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Registros recuperados: 16
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Bacteria isolated from abscesses of small ruminants inspected in the semiarid region of Brazil. Repositório Alice
SILVA, W. E. de L. e; GOLVEIA, G. V.; SÁ, M. da C. A. de; GOUVEIA, J. J. de; PEIXOTO, R. de M.; RIET-CORREA, F.; VESCHI, J. L. A.; COSTA, M. M. da.
Loss in the supply chain of small ruminants owing to condemnations of carcasses in the abattoirs and slaughterhouses is common in northeastern Brazil. This study aims to identify bacterial agents, including Mycobacterium spp., in the abscesses found in the postmortem analysis of the carcasses of sheep and goats bred in northeastern Brazil. Our analysis involved 679 goats and 1,838 sheep carcasses. Abscess samples were extracted and inoculated on blood agar and Lowenstein Jensen with pyruvate or glycerol for bacterial isolation. We then performed polymerase chain reaction of the hps 65 gene; samples positive for Mycobacterium spp. were subjected to DNA sequencing. Relative frequencies of abscesses in goats and sheep were 5.44 and 3.26%, respectively....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Inspeção; Isolamento; PCR; Bacteria; Mycobacterium; Caprino; Cadeia produtiva; Ovino; Produção animal; Animal production; Goat; Sheep.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1067340
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Congenital tuberculosis: a case report BJID
Vilarinho,Liana Consuelo Santana.
Congenital tubercolosis is an unusual and severe clinical pattern of tuberculosis presentation of Mycobacterium tuberculosis infection. Furthermore, it usually has a difficult treatment. We report a two-month-old male infant who presented with fever, dyspnea and a diffuse micronodular pattern at x-ray; mother with severe tuberculosis. Treatment with the triple drug regimen was initiated, but the child developed jaundice and an increase in liver enzymes on various occasions during treatment. A regimen specifically developed for cases of intolerance was initiated but there was no improvement in hyperthermia. Finally, the dose of INH and RMP was increased, the fever receded and the child was cured. This case remarks difficulties on diagnosis and therapeutic...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Tuberculosis; Congenital; Antituberculosis medication; Mycobacterium.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500014
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Desenvolvimento de uma vacina de dna contra Corynebacterium pseudotuberculosis. Repositório Alice
GOMES, J. S.; SOARES, C. O.; SANTOS, L. R. dos; SANCHES, S. C.; GALVÃO, C. E.; MELO, P. R; ROSINHA, G. M. S..
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Linfadenite Casesa (LC); Ovinos e caprinos; Perdas econômicas; Mycobacterium.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/942920
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Diagnóstico molecular de Mycobacterium spp. em lesões sugestivas de tuberculose bovina. Repositório Alice
LUIZ, H. L.; ARAUJO, F. R.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; RUSSI, L. S.; FARIAS, T. A.; SOUZA, I. I. F.; ELISEI, C.; ROSINHA, G. M. S.; SOARES, C. O..
O objetivo deste estudo é desenvolver um teste de diagnóstico rápido de reação em cadeia da polimerase (PCR) em tempo real diretamente de lesões post mortem sugestivas de tuberculose, que permita a detecção e diferenciação de micobactérias dos complexos M. tuberculosis, M. avium e outras micobactérias não tuberculosas de interesse médico-veterinário.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Sanidade animal; Tuberculose bovina; Mycobacterium.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/876951
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Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction Rev. Microbiol.
Sircili,Marcelo Palma; Roxo,Eliana; Leão,Sylvia Cardoso.
Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Amplification; PCR; MAC; Mycobacterium; Identification.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200011
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Identification and classification of Mycobacterium by MALDI-TOF mass spectrometry. Repositório Alice
OLARTE, L. C.; DUARTE, L. de F. C.; LEGUIZAMON, G. O. de C.; ARAUJO, F. R.; VERBISCK, N. V..
bitstream/item/166582/1/Identification-and-classification-of-Mycobacterium.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Mycobacterium; MALDI-TOF mass spectrometry; Bovine tuberculosis; Species identification; Mycobacterium bovis; Mycobacterium smegmatis.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079459
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Identification of non-tuberculous mycobacteria from the Central Public Health Laboratory from Mato Grosso do Sul and analysis of clinical relevance BJM
Moraes,Paulo Ricardo de Souza; Chimara,Erica; Telles,Maria Alice da Silva; Ueki,Suely Yoko Misuka; Cunha,Eunice Atsuko Totumi; Honer,Michael Robin; Leão,Sylvia Cardoso.
