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Bioquímica muscular, maciez da carne e melhoramento das raças zebuínas. Repositório Alice
GONDIM, F..
A baixa maciez da carne zebuína restringe seu acesso a importantes mercados. Tal característica é atribuída a uma maior atividade muscular da calpastatina na carne de zebu do que na de gado taurino. Essa proteína interfere no processo de proteólise post mortem, prejudicando o amaciamento da carne. Existem diferenças bioquímicas entre os tipos de fibras que constituem 95% da massa muscular. A composição percentual das fibras oxidativas e glicolíticas influencia a qualidade das carnes e explica sua variação. Inovações tecnológicas contribuem para a melhoria da qualidade da carne, e conhecer a tipologia muscular do gado zebu é indispensável para entender as razões de sua ?dureza?. Incentivos aplicados à produção de carne zebuína de qualidade contribuem para o...
Tipo: Artigo de periódico Palavras-chave: Cotas Hilton; Fibra muscular; Pecuária de corte; Rastreabilidade bovina; Gado zebu; Gado de corte; Muscle fibers.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/981011
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Correlations Between Muscle Fibers Characteristics and Meat Quality Attributes of Biceps Femoris Muscle: a Comparative Study of 2 Distinctive Broiler Breeds Rev. Bras. Ciênc. Avic.
Mehmood,W; Zhang,C.
ABSTRACT The current study was conducted to develop the correlations between muscle fiber characteristics and meat quality attributes in the biceps femoris muscle of Arbor Acres (AA) and Yellow-feathered chicken (YFC). A total of fourty pure breed birds of AA (n=20) and YFC (n=20) were used in the experiment. After slaughtering at their respective market age of slaughtering: AA 40 d and YFC 120 d, samples were collected for meat quality attributes and myosin ATPase staining for fiber types analyses. Meat quality attributes and muscle fiber characteristics i-e; diameter, cross-sectional area (CSA) and density of AA were significantly different from the YFC (p<0.05). Type I fibers number percentage was significantly higher in YFC than AA, whereas CSA and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Muscle fibers; Arbor Acres; Yellow-feathered chicken; Meat quality; Correlations.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000400300
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Creatine supplementation: effects on blood creatine kinase activity responses to resistance exercise and creatine kinase activity measurement BJPS
Machado,Marco; Pereira,Rafael; Sampaio-Jorge,Felipe; Knifis,Franz; Hackney,Anthony.
The purpose of this study was to determine the effects of creatine supplementation and exercise on the integrity of muscle fiber, as well as the effect of the supplementation on the creatine kinase (CK) assay measurement. Forty-nine sedentary individuals participated in a double-blind study and were divided into two groups: C (n=26) received 4x5-day packages of 0.6 g.kg-1 of body weight contained 50% of creatine + 50% of dextrose, and P (n=23) received packages containing only dextrose. On the first day the groups performed a 1RM test for bench press, seated row, leg extension, leg curl and leg press. On D7 they received the supplements. On the fourteenth day, they performed a training session of five exercises, each in three sets of ten repetitions at 75%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Physical exercise/effects; Creatine/supplementation/effects; Creatine Kinase/assay; Muscle fibers.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000400020
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Desempenho e características histoquímicas do tecido muscular esquelético de cordeiras terminadas em diferentes sistemas - DOI: 10.4025/actascianimsci.v31i4.6410 Animal Sciences
Santello, Graziela Aparecida; UFAM; Macedo, Francisco de Assis Fonseca de; UEM; Dias, Fabio Jacobs; UNEMAT; Mexia, Alexandre Agostinho; UNEMAT; Macedo, Rosa Maria Gomes; CESUMAR; Lourenço, Fábio José; CESUMAR.
Verificou-se o sistema de terminação de 44 cordeiras ½ Dorset Santa Inês na freqüência, diâmetro de fibras musculares esqueléticas e desempenho. Amostras do músculo semitendinosus foram coletadas para análise do diâmetro, frequência e efeito de modulação das fibras musculares do desmame e abate. As cordeiras foram divididas em dois sistemas de terminação. Pastagem com suplementação (1,5% PV) com 27,01% PB e 4,51 Mcal de EB kg-1 MS e no confinamento receberam ração (4% PV) com 15,56% de PB e 4,32 Mcal de EB kg-1 MS. O ganho de peso médio diário (GPMD) foi de 0,157 e 0,148 kg para confinamento (CT) e pastagem mais suplementação (PS), respectivamente. Os diâmetros das fibras SO, FOG e FG para CT e PS, foram, respectivamente, 29,04 e 28,55, 34,46 e 35,80 e...
