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A universal microscope manipulator Rev. Bras. entomol.
Boyadzhiev,Peter S.; Gechev,Tsanko S.; Donev,Atanas D..
A modified and improved model of a mechanical manipulator for observation of pinned and mounted insects is described. This device allows movement of the observed object around three perpendicular axes in the field of vision at all magnifications of stereomicroscopes. The main improvement of this new model is positioning of the guiding knobs for rotating around two of the axes next to each other, allowing faster and easier manipulation of the studied object. Thus, one of the main advantages of this device is the possibility to rotate the specimen without the need to refocus. The device enables easily reaching a precession deviation in the intersection point of axes up to 0.5 mm in the process of assembling.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Improved equipment; Microscopy; Mounted insects; Specimen manipulation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262012000100022
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Academic Achievement and Perception of Two Teaching Methods in Histology: Light Microscopy and Digital Systems International Journal of Morphology
Becerra G,Daniela; Grob L. P,Melisa; Rodríguez R,Ángel; Barker M,María José; Consiglieri L,Lucas; Ferri G,Giorgio; Sabag S,Natividad.
With the advent of digital systems, the role of the microscope as an irreplaceable instrument in the practical teaching of histology has been called into question. In this study academic performance and student perception for three learning methods was compared: digital systems, microscopy, and microscopy plus digital systems, in the muscle tissue unit of the morphology course for first-year dentistry at the Universidad de los Andes, Santiago, Chile. Ninety-five students were divided into 3 groups: Group 1: individual optical microscopy, Group 2: digital systems (one projector per room), and Group 3: microscopy plus digital systems. All participants observed the same striate muscle, cardiac striated muscle, and smooth muscle mounts. Their diagnostic...
Tipo: Journal article Palavras-chave: Medical education; Histology; Visual learning; Virtual slides; Microscopy.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300001
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Anti-erythrocyte antibodies may contribute to anaemia in Plasmodium vivax malaria by decreasing red blood cell deformability and increasing erythrophagocytosis. Repositório Alice
MOURÃO, L. C.; ROMA, P. M. da S.; ABOOBACAR, J. da S. S.; MEDEIROS, C. M. P.; ALMEIDA, Z. B. de; FONTES, C. J. F.; AGERO, U.; MESQUITA, O. N. de; BEMQUERER, M. P.; BRAGA, E. M..
bitstream/item/180098/1/s12936-016-1449-5.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Non-infected RBC; Auto-antibodies; Erythrophagocytosis; Defocusing; Anaemia; Microscopy; Plasmodium vivax.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1055374
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Appearance and Localization of Plasmodium yoelii Circumsporozoite Protein during Sporogony in Anopheles stephensi OAK
Torii, Motomi; Yanagi, Tetsuo; Tsuboi, Takafumi; Kanbara, Hiroji.
Palavras-chave: Circumsporozoite protein; Immunoelectron; Microscopy; Plasmodium yoelii; Sporogony.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/174
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Cellular Changes in the Placenta in Pregnancies Complicated with Diabetes International Journal of Morphology
Verma,Ranjana; Mishra,Sabita; Kaul,Jagat Mohini.
Placenta is the most accurate record of the infant prenatal experience. After delivery if the placenta is examined minutely, it provides much insight into the prenatal health of the baby and the mother. In diabetic pregnancy, placental weight is higher in comparison to normal pregnancy. To study the cellular differences that might contribute to larger size of placenta, light microscopic analysis was performed on 25 full term placentas, out of which 20 were of Gestational diabetes mellitus (12 controlled on diet, 8 controlled on insulin) and 5 control group. Tissue sections were processed and analyzed. Birth weight of neonate, placental weight, vascular pattern of chorionic blood vessels and site of attachment of umbilical cord were recorded. In the...
Tipo: Journal article Palavras-chave: Placenta; Gross; Gestational diabetes; Microscopy.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100038
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Chemical, structural and proteomic profile of buffalo milk powder produced in mini spray dryer. Repositório Alice
BORGES, M. V.; ALVES, M. F.; CHAVES, M. A.; EGITO, A. S. do; GROSS, E.; FERRÃO, S. P. B..
