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Acute toxicity of Jatropha Curcas oil on plant cell cycle. Repositório Alice
ANDRADE-VIEIRA, L. F.; BOTELHO, C. M.; LAVIOLA, B. G.; FERREIRA, A.; PRAÇA-FONTES, M. M..
Tipo: Separatas Palavras-chave: Aneugenic effects; Mitotic index; Phorbol esters; Lactuca Sativa; Root growth.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114521
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Assessment of the antiproliferative and antigenotoxic activity and phytochemical screening of aqueous extracts of Sambucus australis Cham. & Schltdl. (ADOXACEAE) Anais da ABC (AABC)
TEDESCO,MARÍLIA; KUHN,ANDRIELLE W.; FRESCURA,VIVIANE DAL-SOUTO; BOLIGON,ALINE A.; ATHAYDE,MARGARETH L.; TEDESCO,SOLANGE B.; SILVA,ANTONIO C.F. DA.
ABSTRACT The purpose of this study was to evaluate the antiproliferative and antigenotoxic activity of Sambucus australis Cham. & Schltdl. aqueous extracts on the cell cycle of Allium cepa L. as well as determine the phenolic compounds in such extracts. S. australis inflorescences and leaves of two accessions were used for aqueous extract preparation at concentrations: 0.003 g/ml and 0.012 g/ml. A. cepa bulbs were rooted in distilled water and, subsequently, placed in treatments for 24 hours. Rootlets were collected and fixed in modified Carnoy’s solution for 24 hours and kept. The squash technique was performed for slide preparation. Root tips were smashed and stained with 2% acetic orcein, and a total of 4000 cells per treatment were analyzed. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plants; Mitotic index; Allium cepa; Sabugueiro; High-performance liquid chromatography.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502141
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Cytogenotoxicity of rice crop water after application of the tricyclazole fungicide Anais da ABC (AABC)
WANDSCHEER,ALANA C.D.; MARCHESAN,ENIO; TEDESCO,SOLANGE B.; FRESCURA,VIVIANE DAL-SOUTO; SOARES,CAMILLE F.; LONDERO,GUILHERME P.; TELÓ,GUSTAVO M.; HANSEL,DÂMARIS S.S..
ABSTRACT Tricyclazole is currently one of the fungicides recommended for the treatment of diseases in irrigated rice. However, there is relatively little information on its cytotoxic and genotoxic potential. The objective of this study was to evaluate the cytotoxicity and genotoxicity of rice crop water after apllication of the tricyclazole fungicide through the Allium cepa L. test. The rice crop water samplings were collected before and 1, 15 and 30 days after application of the fungicide in rice plant shoots. The Allium cepa roots were placed in contact with the rice crop water to check for possible chromosomal abnormalities and mitotic index of the bioindicators meristematic cells. The data obtained by the Allium cepa test indicates that the application...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioindicator; Chromosomal abnormalities; Mitotic index; Pesticide; Irrigated rice.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301251
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Cytological and cytogenic effects induced by thiabendazole on Allium cepa root meristem JBAG
Andrioli,Nancy B; Mudry,Marta D.
The toxicity and genotoxicity of thiabendazole (TBZ) in its commercial formulation Foldan® was studied in Allium cepa meristematic cells at concentrations ranging between 10 and 250 µg / ml. Our results show that exposure to TBZ induces a significant increase in the frequency of anaphase telophase chromosomal aberrations, micronuclei and binucleated cells at all the concentrations of TBZ assayed. The frequency of anaphase telophase aberrations was observed to reach a plateau at the concentration of 50 µg / ml of TBZ, which could be probably due to TBZ mitotic toxicity manifested at concentrations of 100 and 250 µg / ml. An increase was also observed in the rate of metaphase and anaphase, which was, in turn, indicative of alterations in chromatid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genotoxicity; Cytotoxicity; Mitotic index; Micronuclei; Binucleates; Thiabendazole.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332011000200002
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Cytotoxic and Genotoxic Effects of Macroptilium lathyroides Planta Daninha
SILVA,M.S.A.; YAMASHITA,O.M.; ROSSI,A.A.B.; CONCENÇO,G.; CARVALHO,M.A.C.; FELITO,R.A..