Palavras-chave: Desempenho; Fibras musculares; Modulação de fibras Nutrição e Alimentação Animal performance; Muscle fibers; Modulation fibers.
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/6410
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Effect of salbutamol on innervated and denervated rat soleus muscle BJMBR
Šoic-Vranic,T.; Bobinac,D.; Bajek,S.; Jerkovic,R.; Malnar-Dragojevic,D.; Nikolic,M..
The objective of the present investigation was to perform a 14-day time-course study of treatment with salbutamol, a ß2 adrenoceptor agonist, on rat soleus muscle in order to assess fiber type selectivity in the hypertrophic response and fiber type composition. Male Wistar rats were divided into four groups: control (N = 10), treated with salbutamol (N = 30), denervated (N = 30), and treated with salbutamol after denervation (N = 30). Salbutamol was injected intraperitoneally in the rats of the 2nd and 4th groups at a concentration of 0.3 mg/kg twice a day for 2 weeks. The muscles were denervated using the crush method with pean. The animals were sacrificed 3, 6, 9, 12, and 14 days after treatment. Frozen cross-sections of soleus muscle were stained for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salbutamol; Rat skeletal muscle; Soleus muscle; Denervation; Histochemistry; Muscle fibers.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005001200008
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Evaluation of autolysis induced histologic alterations in skeletal muscle tissue of non-traditional meat animals Ciênc. Tecnol. Aliment.
KAIMBAYEVA,Leila; KENENBAY,Shynar; ZHANTLEUOV,Daniyar; TAYEVA,Aigul; AKHMETOVA,Nursulu; MEDEUBAYEVA,Zhanar.
Abstract Raw meat is characterized by various natural cellular processes that lead to its chemical content alteration. The purpose of the present study is to evaluate autolysis induced histologic alterations in skeletal muscle tissue of cow elk. In this study the authors used skeletal muscle tissue of 2-year-old cow elk. The tissue was sampled from m. quadriceps femoris. The study results showed that in 48 hours post mortem linear striation in muscle tissues was expressed. But 72 hours post mortem the integrity of muscle fibers degraded and they became inconsistently stained. The obtained data showed that post mortem alterations were characterized by significant degradation and separation of muscle fibers and nuclei and nuclear structures lysis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Elk meat; Post mortem aging; Muscle fibers; Nuclei lysis; Sarcolemma.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019005031121
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Fiber Type Composition in Semitendinous Muscle of Wistar Rats and Effects of Intermittent Training on its Hypertrophy International Journal of Morphology
Ferreira,Alexandre; Campos,Gerson Eduardo Rocha.
Skeletal muscles respond to several stimuli changing their phenotype. Muscular fibers adaptation capability is related to the presence of several myosin heavy chains (MHC). These express four types of pure fibers: I, IIA, IID and IIB containing MHCI, IIa, IId and IIb, respectively. Among pure fibers, there are hybrid fibers, which can express two or more types of myosins. In this study, types of fibers constituting male Wistar rats semitendinous and their myosin heavy chains, as well as influence of intermittent training on hypertrophy of these fibers have been checked through MATPase histochemical technique and electrophoretic proteins separation. All types of pure and hybrid muscular fiber have been found, however the fibers of the types IIA, IID and IIB...
Tipo: Journal article Palavras-chave: Semitendinous muscle; Muscle fibers; Intermittent training; Hypertrophy.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000100010
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HIGH RESOLUTION SEM OF INTRINSIC MUSCLE FIBERS OF ANTERIOR THIRD RET'S TONGUE International Journal of Morphology
Motoyama,Aracy A.; Watanabe,Ii-sei; Ogawa,Koichi.
The characteristics of intrinsic muscle fibers in the anterior third of the adult rat tongue were studied employing light microscopy, SEM and high-resolution SEM (HRSEM) methods. For light microscopy, specimens were fixed in Bouin's solution and embedded in paraffin. In order to identify the muscle fibers and bundles of collagen fibers, frontal sections were stained with hematoxylin-eosin, azo-carmin and picro-sirius. The results showed that muscle fibers near the lamina propria are fixed with connective tissue, constituting several groups. The bundles of muscle fibers are arranged in longitudinal, vertical and transversal directions. For SEM, specimens were fixed in modified Karnovsky solution and freeze-fractured in liquid nitrogen revealed the collagen...
Tipo: Journal article Palavras-chave: Muscle fibers; Tongue; Rat; HRSEM; Mitochondria; Basement membrane.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000400006
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Histochemical and Morphological Characteristics of the Levator Ani Muscle in Rats International Journal of Morphology
do Carmo,Edna Maria; Miyaoka,Tatiana Midori; da Silva,Natália Denipoti; de Camargo,Marcela Regina; Rocha,Ana Paula Rodrigues; Pelai,Eliza Bizetti; Figueira,Jéssica Ibde Jaquiel; Fregonesi,Cristina Elena Prado Teles; Sato,José Takaiti; de Machado,Roberto Gameiro; de Souza,Wilson Machado.
