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| Teruel,M; Catalano,R; Salomón,L. |
| The aim of this work was to evaluate the effect of albendazole sulphoxide (ABZSO) administered to Balb C mice prior to mating on fertilization rate and preimplantational embryo development. Twenty four female mice 5-8 weeks of age were superovulated by intraperitoneal injection of 7.5 UI of equine chorionic gonadotropin (eCG, Novormon®, Laboratorios Syntex S.A., Argentina); 48 h later they received 10 IU of human chorionic gonadotropin (hCG, Profasi®, Laboratorios Serono, Méjico) and were paired with males of proven fertility. Females received 100 mg/kg or 200 mg/kg of ABZSO orally at the time of hCG administration, prior to mating. The control group received carboxymethylcellulose, vehicle used to prepare the drug suspension. Pregnant females were killed... |
| Tipo: Journal article |
Palavras-chave: Albendazole Sulphoxide; Reproductive toxicity; Embryo development; Mouse. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300024 |
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| Birgani,Golshan Arzani; Ahangarpour,Akram; Khorsandi,Layasadat; Moghaddam,Hadi Fathi. |
| ABSTRACT Diabetes is a metabolic disease caused by abnormal insulin secretion or action. In the present study, the effects of betulinic acid (BA, a triterpene) are evaluated on glucose, α-amylase and plasma insulin levels, insulin resistance and the histopathology of pancreatic islets in streptozotocin-nicotinamide (STZ-NA) diabetic mice. Seventy adult male NMRI mice were randomly divided into seven groups: control, sham, diabetic, diabetic treated with BA (10, 20 and 40 mg/kg) and diabetic treated with metformin (200 mg/kg). Diabetes was induced in mice by intraperitoneal injection of streptozotocin 50 mg/kg after a dose of nicotinamide 120 mg/kg. Two weeks after treatment with BA, blood samples were collected for measuring glucose, α-amylase and insulin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Betulinic acid; Diabetes; Mouse; Streptozotocin-nicotinamide.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200621 |
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| Cunha,R.M.C.; Lavalle,G.E.; Reis,D.C.; Horta,R.S.; Teixeira,S.V.; Ramirez,J.A.; Araújo,R.B.. |
| ABSTRACT Electrochemotherapy is a local anticancer treatment in which non-permeant chemotherapeutic drugs are associated with electric pulses of well-established parameters. The electric pulses cause pores to open on the plasma membrane and facilitate drug transport, enhancing cytotoxicity and reducing side effects. Assessment of electrochemotherapy effects on Ehrlich solid tumor development in this work aims to evaluate in vivo usage of the electroporator device developed by the Department of Electrical Engineering of Engineering School of UFMG. Therefore, 40 Swiss mice were inoculated with Ehrlich tumor cells, and developed the tumor in solid form. After 21 days, mice were subjected to specific treatment protocols (control, bleomycin, electric pulses and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mouse; Oncology; Electrochemotherapy; Experimental tumor. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000601581 |
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| Bustos-Obregón,Eduardo; Olivares,Christopher. |
| It has been reported that boron causes changes in various systems, including the male reproductive system. Residents in some towns in northern Chile were consuming a few years ago in the drinking water 20 times more than the amount established as permissible limit by WHO. This study evaluates the effects in an animal model of high intake of boron on the testis. Boron was administered in the drinking water. Twenty male mice (Mus domesticus), sexually mature, were used, divided into 2 groups: the experimental group was given Boron at a dose of 12 mg/L, and the control group 0.6 mg/L, for 42 days. Sections of testis were obtained for: HE staining (Morphometry and Histopathology), Immunohistochemistry (Cox-2), Mallory and Picrosirious stain (evaluation of... |
| Tipo: Journal article |
Palavras-chave: Boron; Mouse; Testicular structure; Collagen I/III; Infertility. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300057 |
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| CHENG,SHU-QUN; HE,JUN-LIN; DONG,YAN-LING; LIU,XUE-QING; DING,YU-BIN; GAO,RU-FEI; TAN,YI; YE,QIAN; TIAN,ZHEN-LING; WANG,YING-XIONG. |
| Calreticulin (CRT), a Ca2+-binding storage protein and chaperone in the endoplasmic reticulum, modulates cell adhesiveness and integrin-dependent Ca2+ signaling. However, the role of CRT during implantation remains poorly understood. In the present study, we characterized the expression of CRT mRNA and the protein in mouse endometria from pregnancy DI to D7. Real-Time PCR and in situ hybridization results showed that the levels of CRT mRNA in the endometria of pregnant mice were significantly higher than those of non-pregnant mice (P<0.05), and increased gradually from pregnancy DI to D4, reaching the máximum level on D4, followed by a plateau from D4 to D7. Using immunofluorescence histochemistry and western blot, changes of CRT expression in the... |