Abstract: The objective of this work was to obtain buffalo milk powder through the process of drying in mini spray dryer, and evaluate its physical, chemical, structural and proteomic features during storage. The buffalo milk was subjected to drying by atomization, air temperature of 140°C inlet. The samples were evaluated at times 0, 30, 60, 90, 120 and 150 days of storage. The moisture content, fat, protein, ash, density, acidity, solubility index, burning particles were analysed, along with structural analysis by Scanning Electron Microscopy (SEM) and proteomic study (SDS-PAGE). The production of milk powder from the set standards produced a buffalo milk powder with satisfactory results of physical and chemical compositions. In the SEM analysis was...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Leite de búfala; Búfalo; Tecnologia de alimento; Microscopia; Eletroforese; Composição química; Atomização; Armazenamento; Buffalo milk; Microscopy; Electrophoresis; Food composition; Chemical composition; Spray drying; Storage; Food technology.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1069628
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Closely-related Xanthomonas citri subsp. citri isolates trigger distinct histological and transcriptional responses in Citrus limon Scientia Agricola
Orce,Ingrid Georgina; Debes,Mario; Sendín,Lorena; Luque,Ana; Arias,Marta; Vojnov,Adrián; Marano,María; Castagnaro,Atilio; Filippone,María Paula.
ABSTRACT Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), has an important economic impact on the citrus industry. Extensive information is available about the disease but, nevertheless, the study of plant-pathogen interactions could provide new information in the understanding of citrus canker disease. A new isolate has been identified, Xcc AT, which has a high genetic similarity (> 90 %) to the virulent Xcc T strain based on genetic clustering analyses of the rep-PCR fingerprinting patterns, but it does not produce cankerous lesions in Citrus limon. In this study, we compared C. limon responses to Xcc AT and to the virulent Xcc T strain at both histological and transcriptional levels. Histologically, leaves inoculated with Xcc AT...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CDNA-AFLP; Gene expression; Canker; Plant defense; Microscopy.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000600552
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Comparative anatomy and histochemistry of the leaf blade of two species of Artocarpus Anais da ABC (AABC)
SÁ,RAFAELA D.; CADENA,MARÍLIA B.; PADILHA,RAFAEL J.R.; ALVES,LUIZ CARLOS; RANDAU,KARINA P..
Abstract Abstract: In Brazil, there are two species of Artocarpus that were introduced: Artocarpus altilis (Parkinson) Fosberg, known as fruta-pão, and Artocarpus heterophyllus Lam., known as jaca. Both are used as food and medicine. The objective of this work was to conduct a comparative anatomical and histochemical study between A. altilis and A. heterophyllus. Techniques of optical, polarized and scanning electron coupled to energy dispersive spectroscopy. The anatomical characterization showed the characters of general occurrence in the family Moraceae and of those that allow the differentiation of A. altilis and A. heterophyllus. The histochemistry revealed the sites of synthesis and/or storage of the metabolites. The chemical microanalyses brought...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artocarpus altilis; Artocarpus heterophyllus; Crystals; Microscopy; Moraceae.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100851
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Correlation between rheological properties and capillary fixation for modelling creams BJPS
Demoliner,Lara Brogni; Agnol,Lucas Dall; Zanotti,Suzana Bittencourt; Dias,Fernanda Trindade Gonzalez; Angeli,Valeria Weiss; Bianchi,Otavio.
The Brazilian male beauty market occupies the second place in world consumption of cosmetics. Among the numerous products consumed by such audience is the capillary fixation mask, which is mainly composed by fixatives. These additives act on stabilizing the cosmetic emulsion, protecting the hair against moisture and also increasing the intensity of hair fixation. In this work, three formulations for modelling creams were prepared by different concentrations of the fixatives StylezeTM W20 and PVP K90 and their properties characterized by physicochemical, rheological and sensory analysis. The capillary masks produced were stable oil-in-water (O/W) emulsions with uniform droplets of 2.05-2.82 μm sizes and pseudoplastic thixotropic behavior (0.19 < n <...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rheology; Microscopy; N-vinyl-2-pyrrolidone; Polyquaternium-55; Modelling cream/physicochemical properties; Cosmetics.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000300621
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Effect of an Enzymatic Blend and Yeast on the Performance, Carcass Yield and Histomorphometry of the Small Intestine in Broilers from 21 to 42 Days of Age Rev. Bras. Ciênc. Avic.
Sousa,RF; Dourado,LRB; Lopes,JB; Fernandes,ML; Kato,RK; Nascimento,DCN; Sakomura,NK; Lima,SBP; Ferreira,GJBC.
ABSTRACT The present study was conducted to evaluate the performance, carcass yield, and histomorphometry of the small intestine of broilers fed yeast sugarcane supplemented with enzymatic blend from 22 to 42 days of age. Seven hundred broiler chicks were distributed in a completely randomized design in a factorial design (2x3+1), two levels of the enzyme blend (0 and 200 g/ton), three protein levels (0%, 6% and 12%) and a control diet. The level of 6% yeast showed higher feed intake and weight gain after 33 days. From 22 to 42 days there was no significant effect (p<0.05) to the use of yeast in animal performance, carcass yield and cuts. At 42 days an interaction between the factors for width and crypt depth in the duodenum and jejunum, respectively,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cane sugar; Microscopy; Metabolism; Saccharomyces cerevisiae; Xylanase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200300
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Effect of Phytoaccumulation of Arsenic and Chromium on Structural and Ultrastructural Changes of Brake Fern (Pteris vittata) Braz. J. Plant Physiol.
