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Registros recuperados: 26
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Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil BABT
Trombete,Felipe Machado; Castro,Izabela Miranda de; Teixeira,Alessandra da Silva; Saldanha,Tatiana; Fraga,Marcelo Elias.
This study aimed to determine the occurrence of AFM1 contamination in the samples of grated parmesan cheese marketed in the Metropolitan Region of Rio de Janeiro -Brazil. Thirty samples representing 10 major brands marketed in the region were analyzed by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD) after purification with immunoaffinity column. The method showed recovery values within the range of 70-90%, with RSD lower than 15% and limits of detection and quantification below the maximum level allowed by the European Commission for the presence of AFM1 in cheeses. The mycotoxin was identified in 18 (60%) of the grated cheese samples tested. The highest value corresponded to 0.69 ± 0.02 µg/kg and the mean for all the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycotoxin; Dairy products; HPLC-FLD.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200016
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Ameliorative potential of vitamin E on the impact of dietary fumonisin B1 on reproductive performance of female rabbits JARTS
Gbore, Francis Ayodeji; Adu, Olufemi Adesanya.
Fumonisin B_1 (FB_1), a contaminant of agricultural products, particularly maize worldwide is known to be consumed by farm animals and has been documented to cause various physiological responses in animals. A 15-week trial on the ameliorative potential of vitamin E on the negative impacts of FB_1 on reproductive performance of rabbits was conducted. Forty-nine female rabbits aged 16 to 18 weeks weighing 1.65 to 2.0 kg body weight were assigned to seven experimental feeding groups: the control group received a diet without FB_1, three groups were fed diets containing different concentrations of FB_1 at 2.5, 5.0 or 7.5 mg kg^(−1), and three further groups had diets containing FB_1 and vitamin E i.e., 2.5 mg FB_1 kg^(−1) + 100 mg vitamin E, 5.0 mg FB_1...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Animal Husbandry rabbit; Fumonisin B1; Mycotoxin; Reproduction; Antioxidant.
Ano: 2017 URL: http://www.jarts.info/index.php/jarts/article/view/2017032852303
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Animal nutrition studies Thai Agricultural
Prateap Rachapaetayakom.
20 tables
Palavras-chave: Chickens; Chicks; Corn; Maize; Opaque-2; Protein diet; Rations; Nutritive value; Varieties; Mycotoxin; Feed contamination; Growth; Animal performance; ข้าวโพด; ยีนโอเพค 2; โปรตีน; สูตรอาหาร; ลูกไก่; ไก่กระทง.
Ano: 1971 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/3767
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Association of the severity of tongue lesions in broiler chickens with the quality of corn in feed PAB
Vaccari,Isabel Cristina Mores; Duarte,Vinicius; Rodrigues,Denise Russi; Santos,Fabiana Ramos dos; Minafra,Cibele Silva.
Abstract: The objective of this work was to determine the correlation of the physical attributes of corn and the mycotoxins present in its grains with the severity of tongue lesions in broiler breeder roosters. The occurrence of the aflatoxin, vomitoxin, zearalenone, T-2 toxin, and fumonisin mycotoxins was detected in the corn grains used in the feed formulation for breeder chickens. The percentages of moisture and broken grains were positively correlated (Pearson’s correlation) with the presence of aflatoxin. Milder lesions (grade 1) are positively correlated with aflatoxin and negatively correlated with fumonisin, and the most severe lesions (grade 3) are positively correlated with vomitoxin, fumonisin, and T-2 toxin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin; Aviculture; Fumonisin; Mycotoxin; T-2 toxin; Vomitoxin..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017001000942
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Deoxynivalenol (DON) degradation and peroxidase enzyme activity in submerged fermentation Ciênc. Tecnol. Aliment.
Garda-Buffon,Jaqueline; Kupski,Larine; Badiale-Furlong,Eliana.
