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Registros recuperados: 27
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A new PCR approach for the identification of Fusarium graminearum BJM
Biazio,Gleison Ricardo de; Leite,Gabriela Guimarães Sousa; Tessmann,Dauri José; Barbosa-Tessmann,Ione Parra.
The main objective of this work was to develop a PCR protocol for the identification of Fusarium graminearum, based on a pair of primers targeted to a segment of the 3' coding region of the gaoA gene that codes for the enzyme galactose oxidase (GO). This region has low homology with the same region of GO genes from other fungi. Genomic DNA from 17 strains of Fusarium spp. isolated from diseased cereals, from several other Fusarium species, and from other fungi genera was analyzed in a PCR assay using this primer set. The 17 strains of Fusarium spp. were also analyzed for the GO enzyme production in submerse fermentation in a new formulated liquid medium. All strains that were morphologically and molecularly identified as F. graminearum were able to secrete...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium graminearum; Galactose oxidase; GaoA gene; Molecular identification.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300028
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An overview on molecular markers for detection of ochratoxigenic fungi in coffee neans BABT
Fungaro,Maria Helena Pelegrinelli; Sartori,Daniele.
Ochratoxin A (OTA) is one of the most abundant food-contaminating mycotoxins. Its occurrence in several agricultural commodities has been considered a worldwide problem. This toxin is mainly produced by Aspergillus species. OTA has nephrotoxic, immunosuppressive, and carcinogenic effects and consequently the contamination with this toxin represents a high risk for human and animal health. In the last 5 years, several investigators have applied molecular methods in order to develop PCR assays for identifying and quantifying OTA-producing fungi in coffee beans samples. The main objective is to allow the detection of microorganisms capable of producing OTA, preferentially prior to ochratoxin production and accumulation. In this contribution several of these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ochratoxigenic fungi; Molecular marker; PCR detection; Molecular identification.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700001
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Characterization of Ectomycorrhizal species through molecular biology tools and morphotyping Scientia Agricola
Clasen,Bárbara Estevão; Silveira,Andressa de Oliveira; Baldoni,Daiana Bortoluzzi; Montagner,Daiane Fiuza; Jacques,Rodrigo Josemar Seminoti; Antoniolli,Zaida Inês.
ABSTRACT: Mycorrhizae are mutualistic associations between fungi and plant roots. These symbiotic associations are abundant and occur in 75 to 80 % of plants. Ectomycorrhizal fungi are very important in ecosystems, because their mutualistic association with plants of different species helps nutrients and water absorption, as well as protection of the host plant against pathogens and abiotic stresses. Most ectomycorrhizal fungi belong to the Basidiomycota class, such as the following genera: Amanita, Hebeloma, Hysterangium, Laccaria, Lactarius, Rhizopogon, Russula, Scleroderma, Suillus, Tricholoma, among others. Morphological studies on ectomycorrhizae report important results in understanding the species biodiversity. However, the use of molecular biology...
Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR; Ectomycorrhizal fungi; Molecular identification; Morphotype.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000300246
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Characterization of Some Efficient Cellulase Producing Bacteria Isolated from Pulp and Paper Mill Effluent Contaminated Soil BABT
Reddy,Kondakindi Venkateswar; Vijayalashmi,Tatiparti; Ranjit,Pabbati; Raju,Maddela Naga.
ABSTRACT The wide variety of bacteria in the environment permits screening for more efficient cellulases to help overcome current challenges in biofuels production. This study focuses on the isolation of efficient cellulase producing bacteria found in pulp and paper mill effluent contaminated soil which can be considered for use in large scale biorefining. Four different bacterial strains were isolated and screened for cellulase production by using CMC agar medium. All isolates showed cellulase activity these strains were further characterized by morphological, physiological, biochemical and 16S rRNA gene analyses. These isolates were identified as two Bacillus subtilis sub sps, Bacillus mojavensis and Bacillus cereus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: CMC Agar; Cellulolytic Bacteria; Molecular identification; 16S rRNA; Cellulolytic activity.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100442
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Culture and molecular identification of microorganisms from Digital Dermatitis lesions in dairy cattle: Leptospira, an unexpected finding Arq. Bras. Med. Vet. Zootec.
Diniz,S.; Sandes,S.H.C.; Bomfim,M.R.Q.; Santos,P.C.; Cruz,F.D.; Moreira,T.F.; Carvalho,M.R.S.; Cisalpino,P.S.; Moreira,E.S. A..
