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Registros recuperados: 54
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A biologia avançada e o impacto da genômica na produção de caprinos e ovinos. Infoteca-e
SIDER, L. H.; ZAROS, L. G..
bitstream/CNPC-2010/21844/1/doc78.pdf
Tipo: Documentos (INFOTECA-E) Palavras-chave: Genômica; Caprino; Ovino; Biologia molecular; Goats; Sheep; Molecular biology; Genomics.
Ano: 2008 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/534121
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A role in sugar and redox handling of maize aldose reductase. Repositório Alice
SOUSA, S. M. de; KIYOTA, E.; YUNES, J. A.; KOCH, K. E..
Tipo: Parte de livro Palavras-chave: Biologia molecular; Molecular biology.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/899387
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An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran BJID
Farahmand,Mahin; Nahrevanian,Hossein; Shirazi,Hasti Atashi; Naeimi,Sabah; Farzanehnejad,Zahra.
Cutaneous leishmaniasis (CL) is a widespread tropical infection which has a high incidence rate in Iran. Leishmania tropica, the causative agent of anthroponotic cutaneous leishmaniasis (ACL), and Leishmania major, which causes zoonotic cutaneous leishmaniasis (ZCL), are endemic in various parts of Iran with a high incidence rate. The aim of this study was to evaluate the reappraisal of the diagnosis and epidemiology of CL in Iran, by different clinical, parasitological and molecular assays among patients suspected of CL referred to the Department of Parasitology, at the Pasteur Institute of Iran during 2006-2009. Two hundred samples from patients with ulcerative skin lesions were collected, clinical analyses were applied, data questionnaire was completed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parasitological analysis; Molecular biology; Leishmaniasis; Cutaneous; Iran; Epidemiology.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100004
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Aplicação da biologia molecular em programas de conservação de recursos pesqueiros. Infoteca-e
MARQUES, D. K. S..
Estudos genéticos em peixes; Marcadores moleculares; Eletroforese de proteínas; Seqüências de DNA como marcadores; Polimorfismo de comprimento de fragmentos de restrição (Restriction Fragment Length Polymorphism - RFLP); Polimorfismo de DNA amplificado ao acaso (Randomly Aplified polymorphic DNA - RAPD); DNA com seqüências repetitivas: micro-satélites e mini-satélites; DNA mitocondrial.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Marcadores moleculares; Recursos pesqueiros; Fisheries resources; Conservation.; Biologia Molecular; Conservação; Peixe; População.; Fish; Molecular biology; Population..
Ano: 2002 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/810724
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Applications of nucleoside-based molecular probes for the in vivo assessment of tumour biochemistry using positron emission tomography (PET) BABT
Wiebe,Leonard I..
Positron emission tomography (PET) is a non-invasive nuclear imaging technique. In PET, radiolabelled molecules decay by positron emission. The gamma rays resulting from positron annihilation are detected in coincidence and mapped to produce three dimensional images of radiotracer distribution in the body. Molecular imaging with PET refers to the use of positron-emitting biomolecules that are highly specific substrates for target enzymes, transport proteins or receptor proteins. Molecular imaging with PET produces spatial and temporal maps of the target-related processes. Molecular imaging is an important analytical tool in diagnostic medical imaging, therapy monitoring and the development of new drugs. Molecular imaging has its roots in molecular biology....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular biology; Molecular imaging; PET; Nucleosides; Nucleotides; Nucleic acids; Oligonucleotides; Antisense; Aptamer; Spiegelmer.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300011
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Assessment of phenotypic indexes for aluminum tolerance in maize using nutrient solution. Repositório Alice
CANCADO. G. M. A.; MARTINS, P. R.; PARENTONI, S. N.; OLIVEIRA, A. B.; LOPES, M. A..
Tipo: Parte de livro Palavras-chave: Tolerancia; RFLP; Aluminium; Tolerance; Savanna.; Alumínio; Biologia Molecular; Cerrado.; Molecular biology; Soil..
Ano: 1999 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/483530
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Assessment of the scientific-technological production in molecular biology in Brazil (1996-2007): the contribution of genomics programs Anais da ABC (AABC)
Meneghini,Rogério; Gamba,Estêvão C..
Several genome sequencing programs were launched in Brazil by the end of the nineties and the early 2000s.The most important initiatives were supported by the ONSA program (http://watson.fapesp.br/onsa/Genoma3.htm) and aimed at gaining domain in genomic technology and bringing molecular biology to the state of art. Two mainsets of data were collected in the 1996-2007 period to evaluate the results of these genome programs: the scientific production (Scopus and Web of Science databases) and the register of patents (US Patent and Trademark Office), both related to the progress of molecular biology along this period. In regard to the former, Brazil took a great leap in comparison to 17 other developed and developing countries, being only surpassed by China....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Science assessment; Genomics; Scientometrics; Molecular biology.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200031
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Biochemistry and molecular biology of N uptake and assimilation by plants. Repositório Alice
PURCINO, A. A. C.; ALVES, V. M. C.; PARENTONI, S. N.; MARRIEL, I. E.; SANTOS, F. G. dos; FRANCA, G. E..