This work aims to evaluate deoxynivalenol degradation by Aspergillus oryzae and Rhizopus oryzae in a submerged fermentation system and to correlate it to the activity of oxydo-reductase enzymes. The submerged medium consisted of sterile distilled water contaminated with 50 μg of DON and 4 × 10(6) spore.mL-1 inoculum of Aspergillus oryzae and Rhizopus oryzae species, respectively in each experiment. Sampling was performed every 24 hours for monitoring the peroxidase specific activity, and every 48 hours for determining mycotoxin levels. Results showed that the fungi species were able to decrease DON levels as the peroxidase activity increased. The 48 hours fermentation interval presented the highest peroxidase specific activity (ΔABS/minute.μg.protein-1),...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycotoxin; Peroxidase enzyme; Degradation; Fermentation; Fungal species.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000100030
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Development and validation of a method for detection and quantification of ochratoxin A in green coffee using liquid chromatography coupled to mass spectrometry Ciênc. Tecnol. Aliment.
Bandeira,Raquel Duarte da Costa Cunha; Uekane,Thaís Matsue; Cunha,Carolina Passos da; Cunha,Valnei Smarçaro da; Rodrigues,Janaína Marques; Godoy,Ronoel Luiz de Oliveira; la Cruz,Marcus Henrique Campino de.
A method using Liquid Chromatography Tanden Mass Spectrometry (LC-MS/MS) with matrix-matched calibration curve was developed and validated for determining ochratoxin A (OTA) in green coffee. Linearity was found between 3.0 and 23.0 ng.g-1. Mean recoveries ranged between 90.45% and 108.81%; the relative standard deviation under repeatability and intermediate precision conditions ranged from 5.39% to 9.94% and from 2.20% to 14.34%, respectively. The limits of detection and quantification were 1.2 ng.g-1 and 3.0 ng.g-¹, respectively. The method developed was suitable and contributed to the field of mycotoxin analysis, and it will be used for future production of the Certified Reference Material (CRM) for OTA in coffee.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee; Mycotoxin; Validation; HPLC; MS/MS.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000400021
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Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain PAB
Silva,André Ribeiro da; Zanin,Lívia Montanheiro Médici; Ishikawa,Angélica Tieme; Yamashita,Cassia Reika Takabayashi; Fracalossi,Felipe Pedote; Amorim,Thaís Marques; Itano,Eiko Nakagawa; Kawamaura,Osamu; Hirooka,Elisa Yoko.
Abstract: The objective of this work was to standardize and validate an indirect competitive enzyme-linked immunosorbent assay (ic-Elisa), as a low-cost tool, to monitor the presence of aflatoxin in common and blanched peanuts (Arachis hypogaea) in the production chain. The presence of aflatoxin B1, moisture content, and water activity were analyzed in 60 samples of the peanut cultivar Runner IAC 886, from the 2014/2015 and 2015/2016 harvests of the region of Alta Paulista, in the state of São Paulo, Brazil. The validation showed an adequate linearity (R2 = 0.999), and limits of detection and quantification of 1.13 and 3.59 μg kg-1, respectively. Recovery rates of 104, 102, and 107% at the concentrations of 4, 10, and 20 μg kg-1 aflatoxin B1, respectively,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus; Aflatoxin B1; Blanched peanuts; Contamination; Mycotoxin.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300361
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Distribution of Fusarium mycotoxins in wheat milling process. Repositório Alice
TIBOLA, C. S.; FERNANDES, J. M. C.; GUARIENTI, E. M.; NICOLAU, M..
2016
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Wheat; Mycotoxin; Deoxynivalenol; Ergosterol; Milling process; Trigo; Micotoxina.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1048814
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DNA damage by ochratoxin A in rat kidney assessed by the alkaline comet assay BJMBR
Zeljezic,D.; Domijan,A.-M.; Peraica,M..
