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Analysis of microbial diversity in Shenqu with different fermentation times by PCR-DGGE BJM
Liu,Tengfei; Jia,Tianzhu; Chen,Jiangning; Liu,Xiaoyu; Zhao,Minjie; Liu,Pengpeng.
Abstract Shenqu is a fermented product that is widely used in traditional Chinese medicine (TCM) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. The aim of this study was to investigate microbial diversity in Shenqu using different fermentation time periods. DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles indicated that a strain of Pediococcus acidilactici (band 9) is the predominant bacteria during fermentation and that the predominant fungi were uncultured Rhizopus, Aspergillus oryzae, and Rhizopus oryzae. In addition, pathogenic bacteria, such as Enterobacter cloacae, Klebsiella oxytoca, Erwinia billingiae, and Pantoea vagan were detected in Shenqu. DGGE analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial diversity; PCR-DGGE; Shenqu; Molecular cloning.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200246
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Atividade enzimática e perfil da comunidade bacteriana em solo submetido à solarização e biofumigação. Repositório Alice
PASSOS, S.R.; REIS JUNIOR, F.B. dos; RUMJANEK, N.G.; MENDES, I. de C.; BAPTISTA, M.J.; XAVIER, G.R..
O objetivo deste trabalho foi avaliar o efeito da solarização e da biofumigação sobre a comunidade microbiana do solo, por meio da atividade da enzima beta-glicosidase e do perfil do 16S rDNA, determinado com PCR-DGGE. A solarização do solo, com cobertura de plástico, foi feita por períodos de dois, quatro e seis meses, e a biofumigação foi realizada pela incorporação de 2 e 5% (v/v) de cama-de-frango ao solo. Logo após a retirada da cobertura de plástico e aos 30 dias após a remoção, a atividade da beta-glicosidase foi menor em relação ao tratamento não solarizado. Aos 60 dias, não foram mais observadas diferenças entre os tratamentos. A adição de cama-de-frango a 5% estimulou a atividade da beta-glicosidase. O perfil da estrutura da comunidade bacteriana...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Beta-glicosidase; Cama-de-frango; Diversidade microbiana; Ecologia microbiana; Manejo do solo; 16S rDNA; Chicken litter; Microbial diversity; Microbial ecology; Soil management.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/122109
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Atividade enzimática e perfil da comunidade bacteriana em solos submetido à solarização e biofumigação. Repositório Alice
PASSOS, S. R.; REIS JÚNIOR, F. B. dos; RUMJANEK, N. G.; MENDES, I. de C.; BAPTISTA, M. J.; XAVIER, G. R..
O objetivo deste trabalho foi avaliar o efeito da solarização e da biofumigação sobre a comunidade microbiana do solo, por meio da atividade da enzima beta-glicosidase e do perfil do 16S rDNA, determinado com PCR-DGGE. A solarização do solo, com cobertura de plástico, foi feita por períodos de dois, quatro e seis meses, e a biofumigação foi realizada pela incorporação de 2 e 5% (v/v) de cama-de-frango ao solo. Logo após a retirada da cobertura de plástico e aos 30 dias após a remoção, a atividade da beta-glicosidase foi menor em relação ao tratamento não solarizado. Aos 60 dias, não foram mais observadas diferenças entre os tratamentos. A adição de cama-de-frango a 5% estimulou a atividade da beta-glicosidase. O perfil da estrutura da comunidade bacteriana...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Solarização; Beta-glicosidase; Cama-de-frango; Diversidade microbiana; Ecologia microbiana; Manejo do solo; Solarization; Beta-glucosidase; Chicken litter; Microbial diversity; Microbial ecology; Soil management.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/630059
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Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ) Anais da ABC (AABC)
COELHO-SOUZA,SERGIO A.; ARAÚJO,FÁBIO V.; CURY,JULIANO C.; JESUS,HUGO E.; PEREIRA,GILBERTO C.; GUIMARÃES,JEAN R.D.; PEIXOTO,RAQUEL S.; DÁVILA,ALBERTO M.R.; ROSADO,ALEXANDRE S..
