| ABSTRACT Fig tree (Ficus carica L.) breeding programs using conventional methods, such as directed crosses, to obtain new cultivars, are unworkable in many countries, including Brazil. Consequently, genetic breeding through mutagenesis has emerged as an important line of research that can improve this crop, and be a significant source of information about this species and assist in the implementation of propagation projects and appropriate management. The aim of this study was to verify the existence of epigenetic variability attributable to DNA methylation in irradiated fig selections when compared both to each other and to the main commercial cultivar, “Roxo-de-Valinhos”, which had previously used methylation-sensitive amplified polymorphism (MSAP) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ficus carica; DMRs; Methylation; Plant breeding; Epigenetics. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000400285 |
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| Li,Zhongai; Li,Jie; Yanhua,Liu; Wang,Zicheng. |
| Background: Analytical techniques such as methylation-sensitive amplification polymorphism and high-performance liquid chromatography were used to detect variation in DNA methylation of mature Chrysanthemum leaves during the floral transition induced by short-day (SD) treatment. Results: For both early- and late-flowering cultivars, the time from the date of planting to the appearance of the capitulum bud and early blooming were significantly shorter than those of the control. The capitulum development of the early-flowering cultivar was significantly accelerated compared to the control, unlike the late-flowering cultivar. The DNA methylation percentage of leaves was significantly altered during flower development. For the early-flowering cultivar, DNA... |
| Tipo: Journal article |
Palavras-chave: Chrysanthemum; DNA; Methylation; Floral transition; Short day. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300009 |
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| SOUZA, M. M. de; NICIURA, S. C. M.; ROCHA, M. I. P.; PAN, Z.; ZHOU, H.; BRUSCADIN, J. J.; DINIZ, W. J. da S.; AFONSO, J.; OLIVEIRA, P. S. N. de; MOURÃO, G. B.; ZERLOTINI NETO, A.; COUTINHO, L. L.; KOLTES, J. E.; REGITANO, L. C. de A.. |
| Abstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested... |
| Tipo: Artigo de periódico |
Palavras-chave: Nelore; Epigenoma; Músculo de nelore; RRBS; GNAS; EBF3; Força de cisalhamento; Shear force; Epigenome; Muscle; Metilação; Bos Taurus; Bos Indicus; Músculo; Cattle; Methylation; DNA methylation. |
| Ano: 2022 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1144144 |
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| Fortes,F.P.; Kuasne,H.; Marchi,F.A.; Miranda,P.M.; Rogatto,S.R.; Achatz,M.I.. |
| Li-Fraumeni syndrome (LFS) is a rare, autosomal dominant, hereditary cancer predisposition disorder. In Brazil, the p.R337H TP53 founder mutation causes the variant form of LFS, Li-Fraumeni-like syndrome. The occurrence of cancer and age of disease onset are known to vary, even in patients carrying the same mutation, and several mechanisms such as genetic and epigenetic alterations may be involved in this variability. However, the extent of involvement of such events has not been clarified. It is well established that p53 regulates several pathways, including the thymine DNA glycosylase (TDG) pathway, which regulates the DNA methylation of several genes. This study aimed to identify the DNA methylation pattern of genes potentially related to the TDG... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Li-Fraumeni syndrome; TP53 gene; TDG; Methylation. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700610 |
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| Da,M.X.; Zhang,Y.B.; Yao,J.B.; Duan,Y.X.. |
| DNA hypomethylation may activate oncogene transcription, thus promoting carcinogenesis and tumor development. S-adenosylmethionine (SAM) is a methyl donor in numerous methylation reactions and acts as an inhibitor of intracellular demethylase activity, which results in hypermethylation of DNA. The main objectives of this study were to determine whether DNA hypomethylation correlated with vascular endothelial growth factor-C (VEGF-C) expression, and the effect of SAM on VEGF-C methylation and gastric cancer growth inhibition. VEGF-C expression was assayed by Western blotting and RT-qPCR in gastric cancer cells, and by immunohistochemistry in tumor xenografts. VEGF-C methylation was assayed by bisulfite DNA sequencing. The effect of SAM on cell apoptosis was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: VEGF-C; Methylation; S-adenosylmethionine; Gastric cancer. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201021 |
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| Zhou,X.Q.; Huang,S.Y.; Zhang,D.S.; Zhang,S.Z.; Li,W.G.; Chen,Z.W.; Wu,H.W.. |
| Reversion-inducing cysteine-rich protein with kazal motifs (RECK), a novel tumor suppressor gene that negatively regulates matrix metalloproteinases (MMPs), is expressed in various normal human tissues but downregulated in several types of human tumors. The molecular mechanism for this downregulation and its biological significance in salivary adenoid cystic carcinoma (SACC) are unclear. In the present study, we investigated the effects of a DNA methyltransferase (DNMT) inhibitor, 5-aza-2′deoxycytidine (5-aza-dC), on the methylation status of the RECK gene and tumor invasion in SACC cell lines. Methylation-specific PCR (MSP), Western blot analysis, and quantitative real-time PCR were used to investigate the methylation status of the RECK gene and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RECK; Salivary adenoid cystic carcinoma; 5-aza-2′deoxycytidine; Methylation; Transwell assay. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000300254 |
