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Registros recuperados: 29
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Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes BJMBR
Machado,C.G.; Calado,R.T.; Garcia,A.B.; Falcão,R.P..
The multidrug resistance P-glycoprotein is a transmembrane efflux pump expressed by lymphocytes and is involved in their cytolytic activity. In the present study, we investigated the age-related changes of P-glycoprotein function in normal peripheral blood lymphocytes. Blood samples from 90 normal volunteers (age range, 0 to 86 years) were analyzed. P-glycoprotein function was assessed by the flow cytometric rhodamine 123 assay. P-glycoprotein function was highest in cord blood and progressively declined with age in peripheral blood T CD4+ and CD8+ cells. In contrast, P-glycoprotein function did not vary with age in CD19+ B or CD16+CD56+ natural killer cells. These data suggest that the decline in P-glycoprotein function in T CD4+ and CD8+ lymphocytes as a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: P-glycoprotein; Multidrug resistance; Lymphocytes; Rhodamine 123; Flow cytometry.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200006
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Analysis of drug resistance mutations in pulmonary Mycobacterium tuberculosis isolates in the Southern coastal region of Andhra Pradesh, India BJID
Prasad,Polu Giri; Jasmine,Mohammad Shaik; kanta,Kota Neela Mani; Deepthi,Karumanchi; Allam,Uday Sankar.
ABSTRACT Purpose and objectives Detection of drug resistance plays a crucial role in tuberculosis (TB) treatment and prevention of Mycobacterium tuberculosis (MTB) transmission. The aim of this study was to determine the levels and patterns of resistance of MTB isolates to two key anti-TB drugs (rifampicin, RIF and isoniazid, INH) and the type of mutations in drug resistance genes (rpoB, katG and inhA) of the isolates at the southern coastal region of Andhra Pradesh, India, using commercially available GenoType MTBDRplus assay under the Revised National TB Control Program. Methods GenoType MTBDRplus assay was performed on 2859 sputum smear-positive samples and the mutations in the genes responsible for resistance (rpoB, katG and inhA) were analyzed....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multidrug resistance; Mycobacterium tuberculosis; MTBDRplus assay; Mutations; Molecular detection.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000500281
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Bacteremia and meningitis caused by OXA-23-producing Acinetobacter baumannii – molecular characterization and susceptibility testing for alternative antibiotics BJM
Schuertz,Kamile Francine; Tuon,Felipe Francisco; Palmeiro,Jussara Kasuko; Conte,Danieli; Telles,João Paulo Marochi; Trevisoli,Lucas Eduardo; Dalla-Costa,Libera Maria.
Abstract Background: Carbapenem-resistant Acinetobacter baumannii infection is a concern in developing countries due to high incidence, few therapeutic options, and increasing costs. Objective: Characterize and analyze the antibiotic susceptibility patterns of carbapenem-resistant A. baumannii isolates and evaluate clinical data of meningitis and bacteremia caused by this microorganism. Methods: Twenty-six A. baumannii isolates from 23 patients were identified by MALDI-TOF and automated methods and genotyped using pulsed field genotyping electrophoresis. Clinical data and outcomes were evaluated. Susceptibility of isolates to colistin, tigecycline, meropenem, imipenem, and doxycycline was determined. Results: Mortality due to A. baumannii infections...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acinetobacter baumannii; Oxacillinases; Carbapenem; Multidrug resistance; Doxycycline.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500199
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Bloodstream infections caused by ESBL-producing E. Coli and K. pneumoniae: risk factors for multidrug-resistance BJID
Serefhanoglu,Kivanc; Turan,Hale; Timurkaynak,Funda Ergin; Arslan,Hande.