Tipo: Parte de livro Palavras-chave: Biologia Molecular; Bioquímica; Nitrogênio.; Biochemistry; Molecular biology; Nitrogen..
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/480862
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Biologia molecular aplicada à diagnose de doenças de plantas. Infoteca-e
GONCALVES, R. C..
As doenças das plantas influenciam negativamente e de modo significativo as atividades agropecuárias e florestais em todo o mundo. Muitas epidemias tiveram como conseqüência grandes perdas econômicas, alterações sociais e ambientais.
Tipo: Capítulo em livro técnico (INFOTECA-E) Palavras-chave: Marcadores genéticos.; Enfermedades y desórdenes de las plantas; Doença de planta; Marcador genético; Biologia Molecular.; Plant diseases and disorders; Molecular biology; Genetic markers..
Ano: 2009 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/661798
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Biologia molecular aplicada à sanidade. Infoteca-e
SIDER, L. H..
bitstream/item/48454/1/Midia-Biologia-molecular.pdf
Tipo: Artigo de divulgação na mídia (INFOTECA-E) Palavras-chave: Biologia molecuar; Sanidade; Molecular biology.
Ano: 2009 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/577884
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Caracterização através da PCR de cepas de Bacillus thuringiensis de diferentes regiões do Brasil. Repositório Alice
VALICENTE, F. H.; VASCONCELOS, M. J. V.; PAIVA, E.; FARIA, A. L.; SOUZA, F. D.; BARRETO, M. R..
Tipo: Parte de livro Palavras-chave: Brasil.; Biologia Molecular; Bacillus Thuringiensis; Solo.; Brazil; Molecular biology; Soil..
Ano: 1998 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/482991
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Carbapenem and cefoxitin resistance of Klebsiella pneumoniae strains associated with porin OmpK36 loss and DHA-1 β-lactamase production BJM
Shi,Weifeng; Li,Kun; Ji,Yun; Jiang,Qinbo; Wang,Yuyue; Shi,Mei; Mi,Zuhuang.
Clinical isolates of carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae) strains are being increased worldwide. Five pan-resistant K. pneumoniae strains have been isolated from respiratory and ICU wards in a Chinese hospital, and reveal strong resistance to all β-lactams, fluoroquinolones and aminoglycosides. Totally 27 β-lactamase genes and 2 membrane pore protein (porin) genes in 5 K. pneumoniae strains were screened by polymerase chain reaction (PCR). The results indicated that all of 5 K. pneumoniae strains carried blaTEM-1 and blaDHA-1 genes, as well as base deletion and mutation of OmpK35 or OmpK36 genes. Compared with carbapenem-sensitive isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the resistant isolates...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; AmpC β-Lactamase; Porin; Molecular biology.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200015
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Caryocar brasiliense camb protects against genomic and oxidative damage in urethane-induced lung carcinogenesis BJMBR
Colombo,N.B.R.; Rangel,M.P.; Martins,V.; Hage,M.; Gelain,D.P.; Barbeiro,D.F.; Grisolia,C.K.; Parra,E.R.; Capelozzi,V.L..
The antioxidant effects of Caryocar brasiliense Camb, commonly known as the pequi fruit, have not been evaluated to determine their protective effects against oxidative damage in lung carcinogenesis. In the present study, we evaluated the role of pequi fruit against urethane-induced DNA damage and oxidative stress in forty 8-12 week old male BALB/C mice. An in vivo comet assay was performed to assess DNA damage in lung tissues and changes in lipid peroxidation and redox cycle antioxidants were monitored for oxidative stress. Prior supplementation with pequi oil or its extract (15 µL, 60 days) significantly reduced urethane-induced oxidative stress. A protective effect against DNA damage was associated with the modulation of lipid peroxidation and low...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pequi; Caryocar brasiliense Camb; Oxidative stress; Lung cancer; Urethane; Molecular biology.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900852
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Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil PAB
Calegario,Renata Faier; Ferreira,Sávio de Siqueira; Andrade,Eduardo Chumbinho de; Zerbini,Francisco Murilo.
The objective of this work was the biological and molecular characterization of a begomovirus detected in São Joaquim de Bicas, Minas Gerais, Brazil, named TGV-[Bi2], by determining its host range, complete nucleotide sequence and phylogenetic relationships with other begomoviruses. Biological characterization consisted of a host range study using either sap inoculation or particle bombardment as inoculation methods. The yellow spot virus can infect plants in Solanaceae and Amaranthaceae, including economically importat crops as sweet pepper, and weeds as Datura stramonium and Nicotiana silvestris. For the molecular characterization, the full-length genome (DNA-A and DNA-B) was amplified, cloned and completely sequenced. Sequence comparisons and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; Bemisia tabaci; ToYSV; Molecular biology; Geminivirus; Whitefly.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000900016
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Computational investigations in eukaryotes genome de novo assembly using short reads. Repositório Alice
CINTRA, L. C..