| Tipo: Journal article |
Palavras-chave: Calreticulin; Endometrium; Embryo implantation; Mouse. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400012 |
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| Makala, Levi H. C; Zayatiin, Batsukh; Kamada, Takenori; Seng, Seyha; Ribas, Lazaro; Mishima, Masayuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Nagasawa, Hideyuki. |
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Palavras-chave: Spleen; Dendritic cell; Mouse; Toxoplasma lysate antigen (TLA). |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/625 |
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| LÓPEZ-SOLÍS,REMIGIO; PUENTE,MARGARITA; DURÁN,VIVIANA; MORALES-BOZO,IRENE; KEMMERLING,ULRIKE; PARDO,RODRIGO; WENK,CAROLINA. |
| Mouse salivary secretion provoked by single or multiple oral administrations of the cholinergic agent pilocarpine was characterized. An accessory ad hoc device, manipulated by a single operator, was used to collect saliva from various mice simultaneously. A single challenge by pilocarpine in the range 40-400 µg provoked a dose-dependent secretory response. The secretory response lasted for about 40 minutes and was constituted by four clearly defined stages, namely: lag (5-10 min), maximal flow (10 min), slowering (15-20 min) and postsecretory rest. In this response, usual parameters were: maximal flow rate, 30-40 µl/min; total volume of saliva, 250-350 µl/mouse and total salivary protein, about 700 µg/mouse. Temporal desensitization of the secretory... |
| Tipo: Journal article |
Palavras-chave: Mouse; Saliva; Secretion; Pilocarpine; Polypeptides. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000023 |
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| YAMADA, Manabu; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; MATSUI, Takane; 古林, 与志安; 古岡, 秀文. |
| Autumn crocus poisoning of cattle is characterized by severe diarrhea caused by alkaloid colchicine. Previously, we examined pathologically this poisoning in cattle and reported that enterotoxic lesions were closely associated with apoptosis. To examine enterotoxicity of autumn crocus more precisely, a reproductive study was performed using guinea pigs and mice, and pathological findings associated with autumn crocus poisoning were compared with those of colchicine. Each group of guinea pigs given the bulb of autumn crocus or colchicine exhibited severe diarrhea. Histopathological findings in intoxicated guinea pigs were entirely consistent with those in the autumn crocus-poisoned cattle. In contrast, each group of mice administered with the bulb or... |
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Palavras-chave: Apoptosis; Autumn crocus (Colchicum autumnale L.); Colchicine; Guinea pig; Mouse. |
| Ano: 2000 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4170 |
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| Grodzki,A.C.G.; Dávila Pástor,M.V.; Sousa,J.F.; Oliver,C.; Jamur,M.C.. |
| Mast cell progenitors arise in bone marrow and then migrate to peripheral tissues where they mature. It is presumed that integrin receptors are involved in their migration and homing. In the present study, the expression of various integrin subunits was investigated in three systems of adherent and nonadherent mast cells. Mesentery mast cells, freshly isolated bone marrow-derived mast cells (BMMC) and RBL-2H3 cells grown attached to tissue culture flasks are all adherent mast cells and peritoneal mast cells, and cultured BMMC and RBL-2H3 cells grown in suspension represent nonadherent mast cell populations. Pure populations of mast cells were immunomagnetically isolated from bone marrow, mesentery and peritoneal lavage using the mast cell-specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mast cells; Integrin; Mouse. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800017 |
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| Picazo,María G; García-Olmo,Dolores C. |
| Background Genotyping of mice is a common procedure in animal facilities. The aim of this study was to compare the quantity and quality of DNA extracted from samples obtained from young mice (YM; 10 d old) and adult mice (AM; 12 weeks old). We collected samples from the tail and ear of YM and AM. We also sampled blood, check cells (via buccal swabs), hair and fecal pellets of AM, and biopsied distal phalanx of YM. We isolated DNA using commercial kits and determined concentrations and purity by spectrophotometry. The integrity of DNA was evaluated by agarose-gel electrophoresis and polymerase chain reaction (PCR). Results DNA in all samples was amplified successfully but the intensities of bands after electrophoresis was heterogeneous. In general, tissues... |
| Tipo: Journal article |
Palavras-chave: DNA extraction; Genotyping; Mouse. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200004 |
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| Bustos-Obregón,Eduardo; Esponda,Pedro; Sarabia,Luis. |