This prospective case-control study was conducted from October 2003 to June 2007 to evaluate risk factors for multidrug resistance among extended-spectrum-b-lactamase-producing Escherichia coli and Klebsiella spp. (ESBL-EK) isolates in blood cultures. All adult patients (>18 years old) whose blood cultures grew ESBL-EK during the study period were included. An ESBL-EK isolate was defined as MDR if it was resistant to at least one member of following two classes of antibiotics: aminoglycosides (amikacin, gentamicin, or netilmycin) and fluoroquinolones (ofloxacin, or ciprofloxacin). Case patients were those with a MDR ESBL-EK isolate, and control patients were those with a non-MDR ESBL-EK isolate. A total of 94 bloodstream infections, including 37 (39,4%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ESBLs; Multidrug resistance; Risk factors.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000600003
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Candida albicans Clinical Isolates from a Southwest Brazilian Tertiary Hospital Exhibit MFS-mediated Azole Resistance Profile Anais da ABC (AABC)
PINTO,ANA CAROLINA C.; ROCHA,DEBORA A.S.; MORAES,DANIEL C. DE; JUNQUEIRA,MARIA L.; FERREIRA-PEREIRA,ANTONIO.
Abstract: Candida albicans is the most frequent fungal species that causes infections in humans. Fluconazole is the main antifungal used to treat Candida infections, and its prolonged and indiscriminate use for the last decades are the most established causes which originated resistant strains. Fungal drug resistance is associated to alterations in ERG11 gene and overexpression of multidrug resistance (MDR) transporters belonging to two families: ATP-binding cassette (ABC) and Major Facilitator Superfamily (MFS). To evaluate the role of MFS transporters in azoles resistance of C. albicans clinical strains, this study aimed to analyze four Candida albicans clinical isolates from the University Hospital in Juiz de Fora (Minas Gerais/Brazil), selected in our...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida; Fluconazole; MFS; Multidrug resistance; Yeast.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500603
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CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant animal model in vivo BJMBR
Wang,X.M.; Gao,S.J.; Guo,X.F.; Sun,W.J.; Yan,Z.Q.; Wang,W.X.; Xu,Y.Q.; Lu,D..
Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a treatment for drug-resistant breast cancer and to investigate the effect of CIAPIN1 on the drug resistance of breast cancer in vivo. We used lentivirus-vector-based RNAi to knock down CIAPIN1 in nude mice bearing MDR breast cancer tumors and found that lentivirus-vector-mediated silencing of CIAPIN1 could efficiently and significantly inhibit tumor growth when combined with chemotherapy in vivo. Furthermore, Western blot analysis showed that both CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CIAPIN1 gene; Multidrug resistance; RNA interference; MDR1 gene; Breast neoplasms.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400273
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Detection of resistance genes and evaluation of water quality at zoo lakes in Brazil Ciência Rural
Faria,Ana Carolina Silva de; Godoy,Isabela de; Sanches,Anderson Aparecido Amorim; Iglesias,Gabriela Accardi; Candido,Stefhano Luiz; Paz,Regina Célia Rodrigues da; Nakazato,Luciano; Dutra,Valéria.
ABSTRACT: The investigation of the presence of antibiotic-resistance genes in aquatic environments is important to identify possible reservoirs of resistant microorganisms that could be a threat to human and animal health. The aims of this study were to analyze the presence of genes conferring resistance to antimicrobials in the aquatic environment and to assess the quality of water in zoo lakes. Results showed a pattern of genes conferring resistance to multiple antibiotics and turbidity, which was expected to be due to the presence of contaminants. The most frequent genes were sul I and sul II (sulfonamides), which were present in all the lakes, followed by genes encoding β-lactamases such as blaPSE I (77.8%) and ampC (66.7%). However, tet(K), tet(M),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multidrug resistance; Zoo pollution; Aquatic environment; Enterobacteriaceae.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500860
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Determination of multidrug resistance mechanisms in Clostridium perfringens type A isolates using RNA sequencing and 2D-electrophoresis BJMBR
Ma,Yu-Hua; Ye,Gui-Sheng.
In this study, we screened differentially expressed genes in a multidrug-resistant isolate strain of Clostridium perfringens by RNA sequencing. We also separated and identified differentially expressed proteins (DEPs) in the isolate strain by two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). The RNA sequencing results showed that, compared with the control strain, 1128 genes were differentially expressed in the isolate strain, and these included 227 up-regulated genes and 901 down-regulated genes. Bioinformatics analysis identified the following genes and gene categories that are potentially involved in multidrug resistance (MDR) in the isolate strain: drug transport, drug response, hydrolase activity, transmembrane transporter,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium perfringens; Multidrug resistance; RNA sequencing; 2D-electrophoresis; Molecular mechanism.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800609
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Expression of mdr isoforms in mice during estrous cycle and under hormone stimulation Genet. Mol. Biol.
