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Registros recuperados: 83
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A test of the utility of DNA barcoding in the radiation of the freshwater stingray genus Potamotrygon (Potamotrygonidae, Myliobatiformes) Genet. Mol. Biol.
Toffoli,Daniel; Hrbek,Tomas; Araújo,Maria Lúcia Góes de; Almeida,Maurício Pinto de; Charvet-Almeida,Patricia; Farias,Izeni Pires.
DNA barcoding is a recently proposed global standard in taxonomy based on DNA sequences. The two main goals of DNA barcoding methodology are assignment of specimens to a species and discovery of new species. There are two main underlying assumptions: i) reciprocal monophyly of species, and ii) intraspecific divergence is always less than interspecific divergence. Here we present a phylogenetic analysis of the family Potamotrygonidae based on mitochondrial cytochrome c oxidase I gene, sampling 10 out of the 18 to 20 valid species including two non-described species. Potamotrygonidae systematics is still not fully resolved with several still-to-be-described species while some other species are difficult to delimit due to overlap in morphological characters...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Potamotrygon; Barcoding; Radiation; Phylogenetics; COI; MtDNA.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200028
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Additional results and discussion to the paper: Asymmetric viability of reciprocal-cross hybrids between crested and marbled newts (Triturus cristatus and T. marmoratus) Naturalis
Arntzen, J.W.; Jehle, R.; Bardakci, F.; Burke, T.; Wallis, G.P..
Hybridization between divergent lineages often results in reduced hybrid viability. Here we report findings from a series of independent molecular analyses over several seasons on four life stages of F1 hybrids between the newts Triturus cristatus and T. marmoratus. These two species form a bimodal hybrid zone of broad overlap in France, with F1 hybrids making up about 4% of the adult population. We demonstrate strong asymmetry in the direction of the cross, with one class (cristatus-mothered) making up about 90% of F1 hybrids. By analyzing embryos and hatchlings, we show that this asymmetry is not due to prezygotic effects, as both classes of hybrid embryos are present at similar frequencies, implicating differential selection on the two hybrid classes...
Tipo: Article / Letter to the editor Palavras-chave: Allozyme; Bateson–Dobzhansky–Muller incompatibility; Cytonuclear incompatibility; Hybridization; Microsatellite; MtDNA; Postzygotic isolation asymmetry; Triturus; 42.82.
Ano: 2008 URL: http://www.repository.naturalis.nl/record/289893
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Análise filogeográfica de populações selvagens e em cativeiro do Bijupirá (Rachycentron canadum)na costa brasileira. Repositório Alice
NEPOMUCENO, A. R.; FARIA, D. A. de; CAVALCANTI, L. C. G.; BIAZIO, G. R. de; SILVA, N. M. A. da; SANCHES, E. G.; CARNEIRO, P. C. F.; MARIA, A. N.; BRAVO, I. A. F.; FOGACA, F. H. S.; MCMANUS, C.; CAETANO, A. R.; PAIVA, S. R..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Cobia; ATP6; MtDNA.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1000941
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Characterization of six rat strains (Rattus norvegicus) by mitochondrial DNA restriction fragment length polymorphism BJMBR
Hilsdorf,A.W.; Krieger,J.E..
Restriction fragment length polymorphism (RFLP) was used to examine the extent of mtDNA polymorphism among six strains of rats (Rattus norvegicus) - Wistar, Wistar Munich, Brown Norway, Wistar Kyoto, SHR and SHR-SP. A survey of 26 restriction enzymes has revealed a low level of genetic divergence among strains. The sites of cleavage by EcoRI, NcoI and XmnI were shown to be polymorphic. The use of these three enzymes allows the 6 strains to be classified into 4 haplotypes and identifies specific markers for each one. The percentage of sequence divergence among all pairs of haplotypes ranged from 0.035 to 0.33%, which is the result of a severe population constriction undergone by the strains. These haplotypes are easily demonstrable and therefore RFLP...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Restriction fragment length polymorphism (RFLP); Rattus norvegicus; MtDNA; Laboratory rats; Genetic monitoring.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000300003
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Cytogenetic and molecular markers reveal the complexity of the genus Piabina Reinhardt, 1867 (Characiformes: Characidae) Neotropical Ichthyology
Pazian,Marlon Felix; Pereira,Luiz Henrique Garcia; Shimabukuru-Dias,Cristiane Kioko; Oliveira,Claudio; Foresti,Fausto.