ABSTRACT: The monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 oC) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abnormalities; Bioassays; Cell division; Phasey bean; Mitotic index.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100244
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Cytotoxic and genotoxic potential of powdered juices Ciênc. Tecnol. Aliment.
BEZERRA,Marielly de Sousa; MALAQUIAS,Geiz de Sousa; CASTRO E SOUSA,João Marcelo de; PERON,Ana Paula.
Abstract Powdered juices are widely consumed by the population especially because of their convenient preparation, availability in various fruit flavors and low cost when compared to other industrialized beverages. They have complex formulation, consisting of several classes of food additives. However, there are no scientific studies on the toxicity of these foods. Thus, this study evaluated the toxicity at the cellular level of industrialized powdered juices of orange and guava flavors of three different food companies. This analysis was made using root meristem cells of Allium cepa L., at the exposure times of 24 and 48 hours, and two concentrations, 30 g/1000 mL, considered ideal for consumption according to the label of the products, and 30 g/500 mL....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Industrialized juice; Toxicity at the cellular level; Mitotic index; Cellular aberrations; Allium cepa.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000100049
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Cytotoxicity and genotoxicity of low doses of mercury chloride and methylmercury chloride on human lymphocytes in vitro BJMBR
Silva-Pereira,L.C.; Cardoso,P.C.S.; Leite,D.S.; Bahia,M.O.; Bastos,W.R.; Smith,M.A.C.; Burbano,R.R..
Mercury is a xenobiotic metal that is a highly deleterious environmental pollutant. The biotransformation of mercury chloride (HgCl2) into methylmercury chloride (CH3HgCl) in aquatic environments is well-known and humans are exposed by consumption of contaminated fish, shellfish and algae. The objective of the present study was to determine the changes induced in vitro by two mercury compounds (HgCl2 and CH3HgCl) in cultured human lymphocytes. Short-term human leukocyte cultures from 10 healthy donors (5 females and 5 males) were set-up by adding drops of whole blood in complete medium. Cultures were separately and simultaneously treated with low doses (0.1 to 1000 µg/l) of HgCl2 and CH3HgCl and incubated at 37ºC for 48 h. Genotoxicity was assessed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Methylmercury; Cytotoxicity; Genotoxicity; Mitotic index; Human lymphocytes; Chromosome aberrations.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600012
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Detection of Somaclonal Variation in Micropropagated and Acclimatized Plantlets of Oryza sativa MRQ 74 from Stem Explants Planta Daninha
SALEH,A.; TAHA,R.M.; MAHMAD,N.; ELIAS,H.; HAMID,H.A.A..
ABSTRACT: In plant tissue culture system, the excessive supply of plant growth hormones may inhibit further growth and development of the regenerants and sometimes it may induce somaclonal variation. In this study, the optimum concentration of plant growth hormones for in vitro regeneration of Oryza sativa L. cv. MRQ 74 was identified and subsequently the occurrence of somaclonal variation of regenerated plantlets was determined through cytological analysis of in vivo and in vitro grown plantlets. MS media supplemented with 0.1 mg L-1 BAP in combination with 0.1 mg L-1 NAA was found to be the optimum concentration for shoots and roots induction of Oryza sativa L. cv. MRQ 74. Plantlets derived from MS media supplemented with 0.5 mg L-1 2,4-D showed higher...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant growth hormones; Oryza sativa L. cv. MRQ 74; Survival rate; Agronomic parameters; Mitotic index; Ploidy level.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100212
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Drifter technique: a new method to obtain metaphases in Hep-2 cell line cultures BABT
Lima,Eleonidas Moura; Rissino,Jorge Dores; Guimarães,Adriana Costa; Overal,David James; Khayat,André Salim; Souza,Patrícia Carvalho de; Christofolini,Denise Maria; Smith,Marília de Arruda Cardoso; Burbano,Rommel Rodríguez.
