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| Ramos,Daniela Fernandes; Matthiensen,Alexandre; Colvara,Wilson; Votto,Ana Paula Souza de; Trindade,Gilma Santos; Silva,Pedro Eduardo Almeida da; Yunes,João Sarkis. |
| Background The present work aimed to evaluate the antimycobacterial activity and cytotoxicity of Microcystis aeruginosa toxins, the MC-LR variant and purified extract of [D-Leu1] microcystin-LR. Methods The antimicrobial activity of M. aeruginosa extract and microcystin was evaluated by resazurin microtiter assay against Mycobacterium tuberculosis, M. terrae, M. chelonae and M. kansasii. The cytotoxicity assay was performed by trypan blue exclusion against the HTC cell line. Results Antimicrobial activity was observed in the hexanic extract of M. aeruginosa (RST 9501 strain) against M. tuberculosis, including sensitive and resistant strains with minimal inhibitory concentrations (MIC) between 1.93 μM and 0.06 μM. The high activity of M. aeruginosa hexanic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacteria; Antimycobacterial agents; Cytotoxic activity; Microcystins. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100315 |
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| García-Agudo,Lidia; Jesús,Iría; Rodríguez-Iglesias,Manuel; García-Martos,Pedro. |
| A total of 54 rapidly growing mycobacteria (RGM) isolated from patients attended in the two hospitals of Cádiz Bay (Spain) were selected during a seven-year-period (2000-2006) in order to evaluate the INNO-LiPA Mycobacteria v2 assay for mycobacterial identification, based on the reverse hybridization principle. The strains were cultured in Löwenstein-Jensen and Middlebrook 7H9 media and identified to the species level by sequencing of the 16S rRNA, PCR-restriction enzyme analysis of the hsp65 gene, conventional tests and INNO-LiPA Mycobacteria v2 assay. By the molecular methods we identified a total of 12 different species: 23 Mycobacterium fortuitum, 11 M. chelonae, 10 M. abscessus, 2 M. senegalense, 1 M. alvei, 1 M. brumae, 1 M. mageritense, 1 M.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacteria; Rapidly growing mycobacteria; M. fortuitum; M. chelonae; M. abscessus. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300048 |
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| Brunetta,Denise Menezes; De Santis,Gil Cunha; Vilar,Fernando Crivelenti; Brandão,Renata Amorim; Muniz,Renata Zomer de Albernaz; Lima,Geovana Momo Nogueira de; Amorelli-Chacel,Manuela Emiliana; Covas,Dimas Tadeu; Machado,Alcyone Artioli. |
| HIV patients infected through injected drug use have poorer prognosis than other groups. We evaluated the hematological alterations and rates of co-infections in injected drug use patients with AIDS. Injected drug use patients were younger, predominantly of male gender, and presented lower CD4, total lymphocyte, and platelet counts, but not neutrophil count, than control group. Injected drug use patients had a higher rate of hepatitis C and mycobacteria infection. Furthermore, all injected drug use patients with hemoglobin <10.0 g dL-1 and lymphocyte <1000 µL-1 had CD4 count lower than 100 µL-1. In conclusion, HIV-infected injected drug use patients constitute a special group of patients, and hemoglobin concentration and lymphocyte count can be used... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AIDS; Injected drug use; Anemia; HCV; Mycobacteria. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600005 |
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| Parise-Fortes,Maria Rita; Lastória,Joel Carlos; Marques,Silvio Alencar; Putinatti,Maria Stella Ayres; Stolf,Hamilton Ometto; Marques,Mariângela Ester Alencar; Haddad,Vidal. |
| Leprosy is a chronic infectious disease caused by Mycobacterium leprae, a microorganism that usually affects skin and nerves. Although it is usually well-controlled by multidrug therapy (MDT), the disease may be aggravated by acute inflammatory reaction episodes that cause permanent tissue damage particularly to peripheral nerves. Tuberculosis is predominantly a disease of the lungs; however, it may spread to other organs and cause an extrapulmonary infection. Both mycobacterial infections are endemic in developing countries including Brazil, and cases of coinfection have been reported in the last decade. Nevertheless, simultaneous occurrence of perianal cutaneous tuberculosis and erythema nodosum leprosum is very rare, even in countries where both... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Mycobacteria; Leprosy; Tuberculosis; Erythema nodosum leprosum. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200506 |
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| Gadelha,Ângela; Accácio,Náurea; Grinzstejn,Beatriz; Veloso,Valdiléa; Silveira,Liane Braga da; Fandinho,Fátima; Saad,Maria Helena; Lourenço,Maria Cristina; Rolla,Valeria. |