The current study aimed to analyze the histochemical and morphological characteristics of the levator ani muscle in rats. For this, we used 10 Wistar rats (5 males and 5 females), weighing between 200 and 765g. The animals were dissected fresh and in formalin for the levator ani muscle anatomical observation. Muscle fragments were collected and frozen in n-Hexane previously cooled in liquid nitrogen. Then, the muscles were transferred to a microtome cryostat (HM 505 E Microm), being fixed in metal mounts with the adhesive Tissue Freezing Medium. Histological sections of 6.0µm were removed and subjected to HE staining. Other sections were subjected to NADH-TR and SDH reactions. After being dissected and fixed, the architecture of the female pelvic floor...
Tipo: Journal article Palavras-chave: Pelvic floor; Muscle skeletal; Physical therapy; Model animals; Muscle fibers.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400021
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Influence of Reduced Salinity on the Histochemical Fiber Type Composition of the Claw Closer Muscle of Two Estuarine Crab Species, Cyrtograpsus angulatus and Neohelice granulata (Decapoda: Varunidae) International Journal of Morphology
Longo,María Victoria; Díaz,Alcira Ofelia.
The estuarine crustaceans are exposed to frequent and abrupt environmental salinity changes that involve adjustments at different levels in muscle tissue. The histochemical composition of fiber types of the claw closer muscle of Cyrtograpsus angulatus and Neohelice granulata (Dana, 1851) under hyper regulatory conditions was analyzed. Cryosections of the muscle were treated with myosin-adenosine triphosphatase (m-ATPase), succinic dehydrogenase (SDH), periodic acid Schiff (PAS) and Sudan Black B. The mean diameters, the relative area and the proportion of each muscle fiber type were calculated. Types I and IV would belong to 'extreme' groups, whereas types II and III would be considered 'intermediate'. Type I fibers were large and exhibited a weak reaction...
Tipo: Journal article Palavras-chave: Euryhaline crabs; Histochemistry; Hyper regulation; Muscle fibers; Salinity stress.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200025
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MORPHOLOGICAL ALTERATIONS IN THE MASSETER MUSCLE INDUCED BY UNILATERAL EXTRACTION OF THE UPPER MOLARS IN GUINEA PIGS (Cavia porcellus) International Journal of Morphology
Mardegan Issa,João Paulo; Mizusaki Iyomasa,Mamie; Ioshie Mizusaki,Cristina; Watanabe,Ii-sei; Mandarim de Lacerda,Carlos Alberto.
The objective of the present study was to analyze morphological aspects of the structures of the stomatognathic system and their relationship with malocclusion. For this purpose, we determined the morphological alterations that occur in the masseter muscle of guinea pigs (Cavia porcellus) after the induction of malocclusion by extraction of the left upper molars. Animals were divided into two groups (malocclusion and control group) and submitted to macroscopic, biometric and light microscopic analyses. The results showed a reduction in the width of the masseter muscle mainly in left side in the malocclusion group 60 days after unilateral extraction. Microscopically, round and polygonal muscle fibers of several diameters were observed, and altered muscle...
Tipo: Journal article Palavras-chave: Masseter muscle; Morphology; Light microscopy; Muscle fibers; Guinea pig.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000300009
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Muscular Partitioning in the Semitendinosus Muscle of the Pig International Journal of Morphology
Graziotti,Guillermo H; Ríos,Clara M; Rodríguez Menéndez,José M; Salinas,Matías A; Bosco,Alexis; Paltenghi Ceschel,Alejandra; Affricano,Néstor O; Victorica,Carlos L.
Neuromuscular compartments are subvolumens supplied by a primary nerve branch with homogeneous fiber characteristics. This muscular organization is important for understanding the muscle function in relation to postural and dynamic implications and also is relevant for appraising meat quality. Current researches associate fiber muscle characteristics with perimortem processes that regulate muscle transformation into meat. It is known that pig muscle has four heavy chain myosin isoforms: I, IIa, IIx and IIb. Castrated male pigs were slaughtered at 100 kg live weight. Semitendinosus muscle samples of each subvolumen determined previously (R1, R2, R3, R4) were frozen in liquid nitrogen and reacted for myofibrillar ATPase and NADH-TR for determining fiber...
Tipo: Journal article Palavras-chave: Pig; Semitendinosus muscle; Muscle fibers; Meat quality.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000300048
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