ABSTRACTUpwelling systems contain a high diversity of pelagic microorganisms and their composition and activity are defined by factors like temperature and nutrient concentration. Denaturing gradient gel electrophoresis (DGGE) technique was used to verify the spatial and temporal genetic variability of Bacteria and Archaea in two stations of the Arraial do Cabo coastal region, one under upwelling pressure and another under anthropogenic pressure. In addition, biotic and abiotic variables were measured in surface and deep waters from three other stations between these stations. Six samplings were done during a year and adequately represented the degrees of upwelling and anthropogenic pressures to the system. Principal Component Analysis (PCA) showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial diversity; Leucine incorporation; Flow cytometry; Temperature; Nutrients availability; Brazilian Upwelling System.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401737
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Characterization of spoilage bacteria in pork sausage by PCR-DGGE analysis Ciênc. Tecnol. Aliment.
Dias,Francesca Silva; Ramos,Cíntia Lacerda; Schwan,Rosane Freitas.
To investigate microbial diversity and identify spoilage bacteria in fresh pork sausages during storage, twelve industrial pork sausages of different trademarks were stored at 4 ºC for 0, 14, 28 and 42 days, 80% relative humidity and packaged in sterile plastic bags. Microbiological analysis was performed. The pH and water activity (a w) were measured. The culture-independent method performed was the Polymerase Chain Reaction - Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The culture-dependent method showed that the populations of mesophilic bacteria and Lactic Acid Bacteria (LAB) increased linearly over storage time. At the end of the storage time, the average population of microorganisms was detected, in general, at the level of 5 log cfu g-1. A...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial diversity; Lactobacillus sakei; Brochothrix thermosphacta.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000300013
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Comparative Study of Guaymas Basin Microbiomes: Cold Seeps vs. Hydrothermal Vents Sediments ArchiMer
Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne.
In the Guaymas Basin, the presence at a few tens of kilometers of cold seeps and hydrothermal vents coupled with comparable sedimentary settings and depths offer a unique opportunity to assess and compare the microbial community composition of these deep-sea ecosystems. The microbial diversity in sediments from three cold seep and two hydrothermal vent assemblages were investigated using high-throughput 16S rRNA-sequencing. Numerous bacterial and archaeal lineages were detected in both cold seep and hydrothermal vent sediments. Various potential organic matter degraders (e.g., Chloroflexi, Atribacteria, MBG-D) and methane and sulfur cycling related microorganisms (e.g., ANME and methanogenic lineages, sulfate-reducing lineages) were detected in both...
Tipo: Text Palavras-chave: Microbial diversity; Hydrothermal vents; Cold seeps; Ribotypes; Methane; Extreme environments.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00415/52640/53496.pdf
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Comparison of different protocols for the extraction of microbial DNA from reef corals BJM
Santos,H.F.; Carmo,F.L.; Leite,D.C.A.; Jesus,H.E.; De Carvalho Maalouf,P.; Almeida,C.; Soriano,A.U.; Altomari,D.; Suhett,L.; Vólaro,V.; Valoni,E.; Francisco,M.; Vieira,J.; Rocha,R.; Sardinha,B.L.; Mendes,L.B.; João,R.R.; Lacava,B.; Jesus,R.F.; Sebastian,G.V.; Pessoa,A.; van Elsas,J.D.; Rezende,R.P.; Pires,D.O.; Duarte,G.; Castro,C.B.; Rosado,A.S.; Peixoto,R.S..
This study aimed to test different protocols for the extraction of microbial DNA from the coral Mussismilia harttii. Four different commercial kits were tested, three of them based on methods for DNA extraction from soil (FastDNA SPIN Kit for soil, MP Bio, PowerSoil DNA Isolation Kit, MoBio, and ZR Soil Microbe DNA Kit, Zymo Research) and one kit for DNA extraction from plants (UltraClean Plant DNA Isolation Kit, MoBio). Five polyps of the same colony of M. harttii were macerated and aliquots were submitted to DNA extraction by the different kits. After extraction, the DNA was quantified and PCR-DGGE was used to study the molecular fingerprint of Bacteria and Eukarya. Among the four kits tested, the ZR Soil Microbe DNA Kit was the most efficient with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coral; Microbial diversity; DNA extraction.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200012
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Continuous enrichment cultures: insights into prokaryotic diversity and metabolic interactions in deep-sea vent chimneys ArchiMer
Postec, Anne; Lesongeur, Francoise; Pignet, Patricia; Ollivier, B; Querellou, Joel; Godfroy, Anne.