ABSTRACT Bovine digital dermatitis (BDD) is an infectious and contagious disease characterized by ulcerative and proliferative lesions affecting the skin on the bulbs of the heel or the interdigital cleft in dairy cattle, often associated with lameness. Evidences on the etiology of BDD indicate that it is multifactorial, involving environmental factors and multiple bacterial colonization. We isolated and identified microorganisms from BDD biopsy samples obtained from five Holstein Friesian and two Jersey cows by cultivation and molecular identification of bacterial isolates using 16S rRNA gene sequence analysis. We identified six bacterial species: Spirochetes as Treponema pedis and Leptospira broomi/L. fainei, L. licerasiae/L. wolffii; Corynebacterium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine digital dermatitis; Molecular identification; 16S rRNA gene analysis; Treponema; Leptospira.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300559
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Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples Anais da ABC (AABC)
PIE,MARCIO R.; STRÖHER,PATRÍCIA R.; AGOSTINIS,ANDRÉ O.; BELMONTE-LOPES,RICARDO; TADRA-SFEIR,MICHELLE Z.; OSTRENSKY,ANTONIO.
ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: EDNA; Invasive species; Environmental DNA; Molecular identification.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301041
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Development of sequence characterized amplified region markers from intersimple sequence repeat fingerprints for the molecular detection of toxic phytoplankton Alexandrium catenella (Dinophyceae) and pseudo-Nitzschia pseudodelicatissima (Bacillariophyceae) from French coastal waters ArchiMer
Bornet, Benjamin; Antoine, Elisabeth; Francoise, Sylvaine; Marcaillou-le Baut, Claire.
Harmful algal blooms are a serious threat to shellfish farming and human health all over the world. The monitoring of harmful algae in coastal waters originally involved morphological identification through microscopic examinations, which was often difficult unless performed by specialists and even then often did not permit identification of toxic species. More recently, specific molecular markers have been used to identify specific phytoplankton species or strains. Here we report on the use of the intersimple sequence repeat (ISSR) technique to develop specific sequence characterized amplified region markers (SCAR) and to identify with these tools two toxic species in French coastal waters, the diatom Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and...
Tipo: Text Palavras-chave: SCAR; Pseudo nitzschia; Monitoring; Molecular identification; ISSR; Alexandrium.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-780.pdf
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Diversity of yeasts during fermentation of cocoa from two sites in the Brazilian Amazon Acta Amazonica
ALMEIDA,Silvana de F. Oliveira de; SILVA,Letícia R Carvalho; JUNIOR,Gilson Celso A. Chagas; OLIVEIRA,Guilherme; SILVA,Silvia Helena Marques da; VASCONCELOS,Santelmo; LOPES,Alessandra Santos.
ABSTRACT The purpose of this study was to identify the yeasts involved in spontaneous fermentation of cocoa from the Brazilian Amazon region. The fermentation process was carried out experimentally with cocoa seeds from two sites (Medicilândia and Tucumã), State of Pará, northern Brazil, during a six-day period. Totals of 44 yeasts were isolated from Medicilândia and 29 from Tucumã. Molecular identification was carried out by sequencing the D1/D2 region fragment of the rRNA 26S gene, expanded with universal primers for the NL1GC and LS2 eukaryotes. Pichia manshurica and Saccharomyces cerevisiae were identified in Medicilândia and five yeast species (Pichia fermentans, P. kudriavzevii, P. manshurica, S. cerevisiae and Zygosaccharomyces bailii) were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Accession; Molecular identification; Cocoa beans; Pichia Manshurica; Saccharomyces cerevisiae; Theobroma cacao.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000100064
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Dominant yeasts associated to mango (Mangifera indica) and rose apple (Syzygium malaccense) fruit pulps investigated by culture-based methods Anais da ABC (AABC)
RAMÍREZ-CASTRILLÓN,MAURICIO; USMAN,LUISA M.; SILVA-BEDOYA,LINA M.; OSORIO-CADAVID,ESTEBAN.
Abstract: The biotechnological potential of yeasts associated to different habitats in Colombia has been poorly studied, especially the yeasts associated with different plant structures. Fruit pulps are interesting substrates mainly for the growth of yeast species, that can positively affect the productivity and quality of some bioeconomic species. Therefore, the objective of this study was to identify the dominant yeast species associated with mango and rose apple fruit pulps in Cali, Colombia. A total of 90 isolates were obtained, which were grouped considering their colony morphology. The D1/D2 domain of the large ribosomal RNA gene (LSU rRNA gene) or internal transcribed spacer (ITS) 1, ribosomal gene 5.8S and ITS 2 (ITS) regions of one to several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mangifera indica; Syzygium malaccense; Molecular identification; Yeasts; Colombia.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700626
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Efficient detection of Frankliniella schultzei (Thysanoptera, Thripidae) by cytochrome oxidase I gene (mtCOI) direct sequencing and real-time PCR BABT
Leão,Evelynne Urzêdo; Spadotti,David Marques de Almeida; Rocha,Kelly Cristina G.; Lima,Élison Fabricio B.; Tavella,Luciana; Turina,Massimo; Krause-Sakate,Renate.
ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular identification; Real-time PCR; Tospovirus vector.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100454
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Evaluation of the genetic diversity among maize lines (Zea mays L.) by DNA fingerprinting analysis Ciência Rural
Echeverrigaray,Sergio; Delamare,Ana Paula Longaray; Tosello,Geraldo; Gal,Orit; Hillel,Jossi; Lavi,Uri.
Informativo DNA fingerprint profiles of eight homozygotic maize lines were obtained by the electrophoretic separation of DNA restriction fragments and the ir hybridization with the minisatellite probe R18.1. The analysis of the bandsharing frequencies allowed to identify all the lines and to estimate the genetic distances between them. The relationship obtained by DNA fingerprinting analysis of the eight inbreed lines was highly consistem with the ir genetical origin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular identification; Genetic distances; Zea mays.
Ano: 1996 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000300028
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Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential. Repositório Alice
MAIA, N. da C.; SOUZA, P. N. da C.; GODINHO, B. T. V.; MOREIRA, S. I.; ABREU, L. M. de; JANK, L.; CARDOSO, P. G..
This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi inhibited...
Tipo: Artigo de periódico Palavras-chave: Antagonism; Molecular identification; RDNA; Grasses; Plant pathogens.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110371
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Fungal endophytes of Panicum maximum and Pennisetum purpureum: isolation, identification, and determination of antifungal potential R. Bras. Zootec.
Maia,Natália da Costa; Souza,Patrícia Nirlane da Costa; Godinho,Bárbara Temponi Vilarino; Moreira,Silvino Intra; Abreu,Lucas Magalhães de; Jank,Liana; Cardoso,Patrícia Gomes.
ABSTRACT This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antagonism; Grasses; Molecular identification; Plant pathogens; RDNA.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100303
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Genetic diversity of the root-knot nematode Meloidogyne enterolobii and development of a SCAR marker for this guava-damaging species. Repositório Alice
TIGANO, M. S.; SIQUEIRA, K. de; CASTAGNONE-SERENO, P.; MULET, K.; QUEIROZ, P. R.; SANTOS, M. DOS; TEIXEIRA, C.; ALMEIDA, M. R. A.; CARNEIRO, R. M. D. G..
Tipo: Separatas Palavras-chave: Molecular identification; RAPD; ISSR; Psidium Guajava.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/872608
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Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul1. Repositório Alice
CAZOLA, D de O.; JORGE, K. dos S. G.; ZUMÁRRAGA, M. J.; SOUZA-FILHO, A. F.; ARAUJO, F. R.; OSÓRIO, A. L. A. R..
Neste estudo, realizou-se genotipagem de isolados de Mycobacterium bovis, provenientes de amostras de tecidos de bovinos positivos no teste cervical comparativo (TCC) para tuberculose em Mato Grosso do Sul, por meio da técnica de spoligotyping.
Tipo: Artigo de periódico Palavras-chave: Molecular identification; Poligotyping.; Mycobacterium Bovis.; Bovine tuberculosis; Molecular epidemiology..
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1015208
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Identification and phylogenetic inferences on stocks of sharks affected by the fishing industry off the Northern coast of Brazil Genet. Mol. Biol.
Rodrigues-Filho,Luis Fernando da Silva; Rocha,Tainá Carreira da; Rêgo,Péricles Sena do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
The ongoing decline in abundance and diversity of shark stocks, primarily due to uncontrolled fishery exploitation, is a worldwide problem. An additional problem for the development of conservation and management programmes is the identification of species diversity within a given area, given the morphological similarities among shark species, and the typical disembarkation of processed carcasses which are almost impossible to differentiate. The main aim of the present study was to identify those shark species being exploited off northern Brazil, by using the 12S-16S molecular marker. For this, DNA sequences were obtained from 122 specimens collected on the docks and the fish market in Bragança, in the Brazilian state of Pará. We identified at least 11...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sharks; Molecular identification; Phylogenetic; MtDNA; Conservation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200031
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Infección cruzada de especies de Colletotrichum en frutos tropicales en postcosecha. Colegio de Postgraduados
Aguilar Pérez, Luis Alfonso.