| OBJECTIVE: Evaluate the incidence of mycobacterial disease and the colonization of the respiratory and gastrointestinal tracts by Mycobacterium avium complex (MAC) bacteria in AIDS patients. METHODS: Inclusion criteria: HIV-positive individuals with at least one CD4+ count < 100 cells/mm³. Exclusion criteria: Mycobacterial disease and MAC prophylaxis. Stool, sputum, and blood cultures were prospectively obtained every month from September, 1997, to December, 1999. The incidence was calculated using Poisson regression. Survival was estimated by the Kaplan Meier method and the Cox proportional hazard model. RESULTS: We followed-up 79 patients during a median period of 428 days. Blood cultures (n = 742) were negative for all mycobacteria. Positive cultures... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DMAC; Colonization; AIDS; Mycobacteria. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000500006 |
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| Monego,Fernanda; Duarte,Rafael Silva; Nakatani,Sueli Massumi; Araújo,Wildo Navegantes; Riediger,Irina Nastassja; Brockelt,Sonia; Souza,Verena; Cataldo,Jamyra Iglesias; Dias,Rubens Clayton da Silva; Biondo,Alexander Welker. |
| OBJECTIVE: One hundred thirty-one cases of postsurgical infections were reported in Southern Region of Brazil between August 2007 and January 2008. Thirty-nine (29.8%) cases were studied; this report describes epidemiological findings, species identification, antimicrobial susceptibility and clonal diversity of rapidly growing mycobacteria isolated in this outbreak. METHODS: All 39 isolates were analyzed by Ziehl-Nielsen stained smear, bacterial culture and submitted to rpoB partial gene sequencing for identification. The isolates were also evaluated for their susceptibility to amikacin, cefoxitin, clarithromycin, ciprofloxacin, doxycycline, tobramycin and sulfamethoxazole. RESULTS: Thirty-six isolates out of the confirmed cases were identified as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacteria; Atypical mycobacterium infections microbiological analysis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500004 |
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| Honarvar,Behnam; Movahedan,Hossein; Mahmoodi,Mojtaba; Sheikholeslami,Fatemeh Maryam; Farnia,Parisa. |
| Atypical fast-growing Mycobacterium species are usually identified after laser-assisted in situ keratomileusis, cosmetic surgeries, and catheter-related, pulmonary or soft tissue infections. We herein present the case of a 56-year-old man with purulent discharge, redness, and foreign body sensation in his left eye. He underwent two surgeries that partially controlled the infection but were not curative. Corneal transplantation was performed, and a biopsy of the excised cornea indicated Mycobacterium aurum infection, which was confirmed by polymerase chain reaction-restriction fragment length polymorphism analysis. This appears to be the first documented case of keratitis attributable to the non-tuberculous mycobateria M. aurum. The intractable extra-ocular... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Mycobacteria; Atypical Keratitis Corneal transplantation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200019 |
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| Kargupta,Roli; Puttaswamy,Sachidevi; Lee,Aiden J.; Butler,Timothy E.; Li,Zhongyu; Chakraborty,Sounak; Sengupta,Shramik. |
| Abstract Background: Multiple techniques exist for detecting Mycobacteria, each having its own advantages and drawbacks. Among them, automated culture-based systems like the BACTEC-MGIT™ are popular because they are inexpensive, reliable and highly accurate. However, they have a relatively long "time-to-detection” (TTD). Hence, a method that retains the reliability and low-cost of the MGIT system, while reducing TTD would be highly desirable. Methods: Living bacterial cells possess a membrane potential, on account of which they store charge when subjected to an AC-field. This charge storage (bulk capacitance) can be estimated using impedance measurements at multiple frequencies. An increase in the number of living cells during culture... |
| Tipo: Journal article |
Palavras-chave: Mycobacteria; Tuberculosis; Electrical impedance spectroscopy; BD Bactec MGIT 960; M. bovis BCG; M. smegmatis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100214 |
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| Chagas,M.; Silva,R.M. da; Bazzo,M.L.; Santos,J.I. dos. |
| Early diagnosis plays a vital role in controlling tuberculosis. The conventional methodology is slow, with results taking several weeks, in addition to having low sensitivity, especially in clinical paucibacillary samples. The objective of this study was to evaluate the use of polymerase chain reaction (PCR) on solid medium culture for a rapid diagnosis of tuberculosis, mainly in cases of negative sputum smears. Forty sputum samples were collected from inpatients with tuberculosis treated for less than 2 days. Bacilloscopy, PCR for sputum, culture on Löwestein-Jensen (LJ) solid medium, and daily PCR from culture were performed on each sample. DNA extracted from the BCG vaccine, which contains attenuated bacillus Calmette-Guérin, was used as the positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Mycobacteria; PCR. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000600004 |
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| Registros recuperados: 14 | |
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