The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga,...
Tipo: Text Palavras-chave: Metabolic interactions; 16S rRNA gene; Thermophiles; Bioreactor; Continuous enrichment cultures; Deep sea hydrothermal vent; Microbial diversity.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-3885.pdf
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Distribution of prokaryotic organisms in a tropical estuary influenced by sugar cane agriculture in northeast Brazil BJM
Wolf,Lars; Schwalger,Berit; Knoppers,Bastiaan A.; Silva,Luiz Antonio Ferreira da; Medeiros,Paulo Ricardo Petter; Pollehne,Falk.
In a joint Brazilian-German case study, distribution patterns of microorganisms were compared with environmental variables in the tropical coastal Manguaba lagoon in northeast Brazil, which is situated downstream of several sugar cane processing plants . 16S rDNA and 16S rRNA single strand conformation polymorphism (SSCP) gene fingerprinting were used to follow the composition and distribution of microorganisms throughout the salinity gradient of the lagoon. Potentially abundant microorganisms were identified by sequencing representative SSCP bands. It could be demonstrated that the distribution of microbes was in close relation to the physico-chemical environmental settings and followed a common scheme. In the in- and outlet areas of the lagoon rather...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tropical lagoon; Estuarine gradient; Microbial diversity; Sugar cane.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400007
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Effect of variation of environmental conditions on the microbial communities of deep-sea vent chimneys, cultured in a bioreactor ArchiMer
Byrne, Nathalie; Lesongeur, Francoise; Bienvenu, Nadege; Geslin, Claire; Alain, Karine; Prieur, Daniel; Godfroy, Anne.
Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N-2/H-2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85A degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60A degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences...
Tipo: Text Palavras-chave: 16S rRNA genes; Oligotrophic condition; Environmental condition; Bioreactor; Continuous enrichment culture; Microbial diversity; Deep sea hydrothermal vent.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6920.pdf
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Isolamento e caracterização morfofisiológica de fungos filamentosos de áreas mineradas. Repositório Alice
BATISTA, A. M.; MATTOS, B. B.; SANTOS, S. S.; OLIVEIRA, C. A.; OLIVEIRA, M. C. R.; TAKAHASHI, J. A.; SOUZA, F. A.; MARRIEL, I. E..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Área degradada; Morfofisiologia; Diversidade microbiana; Degraded areas; Morphophysiology; Microbial diversity.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1001577
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Lactose levels in diets for piglets weaned at 21 days of age R. Bras. Zootec.
Molino,Juliano Pelição; Donzele,Juarez Lopes; Oliveira,Rita Flávia Miranda de; Ferreira,Aloízio Soares; Moraes,Célia Alencar de; Haese,Douglas; Saraiva,Alysson; Oliveira,João Paulo de.
With the objective of evaluating levels of lactose in diets for piglets weaned at 21 days of age, it was carried out an experiment with 72 animals, with average weight of 6.12 kg, distributed in a completely randomized block design with four diets (0, 4, 8, and 12% lactose), six replicates, and 3 animals per experimental unit. Animals were fed the experimental diets from 21 to 35 days of age and they were fed a lactose-free basal diet from 36 to 49 days of age. The lactose levels did not affect daily weight gain, feed intake and feed conversion of piglets from 21 to 35 days of age. The use of lactose levels in the diets also did not affect performance of the piglets assessed from 21 to 49 days old. Diets also did not influence villous height, crypt depth...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Intestinal microbiota; Microbial diversity; Mucosal structure; Weaning.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000600011
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Land-use systems affect Archaeal community structure and functional diversity in western Amazon soils Rev. Bras. Ciênc. Solo
Navarrete,Acácio Aparecido; Taketani,Rodrigo Gouvêa; Mendes,Lucas William; Cannavan,Fabiana de Souza; Moreira,Fatima Maria de Souza; Tsai,Siu Mui.
