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| Registros recuperados: 26 | |
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| Clasen,Bárbara Estevão; Silveira,Andressa de Oliveira; Baldoni,Daiana Bortoluzzi; Montagner,Daiane Fiuza; Jacques,Rodrigo Josemar Seminoti; Antoniolli,Zaida Inês. |
| ABSTRACT: Mycorrhizae are mutualistic associations between fungi and plant roots. These symbiotic associations are abundant and occur in 75 to 80 % of plants. Ectomycorrhizal fungi are very important in ecosystems, because their mutualistic association with plants of different species helps nutrients and water absorption, as well as protection of the host plant against pathogens and abiotic stresses. Most ectomycorrhizal fungi belong to the Basidiomycota class, such as the following genera: Amanita, Hebeloma, Hysterangium, Laccaria, Lactarius, Rhizopogon, Russula, Scleroderma, Suillus, Tricholoma, among others. Morphological studies on ectomycorrhizae report important results in understanding the species biodiversity. However, the use of molecular biology... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PCR; Ectomycorrhizal fungi; Molecular identification; Morphotype. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000300246 |
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| Diniz,S.; Sandes,S.H.C.; Bomfim,M.R.Q.; Santos,P.C.; Cruz,F.D.; Moreira,T.F.; Carvalho,M.R.S.; Cisalpino,P.S.; Moreira,E.S. A.. |
| ABSTRACT Bovine digital dermatitis (BDD) is an infectious and contagious disease characterized by ulcerative and proliferative lesions affecting the skin on the bulbs of the heel or the interdigital cleft in dairy cattle, often associated with lameness. Evidences on the etiology of BDD indicate that it is multifactorial, involving environmental factors and multiple bacterial colonization. We isolated and identified microorganisms from BDD biopsy samples obtained from five Holstein Friesian and two Jersey cows by cultivation and molecular identification of bacterial isolates using 16S rRNA gene sequence analysis. We identified six bacterial species: Spirochetes as Treponema pedis and Leptospira broomi/L. fainei, L. licerasiae/L. wolffii; Corynebacterium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine digital dermatitis; Molecular identification; 16S rRNA gene analysis; Treponema; Leptospira. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000300559 |
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| PIE,MARCIO R.; STRÖHER,PATRÍCIA R.; AGOSTINIS,ANDRÉ O.; BELMONTE-LOPES,RICARDO; TADRA-SFEIR,MICHELLE Z.; OSTRENSKY,ANTONIO. |
| ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EDNA; Invasive species; Environmental DNA; Molecular identification. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301041 |
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| ALMEIDA,Silvana de F. Oliveira de; SILVA,Letícia R Carvalho; JUNIOR,Gilson Celso A. Chagas; OLIVEIRA,Guilherme; SILVA,Silvia Helena Marques da; VASCONCELOS,Santelmo; LOPES,Alessandra Santos. |
| ABSTRACT The purpose of this study was to identify the yeasts involved in spontaneous fermentation of cocoa from the Brazilian Amazon region. The fermentation process was carried out experimentally with cocoa seeds from two sites (Medicilândia and Tucumã), State of Pará, northern Brazil, during a six-day period. Totals of 44 yeasts were isolated from Medicilândia and 29 from Tucumã. Molecular identification was carried out by sequencing the D1/D2 region fragment of the rRNA 26S gene, expanded with universal primers for the NL1GC and LS2 eukaryotes. Pichia manshurica and Saccharomyces cerevisiae were identified in Medicilândia and five yeast species (Pichia fermentans, P. kudriavzevii, P. manshurica, S. cerevisiae and Zygosaccharomyces bailii) were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Accession; Molecular identification; Cocoa beans; Pichia Manshurica; Saccharomyces cerevisiae; Theobroma cacao. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672019000100064 |
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| RAMÍREZ-CASTRILLÓN,MAURICIO; USMAN,LUISA M.; SILVA-BEDOYA,LINA M.; OSORIO-CADAVID,ESTEBAN. |
| Abstract: The biotechnological potential of yeasts associated to different habitats in Colombia has been poorly studied, especially the yeasts associated with different plant structures. Fruit pulps are interesting substrates mainly for the growth of yeast species, that can positively affect the productivity and quality of some bioeconomic species. Therefore, the objective of this study was to identify the dominant yeast species associated with mango and rose apple fruit pulps in Cali, Colombia. A total of 90 isolates were obtained, which were grouped considering their colony morphology. The D1/D2 domain of the large ribosomal RNA gene (LSU rRNA gene) or internal transcribed spacer (ITS) 1, ribosomal gene 5.8S and ITS 2 (ITS) regions of one to several... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mangifera indica; Syzygium malaccense; Molecular identification; Yeasts; Colombia. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700626 |
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| Leão,Evelynne Urzêdo; Spadotti,David Marques de Almeida; Rocha,Kelly Cristina G.; Lima,Élison Fabricio B.; Tavella,Luciana; Turina,Massimo; Krause-Sakate,Renate. |
| ABSTRACT Identification of Thysanoptera is based mainly on external morphology examination that can be time-consuming and difficult for non taxonomic experts. In this work, we propose a rapid and efficient molecular method to identify Frankliniella schultzei, an important and widespread pest thrips vector of tospoviruses in South America countries. Species-specific primers designed in the mitochondrial cytochrome oxidase I gene (mtCOI) were optimized for detection by conventional PCR and real-time PCR. The primers were tested on immature and adult thrips collected from crops and weeds found in São Paulo State. All samples collected were identified as F. schultzei, indicating the high prevalence of this species as vector of tospoviruses in Brazilian fields. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular identification; Real-time PCR; Tospovirus vector. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100454 |