Schiengold,Marion; Schwantes,Lavínia; Ribeiro,Maria F.; Lothhammer,Nívia; Gonzalez,Tatiana P.; Chies,Jose Artur Bogo; Nardi,Nance B..
The multidrug resistance (MDR) phenotype is associated with the expression of P-glycoprotein (Pgp), coded by the multigenic mdr family. Mice present the isoforms mdr1 and mdr3, which are responsible for multidrug resistance, and mdr2, that is involved in the transport of phospholipids. mdr1 expression has more recently been associated also with the secretion of steroid hormones. This work presents an RT-PCR analysis of the expression of mdr isoforms, in several organs of mice during different phases of the estrous cycle. Additionally, females were ovariectomized, submitted to different hormone treatments, and their uterus was analyzed for the expression of mdr isoforms. The results show that in the adrenal gland and ovaries mdr1 is the main isoform during...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adrenal gland; Multidrug resistance; Ovaries; Steroids; Uterus.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400029
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Factors influencing the early mortality in haematological malignancy patients with nosocomial Gram negative bacilli bacteraemia: a retrospective analysis of 154 cases BJID
Metan,Gökhan; Demiraslan,Hayati; Kaynar,Leyla Gül; Zararsız,Gökmen; Alp,Emine; Eser,Bülent.
BACKGROUND: The aim of this study is to assess the factors influencing the early mortality (7- day after index blood culture) in haematological malignancy patients with Gram negative bacilli (GNB) bacteraemia. METHODS: Infection control committee records were reviewed to identify the cases between March 2006 and June 2011. Only one bacteraemic episode per patient was included in the study. RESULTS: A total of 154 patients with GNB bacteraemia were identified. The early mortality rate was 19.5% (30 out of 154). Blood cultures revealed Enterobacteriacea in 120 patients (Escherichia coli; 86, Klebsiella spp.; 28, Enterobacter cloacea; 6) and glucose non-fermenting GNB in 34 patients (Pseudomonas aeruginosa; 15, Acinetobacter baumannii; 11, Stenotrophomonas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haematological malignancy; Bacteraemia; Gram negative bacilli; Mortality; Extended-spectrum beta-lactamase; Intensive care unit; Multidrug resistance.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200005
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Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity BABT
Santos,Neyliane Gonçalves dos; Amaral,Ademir; Cavalcanti,Mariana Brayner; Neves,Maria Amélia Batista; Machado,Cíntia Gonsalves de Faria.
The aim of this work was to establish a protocol to evaluate ionizing radiation effects on P-glycoprotein (P-gp) activity. For this, human peripheral blood samples were irradiated in vitro with different doses and P-gp activity was analyzed for CD4 and CD8 T lymphocytes through rhodamine123-efflux assay by flow cytometry. By simultaneous employment of percentage and mean fluorescence index parameters, subject-by-subject analysis pointed out changes in P-gp activity for some individuals and irradiated samples. Based on this work, the proposed protocol was considered adequate for evaluating P-gp activity on cells after radioactive stress. Besides, this research suggests that P-gp activity could be an important factor to define patient-specific protocols in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Combined therapy; Radiation effect; P-glycoprotein; Multidrug resistance; Functional analysis; Flow cytometry.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700018
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Genotypic and phenotypic detection of efflux pump in Rhodococcus equi BJM
Gressler,Letícia Trevisan; Vargas,Agueda Castagna de; Costa,Mateus Matiuzzi da; Pötter,Luciana; Silveira,Bibiana Petri da; Sangioni,Luis Antônio; Botton,Sônia de Avila.
The req_39680 gene, associated to a putative efflux system, was detected in 60% (54/90) of R. equi isolates by PCR. The phenotypic expression of efflux mechanism was verified in 20% of the isolates using ethidium bromide. For the first time, the expression of efflux mechanism was demonstrated in R. equi.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethidium bromide; Efflux pump; Multidrug resistance; Rhodococcus equi; VapA.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200040
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Identification of microRNAs and mRNAs associated with multidrug resistance of human laryngeal cancer Hep-2 cells BJMBR
Yin,Wanzhong; Wang,Ping; Wang,Xin; Song,Wenzhi; Cui,Xiangyan; Yu,Hong; Zhu,Wei.