There are few studies of ochratoxin A (OTA) genotoxicity in experimental animals and the results obtained with cell cultures are inconsistent, although the carcinogenic potential of OTA for the kidney of experimental animals has been well established. We studied the genotoxic potential of OTA in the kidney of adult female Wistar rats (5 in each group) treated intraperitoneally with OTA (0.5 mg kg body weight-1 day-1 for 7, 14, and 21 days) measuring DNA mobility on agarose gel stained with ethidium-bromide using standard alkaline single-cell gel electrophoresis (comet assay). Negative control animals were treated with solvent (Tris buffer, 1.0 mg/kg) and positive control animals were treated with methyl methanesulfonate (40 mg/kg) according to the same...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Comet assay; DNA adducts; Experimental animals; Mycotoxin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001200006
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Effect of fungicide on Fusarium verticillioidesmycelial morphology and fumonisin B1 production BJM
Miguel,Tatiana de Á.; Bordini,Jaqueline G.; Saito,Gervásio H.; Andrade,Célia G.T. de J.; Ono,Mario A.; Hirooka,Elisa Y.; Vizoni,Édio; Ono,Elisabete Y.S..
The effect of fludioxonil + metalaxyl-M on the mycelial morphology, sporulation and fumonisin B1 production by Fusarium verticillioides 103 F was evaluated. Scanning electron microscopy analysis showed that the fungicide caused inhibition of hyphal growth and defects on hyphae morphology such as cell wall disruption, withered hyphae, and excessive septation. In addition, extracellular material around the hyphae was rarely observed in the presence of fludioxonil + metalaxyl-M. While promoting the reduction of mycelial growth, the fungicide increased sporulation of F. verticillioides compared to the control, and the highest production occurred on the 14th day in the treatments and on the 10th day in the control cultures. Fumonisin B1production in the culture...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxigenic fungi; Mycotoxin; Scanning electron microscopy; Electron micrographs; Extracellular material.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100293
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Effects of baking process on mycotoxin content in whole and white breads. Repositório Alice
TIBOLA, C. S.; MIRANDA, M. Z. de; PAIVA F. F.; FERNANDES, J. M. C.; GUARIENTI, E. M.; NICOLAU, M..
2016
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Mycotoxin; Artificial inoculation; Deoxynivalenol; Breadmaking; Micotoxina; Inoculação artificial; Panificação.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1048816
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Effects of fed mycotoxin contaminated diets supplemented with spray-dried porcine plasma on cholinergic response and behavior in piglets Anais da ABC (AABC)
MÜLLER,LUCIELI K.F.; SILVA,ALEKSANDRO S. DA; BOTTARI,NATHIELI B.; SANTURIO,JANIO M.; MORSCH,VERA M.; PIVA,MANOELA M.; MENDES,RICARDO E.; GLORIA,EDUARDO M.; PAIANO,DIOVANI.
Abstract: The aim of this study was to evaluate the effect of spray-dried porcine plasma (SDPP) supplementation on cholinesterase enzymes and its relationship with animal behavior of weaning piglets exposed to mycotoxin contaminated diets. To achieve these objectives, two experimental design approaches were used. Male piglets (7.15±0.61kg) were allocated in four groups: CTL group received a regular diet; SDPP group received a regular diet and 6% SDPP; MYC group received a diet containing desired contamination of 210 µg/kg aflatoxins and 6.690 µg/kg fumonisins; group MYC+SDPP received 253 µg/kg aflatoxins, 6930 µg/kg fumonisins and 6% SDPP. The animals treated with mycotoxin co-contaminated diets showed an increase in AChE and BChE activities in peripheral...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycotoxin; Acetylcholinesterase; Brain; Spray-dried porcine plasma; Animal health.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300807
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Effects of fusaric acid on Zea mays L. seedlings Phyton
Diniz,SPSS; Oliveira,RC.