Non-tuberculous mycobacteria isolated at the Central Public Health Laboratory from Mato Grosso do Sul in 2003 and 2004 were identified by conventional phenotypic methods (TI) and by PCR-Restriction Enzyme Analysis (PRA) using the hsp65 gene as target (PRA-hsp65). With 15 of the 32 analysed isolates, results of both methods were concordant, being 8 Mycobacterium avium, 3 M. fortutium, 1 M. kansasii, 1 M. flavescens, 1 M. peregrinum and 1 Nocardia brasiliensis. TI of 12 isolates was inconclusive. Novel PRA-hsp65 patterns were observed with 11 isolates. Medical data were evaluated for inference of clinical relevance of these isolates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium; PCR; Identification.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200013
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Isolation of Mycobacterium spp. in milk from cows suspected or positive to tuberculosis Braz. J. Vet. Res. Anim. Sci.
PARDO,Renata Bonini; LANGONI,Hélio; MENDONÇA,Lia Jeanne Pereira; CHI,Kung Dahr.
This study was performed considering the public health hazards related to the elimination of mycobacteria through milk of dairy cows suspected or positive for tuberculosis presenting no clinical alterations. A total of 780 milk samples from 52 animals, positive or suspected for tuberculosis, according to Stormont's test, were analysed to detect Mycobacterium spp. The samples consisted of 300 ml/cow, collected in the first milking of the day, during 15 days. Frozen samples were sent to the laboratory, inoculated in Löwenstein-Jensen with reduced glicerol (0.5%) and Stonebrink media and kept under 37ºC for at least 90 days. The genus of each observed colony was initially confirmed by Ziehl-Neelsen and auramin staining methods. The isolation of Mycobacterium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Cattle; Milk; Public health; Mycobacterium.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962001000600007
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MALDI-TOF mass spectrometry identification and detection of relevant pathogens in beef cattle. Repositório Alice
BACANELLI, G.; MONTOVANI, C.; LOUZAN, A. L.; PASQUATTI, T.; BIER, D.; ROSINHA, G. M. S.; ARAUJO, F. R.; VERBISCK, N. V..
Mycobacterium bovis, Brucella abortus and Salmonella species account for significant economic losses in cattle production worldwide, besides being public health threats. We have established MALDI-TOF (Matrix Assisted Laser Desorption Ionization ? Time-of-Flight) mass spectrometry with modified protein extraction methods and improved the presently available reference spectra libraries, in order to effectively detect those pathogens from bovine samples.
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: Salmonella; Brucella; Mycobacterium; Matrix-assisted laser desorption-ionization mass; Beef cattle.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102578
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Morphometric Analysis of Granulomas Induced by Mycobacterium bovis Suggests an Influence of IFN-Gamma on the Generation and Modulation upon Granulomatous Inflammatory Response in the Different Tissues International Journal of Morphology
Azevedo Duarte,Tonya; Almeida Barbosa Jr.,Aryon de; Arruda,Sergio.
There is evidence in both human and experimental infection caused by Mycobacterium tuberculosis, that immunologic factors influence susceptibility to infection and the progress of the disease. The present study aims to evaluate the role of IFN- g upon inflammatory granulomatous response against M. bovis. To pursue that, C57BL/6 mice lacking the genes for synthesis of IFN- g (IFN- g-/-) and their wild-type counterparts (IFN- g+/+) were intravenously inoculated with M. bovis. The ability of M. bovis to survive and replicate in the liver and lungs was evaluated by counting colony-forming unit (CFU). The histopathological features of granulomatous inflammatory response in the liver and lungs were analyzed during the infection by M. bovis. Granuloma parameters...
Tipo: Journal article Palavras-chave: Morphometry; Mycobacterium; Tuberculosis; IFN-g; Granuloma.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400006
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Mycobacterial spindle cell pseudotumor of the appendix vermiformis in a patient with AIDS BJID
Basílio-de-Oliveira,Carlos Alberto; Eyer-Silva,Walter A.; Valle,Heliomar de Azevedo; Rodrigues,Ana Lúcia; Pimentel,Ana Luisa Pinheiro; Morais-de-Sá,Carlos Alberto.
Mycobacterial pseudotumor (MP) is a rare pathologic presentation of both Mycobacterium tuberculosis and non-tuberculous mycobacterial disease, hitherto reported to occur only in immunosuppressed patients with or without human immunodeficiency virus infection. This lesion shares close pathologic resemblance to certain mesenchymal neoplasms, particularly Kaposi's sarcoma (KS), from which it must be properly differentiated due to distinct prognosis and therapy. We report a case of MP obliterating the lumen of the appendix vermiformis in a 34-year-old patient who died of complications of AIDS at our hospital in Rio de Janeiro. A total of 24 cases of MP (including our patient) have been described in the literature. MP has been found especially in lymph nodes,...
Tipo: Info:eu-repo/semantics/report Palavras-chave: HIV infection; Kaposi's sarcoma; Mycobacterium; Pseudotumor; Spindle cell.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702001000200008
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Mycobacterium genavense infection in two species of captive snakes J. Venom. Anim. Toxins incl. Trop. Dis.