Cytogenetic and molecular analyses were carried out in fish representative of the genus Piabina. This study specifically involved the species P. argentea and P. anhembi collected from areas of the Paranapanema and Tietê River basins, Brazil. Our findings suggest that fish classified as Piabina argentea in the Paranapanema and Tietê Rivers may represent more than one species. The samples analyzed differed by cytogenetic particularities and molecular analyses using partial sequences of the genes COI and CytB as genetic markers revealed three distinct groups of P. argentea with genetic distances sufficient to support the conclusion that the three samples analyzed are three distinct taxonomic units.
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA Barcoding; Freshwater fishes; Fish; MtDNA; 18S rDNA; 5S rDNA.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000200008
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Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini) Neotropical Entomology
Moretto,Geraldo; Arias,Maria C..
The endemic range of the stingless bee Melipona quadrifasciata Lepeletier extends from the southern most state in Brazil, Rio Grande do Sul, up to the state of Paraíba, where it originally inhabited the Atlantic Rain Forest. Two subspecies have been morphologically recognized based on the tergal band pattern (yellow stripes across the abdomen), M. q. quadrifasciata and M. q. anthidioides. The objective of the present study was to characterize restriction site variation in the mitochondrial DNA of each subspecies. Four colonies of each subspecies were sampled. One individual per colony was used for DNA extraction and further analysis through amplification of nine fragments of the mitochondrial genome and digestion with 13 restriction enzymes (PCR+RFLP)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Restriction enzyme; Polymorphism; PCR+RLFP; MtDNA.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300004
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Development and characterization of two new cell lines from common carp, Cyprinus carpio (Linn) Biol. Res.
Lakra,Wazir S; Goswami,M; Rajaswaminathan,T; Rathore,Gaurav.
Two new cell lines (CCF and CCH) were established from fin and heart tissues of common carp, Cyprinus carpio. The cells were optimally maintained in Leibovitz-15 medium supplemented with 10% fetal bovine serum (FBS) and 10 ng/ml of basic fibroblastic growth factor (bFGF). The effects of temperature, concentration of FBS and bFGF on the growth of CCF and CCH cells were examined. The temperature ranged from 24 to 32 °C for good growth of the cells. The growth rate of cells was higher in medium containing 10% FBS and the addition of bFGF to the medium significantly increased the growth rate. The CCF cells were found to be epithelial, while the CCH cells were fibroblastic in nature. The cytogenetic analysis of the cell lines revealed a diploid number of 100...
Tipo: Journal article Palavras-chave: Cyprinus carpio; Cell line; MtDNA.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400002
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Differentiation of Melipona quadrifasciata L. (Hymenoptera, Apidae, Meliponini) subspecies using cytochrome b PCR-RFLP patterns Genet. Mol. Biol.
Souza,Rogério O.; Moretto,Geraldo; Arias,Maria C.; Del Lama,Marco A..
Melipona quadrifasciata quadrifasciata and M. quadrifasciata anthidioides are subspecies of M. quadrifasciata, a stingless bee species common in coastal Brazil. These subspecies are discriminated by the yellow stripe pattern of the abdominal tergites. We found Vsp I restriction patterns in the cytochrome b region closely associated to each subspecies in 155 M. quadrifasciata colonies of different geographical origin. This mitochondrial DNA molecular marker facilitates diagnosis of M. quadrifasciata subspecies matrilines and can be used to establish their natural distribution and identify hybrid colonies.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Subspecies differentiation; Melipona quadrifasciata; Cytochrome b; MtDNA; PCR-RFLP patterns.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300009
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Diversidad haplotípica en el manatí Trichechus manatus en Cuba: Resultados preliminares OceanDocs
Hernández Martínez, D.; Álvarez-Alemán, A.; Bonde, R. K.; Powell, J.A.; García-Machado, E..