The effects of fusaric acid, a mycotoxin produced by Fusarium sp, were investigated in relation to its action on corn seedlings. The mycotoxin inhibited the development of corn seedlings at 0.2 mM, causing root length reduction. Anatomically, alterations were only visible from 0.5 mM fusaric acid, directly influencing the cell differentiation process. Precocious differentiation reduces the elongation region. This explains (1) that root shortening is morphologically visible, and (2) the differentiation of a great number of lateral roots nearby the apex, which can be seen in longitudinal cuts.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusaric acid; Mycotoxin; Zea mays seedlings.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000200012
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Effects of ochratoxin a on broiler leukocytes Rev. Bras. Ciênc. Avic.
Moura,MA; Machado,CH; Porfírio,LC; Freire,RB.
This study evaluated alterations in the qualitative cellular profile of leukocytes caused by the administration of low doses of ochratoxin-A (OTA) in poultry. Sixty chicks were separated in three experimental groups: control, PBS-treated and OTA-treated. Blood smears from all birds were analyzed three and six hours post-treatment. Differential leukocyte counting demonstrated that OTA reduced the percentage of lymphocytes and eosinophils and significantly increased the number of heterophils and monocytes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broilers; Hematology; Mycotoxin; Ochratoxin A.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000300010
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Função hepática e renal de frangos de corte alimentados com dietas com aflatoxinas e clinoptilolita natural Repositório Alice
MACIEL, R.M.; LOPES, S.T. dos A.; SANTURIO, J.M.; MARTINS, D.B.; ROSA, A.P.; EMANUELLI, M.P..
O objetivo deste trabalho foi avaliar os minerais séricos e as funções hepática e renal de frangos de corte, alimentados com dietas com aflatoxinas e dietas com aflatoxinas e argila clinoptilolita natural. Foram utilizados 528 frangos de corte machos, da linhagem Ross, distribuídos em seis tratamentos com quatro repetições cada: T1 - testemunha: ração sem aflatoxina ou clinoptilolita; T2 - ração com 5 ppm de aflatoxinas; T3 - ração com 0,25% de clinoptilolita; T4 - ração com 5 ppm de aflatoxinas e 0,25% de clinoptilolita; T5 - ração com 0,5% de clinoptilolita; e T6 - ração com 5 ppm de aflatoxinas e 0,5% de clinoptilolita. As aves foram submetidas aos tratamentos do 1o ao 42o dia de idade. Foram abatidos 72 animais, e foram analisadas as concentrações...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Micotoxina; Concentração de minerais; Adsorvente; Nutrição animal; Mycotoxin; Mineral concentration; Adsorbent; Zeolite; Animal nutrition.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/handle/doc/126302
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Identification of a splicing coactivator gene that affects the production of ochratoxin a in Aspergillus carbonarius BABT
Lunardi,Lígia Uno; Guembarovski,Roberta Losi; Hanai,Luiz Ricardo; Cristiano,Valderi; Vieira,Maria Lucia Carneiro; Sartori,Daniele; Fungaro,Maria Helena Pelegrinelli.
Ochratoxin A is a mycotoxin produced by some fungi species. Among them, Aspergillus carbonarius is considered a powerful producer. Genes involved in the ochratoxin A biosynthesis pathway have been identified in some producer species. However, there are few studies that purpose to identify these genes in A. carbonarius. The use of insertion mutants to identify genes associated with certain properties has been increased in the literature. In this work, the region of T-DNA integration was investigated in one A. carbonarius ochratoxin-defective mutant previously obtained by Agrobacterium tumefaciens-mediated transformation, in order to find an association between interrupted gene and the biosynthesis of ochratoxin A. The integration occurred in a gene that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ochratoxigenic fungi; Mycotoxin; Real time PCR; Splicing coactivator protein.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700018
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Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC) Chilean J. Agric. Res.
Moyano A,Stella; Lanuza,Francisco; Torres B,Alfredo; Cisternas A,Ernesto; Fuentes V,Marcela.