The Hep-2 cell line is derived from laryngeal carcinoma cells and is often utilized as a model in carcinogenesis and mutagenesis tests. To evaluate the proliferative potential of this line, we developed a cytogenetic methodology (drifter technique) to obtain metaphases from cells that loose cellular adhesion when they underwent mitosis in culture. By this procedure, 2000 cells were counted, resulting in a mitotic index (MI) of 22.2%. Although this MI was not statistically different from the one obtained using either a classical cytogenetic method or a cell synchronization technique, the drifter technique has the advantage of not requiring the use of some reagents for the obtention of metaphases and also of diminishing the consumption of maintenance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hep-2 cell line; Drifter technique; Mitotic index.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500005
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In Vitro Genotoxic Effects of Four Helichrysum Species in Human Lymphocytes Cultures Biol. Res.
Erolu,Erhan H; Hamzaolu,Ergin; Aksoy,Ahmet; Budak,Ümit; Özkul,Yusuf.
Helichrysum sanguineum, Helichrysum pamphylicum, Helichrysum orientale, Helichrysum noeanum (Asteraceae) are medicinal plants. For centuries, they have been used as tea in Turkey because of their medicinal properties. So far no scientifc evidence has been found in a literature survey regarding the genotoxic effects of these plants. This work evaluated the genotoxic effects on human lymphocyte cultures induced by methanol extracts of these plants, assayed in different concentrations (0.01, 0.05, 0.1, 0.5 and 1 mg/mL). According to the results, Helichrysum noeanum, Helichrysum pamphylicum and Helichrysum sanguineum induced the formation of micronuclei and decreased the mitotic and replication indexes. Helichrysum orientale did not affect these parameters,...
Tipo: Journal article Palavras-chave: Genotoxic; Helichrysum; Micronucleus; Mitotic index; Replication index.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000200005
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Investigation on the effects of guava (Psidium guajava L.) infusions on germination, root tips and meristematic cells of Latuca sativa Anais da ABC (AABC)
Luber,Jaquelini; Palmieri,Marcel J.; Botelho,Carolina M.; Rinaldo,Daniel; Andrade-Vieira,Larissa F..
Guava (Psidium guajava L.) is a plant often employed in popular medicine. Recently several studies have alerted about the toxicity of substances present in medicinal plants, which can pose risks to the human health. In this sense, the present work aimed to investigate the phytotoxic, cytotoxic and genotoxic action of three guava varieties - Paluma, Pedro Sato and Roxa ("purple") - on the plant test system Lactuca sativa L. Thus, macro- and microscopic evaluations were carried out for five infusion concentrations (2.5, 5.0, 10.0, 20.0 and 40.0 g.L-1) prepared from each variety. Distilled water was used as negative control. Chromatographic and spectroscopic analysis by HPLC-PAD indicated that the chemical composition of the infusion of Roxa is different than...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citogenetoxicity; Root growth; Mitotic index; Seed germination; IC50.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200903
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Lack of clastogenic effects of L-thyroxine in whole-blood cultured human lymphocytes Genet. Mol. Biol.
Djelic,Ninoslav; Djelic,Dijana; Spremo-Potparevic,Biljana; Zivkovic,Lada; Markovic,Biljana; Lozance,Olivera; Blagojevic,Milos.
Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 µM to 50 µM of L-thyroxine for 24 h and 48 h, these concentrations being...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thyroxine; Human lymphocytes; Chromosome aberrations; Mitotic index; ROS.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600019
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Mitodepressive and clastogenic effects of aqueous extracts of the lichens Myelochroa lindmanii and Canoparmelia texana (Lecanorales, Parmeliaceae) on meristematic cells in plant bioassays Genet. Mol. Biol.
Campos,José Marcello Salabert de; Davide,Lisete Chamma; Soares,Geraldo Luiz Gonçalves; Viccini,Lyderson Facio.