Ullmann,Leila Sabrina; Dias,Ramiro das Neves; Cagnini,Didier Quevedo; Yamatogi,Ricardo Seiti; Oliveira,Jose Paes; Nemer,Viviane; Teixeira,Rodrigo Hidalgo Friciello; Biondo,Alexander Welker; Araújo Jr.,João Pessoa.
Abstract Background: Mycobacterium is an important zoonotic agent with companion, livestock and wildlife animals reportedly playing a role as reservoirs. Although its association with reptiles has been described, the disease cycle remains to be fully established, particularly in snakes. Accordingly, this study aimed to report the occurrence of mycobacteriosis with clinical pneumonia in one exotic python snake (Python molurus) and one native green snake (Philodryas olfersii) from the Sorocaba Zoo, São Paulo state, Brazil. Methods: Diagnosis was based on necropsy, histopathological examination, Ziehl-Neelsen stain and immunohistochemistry. Results: Using a nested PCR followed by DNA sequencing and bioinformatics analysis, the causative Mycobacterium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Captive snakes; Mycobacterium; Public health.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100320
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Occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk in the northwestern region of Paraná, Brazil BJM
Sgarioni,Sônia Aparecida; Hirata,Rosario Dominguez Crespo; Hiroyuki Hirata,Mario; Leite,Clarice Queico Fujimura; Prince,KarinaAndrade de; Leite,Sergio Roberto de Andrade; Vedovello Filho,Dirceu; Siqueira,Vera Lucia Dias; Caleffi-Ferracioli,Katiany Rizzieri; Cardoso,Rosilene Fressatti.
Milk is widely consumed in Brazil and can be the vehicle of agent transmission. In this study, was evaluated the occurrence of Mycobacterium bovis and non-tuberculous mycobacteria (NTM) in raw and pasteurized milk consumed in the northwestern region of Paraná, Brazil. Fifty-two milk samples (20 pasteurized and 32 raw) from dairy farms near the municipality of Maringa, Parana State, Brazil were collected. Milk samples were decontaminated using 5% oxalic acid method and cultured on Lowenstein-Jensen and Stonebrink media at 35 °C and 30 °C, with and without 5-10% CO2. Mycobacteria isolates were identified by morphological features, PCR-Restriction Fragment Length Polymorphism Analysis (PCR-PRA) and Mycolic acids analysis. Thirteen (25%) raw and 2 (4%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Non-tuberculous mycobacteria; Milk; Mycobacterium; PCR-PRA; Mycolic acids analysis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200046
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Ocorrência de tuberculose em rebanhos bubalinos (Bubalus bubalis var. bubalis-Linneus, 1758) no Município de Parintins, Amazonas Arq. Bras. Med. Vet. Zootec.
Mota,P.M.P.C.; Lobato,F.C.F.; Assis,R.A.; Lage,A.P.; Parreiras,P.M.; Leite,R.C..
A comparative tuberculinization test was performed in 1303 animals from seven farms and 266 showed positive to the test. Mycobacterium bovis was isolated from a pool of lymphatic nodes from 14 reagents animals using Stonebrink media and confirmed by biochemical tests.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Búfalo; Tuberculose; Isolamento; Mycobacterium; Bovis.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000400017
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Paratuberculosis: an update BJM
Lilenbaum,W.; Marassi,C.D.; Oelemann,W.M.R..
Paratuberculosis is a chronic enteritis that affects ruminants and is caused by Mycobacterium avium paratuberculosis (Map). The disease is worldwide spread and causes important economic losses. In Brazil, the bacillus was isolated in the south and northeast regions of the country and in Rio de Janeiro, but there are no enough epidemiological studies about its occurrence. Isolation of Map from tissues or fecal samples is 100% specific, but Map shows the most fastidious growth of all mycobacteria. Incubation lasts 8 -12 weeks, with a dependency on exogenous mycobactin J. Diagnostic tests based on specific DNA sequences allow fast and secure identification, and PCR has been used to confirm positive culture results and to identify Map in feces, milk and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paratuberculosis; Bovine; Mycobacterium; Cattle; Map.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400001
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Viability of stressed Mycobacterium tuberculosis and association with multidrug resistance BJM
Martins,Maria Conceição; Giampaglia,Carmen Maria Saraiva; Chimara,Erica; Oliveira,Rosângela Siqueira; Vedovello,Danielle; Sakamoto,Sidnei Miyoshi; Ferrazoli,Lucilaine.
This study investigated biological characteristics of recovered stressed M. tuberculosis isolates that failed to grow in differential culture media for phenotypic identification and in culture media containing anti-tuberculosis drugs for drug-susceptibility testing, despite of having grown in primary culture. It represents an improvement in the diagnosis of MDR tuberculosis and tuberculosis control.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Mycobacterium; Multidrug resistance; Culture growth; Spoligotyping.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200019
Registros recuperados: 16
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