Multidrug resistance (MDR) poses a serious impediment to the success of chemotherapy for laryngeal cancer. To identify microRNAs and mRNAs associated with MDR of human laryngeal cancer Hep-2 cells, we developed a multidrug-resistant human laryngeal cancer subline, designated Hep-2/v, by exposing Hep-2 cells to stepwise increasing concentrations of vincristine (0.02-0.96'µM). Microarray assays were performed to compare the microRNA and mRNA expression profiles of Hep-2 and Hep-2/v cells. Compared to Hep-2 cells, Hep-2/v cells were more resistant to chemotherapy drugs (∼45-fold more resistant to vincristine, 5.1-fold more resistant to cisplatin, and 5.6-fold more resistant to 5-fluorouracil) and had a longer doubling time (42.33±1.76 vs 28.75±1.12'h,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multidrug resistance; Laryngeal cancer; Hep-2 cells; MRNA; MicroRNA.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000600546
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Impact of fungal drug transporters on fungicide sensitivity, multidrug resistance and virulence. Repositório Alice
WAARD, M. A. de; ANDRADE, A. C.; HAYASHI, K.; SCHOONBEEK, H-J.; STERGIOPOULOS, I.; ZWIERS, L-H..
bitstream/item/178020/1/ID-27496-1.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Transportadores ABC; ATP-binding cassette transporters; Fungicidas; Multidrug resistance; Aspergillus Nidulans; Botrytis Cinerea; Mycosphaerella graminicola.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/187861
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Isolation of extraintestinal pathogenic Escherichia coli from diarrheic dogs and their antimicrobial resistance profile BJM
Paula,Cleber Jacob Silva de; Marin,José Moacir.
From January to December 2006, 92 Escherichia coli isolates from 25 diarrheic dogs were analyzed by screening for the presence of adhesin-encoding genes (pap, sfa, afa), hemolysin and aerobactin genes. Virulence gene frequencies detected in those isolates were: 12% pap, 1% sfa, 10% hemolysin and 6.5% aerobactin. Ten isolates were characterized as extraintestinal pathogenic E. coli (ExPEC) strains; all showed a multidrug resistance phenotype that may represent a reason for concern due the risk of dissemination of antimicrobial resistant genes to the microbiota of human beings.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; ExPEC; Dog; Antimicrobial resistance; Multidrug resistance.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300016
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Molecular characterization of multidrug-resistant Klebsiella pneumoniae isolates BJM
Hou,Xiang-hua; Song,Xiu-yu; Ma,Xiao-bo; Zhang,Shi-yang; Zhang,Jia-qin.
Klebsiella pneumoniae is an important cause of healthcare-associated infections worldwide. Selective pressure, the extensive use of antibiotics, and the conjugational transmission of antibiotic resistance genes across bacterial species and genera facilitate the emergence of multidrug-resistant (MDR) K. pneumoniae. Here, we examined the occurrence, phenotypes and genetic features of MDR K. pneumoniae isolated from patients in intensive care units (ICUs) at the First Affiliated Hospital of Xiamen University in Xiamen, China, from January to December 2011. Thirty-eight MDR K. pneumoniae strains were collected. These MDR K. pneumoniae isolates possessed at least seven antibiotic resistance determinants, which contribute to the high-level resistance of these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Multidrug resistance; Molecular characterization.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300759
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Molecular epidemiology of 16S rRNA methyltransferase in Brazil: RmtG in Klebsiella aerogenes ST93 (CC4) Anais da ABC (AABC)
PASSARELLI-ARAUJO,HEMANOEL; PALMEIRO,JUSSARA K.; MOHARANA,KANHU C.; PEDROSA-SILVA,FRANCISNEI; DALLA-COSTA,LIBERA M.; VENANCIO,THIAGO M..