Sridhar,Balaji B. Maruthi; Han,Fengxiang X.; Diehl,Susan V.; Monts,David L.; Su,Yi.
Structural and ultrastructural changes caused by bioaccumulation of As and Cr in brake fern (Pteris vittata), a known arsenic hyperaccumulator, were investigated. Potted plants of brake fern were exposed to metal treatments of As and Cr for three weeks. Leaf, stem and root samples were collected periodically and fixed for LM (Light Microscopy), SEM (Scanning Electron Microscopy) and TEM (Transmission Electron Microscopy) to evaluate anatomical changes. The fresh weights, dry weights, RWC (Relative Water Content) and plant heights were obtained before the brake fern plants were harvested for metal accumulation analysis. The As accumulated mainly in the shoots while Cr accumulated mainly in the roots of the metal-treated plants. Significant changes in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Arsenic; Chromium; Hyperaccumulator; Microscopy; Phytoremediation; Pteris vittata; Translocation; Ultrastructure.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000400006
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Effects of salinity on the physiology of the red macroalga, Acanthophora spicifera (Rhodophyta, Ceramiales) Acta Botanica
Pereira,Débora Tomazi; Simioni,Carmen; Filipin,Elisa Poltronieri; Bouvie,Fernanda; Ramlov,Fernanda; Maraschin,Marcelo; Bouzon,Zenilda Laurita; Schmidt,Éder Carlos.
ABSTRACT Salinity is an important abiotic factor since it is responsible for the local and/or regional distribution of algae. In coastal regions, salinity changes with prevailing winds, precipitation and tide, and particularly in extreme intertidal conditions. Acanthophora spicifera is a red seaweed that occurs in the supratidal region in which changes in abiotic conditions occur frequently. This study evaluated the effects of salinity on the metabolism and morphology of A. spicifera. Algae were acclimatized under culture conditions with sterilized seawater for seven days. Experiments used different salinities (15 to 50 psu) for seven days, followed by metabolic analyses. This study demonstrates that extreme salinities affect physiological parameters of A....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acanthophora spicifera; Microscopy; Morphology; Physiology; Salinity.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000400555
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Effects of Zn and Cd accumulation on structural and physiological characteristics of barley plants Braz. J. Plant Physiol.
Sridhar,Balaji B. Maruthi; Han,Fengxiang X.; Diehl,Susan V.; Monts,David L.; Su,Yi.
The objectives of this study were to identify the structural changes caused by Zn and Cd accumulation in shoots and roots of barley (Hordeum vulgare) plants; and to correlate metal accumulation with anatomical, physiological and morphological changes. Potted plants were exposed to metal treatments of Zn and Cd for 19 and 16 d respectively. Leaves, stems and roots were harvested to identify structural changes and analyze metal accumulation. Barley effectively accumulated Zn (up to 11283 mg kg-1) and Cd (up to 584 mg kg-1) in the shoots. Microscopic structural changes, such as a decrease in intercellular spaces, breakdown of vascular bundles, and shrinkage of palisade and epidermal cells, occurred in leaves, stems and roots of plants treated with high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Cadmium; Hordeum vulgare; Microscopy; Phytoremediation; Zinc.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000100002
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Electron Microscopic Evaluation of the Secretory Mechanisms of Renin from Juxtaglomerular Cells International Journal of Morphology
Aktas,Ranan Gulhan; Karabay,Gulten; Taskinalp,Oguz; Kutlu,A. Kemal.
Although the structure and the functions of juxtaglomerular cells (JG) have been well defined, there is still a controversy about the secretory mechanisms of renin from these cells. It has been assumed that exocytosis is the main secretory mechanism in these cells in many studies, while others suggest that secretion occurs in a quite different way in these cells. There are several studies suggesting that diacrine secretion, which is very difficult to visualize, might be the other mechanism for secretion of renin. This study is an attempt to find the answers of these questions by identifying the fine structural features of the secretory granules in juxtaglomerular cells. Cyclosporin A (CyA) has been used in the current experimental study since it has...
Tipo: Journal article Palavras-chave: Juxtaglomerular cells; Secretory granules; Microscopy.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300011
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Estrogen Receptors in Immunohistochemically Stained Slides: Comparing Subjective and Computerized Evaluation Methods International Journal of Morphology
Millas,Ieda; Barros,Mirna Duarte; Curcio,Daniella Franco; Buck,Hudson Sousa; Liquidato,Bianca Maria.