| The spermatogenesis is regulated by the hypotalamus-hypophysis gonadal axis, and the androgens play a fundamental role in their last stages. The administration of the antiandrogen flutamide interferes with the function of androgen dependent organs (Prostate (P) and Seminal Vesicle (VS)). In this study flutamide (10mg/Kg b.w.) was injected to 10 mice and vehicle to the controls (n=6). The mice were sacrificed at 24 (n=5) and 72 (n=5) hours. In testicular sections the tubular diameter (TD) and height of seminiferous epithelium (EH) were measured. P and VS were macerated, and concentrations of fructose (VS) and zinc (P) were determined. No significant difference exists in the DT among the groups. However, the 72 hrs group showed EH higher than the control (p... |
| Tipo: Journal article |
Palavras-chave: Flutamide; Mouse; Spermatogenesis; Seminal vesicles; Prostate. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300008 |
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| Freitas,E. S.; Leite,E. D.; Silva,A. E.; Ocarino,N. M.; Ferreira,E.; Gomes,M. G.; Cassali,G. D.; Serakides,R. |
| The main purpose of this study was to investigate the effect of thyroxine and PTU in ascitic Ehrlich tumor cells. Tumor was implanted in 30 female mice distributed in three groups: treated with PTU, treated with thyroxine and control. Each group received an intraperitoneal injection of neoplastic cells, pre-incubated with sterile solutions of PTU, thyroxine and distilled water, respectively. On the fifth and seventh days after inoculation, animals received an intraperitoneal injection of the respective solutions. On the tenth day after inoculation, animals were sacrificed. Volume of ascitic liquid, number of neoplastic cells/ml and percentage of viable cells were determined. Ascitic liquid smears were carried out for tumor cytological evaluation. There was... |
| Tipo: Journal article |
Palavras-chave: Cytology; Ehrlich tumor; Mouse; NORs; Propylthiouracil; Thyroxine. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500024 |
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| Bustos-Obregón,Eduardo; Esveile,Christian; Contreras,Julio; Maurer,Inge; Sarabia,Luis. |
| Reduction of O2 delivery to tissues damage them, including the seminiferous epithelium. Recently, population working in high altitude has increased, so that the study of hypobaric hypoxia on spermatogenesis becomes of interest. In this study we used two groups of male, sexually mature mice Control (C) (540 meters above sea level (masl)) and chronic simulated hypobaric hypoxia (CSHH) (4,600 masl) exposed during 8, 16, 24 or 33 days. Hematocrit; reticulocytosis; testicular, epididymal and seminal vesicle weight; seminiferous epithelium height, tubular diameter, sperm count and morphology and testicular parenchyme and spermatozoa membranes lipoperoxidation were measured. Weight of testis, epididymis and seminal vesicle were reduced but they recuperate at 33... |
| Tipo: Journal article |
Palavras-chave: Hypoxia; Lipoperoxidation; Mouse; Testis. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400030 |
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| Verçosa Júnior,D.; Ferraz,V.P.; Duarte,E.R.; Oliveira,N.J. F.; Soto-Blanco,B.; Cassali,G.D.; Melo,M.M.. |
| The aim of this study was to investigate the effect of the mushroom Agaricus blazeii Murril (ABM) extracts on the hematological profile of Swiss mice bearing an Ehrlich solid tumor. Three fractions (total extract, polysaccharides, and supernatant) of ABM extracts obtained by four methods (ultrasonic or water bath, at pH 4 or pH 7) were administered to mice over 21 days. Polysaccharide solutions were analyzed by gas and liquid chromatography that showed both mannose and glucose concentrations. The method of extraction influenced the degree of glucose polymerization and the mannose/glucose relationship. The treatment with ABM supernatant at pH 7 and water bath was associated with reduced concentrations of leukocytes and lymphocytes and altered the percentage... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mouse; Ehrlich solid tumor; Polysaccharides; Hematological profile. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300679 |
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| Vargas,Álvaro; Bustos-Obregón,Eduardo; Hartley,Ricardo. |
| Hypobaric hypoxia is of interest due to an increase of human populations working at high altitude. Testicular damage is related to the physiological response (neoangiogenesis) to increased intrascrotal blood flow as temperature rises. Hypoxia is a stress factor with overproduction of reactive oxygen species (ROS). The effect of hypoxia in mice reproductive parameters is analyzed. Animals were exposed to simulated hypoxia of 4,200 meters above sea level (m.a.s.l.) in a chamber for 33.2 days, both to continuous (HH) or intermittent hypoxia (HI) with an intermittency period of 4 days hypoxia /4 days normoxia (500 m.a.s.l.). The anti-inflammatory drug Ibuprofen was administered to a group of mice to control vasodilation and increased blood flow. Melatonin was... |
| Tipo: Journal article |
Palavras-chave: Epididymal sperm; Mouse; Ibuprofen; Melatonin. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200008 |
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