Recently news technologies in molecular biology enormously improved the sequencing data production, making it possible to generate billions of short reads totalizing gibabases of data per experiment. Prices for sequencing are decreasing rapidly and experiments that were impossible in the past because of costs are now being executed. Computational methodologies that were successfully used to solve the genome assembler problem with data obtained by the shotgun strategy, are now inefficient. Efforts are under way to develop new programs. At this moment, a stabilized condition for producing quality assembles is to use paired-end reads to virtually increase the length of reads, but there is a lot of controversy in other points. The works described in literature...
Tipo: Anais e Proceedings de eventos Palavras-chave: Genomas de eucariotos; Bioinformática.; Biologia Molecular; Genoma.; Genome; Eukaryotic cells; Molecular biology; Bioinformatics.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/908741
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Contribuição à filogenia de abelhas Melipona com uso de sequências parciais da região ITS1 do nrDNA. Repositório Alice
BOMFIM, I. G. A.; FREITAS, B. M.; VENTURIERI, G. C.; JORGE, D. M. de M.; LOBO, M. D. P.; TORRES, D. C.; GRANGEIRO, T. B..
O objetivo deste estudo foi investigar, por meio de dados moleculares de sequências de DNA, as relações filogenéticasde abelhas indígenas sem ferrão do táxon Melipona nativas do Brasil. Procurou-se, assim, fornecersubsídios para facilitar uma futura revisão taxonômica sobre esse grupo de abelhas. Sequências parciais daregião do espaçador transcrito interno I (ITS1) do DNA ribossômico nuclear (nrDNA) foram obtidas de espécimesde M. flavolineata, M. mondury e M. fasciculata, coletados em estados do Norte, Nordeste e Sudestedo Brasil. O alinhamento múltiplo das sequências geradas em conjunto com outras 25 do gênero Melipona,depositadas no GenBank ou publicadas em artigos científicos por outros autores, apresentou 141 caracteresalinhados, com uma distância...
Tipo: Separatas Palavras-chave: Sequenciamento automático; Reconstrução filogenética; Abelhas sem ferrão; Meliponicultura; Automated sequencing; Phylogenetic reconstruction.; Biologia Molecular.; Molecular biology; Stingless bees..
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1017063
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Cytogenetic and molecular analyses of Oryza sativa L. x O. australiensis Domin. and O. sativa L. x O. brachyantha A. Chev. et Roehr derivatives International Rice Research Institute
Abbasi, Fida M..
xv, 119 leaves : ill. Thesis (Ph.D.) -- University of the Philippines at Los Baños
Tipo: Thesis Palavras-chave: Rice; Cytogenetics; Hybrids; Wild rice; Plant breeding; Molecular biology.
Ano: 1999 URL: http://hdl.handle.net/123456789/536
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Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands Arq. Bras. Med. Vet. Zootec.
Souza,D.M.B.; Barros,M.G.O.; Silva,J.S.C.; Silva,M.B.; Coleto,Z.F.; Jimenez,G.C.; Adrião,M.; Wischral,A..
Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bitch; DNA; Mamma; Molecular biology; Neoplasm; PCR.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013
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Detection of Mycobacterium avium subsp. paratuberculosis in bovine milk from the state of Pernambuco, Brazil BJM
Albuquerque,Pedro Paulo Feitosa de; Santos,André de Souza; Souza Neto,Orestes Luiz de; Kim,Pomy de Cássia Peixoto; Cavalcanti,Erika Fernanda Torres Samico Fernandes; Oliveira,Júnior Mário Baltazar de; Mota,Rinaldo Aparecido; Pinheiro Júnior,José Wilton.
Abstract The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular biology; Diagnosis; Paratuberculosis; Milk.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100113
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Déterminismes génétiques et écophysiologiques du fonctionnement des légumineuses : Conséquences sur la dynamique d’élaboration du rendement et de la qualité National Institute of Agronomic Research
Munier-Jolain, Nathalie; Salon, Christophe.
L'unité de recherche Génétique et Amélioration des plantes (URGAP DIJ) a comme axe prioritaire de recherche la qualité de la graine de légumineuse. Ses études portent sur le pois protéagineux (valeur nutritionnelle de la graine en alimentation animale, nutrition azotée de la plante) et s'appuient sur des espèces modèles dont notamment Medicago truncatula. Elles font appel aux méthodologies de la biologie moléculaire, cellulaire, de la culture in vitro, de l'expérimentation en serre et au champ.
Tipo: Working Paper Palavras-chave: Corn; Molecular biology; Céréale; Biologie moléculaire; Génétique; Valeur nutritive.
Ano: 2004 URL: http://hdl.handle.net/2174/86
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