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| Registros recuperados: 30 | |
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| ROSSMANN, M.; PÉREZ-JARAMILLO, J. E.; CHIARAMONTE, J. B.; KAVAMURA, V. N.; DUMACK, K.; FIORE-DONNO, A. M.; MENDES, L. W.; BONKOWSKI, M.; MAUCHLINE, T.; RAAIJMAKERS, J. M.; MENDES, R.. |
| Domestication of plants may have impacted the interactions between plants and their rhizosphere microbiome. Considering that plants rely on the rhizosphere microbiome for functions related with plant growth, nutrition and health, the objective of this work was to analyse the rhizosphere microbiome of wild relatives and modern varieties of wheat and to investigate whether wild relatives recruit a higher proportion of beneficial microbes than modern cultivars. By 16S-rRNA gene amplicon, ITS and 18SrRNA gene amplicon, we assessed the bacterial, fungal and cercozoa rhizosphere community composition of five modern cultivars and three wheat ancestors cultivated in forest and agricultural soils. Results revealed a pronounced soil type and rhizosphere effect on... |
| Tipo: Separatas |
Palavras-chave: Wild wheat; Trigo; Rizosfera; Rhizosphere; Wheat; Microbiome. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103217 |
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| MENDES, L. W.; MENDES, R.; RAAIJMAKERS, J. M.; TSAI, S. M.. |
| Over the past century, plant breeding programs have substantially improved plant growth and health, but have not yet considered the potential effects on the plant microbiome. Here, we conducted a metatranscriptome analysis to determine if and how breeding for resistance of common bean against the root pathogen Fusarium oxysporum (Fox) affected gene expression in the rhizobacterial community. Our data revealed that rhizobacterial community assembly of Fox-resistant and susceptible bean cultivars follows niche-based mechanisms, presenting lower diversity and distinct community structure compared to the bulk soil. The microbiome of the Fox-resistant bean cultivar presented a significantly higher expression of bacterial genes associated with nutrient... |
| Tipo: Separatas |
Palavras-chave: Rizosfera; Feijão; Microbiome; Rhizosphere; Beans; Breeding. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103238 |
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| Hassan,Mariam; Essam,Tamer; Megahed,Salwa. |
| Abstract In this study, the development and assessment of a modified, efficient, and cost-efficient protocol for mDNA (metagenomic DNA) extraction from contaminated water samples was attempted. The efficiency of the developed protocol was investigated in comparison to a well-established commercial kit (Epicentre, Metagenomic DNA Isolation Kit for Water). The comparison was in terms of degree of shearing, yield, purity, duration, suitability for polymerase chain reaction and next-generation sequencing in addition to the quality of next-generation sequencing data. The DNA yield obtained from the developed protocol was 2.6 folds higher than that of the commercial kit. No significant difference in the alpha (Observed species, Chao1, Simpson and PD whole tree)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioinformatics; Method; Microbiome; Next-generation sequencing; QIIME. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500001 |
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| CARVALHO, J. C. de; COSTA, L. S. A. S.; CHIARAMONTE, J. B.; ROSSMANN, M.; MENDES, R.. |
| Resumo: O processo de domesticação do trigo, como outras espécies cultivadas, está relacionado à formação de civilizações sedentárias. A domesticação consiste na seleção de caracteres fenotípicos úteis para uso humano. A transição do ambiente natural para sistemas de produção modernos geram diversas mudanças no ambiente que podem acarretar em impactos na microbiota do solo cultivado. As plantas vivem em associação com microrganismos que auxiliam em funções como defesa, saúde e crescimento da planta, e esta associação deve-se a um longo processo de coevolução. Assumindo que existe a dependência dessa microbiota, acredita-se que cultivares modernos perderam algumas características para recrutar microrganismos específicos do hospedeiro durante o processo de... |
| Tipo: Separatas |
Palavras-chave: Domesticação de plantas; Microbioma da rizosfera; Interação planta-microbioma; Trigo; Rizosfera; Microbiome; Domestication; Wheat; Soil-plant interactions. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105014 |
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| KAVAMURA, V. N.; HAYAT, R.; CLARK, I. M.; ROSSMANN, M.; MENDES, R.; HIRSCH, P. R.; MAUCHLINE, T. H.. |
| The effects of fertilizer regime on bulk soil microbial communities have been well studied, but this is not the case for the rhizosphere microbiome. The aim of this work was to assess the impact of fertilization regime on wheat rhizosphere microbiome assembly and 16S rRNA gene-predicted functions with soil from the long term Broadbalk experiment at Rothamsted Research. Soil from four N fertilization regimes (organic N, zero N, medium inorganic N and high inorganic N) was sown with seeds of Triticum aestivum cv. Cadenza. 16S rRNA gene amplicon sequencing was performed with the Illumina platform on bulk soil and rhizosphere samples of 4-week-old and flowering plants (10 weeks). Phylogenetic and 16S rRNA gene-predicted functional analyses were performed.... |