The study of the ecology of soil microbial communities at relevant spatial scales is primordial in the wide Amazon region due to the current land use changes. In this study, the diversity of the Archaea domain (community structure) and ammonia-oxidizing Archaea (richness and community composition) were investigated using molecular biology-based techniques in different land-use systems in western Amazonia, Brazil. Soil samples were collected in two periods with high precipitation (March 2008 and January 2009) from Inceptisols under primary tropical rainforest, secondary forest (5-20 year old), agricultural systems of indigenous people and cattle pasture. Denaturing gradient gel electrophoresis of polymerase chain reaction-amplified DNA (PCR-DGGE) using the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soil microbial ecology; Soil microbiology; Microbial diversity; Land use changes; Tropical soils.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832011000500007
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Microbial communities and their biogeochemical role in the water column of the oxygen minimum zone in the eastern South Pacific Gayana
Ulloa,Osvaldo; Belmar,Lucy; Farías,Laura; Castro-González,Maribel; Galán,Alexander; Lavín,Paris; Molina,Verónica; Ramírez,Salvador; Santibáñez,Francisco; Stevens,Heike.
In recent years, our group has been studying the microbial communities of the water column associated with the oxygen minimum zone (OMZ) off northern Chile and Peru, trying to understand who the main players are in the OMZ biogeochemical cycles and which metabolic strategies they are using. For that, we are combining flow cytometry, molecular techniques and biogeochemical approaches. Most of our work, so far, has focused on microbes capable of doing oxygenic photosynthesis, such as the cyanobacteria, and those involved in the nitrogen cycle, such as the denitrifying, nitrifying and anammox bacteria. We have also started to look at the more general microbial abundance and diversity. In each case, we are finding that the OMZ holds distinct microbial...
Tipo: Journal article Palavras-chave: Oxygen minimum zone; Microbial diversity; Nitrogen cycle.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382006000300018
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Microbial communities in Cerrado soils under native vegetation subjected to prescribed fire and under pasture PAB
Viana,Laura Tillmann; Bustamante,Mercedes Maria da Cunha; Molina,Marirosa; Pinto,Alexandre de Siqueira; Kisselle,Keith; Zepp,Richard; Burke,Roger A.
The objective of this work was to evaluate the effects of fire regimes and vegetation cover on the structure and dynamics of soil microbial communities, through phospholipid fatty acid (PLFA) analysis. Comparisons were made between native areas with different woody covers ("cerrado stricto sensu" and "campo sujo"), under different fire regimes, and a 20-year-old active palisadegrass pasture in the Central Plateau of Brazil. Microbial biomass was higher in the native plots than in the pasture, and the highest monthly values were observed during the rainy season in the native plots. No significant differences were observed between fire regimes or between communities from the two native vegetation types. However, the principal component (PC) analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Land use; Microbial diversity; PLFA; Savannas; Seasonality.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011001200012
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Microbial diversity associated to the intestinal tract of soil invertebrates. Repositório Alice
CORREIA, D. da S.; PASSOS, S. R.; PROENÇA, D. N.; MORAIS, P. V.; XAVIER, G. R.; CORREIA, M. E. F..
Interactions between saprophagous invertebrates and microbes are essential for the maintenance and functioning of soil ecosystems, as they directly affect the degradation of organic matter and the nutrient cycle. The intestinal tract of invertebrates is inhabited by a diversity of microbes, and it is closely associated with the food ingested. The aim of this work was to evaluate the profile of prokaryotes associated with the intestinal tract of three invertebrate species. The species of invertebrates Trigoniulus corallinus was collected and incubated in the experiment, after 5 days of incubation we observed the uninduced colonization of two invertebrate species Cubaris murina and Pycnoscelus surinamensis.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Saprophagous; DGGE analysis; Microbial diversity; Invertebrates.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1098179
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Microbial diversity in a bagasse-based compost prepared for the production of Agaricus brasiliensis BJM
Silva,Cristina Ferreira; Azevedo,Raquel Santos; Braga,Claudia; Silva,Romildo da; Dias,Eustáquio Souza; Schwan,Rosane Freitas.