El objetivo del presente trabajo fue obtener información preliminar acerca de la composición de haplotipos del ADN mitocóndrico (ADNmt) del manatí (Trichechus manatus) que habita en el archipiélago cubano. Se analizó un total de 13 individuos, 12 de Cuba y uno procedente de la Florida, EU. Las secuencias de un fragmento de 410 pb de la región de control del ADNmt (D-loop) permitieron identificar dos haplotipos. El haplotipo A1, único encontrado en la Florida (incluida la muestra aquí analizada), pero también presente en México, Puerto Rico y República Dominicana. Este haplotipo fue mayoritario (11 de los 12 individuos muestreados en Cuba). El segundo haplotipo fue el A3, referido anteriormente como endémico de Belice, se encontró en un individuo...
Tipo: Journal Contribution Palavras-chave: Genetic diversity; MtDNA.
Ano: 2013 URL: http://hdl.handle.net/1834/5252
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Diversidade genética de Apis mellifera iberiensis (Hymenoptera: Apidae) na região norte de Portugal IPB - Escola Superior Agrária
Souza, Larissa; Pinto, M. Alice; Moura, Inês; Baptista, Paula; Carvalho, Carlos Alfredo.
A abelha melífera, Apis mellifera L., distribui-se naturalmente na África, Médio Oriente e Europa. A adaptação à diversidade de condições ecológicas climáticas propiciou a evolução de mais de 24 subespécies. Estudos moleculares e morfológicos possibilitou agrupá-las em 4 linhagens evolutivas (A, M, C, O). As linhagens A e O incluem as subespécies que ocorrem em África e no Médio Oriente, respectivamente, as linhagens C e M incluem as subespécies europeias. Na Península Ibérica ocorre as linhagens A e M. Estas abelhas são atualmente consideradas a ser uma subespécie particular, Apis mellifera iberiensis , originada após uma hibridação natural entre linhagens A e M. Neste estudo pretendeu-se analisar a diversidade genética da abelha Apis mellifera iberiensis...
Tipo: ConferenceObject Palavras-chave: Apis mellifera; Diversidade genética; MtDNA; Teste DraI.
Ano: 2010 URL: http://hdl.handle.net/10198/3765
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D-loop mitochondrial genetic analysis in Abeerden angus old type from Argentina JBAG
Villegas Castagnasso,E.E; Rogberg-Muñoz,A; Prando  ,A.J; Baldo,A; Giovambattista,G.
The massive use of reproductive breeding technologies (mainly Artificial Insemination and Embryo Transfer) resulted in an important decrease in the diversity in many breeds, and some genetic lines have been lost. Within the Angus breed, the “New Type” gain importance since 1970, leaving the “Old Type” to a reduced number of herds in the whole world. The objective of this work was to determine the genetic diversity in an “Old Type” herd in Argentina. DNA samples were analyzed for sequence variation in the hypervariable region of the mitochondrial DNA (D-loop). Sequence comparison and phylogenetic analyses revealed that haplotypes fell into European haplogroup (T3) in general, and in particular had...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MtDNA; Bos Taurus; Angus; Matrilineages origin; Genetic diversity.
Ano: 2015 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332015000300003
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Evidence of African honey bees mitotypes in the southern United States prior to Africanization as revealed by mtDNA sequence data IPB - Escola Superior Agrária
Pinto, M. Alice; Sheppard, Walter S.; Johnston, J. Spencer; Rubink, William L.; Coulson, Robert N.; Patton, John C..
The standard protocol for assessing honey bees with a mitotype derived from an African origin is the amplification of a segment of cytochrome b and subsequent digestion of the amplified fragment with the restriction enzyme Bgl II. A previous survey of 451pre-Africanization honey bees from the southern U. S. revealed three bees with restriction pattern consistent with an African mitotype. To confirm these bees truly represented mitotypes of African origin we developed a new primer pair for amplification of cytochrome b, which utilizes internal sequencing primers to allow high quality direct sequence products. Using this system we amplified a mtDNA fragment of ~1,200 base-pairs (bp), taht included most of cytochrome b, serine (UCA) tRNA, and a small portion...