| Tipo: Separatas |
Palavras-chave: Nitrogênio inorgânico; Trigo; Fertilizante nitrogenado; Impacto ambiental; Rizosfera; Wheat; Rhizosphere; Microbiome; Nitrogen fertilizers; Environmental impact; Bacterial communities; Functional diversity; Sustainable agricultural intensification. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1105404 |
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| KAVAMURA, V. N.; ROBINSON, R. J.; HAYAT, R.; CLARK, I. M.; HUGHES, D.; ROSSMANN, M.; HIRSCH, P. R.; MENDES, R.; MAUCHLINE, T. H.. |
| Abstract: Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also a discriminatory factor for the culture-independent-based method. Although culture-independent methods provide a higher resolution of analysis, it was found that in the... |
| Tipo: Artigo de periódico |
Palavras-chave: Endosphere; Rizosfera; População Microbiana; Manejo do Solo; Trigo; Semente; Rhizosphere; Microbiome; Wheat; Seeds. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1115412 |
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| Dubé, Caroline; Ky, Chin-long; Planes, Serge. |
| Elucidating the role of prokaryotic symbionts in mediating host physiology has emerged as an important area of research. Since oysters are the world’s most heavily cultivated bivalve molluscs, numerous studies have applied molecular techniques to understand the taxonomic and functional diversity of their associated bacteria. Here, we expand on this research by assessing the composition and putative functional profiles of prokaryotic communities from different organs/compartments of the black-lipped pearl oyster Pinctada margaritifera, a commercially important shellfish valued for cultured pearl production in the Pacific region. Seven tissues, in addition to mucous secretions, were targeted from P. margaritifera individuals: the gill, gonad, byssus gland,... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Microbiome; Tissue-specific bacterial communities; 16S rRNA gene sequencing; Functional profiling prediction. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00505/61670/65589.pdf |
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| CAMARGO, A. P.; SOUZA, R. S. C. de; COSTA, P. de B.; GERHARDT, I. R.; DANTE, R. A.; TEODORO, G. S.; ABRAHÃO, A.; LAMBERS, H.; CARAZZOLLE, M. F.; HUNTEMANN, M.; CLUM, A.; FOSTER, B.; FOSTER, B.; ROUX, S.; PALANIAPPAN, K.; VARGHESE, N.; MUKHERJEE, S.; REDDY, T. B. K.; DAUM, C.; COPELAND, A.; CHENM U, M. A.; IVANOVA, N. N.; KYRPIDES, N. C.; PENNACCHIO, C.; ELOE-FADROSH, E. A.; ARRUDA, P.; OLIVEIRA, R. S.. |
| The rocky, seasonally-dry and nutrient-impoverished soils of the Brazilian campos rupestres impose severe growth-limiting conditions on plants. Species of a dominant plant family, Velloziaceae, are highly specialized to low-nutrient conditions and seasonal water availability of this environment, where phosphorus (P) is the key limiting nutrient. Despite plant-microbe associations playing critical roles in stressful ecosystems, the contribution of these interactions in the campos rupestres remains poorly studied. Here we present the first microbiome data of Velloziaceae spp. thriving in contrasting substrates of campos rupestres. We assessed the microbiomes of Vellozia epidendroides, which occupies shallow patches of soil, and Barbacenia macrantha, growing... |
| Tipo: Artigo de periódico |
Palavras-chave: Microbioma de plantas; Campos rupestres brasileiros; Sequenciamento genético; Condições extremas; Solos; Stressful environments; DNA sequencing; Soils; Microrganismo; Velloziaceae; Microorganisms; Microbiome. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1111238 |
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| Clerissi, Camille; De Lorgeril, Julien; Petton, Bruno; Lucasson, Aude; Escoubas, Jean Michel; Gueguen, Yannick; Dégremont, Lionel; Mitta, Guillaume; Toulza, Eve. |
| Pacific Oyster Mortality Syndrome (POMS) affects Crassostrea gigas oysters worldwide and causes important economic losses. Disease dynamic was recently deciphered and revealed a multiple and progressive infection caused by the Ostreid herpesvirus OsHV-1 μVar, triggering an immunosuppression followed by microbiota destabilization and bacteraemia by opportunistic bacterial pathogens. However, it remains unknown if microbiota might participate to protect oysters against POMS, and if microbiota characteristics might be predictive of oyster mortalities. To tackle this issue, we transferred full-sib progenies of resistant and susceptible oyster families from hatchery to the field during a period in favor of POMS. After 5 days of transplantation, oysters from... |
| Tipo: Text |
Palavras-chave: Holobiont; Microbiome; Metabarcoding; Fitness; Bacteria. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00621/73332/72500.pdf |