Abstract Aminoglycosides are a class of antibiotics that play a key role in antimicrobial treatment of Multidrug resistant (MDR) Gram-negative bacilli, typically in combination with β-lactams. Ribosomal 16S RNA modification by methyltransferases (e.g. RmtG) is an aminoglycoside resistance mechanism that, along with the occurrence carbapenem-resistant Enterobacteriaceae (CRE), has become a clinical concern. In Brazil, rmtG genes were initially reported in Klebsiella pneumoniae, and monitoring isolates from other species carrying this gene is critical for epidemiological studies and to prevent dissemination. Here we report the presence of rmtG in Klebisella aerogenes D3 and characterize its genetic context in comparison to isolates from other species....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella aerogenes; Aminoglycoside; 16S rRNA methyltransferases; Multidrug resistance.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000200602
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Multidrug resistance in tumour cells: characterisation of the multidrug resistant cell line K562-Lucena 1 Anais da ABC (AABC)
RUMJANEK,VIVIAN M.; TRINDADE,GILMA S.; WAGNER-SOUZA,KAREN; MELETTI-DE-OLIVEIRA,MICHELE C.; MARQUES-SANTOS,LUIS F.; MAIA,RAQUEL C.; CAPELLA,MÁRCIA A. M..
Multidrug resistance to chemotherapy is a major obstacle in the treatment of cancer patients. The best characterised mechanism responsible for multidrug resistance involves the expression of the MDR-1 gene product, P-glycoprotein. However, the resistance process is multifactorial. Studies of multidrug resistance mechanisms have relied on the analysis of cancer cell lines that have been selected and present cross-reactivity to a broad range of anticancer agents. This work characterises a multidrug resistant cell line, originally selected for resistance to the Vinca alkaloid vincristine and derived from the human erythroleukaemia cell K562. This cell line, named Lucena 1, overexpresses P-glycoprotein and have its resistance reversed by the chemosensitisers...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multidrug resistance; Leukaemia; P-glycoprotein; Chemosensitisers.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000100007
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Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis Biol. Res.
Ortiz-Severín,Javiera; Varas,Macarena; Bravo-Toncio,Catalina; Guiliani,Nicolás; Chávez,Francisco P.
BACKGROUND: Pseudomonas aeruginosa is known to be a multidrug resistant opportunistic pathogen. Particularly, P. aeruginosa PAO1 polyphosphate kinase mutant (ppk1) is deficient in motility, quorum sensing, biofilm formation and virulence FINDINGS: By using Phenotypic Microarrays (PM) we analyzed near 2000 phenotypes of P. aeruginosa PAO1 polyP kinase mutants (ppk1 and ppk2). We found that both ppk mutants shared most of the phenotypic changes and interestingly many of them related to susceptibility toward numerous and different type of antibiotics such as Ciprofloxacin, Chloramphenicol and Rifampicin CONCLUSIONS: Combining the fact that ppk1 mutants have reduced virulence and are more susceptible to antibiotics, polyP synthesis and particularly PPK1, is a...
Tipo: Journal article Palavras-chave: Antibiotic susceptibility; Polyphosphate kinase; Pseudomonas; Phenome; Phenotypic microarrays; Multidrug resistance.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100022
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Nasal carriage of resistant Staphylococcus aureus in a medical student community Anais da ABC (AABC)
GUSHIKEN,CAROLINA Y.; MEDEIROS,LILIANE B.; CORREIA,BRUNA P.; SOUZA,JOYCE M.; MORIS,DANIELA V.; PEREIRA,VALERIA C.; GIUFFRIDA,ROGERIO; RODRIGUES,MARCUS V.P..
ABSTRACT Staphylococcus aureus can cause a variety of infections due to its high transmissibility, high pathogenic potential and resistance to multiple drugs, factors that contribute to the relevance of infections in healthcare services. The aim of this study was to document phenotypic and genotypic resistance factors of Staphylococcus aureus strains, isolated from nasal mucosa of medical students. A nasal swab was collected from the nares (nostrils) of 222 medical students. After collection, the samples were submitted to isolation and identification procedures. From 204 valid samples, 20.6% (42 samples) were positive for S. aureus. For the assessment of phenotypic resistance by disk-diffusion technique, from 42 samples, 95.2% showed resistance to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multidrug resistance; Colonization; MLSB resistance; MRSA.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401501
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