Tipo: ConferenceObject Palavras-chave: Africanized honey bees; Sequence data; Cytochrome b; MtDNA.
Ano: 2004 URL: http://hdl.handle.net/10198/3807
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Evidence of geographical structuring in the Malaysian Snakehead, Channa striata based on partial segment of the CO1 gene Genet. Mol. Biol.
Siti-Balkhis,Abu Bakar; Jamsari,Amirul Firdaus Jamaluddin; Hwai,Tan Shau; Yasin,Zulfigar; Siti-Azizah,Mohd Nor.
Channa striata, locally known as "haruan", is economically important in fisheries and aquaculture industries in several Asian countries. DNA sequencing, based on a partial segment of the Cytochrome oxidase c subunit 1 (CO1) gene, was used to determine genetic variation in C. striata samples from four different populations on the west coast of Peninsular Malaysia. The highest nucleotide and haplotype diversities were observed in the Linggi population (π = 0.0067, h = 0.835), and the lowest in the Timah Tasoh population (π = 0.0008, h = 0.286). Apart from Kajang-Linggi, which was insignificant, F ST values were significant (p < 0.05) in all pairwise-population comparisons. Consequently, it is inferred that genetic structuring C. striata populations in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Channa striata; Snakehead; MtDNA; CO1; Isolation by distance.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300024
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Genetic and morphometric differences between yellowtail snapper (Ocyurus chrysurus, Lutjanidae) populations of the tropical West Atlantic Genet. Mol. Biol.
Vasconcellos,Anderson V.; Vianna,Paulo; Paiva,Paulo C.; Schama,Renata; Solé-Cava,Antonio.
Populations of Ocyurus chrysurus were compared genetically and morphometrically along the West Atlantic coast to test the null hypothesis of population homogeneity in the area. Brazilian populations were found to be differentiated in shape (canonical variates analysis; F[48,515] = 10.84, p < 0.0001). Analyses of mitochondrial DNA sequences (663 bp of the control region) did not show any differences between Brazilian populations but could detect differences between Brazilian and Caribbean (Belize) populations. The samples from Pernambuco differed significantly from the other Brazilian populations in allozyme frequencies (11 loci; F ST = 0.167; p < 0.05), but this may have resulted from the small number of samples analysed for that population. Sequence...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MtDNA; Allozymes; Morphometry; Fisheries; Phylogeography.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200026
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Genetic characterisation of populations of the critically endangered Goliath grouper ( Epinephelus itajara, Serranidae) from the Northern Brazilian coast through analyses of mtDNA Genet. Mol. Biol.
Silva-Oliveira,Gláucia C.; Rêgo,Péricles S. do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo.
The Goliath grouper ( Epinephelus itajara ) is one of the most endangered species of fish of the subfamily Epinephelinae. Slow to develop and mature, and dependent on mangrove habitats for breeding, the species also suffers intense harvesting, which has reduced drastically in numbers in many areas. To contribute to the understanding of the characteristics of E. itajara populations, we conducted a molecular genetics study of the species, focusing on populations from the Northern Brazilian coast. The mtDNA control region (D-loop) of 116 individuals from five localities (Bragança, Ajuruteua, Parnaíba, Fortaleza and Natal) was analysed, and a sequence of 499 base pairs identified. Analyses of the sequences indicated that genetic variability was generally...
Palavras-chave: Goliath grouper; Epinephelus itajara; Control region; MtDNA; Population genetics.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500029
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Genetic characterization of native and introduced populations of the neotropical cichlid genus Cichla in Brazil Genet. Mol. Biol.
Carvalho,Daniel Cardoso de; Oliveira,Denise Aparecida Andrade de; Santos,José Enemir dos; Teske,Peter; Beheregaray,Luciano B.; Schneider,Horacio; Sampaio,Iracilda.
