SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

The association between HLA specificities and leprosy was investigated in a Southern Brazilian population. One hundred and twenty-one patients and 147 controls were typed for HLA-A, B, Cw, DR and DQ. Patients were subdivided into the following subgroups, according to clinical, histological and immunological criteria: lepromatous (N = 55), tuberculoid (N = 32), dimorphous (N = 20), and indeterminate (N = 14). The frequencies of HLA specificities were compared between the total group of patients and controls, and between the same controls and each subgroup of patients. After correction of the probabilities, deviations were not significant, except for the DR2 specificity, which presented a frequency of 44.2% in the total group of patients and 56.3% in the...

Critical analysis: use of polymerase chain reaction to diagnose leprosy

Palavras-chave: HLA; Leprosy; Mycobacterium leprae.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100008

BJPS

Maltempe,Flaviane Granero; Baldin,Vanessa Pietrowski; Lopes,Mariana Aparecida; Siqueira,Vera Lúcia Dias; Scodro,Regiane Bertin de Lima; Cardoso,Rosilene Fressatti; Caleffi-Ferracioli,Katiany Rizzieri.

ABSTRACT Leprosy is a neglected tropical disease and an important public health problem, especially in developing countries. It is a chronic infectious disease that is caused by Mycobacterium leprae, which has a predilection for the skin and peripheral nerves. Although it has low sensitivity, slit-skin smear (SSS) remains the conventional auxiliary laboratory technique for the clinical diagnosis of leprosy. Polymerase chain reaction (PCR) is a molecular biology technique that holds promise as a simple and sensitive diagnostic tool. In the present study, the performance of two PCR methods, using different targets, PCR-LP and PCR-P, were compared with SSS with regard to leprosy diagnosis in a reference laboratory. M. leprae DNA was extracted from 106 lymph...

Detection of Lsr2 gene of Mycobacterium leprae in nasal mucus

Palavras-chave: Leprosy/clinical diagnosis; Polymerase chain reaction/clinical diagnosis use; Mycobacterium leprae.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100018

BABT

Custodio,Luiz Antonio; Saito,Alexandre; Amarante,Marla Karine; Fujita,Thiago Cezar; Perim,Aparecida de Lourdes; Costa,Ivete Conchon; Felipe,Ionice; Jankevicius,Shiduca Itow.

In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific primers for Lsr2 gene of Mycobacterium leprae. The presence of Lsr2 gene in the nasal mucus was detected in 25.80% of patients with paucibacillari leprosy, and 23.07% of contacts. Despite the absence of clinical features in the contact individuals, it was possible to detect the presence of Lsr2 gene in the nasal mucus of these individuals. Therefore, PCR detection of M. leprae targeting Lsr2 gene using nasal mucus samples could contribute to early diagnosis of leprosy.

Further biochemical characterization of Mycobacterium leprae laminin-binding proteins

Palavras-chave: Mycobacterium leprae; Leprosy; Hanseniase; Nasal mucus; PCR.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300007

Marques,M.A.M.; Mahapatra,S.; Sarno,E.N.; Santos,S.; Spencer,J.S.; Brennan,P.J.; Pessolani,M.C.V..

It has been demonstrated that the alpha2 chain of laminin-2 present on the surface of Schwann cells is involved in the process of attachment of Mycobacterium leprae to these cells. Searching for M. leprae laminin-binding molecules, in a previous study we isolated and characterized the cationic proteins histone-like protein (Hlp) and ribosomal proteins S4 and S5 as potential adhesins involved in M. leprae-Schwann cell interaction. Hlp was shown to bind alpha2-laminins and to greatly enhance the attachment of mycobacteria to ST88-14 Schwann cells. In the present study, we investigated the laminin-binding capacity of the ribosomal proteins S4 and S5. The genes coding for these proteins were PCR amplified and their recombinant products were shown to bind...

Interleukin-10-dependent down-regulation of interferon-gamma response to Leishmania by Mycobacterium leprae antigens during the clinical course of a coinfection

Palavras-chave: Mycobacterium leprae; Laminin; Adhesion; Schwann cell; Ribosomal protein; Histone.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400004

Azeredo-Coutinho,R.B.G.; Matos,D.C.S.; Nery,J.A.C.; Valete-Rosalino,C.M.; Mendonça,S.C.F..

We have described a case of a patient with an intriguing association of mucocutaneous leishmaniasis with lepromatous leprosy, two opposite polar forms of these spectral diseases. In the present follow-up study, we investigated the effect of the addition of Mycobacterium leprae antigens on interferon-gamma (IFN-γ) production in Leishmania antigen-stimulated cultures of peripheral blood mononuclear cells (PBMC) from this patient. For this purpose, PBMC cultures were stimulated with crude L. braziliensis and/or M. leprae whole-cell antigen extracts or with concanavalin A. In some experiments, neutralizing anti-human interleukin (IL)-10 antibodies were added to the cultures. IFN-γ and IL-10 levels in culture supernatants were measured by ELISA. During active...