Lolitrem-B is a neurotoxic substance that causes the ryegrass staggers syndrome in cattle, sheep, and horses consuming ryegrass (Lolium perenne L.) infected with endophytic fungus Neotyphodium lolii (Latch, Christensen, and Samuels) Glenn, Bacon, and Hanlin. Due to the increasing use of ryegrass pasture in southern Chile, it is necessary to know the content of lolitrem-B to optimize grazing management and reduce the effect on animal health. The objective of this study was to implement a method to determine lolitrem-B in ryegrass. Ryegrass samples were used without the endophyte, freeze-dried, and kept in darkness at -18 ºC to establish the method. Then, a pure lolitrem-B standard was added and the toxin was extracted with a mixture of chloroform-methanol...
Tipo: Journal article Palavras-chave: Mycotoxin; Neurotoxin; Endophyte fungi.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300019
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Influence of drying methods on cocoa (Theobroma cacao L.): antioxidant activity and presence of ochratoxin A Ciênc. Tecnol. Aliment.
DEUS,Valterney Lima; CERQUEIRA E SILVA,Mariana Barros de; MACIEL,Leonardo Fonseca; MIRANDA,Lucas Caldeirão Rodrigues; HIROOKA,Elisa Yoko; SOARES,Sérgio Eduardo; FERREIRA,Ederlan de Souza; BISPO,Eliete da Silva.
Abstract This work aimed to evaluate the performance of different drying methods in the development of phenolic compounds, methylxanthines, and antioxidant activity in cocoa bean and to evaluate the presence of ochratoxin A (OTA). Antioxidant activity was determined using DPPH, FRAP, ORAC and β-carotene-linoleic acid assays. Phenolic compounds and methylxanthines were determined using high-performance liquid chromatography with an ultraviolet detector (HPLC-UV). OTA was determined by ultra-performance liquid chromatography with fluorescence detection (UPLC-FLR). It was found that during cocoa drying, there was a reduction in antioxidant activity with reduced phenolic compound content (0.04 mg.g-1 of catechin before drying to 0.02 mg.g-1 after drying) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catechin; Epicatechin; Caffeine; Theobromine; Mycotoxin.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000500278
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MEIS3 is repressed in A549 lung epithelial cells by deoxynivalenol and the repression contributes to the deleterious effect OAK
Toyotome, Takahito; Takahashi, Hiroki; Kamei, Katsuhiko.
Deoxynivalenol (DON) is an important Fusarium toxin of concern for food safety. The inhalation of powder contaminated with DON is possible and may cause lung toxicity. In this study, we analyzed the gene expression profile of A549 cells treated for 24 hr with 0.2 µg/mL DON by microarray analysis. In total, 16 genes and 5 noncoding RNAs were significantly affected by DON treatment. The repression of B3GALT4, MEIS3, AK7, SEMA3A, KCNMB4, and SCARA5 was confirmed by quantitative PCR. We investigated the DON toxicity on A549 cells that exogenously expressed these 6 genes. The result indicated that A549 cells that transiently expressed MEIS3 were tolerant to the deleterious effects of DON. Our data show that DON affected the expression of genes with various...
Palavras-chave: Fusarium toxin; Mycotoxin; Deoxynivalenol; Trichothecene; Lung epithelial cell.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4301
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Molecular identification of Aspergillus spp. isolated from coffee beans Scientia Agricola
Magnani,Marciane; Fernandes,Thiago; Prete,Cássio Egidio Cavenaghi; Homechim,Martin; Ono,Elisabete Yurie Sataque; Vilas-Boas,Laurival Antonio; Sartori,Daniele; Furlaneto,Márcia Cristina; Fungaro,Maria Helena Pelegrinelli.
Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ITS; Ribosomal DNA; Ochratoxin; Mycotoxin.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100009
Registros recuperados: 26
Primeira ... 12 ... Última
 

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