El género Colletotrichum es considerado uno de los principales hongos patógenos de plantas, este hongo se encuentra distribuido a escala mundial y afecta a diferentes cultivos tanto en pre y postcosecha. En el presente trabajo se aislaron e identificaron morfológicamente y molecularmente ocho aislamientos de Colletotrichum sp, de banano, papaya, carambolo, chile manzano, aguacate, mango ataulfo y mango manila, estos se inocularon sobre cada uno de los frutos para evaluar el fenómeno de infección cruzada. Se midió diariamente el diámetro de la lesión por siete días. Los resultados mostraron que algunos aislamientos de C. gloeosporioides son más agresivos cuando se inoculan sobre su hospedero original, sin embargo ocasionan daños en menor grado en hospederos...
Palavras-chave: Colletotrichum; Frutos tropicales; Identificación molecular; Infección cruzada; Tropical fruits; Molecular identification; Cross infection; Maestría; Fitopatología.
Ano: 2010 URL: http://hdl.handle.net/10521/306
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Molecular assessment of Gymnotus spp. (Gymnotiformes: Gymnotidae) fishing used as live baitfish in the Tietê River, Brazil Neotropical Ichthyology
Faria-Pereira,Lilian Paula; Hilsdorf,Alexandre Wagner Silva; Albert,James; Paiva,Maria José Tavares Ranzani; Galvão,Márcia Santos Nunes.
ABSTRACT The capture of live bait for sport fishing is an important activity for fishing communities. The main species used for this purpose are members of the genus Gymnotus, which comprises numerous species of cryptic nature that are difficult to identify based on external morphology. The aims of this work were to identify through partial sequences of the COI gene Gymnotus species fished in the Jacaré-Guaçu River, SP, and to develop a molecular diagnostic approach using PCR-RFLP to identify these species. Partial COI sequences were compared to those of other species deposited in GenBank. The sequences were assessed in the NEBCutter program to determine restriction sites in the sequence and the enzymes to be tested. Phenetic analysis performed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular identification; PCR-RFLP; Tuvira.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000500205
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Molecular prevalence and factors associated with Babesia vogeli infection in dogs in the Cerrado Mato-Grossense region of Brazil Ciência Rural
Castro,Vanessa Vieira; Ayres,Eveline da Cruz Boa Sorte; Canei,Darlan Henrique; Pereira,Mariana Elisa; Sousa,Valéria Régia Franco; Chitarra,Cristiane Silva; Dutra,Valéria; Nakazato,Luciano; Almeida,Arleana do Bom Parto Ferreira de.
ABSTRACT: Canine babesiosis is a common haemoparasitosis in Brazil. Caused by parasites of the genus Babesia, it is transmitted by ixodid ticks and affects domestic and wild canids. The objective of this study was to verify the prevalence of Babesia species (spp.) using molecular methods in dogs living in urban and rural areas of Cuiabá, Mato Grosso State, Brazil, and to identify the main factors associated with infection. A total of 407 samples from 407 dogs were evaluated using a polymerase chain reaction (PCR) technique, among which Babesia species (spp.) was amplified in 10 (2.5%). Although, no statistical association was found among the variables studied (p>0.05), greater positivity was observed in dogs<1 year of age, male sex, those with free...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Babesiosis; Molecular identification; Cuiaba.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200500
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Morphological, biochemical, and molecular characterization of Meloidogyne spp. populations from Brazilian soybean production regions Ciência Rural
Oliveira,Camilla Martins de; Souza Junior,Ismail Teodoro; Araújo Filho,Jerônimo Vieira de; Freitas,Marcos Augusto de; Rocha,Mara Rúbia da; Petrofeza,Silvana.
ABSTRACT: Soybean is a commodity of great economic importance worldwide, particularly in Brazil, world’s second largest producer. Nematodes, especially those of the Meloidogyne genus, severely limit productivity. Identification of nematode species is important for effective soybean management. Here, 26 populations of root-knot nematode (Meloidogyne spp.) from 15 municipalities in the states of Bahia, Mato Grosso, Goias, and Minas Gerais were characterized based on the morphology of the female perineal region, esterase profile, and identification based on amplification of specific regions of the population genome. Among the Meloidogyne spp. populations obtained, M. incognita and M. javanica, were identified. No mixed populations were present in the samples....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Root-knot nematode; Glycine max; Esterase; Molecular identification.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000500151
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