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| CARRION, V. J.; PÉREZ-JARAMILLO, J.; CORDOVEZ, V.; HOLLANDER, M. de; TRACANNA, V.; MENDES, L. W.; MENDES, R.; MEDEMA, M. H.; RAAIJMAKERS, J. M.. |
| Endophytic microbes have been proposed to contribute to a range of plant support functions, including nutrient acquisition and disease protection. To date, the molecular mechanisms underlying disease protection in the endosphere have only been studied for individual culturable organisms. Here, we conducted a systematic microbiome-wide perspective on the taxonomic diversity and functional potential of the endophytic microbiome of plants grown in an agricultural soil that is naturally suppressive to damping-off disease caused by the fungal root pathogen Rhizoctonia solani. Metagenomic analyses showed an enrichment of Burkholderiaceae, Chitinophagaceae and Xanthomonadaceae in the endosphere of plants grown in disease-suppressive soil challenged with the... |
| Tipo: Separatas |
Palavras-chave: Microbiome; Antifungal agents; Metagenomics. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1103500 |
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| PAZ VENTERO, M.; CUADRAT, R. R. C.; VIDAL, I.; ANDRADE, B. G. N.; MOLINA-PARDINES, C.; HARI-MORENO, J. M.; COUTINHO, F. H.; MERINO, E.; REGITANO, L. C. de A.; SILVEIRA, C. B.; AFLI, H.; LÓPEZ-PÉREZ, M.; RODRÍGUEZ, J. C.. |
| Background: SARS-CoV-2 is an RNA virus causing COVID-19. The clinical characteristics and epidemiology of COVID-19 have been extensively investigated, however, only one study so far focused on the patient?s nasopharynx microbiota. In this study we investigated the nasopharynx microbial community of patients that developed different severity levels of COVID-19. We performed 16S ribosomal DNA sequencing from nasopharyngeal swab samples obtained from SARS-CoV-2 positive (56) and negative (18) patients in the province of Alicante (Spain) in their first visit to the hospital. Positive SARS-CoV-2 patients were observed and later categorized in mild (symptomatic without hospitalization), moderate (hospitalization), and severe (admission to ICU). We compared the... |
| Tipo: Artigo de periódico |
Palavras-chave: COVID 19; SARS CoV 2; NGS next generation sequencing; Coronavirus; Microbiome; Prevotella. |
| Ano: 2021 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1132766 |
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| SOUZA, R. C.; CANTAO, M. E.; NOGUEIRA, M. A.; VASCONCELOS, A. T. R.; HUNGRIA, M.. |
| Abstract: The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the... |
| Tipo: Artigo de periódico |
Palavras-chave: Metagenome; Manejo do Solo; Plantio Direto; Microbiologia do Solo; Soil management; Soil enzymes; No-tillage; Metagenomics; Microbiome. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100838 |
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| Souza,Renata Carolini; Cantão,Maurício Egídio; Nogueira,Marco Antonio; Vasconcelos,Ana Tereza Ribeiro; Hungria,Mariangela. |
| ABSTRACT The soil represents the main source of novel biocatalysts and biomolecules of industrial relevance. We searched for hydrolases in silico in four shotgun metagenomes (4,079,223 sequences) obtained in a 13-year field trial carried out in southern Brazil, under the no-tillage (NT), or conventional tillage (CT) managements, with crop succession (CS, soybean/wheat), or crop rotation (CR, soybean/maize/wheat/lupine/oat). We identified 42,631 hydrolases belonging to five classes by comparing with the KEGG database, and 44,928 sequences by comparing with the NCBI-NR database. The abundance followed the order: lipases > laccases > cellulases > proteases > amylases > pectinases. Statistically significant differences were attributed to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metagenome; Microbiome; Soil enzymes; Soil management; No-tillage. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400723 |
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| Clauss,Marcus; Hummel,Jürgen. |
| ABSTRACT Herbivores face the dilemma that the level of feed intake is negatively related to factors that determine digestive efficiency, such as thoroughness of ingesta comminution by chewing, and retention of digesta in the digestive tract. Ruminants have evolved particular adaptations to solve this dilemma. Most ruminants share the characteristic of “digesta washing”: fluid moves through their digestive tract faster than particles, thus effectively washing very fine particles, such as bacteria, out of the digesta plug. As the forestomach is followed by auto-enzymatic digestion, this allows a continuous, increased harvest of microbes from the forestomach. True rumination only evolved twice, in the camelids and the true ruminants. These both evolved a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Digesta washing; Foregut fermenter; Microbial harvest; Microbiome. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000700606 |
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| Registros recuperados: 30 | |
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