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Registros recuperados: 10
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Assessment of the degree of contamination of rat germ cell preparations using specific cDNA probes BJMBR
Recent reports showing a decrease in sperm count in men have brought new concerns about male infertility. Animal models have been widely used to provide some relevant information about the human male gamete, and extrapolations are made to men and to the clinical context. The present study assesses one of the methods used for separation of germ cells of the adult rat testis, namely centrifugal elutriation followed by density gradients (Percoll®). This method was chosen since it presents the best results for cell purity in separating germ cells from the rat testis. A comparison between continuous and discontinuous Percoll® gradients was performed in order to identify the best type of gradient to separate the cells. Maximal cell purity was obtained for...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Rat testis; Germ cell separation; Percoll; Molecular analysis; Transition protein 2 (TP2); Mitochondrial cytochrome C oxidase II (COX II); Sulfated glycoprotein 1 (SGP1).
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300007
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Detection of Bonamia ostreae and B. exitiosa (Haplosporidia) in Ostrea edulis from the Adriatic Sea (Italy) ArchiMer
Nardsi, V.; Arzul, Isabelle; Cargini, D.; Mosca, F.; Calzetta, A.; Traversa, D.; Robert, Maeva; Joly, Jean-pierre; Chollet, Bruno; Renault, Tristan; Tiscar, P. G..
The flat oyster Ostrea edulis L. is widespread along the Italian coasts. In particular, the Manfredonia Gulf (Adriatic Sea) represents an important site where natural beds subsist. Previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of Bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of O. edulis was carried out at this location in 2007. Of 750 oysters collected, 3 showed the presence of uninucleated microcells (2 to 3 mu m diameter) free or inside the haemocyte cytoplasm by cytology and histopathology. Molecular analysis confirmed that the microcells in 2 oysters were B. exitiosa, whereas in the third oyster the microcells were B. ostreae. Moreover,...
Tipo: Text Palavras-chave: Bonamia spp.; Ostrea edulis; Molecular analysis; Electron microscopy.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11284/7825.pdf
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Determination of the mode of reproduction of bahiagrass hybrids using cytoembryological analysis and molecular markers R. Bras. Zootec.
Weiler,Roberto Luis; Dall’Agnol,Miguel; Simioni,Carine; Krycki,Karine Cristina; Dahmer,Nair; Guerra,Divanilde.
ABSTRACT The aim of this study was to determine the mode of reproduction of a hybrid progeny derived from intraspecific crosses of Paspalum notatum through cytoembryological analysis and use of RAPD (random amplification of polymorphic DNA) molecular markers. Cytoembryological analysis allowed identification of the mode of reproduction of 28 plants that were selected after agronomic productivity evaluations. Of these, 19 had embryo sac morphology compatible with an apomictic mode of reproduction and nine had embryo sac morphology compatible with a sexual mode of reproduction. Meanwhile, molecular marker analysis for 194 individuals showed 54 sexual and 140 apomictic plants; of the 28 plants analyzed by the two methods, ten results (35.7%) were in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apomixis; Molecular analysis; Paspalum notatum; Plant breeding; RAPD.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000300185
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Does the Glyphosate Treatment Interfere Negatively on RNA Integrity in Glyphosate-Resistant and -Sensitive Conyza bonariensis? Planta Daninha
PIASECKI,C.; BENEMANN,D.; CARVALHO,I.R.; AGOSTINETTO,D.; STEWART Jr.,C.N.; VARGAS,L..
ABSTRACT: The hairy fleabane ( Conyza bonariensis (L.) Cronq.) is among the most problematic glyphosate-resistant weeds to manage around the world. In weed science, molecular approaches such as RNA sequencing (RNA-Seq) and quantitative reverse-transcription polymerase chain reaction (RT-qPCR) have been employed to study molecular responses to glyphosate treatment in Conyza species. Glyphosate treatment leads to reactive oxygen species (ROS) production in plants which could damage the RNA. Degraded RNA is an issue and can compromise further molecular analysis. Thus, the objective of this study was to evaluate whether glyphosate treatment interferes negatively on RNA integrity of glyphosate-resistant and -sensitive hairy fleabane biotypes. Two experiments...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hairy fleabane; Molecular analysis; RNA integrity number (RIN).
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100175
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Molecular Analysis of Spinal Muscular Atrophy: a genotyping protocol based on TaqMan® real-time PCR Genet. Mol. Biol.
Godinho,Fernanda Marques de Souza; Bock,Hugo; Gheno,Tailise Conte; Saraiva-Pereira,Maria Luiza.
Spinal muscular atrophy (SMA) is an autosomal recessive inherited disorder caused by alterations in the survival motor neuron I (SMN1) gene. SMA patients are classified as type I-IV based on severity of symptoms and age of onset. About 95% of SMA cases are caused by the homozygous absence of SMN1 due to gene deletion or conversion into SMN2. PCR-based methods have been widely used in genetic testing for SMA. In this work, we introduce a new approach based on TaqMan® real-time PCR for research and diagnostic settings. DNA samples from 100 individuals with clinical signs and symptoms suggestive of SMA were analyzed. Mutant DNA samples as well as controls were confirmed by DNA sequencing. We detected 58 SMA cases (58.0%) by showing deletion of SMN1 exon 7....