A molecular phylogenetic analysis based on mitochondrial 16S ribosomal DNA and Control Region sequences from native and introduced populations was undertaken, in order to characterize the introduction of Cichla (peacock bass or tucunaré) species in Brazil. Mitochondrial DNA haplotypes found in introduced fish from Minas Gerais state (southeastern Brazil) clustered only with those from native species of the Tocantins River (Cichla piquiti and C. kelberi), thereby suggesting a single or, at most, few translocation acts in this area, even though with fish from the same source-population. Our study contributes to an understanding of the introduction of Cichla in regions of Brazil outside the Amazon basin, and adds phylogenetic data to the recently describe...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peacock bass or tucunaré; MtDNA; Invasive populations; Amazon basin; Minas Gerais.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300025
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Genetic distance between broodstocks of the marine shrimp Litopenaeus vannamei (Decapoda, Penaeidae) by mtDNA analyses Genet. Mol. Biol.
Francisco,Ana Karina de; Galetti Junior,Pedro Manoel.
In the constantly growing Brazilian shrimp industry the evaluation of genetic relationships between broodstocks is an useful tool for shrimp culture management programs. We established the genetic relationships between five broodstocks of the white marine shrimp Litopenaeus vannamei (Penaeidae) based on the sequencing of the mtDNA 16S rRNA and cytochrome oxidase I (COI) regions. Although no divergence was found between the broodstocks for the highly conservative 16S rRNA gene, we did find an 8.2% distance between L. vannamei and the Farfantepenaeus subtilis. Analyses of the COI region showed genetic distances of only 0.2 to 1% between the broodstocks, which contrasted with the 10.9% mean distance found between L. vannamei and F. subtilis. The small genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shrimp; Litopenaeus vannamei; Genetic variation; MtDNA.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200014
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Genetic diversity and population structure of different varieties of Morada Nova hair sheep from Brazil. Repositório Alice
FERREIRA, J. S. B.; PAIVA, S. R.; SILVA, E. C.; MCMANUS, C. M.; CAETANO, A. R.; FAÇANHA, D. A. E.; SOUSA, M. A. N..
bitstream/item/116096/1/gmr2908.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: MtDNA; Microsatellites; Genetic management; Ovis Aries; Animal genetic resources.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1006278
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Genetic Diversity of Mitochondrial DNA of Chinese Black-bone Chicken Rev. Bras. Ciênc. Avic.
Zhang,T; Du,W; Lu,H; Wang,L.
ABSTRACT The black-bone chicken has special economic value in Chinese poultry breeds, which also are valued for the medicinal properties of their meat in traditional Chinese medicine. In order to protect the genetic resources of native black-bone chicken breeds, we analyzed the genetic diversity and matrilineal components of 64 mtDNA D-loop partial sequences from three native black-bone chicken breeds, together with reported 596 black-bone chicken mtDNA sequences from China, Japan, and Korea. A total of 108 haplotypes were observed from 73 variable sites. These domestic chicken mtDNA sequences could be assigned into seven clades (A-G). The results indicated that 71.97% of the black-bone haplotypes were related to the reference sequence that may originate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chinese Black-bone Chicken; MtDNA; D-loop region; Haplotype; Maternal origin.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300565
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Genetic population structure of the Swordfish (Xiphias gladius) in the southwest Indian Ocean: Sex-biased differentiation, congruency between markers and its incidence in a way of stock assessment ArchiMer
Muths, Delphine; Grewe, R.; Jean, Claire; Bourjea, Jerome.
Genetic variation was surveyed at 11 microsatellite loci and at 517 bp of the mitochondrial control region to investigate the presence of genetic stock structure in swordfish (Xiphias gladius) in four proximal localities of the southwest Indian Ocean. One aim of this study was to serve as a preliminary examination for congruency of structure detected by these two genetic markers, prior to conducting a more comprehensive basin-wide survey of the Indian Ocean and nearby surrounding areas. Analyses of multilocus microsatellite genotypes and mitochondrial control region sequences both revealed a great homogeneity between samples. Genetic diversity detected at the regional scale was not significantly higher than detected at the local scale. Results suggest that...
Tipo: Text Palavras-chave: Indian Ocean; Population genetic; MtDNA; Microsatellites; Swordfish.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6455.pdf
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