Immunohistochemistry is a specific and sensitive staining method for detection of several proteins. One important function of this method is to help us on the diagnosis of the presence of specific receptors as the estrogen receptors. The aim of this study was to compare two different methods to evaluate immunohistochemical staining intensity to detect the presence of ER in the nasal mucosa tissue: one using a digital system and the other through conventional direct microscopy observation. Sixty two stained samples were observed and analyzed under optic microscopy by three specialized professionals, who have graded intensity from: absent, mild, moderate and intense staining. Afterwards, an objective measurement was obtained by a relative optical density...
Tipo: Journal article Palavras-chave: Immunohistochemistry; Estrogen receptors; Methods; Microscopy.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400007
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Evaluation of the different irrigation regimens with sodium hypochlorite and EDTA in removing the smear layer during root canal preparation. Repositório Alice
SILVEIRA, L. F. M.; SILVEIRA, C. F.; MARTOSA, J.; CASTRO, L. A. S. de.
2013
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Dentine; Root canal preparation; Microscopy; Smear layer.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/980894
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Fruit salad as a new vehicle for probiotic bacteria Ciênc. Tecnol. Aliment.
MARTINS,Eliane Maurício Furtado; RAMOS,Afonso Mota; MARTINS,Maurilio Lopes; LEITE JÚNIOR,Bruno Ricardo de Castro.
Abstract This work aimed to study the use of fruit salads as carriers for Lactobacillus rhamnosus HN001. We evaluated the viability of this probiotic in fruit salads and the phsyico-chemical, microbiological and sensory properties of this food. Scanning electron microscopy (SEM) was used to verify microorganism adhesion on the fruit tissues. The viability of L. rhamnosus in fruit salads was 8.49 log CFU.g-1 after 120 hours. SEM images showed that fruit tissue provided protection for probiotic. Adhesion sites were observed in higher quantity in banana, apple and guava. The addition of L. rhamnosus did not alter texture of fruits (p > 0.05). Fruit salads containing probiotic had different values of pH and acidity compared to the control (p < 0.05)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acceptability; Fruit mix; Microscopy; Probiotic carrier.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000300540
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Germination effects on diversity of seed-associated bacteria. Repositório Alice
FERREIRA, A.; GONZÁLES, E. R.; AZEVEDO, J. L. de; ARAÚJO, W. L. de.
2011
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Plant-microbe interaction; Microscopy; DGGE; Germination.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/906989
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Granules morphology and rheological behavior of green banana (Musa cavendishii) and corn (Zea mays) starch gels Ciência e Agrotecnologia
Izidoro,Dayane Rosalyn; Demczuk Junior,Bogdan; Haminiuk,Charles Windson Isidoro; Sierakowski,Maria Rita; Freitas,Renato João Sossela de; Scheer,Agnes de Paula.
In this work, it was used starch obtained from green banana (Musa cavendishii) and commercial corn (Zea mays) starch in order to compare the granule morphology and the rheological behavior of these gel-starches. Images of starch granules morphology were obtained from scanning electron microscope (SEM). The banana starch granules presented an oval and ellipsoidal shape with irregular diameters. Neverthless, the granules of corn starch showed a poliedric shape, with different sizes. The rheological behavior of gel starch solutions showed a non-newtonian character with a pseudoplastic behavior. Herschel-Bulkley model gave a good description on the rheological behavior of the gel starch. Banana gel-starch solutions showed higher values of shear stress and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Starch; Green banana; Corn; Microscopy; Rheology.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542007000500026
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Hepato-pulmonary amebiasis: a case report BJID
Shenoy,Vishnu Prasad; Vishwanath,Shashidhar; Indira,Bairy; Rodrigues,G.
Infections with Entamoeba histolytica are seen worldwide and are more prevalent in the tropics. About 90% of infections are asymptomatic, and the remaining 10% produce a spectrum of clinical syndromes, ranging from dysentery to abscesses of the liver or other organs. Extra-intestinal infection by E. histolytica most often involves liver. Pleuro-pulmonary involvement, seen as the second most common extra-intestinal pattern of infection, is frequently associated with amebic liver abscess. Pulmonary amebiasis occurs in about 2-3% of patients with invasive amebiasis. We report herein the case of a 45-year-old male presenting with hepato-pulmonary amebiasis. The diagnosis was established from direct examination of sputum, in which trophozoites of E. histolytica...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Entamoeba histolytica; Liver abscess; Microscopy; Pulmonary involvement.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000400010
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