Edible mushrooms are renowned for their nutritional and medicinal properties and are thus of considerable commercial importance. Mushroom production depends on the chemical composition of the basic substrates and additional supplements employed in the compost as well as on the method of composting. In order to minimise the cost of mushroom production, considerable interest has been shown in the use of agro-industrial residues in the preparation of alternative compost mixtures. However, the interaction of the natural microbiota present in agricultural residues during the composting process greatly influences the subsequent colonisation by the mushroom. The aim of the present study was to isolate and identify the microbiota present in a sugar cane bagasse...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agaricus brasiliensis; Cane sugar bagasse; Microbial diversity.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300023
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Microbiological, technological and therapeutic properties of kefir: a natural probiotic beverage BJM
Leite,Analy Machado de Oliveira; Miguel,Marco Antonio Lemos; Peixoto,Raquel Silva; Rosado,Alexandre Soares; Silva,Joab Trajano; Paschoalin,Vania Margaret Flosi.
Kefir is a fermented milk beverage produced by the action of bacteria and yeasts that exist in symbiotic association in kefir grains. The artisanal production of the kefir is based on the tradition of the peoples of Caucasus, which has spread to other parts of the world, from the late 19th century, and nowadays integrates its nutritional and therapeutic indications to the everyday food choices of several populations. The large number of microorganisms present in kefir and their microbial interactions, the possible bioactive compounds resulting of microbial metabolism, and the benefits associated with the use this beverage confers kefir the status of a natural probiotic, designated as the 21th century yoghurt. Several studies have shown that kefir and its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Probiotic; Microbial diversity; Kefir grains; Fermented milk; Kefir.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200001
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Molecular characterization of bacterial populations of different soils BJM
Pereira,Rodrigo Matheus; Silveira,Érico Leandro da; Scaquitto,Denilson César; Pedrinho,Eliamar Aparecida Nascimbém; Val-Moraes,Silvana Pompéia; Wickert,Ester; Carareto-Alves,Lúcia Maria; Lemos,Eliana Gertrudes de Macedo.
Until recently, few studies were carried out in Brazil about diversity of bacterial soil communities. Aiming to characterize the bacterial population in the soil through 16S rRNA analysis, two types of soil have been analyzed: one of them characterized by intensive use where tomato, beans and corn were cultivated (CS); the other analyzed soil was under forest (FS), unchanged by man; both located in Guaíra, São Paulo State, Brazil. Using specific primers, 16S rRNA genes from metagenomic DNA in both soils were amplified by PCR, amplicons were cloned and 139 clones from two libraries were partially sequenced. The use of 16S rRNA analysis allowed identification of several bacterial populations in the soil belonging to the following phyla: Acidobacteria,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metagenome; Microbial diversity; 16S rRNA.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400007
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Origin of broiler carcass condemnations R. Bras. Zootec.
Muchon,José Luiz; Garcia,Rodrigo Garófallo; Gandra,Érika Rosendo de Sena; Assunção,Andrey Sávio de Almeida; Komiyama,Claudia Marie; Caldara,Fabiana Ribeiro; Nääs,Irenilza Alencar; Santos,Ricardo Antonio dos.
ABSTRACT The objective of the present research was to study the incidence of broiler carcass condemnations over eleven years and identify the productive phase that causes greater loss in slaughter process. The origin of these condemnations was determined before and after fasting. Evaluated broilers were reared in positive pressure warehouses and slaughtered between 28 and 34 days old with carcass weight ranging from 0.7 to 1.4 kg. Fasting occurred, on average, 9 h before the slaughter. Condemnation and slaughter data were collected from 2004 to 2014 in a slaughterhouse with slaughter capacity of 120,000 broilers/day. The causes of rearing condemnations were airsaccullitis, arthritis, abscess, ascites, cachexia, cellulitis, colibacillosis, dermatosis,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal production; Broilers; Microbial diversity; Slaughterhouse.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100523
Registros recuperados: 25
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