Tipo: Info:eu-repo/semantics/article Palavras-chave: SMA; SMN1 gene; Gene conversion; Molecular analysis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600010
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Molecular basis of high-level ciprofloxacin resistance in Neisseria gonorrhoeae strains from Shandong Province, China BJM
Zhao,L.H.; Zhao,S.P..
In the study, the ciprofloxacin resistance rate was 100%. High-level ciprofloxacin resistance rate was 63.55%. Sixteen different mutation patterns involved in the formation of ciprofloxacin resistance were identified. The most prevalent were patterns P7 (25.2%), P8 (15.0%), P9 (11.2%), P1 (10.3%), and P5 (10.3%). All of the 107 NG isolates analyzed for mutations in the study have demonstrated a change of Ser-91 → Phe in the gyrA gene, and all except one have demonstrated a change in position 95 of the amino acid sequence. All of the 68 high-level QRNG isolates had double mutations in gyrA gene combined with a single or two mutations in parC gene. It is most important that a new mutation site of Ile-97 → Met in gyrA and a new mutation of Leu-106 → Ile in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neisseria gonorrhoeae; Ciprofloxacin resistance; High-level; Molecular analysis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100039
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Morphological and molecular identification of four Brazilian commercial isolates of Pleurotus spp. and cultivation on corncob BABT
Menolli Junior,Nelson; Asai,Tatiane; Capelari,Marina; Paccola-Meirelles,Luzia Doretto.
The species of Pleurotus have great commercial importance and adaptability for growth and fructification within a wide variety of agro-industrial lignocellulosic wastes. In this study, two substrates prepared from ground corncobs supplemented with rice bran and charcoal were tested for mycelium growth kinetics in test tubes and for the cultivation of four Pleurotus commercial isolates in polypropylene bags. The identification of the isolates was based on the morphology of the basidiomata obtained and on sequencing of the LSU rDNA gene. Three isolates were identified as P. ostreatus, and one was identified as P. djamor. All isolates had better in-depth mycelium development in the charcoal-supplemented substrate. In the cultivation experiment, the isolates...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Charcoal; Edible mushroom cultivation; Molecular analysis; Taxonomy.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200019
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Potential use of molecular-typing methods for the identification and characterization of salmonella enterica serotypes isolated in the poultry production chain Rev. Bras. Ciênc. Avic.
Baratto,CM; Gelinski,JMLN; Bernardi,AZ; Marafon,A; Braun,F.
Salmonella is widespread in nature and can be found in all links of the poultry production chain. Due to its high impact on meat processing, techniques for the rapid detection and reproducible characterization of Salmonella serotypes in foods are needed. The present study investigated the potential of molecular profiling to identify and differentiate 15 Salmonella serotypes isolated from the poultry production chain, based on 5 primers by random amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus (ERIC-PCR), amplification of rDNA internal spacer analysis (RISA), and amplified ribosomal DNA restriction analysis (ARDRA) of 16S-23S rRNA internal spacer region (ISR) cleaved with Alu I and Hha I restriction enzymes. Three isolates...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular analysis; Salmonella; Serotypes; Typing.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000300003
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Quantitative analysis of Terminal Restriction Fragment Length Polymorphism (T-RFLP) microbial community profiles: peak height data showed to be more reproducible than peak area BJM
Caffaro-Filho,Roberto A.; Fantinatti-Garboggini,Fabiana; Durrant,Lucia R..
Terminal Restriction Fragment Length Polymorphism (T-RFLP) is a culture-independent fingerprinting method for microbial community analysis. Profiles generated by an automated electrophoresis system can be analysed quantitatively using either peak height or peak area data. Statistical testing demontrated that peak height data showed to be more reproducible than peak area data.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Terminal Restriction Fragment Length Polymorphism (T-RFLP); Molecular analysis; Microbial community.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400027
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Updating Geographic Distribution of Artemia urmiana GÜNTHER, 1890 (Branchiopoda: Anostraca) in Europe: An Integrated and Interdisciplinary Approach IBSS Repository
Abatzopoulos, Th. J.; Amat, F.; Baxevanis, A. D.; Belmonte, G.; Hontoria, F.; Maniatsi, S.; Moscatello, S.; Mura, G.; Shadrin, N. V..
Artemia urmiana (a species previously considered endemic of Lake Urmia, NW Iran) has been found in Lake Koyashskoe, a hypersaline lake on the Black Sea coast of the Crimean peninsula (Ukraine). Therefore, this is the first record of A. urmiana in Europe which updates its distribution. The species identification was based on an integrated and interdisciplinary approach using discriminant analysis of the morphometric characters, scanning electron microscopy, and molecular profile analysis. The data derived from the above mentioned approaches converge to significant similarity of the population under investigation with A. urmiana. The updated geographic distribution of the species, deriving from the present report, asks for additional contribution of other...
Tipo: Journal Contribution Palavras-chave: Salt lakes; Scanning electron microscopy; Discriminant analysis; Molecular analysis; Biogeography.
Ano: 2009 URL: http://repository.ibss.org.ua/dspace/handle/99011/1069
Registros recuperados: 10
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