The cytotoxicity effect of aqueous extracts of the lichens species Myelochroa lindmanii and Canoparmelia texana (Lecanorales, Parmeliaceae) were evaluated using meristematic cells of lettuce (Lactuca sativa) and maize (Zea mays). The seasonal effect was also evaluated. Extracts of M. lindmanii and C. texana inhibited root growth and/or percentage germination, possibly due to alterations in the cell cycle. The M. lindmanii extract showed anti-mitotic effects and blocked the cell cycle in metaphase so that c-mitosis and cells with chromosome duplication were produced. The C. texana extract appeared to hinder cell division, increasing the number of interphase cells. In addition, both extracts caused an increase in percentage of cell death. Clastogenic effects...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Canoparmelia texana; Cytotoxicity; Mitotic index; Myelochroa lindmanii; Plant bioassays.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100024
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Protective effect of Hemidesmus indicus R.Br. root extract against cisplatin-induced cytogenetic damage in mouse bone marrow cells Genet. Mol. Biol.
Ananthi,Rajagopal; Chandra,Nallathambi; Santhiya,Sathiyavedu T..
The aqueous extract of Hemidesmus indicus roots was investigated for its in vivo antigenotoxic effect against cisplatin-induced cytogenetic damage. Swiss albino mice were administered with various doses of the extract either singly (50, 100 and 200 mg/kg body weight) or as split doses (10, 20 and 40 mg/kg bw/day) for five consecutive days by oral gavage. As endpoints, chromosome aberrations, micronuclei in polychromatic erythrocytes, mitotic index and PCE/NCE ratio were estimated. The extract protected the bone marrow cells from cisplatin-induced genotoxicity in an inverse dose-dependent manner. However, the extract was cytotoxic at all doses. But, under split dose regime it conferred a higher level of genoprotection and was not cytotoxic at the lower two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genoprotective; Cytotoxic; Chromosomal aberrations; Micronucleus; Mitotic index.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100030
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Role of p53 codon 72 polymorphism in chromosomal aberrations and mitotic index in patients with chronic hepatitis B BJMBR
Akbaş,H.; Yalcin,K.; Isi,H.; Tekes,S.; Atay,A.E.; Akkus,Z.; Budak,T..
Polymorphisms of the p53 gene, which participates in DNA repair, can affect the functioning of the p53 protein. The Arg and Pro variants in p53 codon 72 were shown to have different regulation properties of p53-dependent DNA repair target genes that can affect various levels of cytogenetic aberrations in chronic hepatitis B patients. The present study aimed to examine the frequency of chromosomal aberrations and the mitotic index in patients with chronic hepatitis B and their possible association with p53 gene exon 4 codon 72 Arg72Pro (Ex4+119 G>C; rs1042522) polymorphism. Fifty-eight patients with chronic hepatitis B and 30 healthy individuals were genotyped in terms of the p53 gene codon 72 Arg72Pro polymorphism by PCR-RFLP. A 72-h cell culture was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepatitis B; Chromosomal aberrations; Mitotic index; P53 gene codon 72 polymorphism.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100003
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The cytogenetic effects of black tea and green tea on cultured human lymphocytes BABT
Eroğlu,Halil Erhan.
In this study, the cytogenetic effects of black tea and green tea were determined in cultured peripheral blood lymphocytes. Results showed that black tea and green tea induced the mitotic and replication indexes and decreased micronuclei. But these data were not statistically significant for green tea. The effects of black tea on the micronucleus formation and mitotic index were statistically significant. The decrease in micronucleus counts indicated that black tea and green tea had considerable anticlastogenic and antigenotoxic effects as observed in vitro in human lymphocytes. Thus, it could be concluded that tea polyphenols protected the normal cells from genotoxic or carcinogenic agents, which indicated the therapeutic and antioxidative role of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tea; Catechins; Micronucleus; Mitotic index; Replication index.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600011
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