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| Anselmo,Nilson Praia; Bello,Maria Josefa; Gonzalez-Gomez,Pilar; Dias,Luis Antonio Araújo; Almeida,José Reinaldo Walter de; Santos,Marcelo José dos; Rey,Juan A.; Casartelli,Cacilda. |
| Aberrant methylation of CpG islands located in promoter regions represents one of the major mechanisms for silencing cancer-related genes in tumor cells. We determined the frequency of aberrant CpG island methylation for several tumor-associated genes: DAPK, MGMT, p14ARF, p16INK4a, TP73, RB1 and TIMP-3 in 55 brain tumors, consisting of 26 neuroepithelial tumors, 6 peripheral nerve tumors, 13 meningeal tumors and 10 metastatic brain tumors. Aberrant methylation of at least one of the seven genes studied was detected in 83.6% of the cases. The frequencies of aberrant methylation were: 40% for p14ARF, 38.2% for MGMT, 30.9% for, p16INK4a, 14.6% for TP73 and for TIMP-3, 12.7% for DAPK and 1.8% for RB1. These data suggest that the hypermethylation observed in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brain tumors; Epigenetics; Methylation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300001 |
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| PAIVA, J. T.; RESENDE, M. D. V. de; RESENDE, R. T.; OLIVEIRA, H. R.; SILVA, H. T.; CAETANO, G. C.; LOPES, P. S.; SILVA, F. F.. |
| A epigenética é definida como mudanças na expressão gênica que podem ser herdadas e que não alteram a sequência do DNA. Existem dois mecanismos epigenéticos principais, a metilação do DNA e a modificação de histonas. Também se destaca o imprinting genômico, o qual está relacionado com a regulação gênica quando apenas um alelo (herdado do pai ou da mãe) se expressa. A herança epigenética transgeracional consiste da herança de marcas epigenéticas através de células germinativas, que controla os padrões de expressão gênica e são passados de uma geração para a outra. Dentro do programa de melhoramento animal é importante avaliar a extensão com que a herança epigenética transgeracional também afeta a eficiência da seleção. Uma melhor compreensão dos mecanismos... |
| Tipo: Artigo de periódico |
Palavras-chave: Epigenoma; Expressão gênica; Histona; Transgeracional; Epigenome; Transgenerational; Metilação; Melhoramento Genético Animal; Gene expression; Methylation; Histones. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110162 |
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| PEREIRA, C. M.; CARVALHO, A. C. de; SILVA, F. R. da; MELENDEZ, M. E.; LESSA, R. C.; ANDRADE, V. C. C.; KOWALSKI, L. P.; VETTORE, A. L.; CARVALHO, A. L.. |
| Abstract. Background: Aberrant methylation is a frequent event in oral cancer. Methods: In order to better characterize these alterations, a search for genes downregulated by aberrant methylation in oral squamous cell carcinoma (OSCC) was conducted through the mining of ORESTES dataset. Findings were further validated in OSCC cell lines and patients? samples and confirmed using TCGA data. Differentially expressed genes were identified in ORESTES libraries and validated in vitro using RT-PCR in HNSCC cell-lines and OSCC tumor samples. Further confirmation of these results was performed using mRNA expression and methylation data from The Cancer Genome Atlas (TCGA) data. Results: From the set of genes selected for validation, CA3 and FHL1 were downregulated... |
| Tipo: Artigo de periódico |
Palavras-chave: Expressão gênica; ORESTES; Carcinogênese; Validação in vitro; Validação in silico; Open reading expressed sequence tags; Oral squamous cell carcinoma; Metilação; Gene expression; Methylation. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105692 |
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| Le Luyer, Jeremy; Laporte, Martin; Beacham, Terry D.; Kaukinen, Karia H.; Withler, Ruth E.; Leong, Jong S.; Rondeau, Eric B.; Koop, Ben F.; Bernatchez, Louis. |
| A puzzling question in conservation biology is how to maintain overall fitness of individuals bred in captive environment upon release into the wild, especially for rehabilitating declining or threatened species [1,2]. For salmonid species, a heritable change in fitness related traits and gene expression has been reported to occur in a single generation of captivity in hatchery environment [3–5]. Such rapid changes are congruent with models of inadvertent domestication selection which may lead to maladaptation in the natural environment [4]. Arguably, the underlying mechanism by which captivity may induce fitness difference between wild and captive congeners is still poorly understood. Short- term selection on complex phenotypic traits is expected to... |
| Tipo: Text |
Palavras-chave: Epigenetics; Methylation; Coho salmon; Hatchery; RAD sequencing. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00409/52050/52722.pdf |
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| Registros recuperados: 23 | |
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