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| MAIA, N. da C.; SOUZA, P. N. da C.; GODINHO, B. T. V.; MOREIRA, S. I.; ABREU, L. M. de; JANK, L.; CARDOSO, P. G.. |
| This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi inhibited... |
| Tipo: Artigo de periódico |
Palavras-chave: Antagonism; Molecular identification; RDNA; Grasses; Plant pathogens. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1110371 |
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| Maia,Natália da Costa; Souza,Patrícia Nirlane da Costa; Godinho,Bárbara Temponi Vilarino; Moreira,Silvino Intra; Abreu,Lucas Magalhães de; Jank,Liana; Cardoso,Patrícia Gomes. |
| ABSTRACT This study aimed to isolate and identify endophytic fungi from the forage grass P. maximum and evaluate their ability to inhibit the growth of plant pathogenic fungi. One sample from P. purpureum grass was also included. Surface disinfected stem fragments were used for endophytic fungal isolation. One hundred and twenty-six endophytic fungi were isolated, of which 118 were from P. maximum and eight from P. purpureum. Morphological characteristics and internal transcribed spacer (ITS) and 18S (NS) sequence comparisons identified most isolated endophytic fungi as belonging to the phylum Ascomycota, with Sarocladium being the dominant genus. The isolates were subjected to in vitro antagonism tests against pathogenic fungi, and 31 endophytic fungi... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antagonism; Grasses; Molecular identification; Plant pathogens; RDNA. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100303 |
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| Rodrigues-Filho,Luis Fernando da Silva; Rocha,Tainá Carreira da; Rêgo,Péricles Sena do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo. |
| The ongoing decline in abundance and diversity of shark stocks, primarily due to uncontrolled fishery exploitation, is a worldwide problem. An additional problem for the development of conservation and management programmes is the identification of species diversity within a given area, given the morphological similarities among shark species, and the typical disembarkation of processed carcasses which are almost impossible to differentiate. The main aim of the present study was to identify those shark species being exploited off northern Brazil, by using the 12S-16S molecular marker. For this, DNA sequences were obtained from 122 specimens collected on the docks and the fish market in Bragança, in the Brazilian state of Pará. We identified at least 11... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sharks; Molecular identification; Phylogenetic; MtDNA; Conservation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200031 |
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| Aguilar Pérez, Luis Alfonso. |
| El género Colletotrichum es considerado uno de los principales hongos patógenos de plantas, este hongo se encuentra distribuido a escala mundial y afecta a diferentes cultivos tanto en pre y postcosecha. En el presente trabajo se aislaron e identificaron morfológicamente y molecularmente ocho aislamientos de Colletotrichum sp, de banano, papaya, carambolo, chile manzano, aguacate, mango ataulfo y mango manila, estos se inocularon sobre cada uno de los frutos para evaluar el fenómeno de infección cruzada. Se midió diariamente el diámetro de la lesión por siete días. Los resultados mostraron que algunos aislamientos de C. gloeosporioides son más agresivos cuando se inoculan sobre su hospedero original, sin embargo ocasionan daños en menor grado en hospederos... |
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Palavras-chave: Colletotrichum; Frutos tropicales; Identificación molecular; Infección cruzada; Tropical fruits; Molecular identification; Cross infection; Maestría; Fitopatología. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/306 |
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| Castro,Vanessa Vieira; Ayres,Eveline da Cruz Boa Sorte; Canei,Darlan Henrique; Pereira,Mariana Elisa; Sousa,Valéria Régia Franco; Chitarra,Cristiane Silva; Dutra,Valéria; Nakazato,Luciano; Almeida,Arleana do Bom Parto Ferreira de. |
| ABSTRACT: Canine babesiosis is a common haemoparasitosis in Brazil. Caused by parasites of the genus Babesia, it is transmitted by ixodid ticks and affects domestic and wild canids. The objective of this study was to verify the prevalence of Babesia species (spp.) using molecular methods in dogs living in urban and rural areas of Cuiabá, Mato Grosso State, Brazil, and to identify the main factors associated with infection. A total of 407 samples from 407 dogs were evaluated using a polymerase chain reaction (PCR) technique, among which Babesia species (spp.) was amplified in 10 (2.5%). Although, no statistical association was found among the variables studied (p>0.05), greater positivity was observed in dogs<1 year of age, male sex, those with free... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Babesiosis; Molecular identification; Cuiaba. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200500 |
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| Oliveira,Camilla Martins de; Souza Junior,Ismail Teodoro; Araújo Filho,Jerônimo Vieira de; Freitas,Marcos Augusto de; Rocha,Mara Rúbia da; Petrofeza,Silvana. |
| ABSTRACT: Soybean is a commodity of great economic importance worldwide, particularly in Brazil, world’s second largest producer. Nematodes, especially those of the Meloidogyne genus, severely limit productivity. Identification of nematode species is important for effective soybean management. Here, 26 populations of root-knot nematode (Meloidogyne spp.) from 15 municipalities in the states of Bahia, Mato Grosso, Goias, and Minas Gerais were characterized based on the morphology of the female perineal region, esterase profile, and identification based on amplification of specific regions of the population genome. Among the Meloidogyne spp. populations obtained, M. incognita and M. javanica, were identified. No mixed populations were present in the samples.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Root-knot nematode; Glycine max; Esterase; Molecular identification. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000500151 |
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| Registros recuperados: 26 | |
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