Leprosy and hepatitis B coinfection in southern Brazil

Palavras-chave: Interferon-gamma; Interleukin-10; Mucocutaneous leishmaniasis; Lepromatous leprosy; Leishmania braziliensis; Mycobacterium leprae.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000700010

Leitão,Cleverson; Ueda,Denis; de Moraes Braga,Anna Carolina; Boldt,Angelica B.W.; Messias-Reason,Iara J.T..

To investigate the association of leprosy with hepatitis B virus (HBV) infection, as yet unknown for South Brazil, we assessed hepatitis B virus coinfection in 199 South Brazilian leprosy patients (119 lepromatous, 15 tuberculoid, 30 borderline, 12 undetermined and 23 unspecified) and in 681 matched blood donors by screening for the hepatitis B virus markers HBSAg and anti-HBc, using ELISA. Positive samples were retested and anti-HBc+ only samples were tested for the hepatitis B surface antibody (anti-HBs). There was a strong association between leprosy and hepatitis B virus infection (OR = 9.8, 95% CI = 6.4–14.7; p = 0.004·E−30), as well as an association between HBV infection and lepromatous leprosy, compared to other forms (OR = 2.4, 95% CI = 1.2–4.8; p...

qPCR detection of Mycobacterium leprae in biopsies and slit skin smear of different leprosy clinical forms

Palavras-chave: Leprosy; Hepatitis B; Leprosarium; HBV; Mycobacterium leprae.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100008

Azevedo,Michelle de Campos Soriani; Ramuno,Natália Mortari; Fachin,Luciana Raquel Vincenzi; Tassa,Mônica; Rosa,Patrícia Sammarco; Belone,Andrea de Faria Fernandes; Diório,Suzana Madeira; Soares,Cleverson Teixeira; Garlet,Gustavo Pompermaier; Trombone,Ana Paula Favaro.

Abstract Leprosy, whose etiological agent is Mycobacterium leprae, is a chronic infectious disease that mainly affects the skin and peripheral nervous system. The diagnosis of leprosy is based on clinical evaluation, whereas histopathological analysis and bacilloscopy are complementary diagnostic tools. Quantitative PCR (qPCR), a current useful tool for diagnosis of infectious diseases, has been used to detect several pathogens including Mycobacterium leprae. The validation of this technique in a robust set of samples comprising the different clinical forms of leprosy is still necessary. Thus, in this study samples from 126 skin biopsies (collected from patients on all clinical forms and reactional states of leprosy) and 25 slit skin smear of leprosy...

Salivary anti-PGL-1 IgM may indicate active transmission of Mycobacterium leprae among young people under 16 years of age

Palavras-chave: Mycobacterium leprae; QPCR; Leprosy; Bacilloscopy.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100071

Macedo,Alexandre Casimiro de; Cunha Jr.,José Evandro; Yaochite,Juliana Navarro Ueda; Tavares,Clodis Maria; Nagao-Dias,Aparecida Tiemi.

Abstract Considering that the main route of Mycobacterium leprae transmission is the upper respiratory tract, detection of salivary antibodies can be a useful tool for diagnosing early infection. The study aimed to analyze salivary anti-PGL-1 IgA and IgM antibodies in 169 children aged 4-16 years old, who lived nearby or inside the house of multibacillary or paucibacillary leprosy patients in two endemic cities in Alagoas State - Brazil. Salivary anti-PGL-1 antibodies were quantified by modified ELISA method. The frequency of contact and clinical form of the index case were significantly associated with salivary antibody levels. High frequency of IgM positivity strongly suggests active transmission of M. leprae in these communities. We suggest in the...

Search for Mycobacterium leprae in wild mammals

Palavras-chave: Leprosy; Salivary antibodies; Phenolic glycolipid-1 antigen; Mycobacterium leprae.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500557

Pedrini,Sílvia Cristina Barboza; Rosa,Patrícia Sammarco; Medri,Ísis Meri; Mourão,Guilherme; Bagagli,Eduardo; Lopes,Carlos Alberto de Magalhães.

Leprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus...

Use of the polymerase chain reaction to detect Mycobacterium leprae in urine

Palavras-chave: Dasypus novemcinctus; Euphractus sexcinctus; Cabassous tatouay; Mycobacterium leprae; Eco-epidemiology; Wild mammals.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100010

Caleffi,K.R.; Hirata,R.D.C.; Hirata,M.H.; Caleffi,E.R.; Siqueira,V.L.D.; Cardoso,R.F..

Leprosy is an infectious disease caused by Mycobacterium leprae. The polymerase chain reaction (PCR) has been applied to detect M. leprae in different clinical samples and urine seems to be attractive for this purpose. PCR was used to improve the sensitivity for diagnosing leprosy by amplifying a 151-bp PCR fragment of the M. leprae pra gene (PCR-Pra) in urine samples. Seventy-three leprosy patients (39 males and 34 females, 14 to 78 years old) were selected for leprosy diagnosis at a reference laboratory in Maringá, PR, Brazil. Of these, 36 were under anti-leprosy multidrug therapy with dapsone and rifampicin for tuberculoid (TT) and dapsone, rifampicin and clofazimine for borderline (BB) and lepromatous (LL) forms. The control group contained 50 healthy...