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| Carmo,Fausto Moreira da Silva; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Pires,Aldrin Vieira; Guimarães,Marta Fonseca Martins; Silva,Marcos Vinícius Gualberto Barbosa da; Schierholt,Alex Sandro; Silva,Kleibe de Moraes e; Gomide,Lúcio Alberto de Miranda. |
| We studied the phenotypic effects of polymorphisms at the MYF5 gene in a divergent F2 swine population and found that one polymorphism was due to an insertion and another to a deletion. The genotypes of 359 F2 animals were obtained and the Normal/Normal (NN) and Normal/Insertion (NI) genotypes analyzed to determine associations with phenotypic data for performance, carcass and meat quality traits. Significant differences were observed (p < 0.05) between NN and NI animals for drip (NN = 3.14 ± 1.56; NI = 3.69 ± 2.78%), cooking (NN = 32.26 ± 2.41; NI = 33.21 ± 2.31%) and total loss (NN = 34.16 ± 2.63 and NI = 34.97 ± 2.08%). The Deletion marker was not statistically tested. The results indicate that the allelic variant Insertion is associated with a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myogenesis; DNA sequencing; Pig production; PCR. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300004 |
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| Costa,M.L.; Escaleira,R.; Manasfi,M.; Souza,L.F. de; Mermelstein,C.S.. |
| The current myogenesis and myofibrillogenesis model has been based mostly on in vitro cell culture studies, and, to a lesser extent, on in situ studies in avian and mammalian embryos. While the more isolated artificial conditions of cells in culture permitted careful structural analysis, the actual in situ cellular structures have not been described in detail because the embryos are more difficult to section and manipulate. To overcome these difficulties, we used the optically clear and easy to handle embryos of the zebrafish Danio rerio. We monitored the expression of cytoskeletal and cell-adhesion proteins (actin, myosin, desmin, alpha-actinin, troponin, titin, vimentin and vinculin) using immunofluorescence microscopy and video-enhanced,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytoskeleton; Cell adhesion; Zebrafish; Myogenesis. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800019 |
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| Costa,M.L.; Escaleira,R.; Cataldo,A.; Oliveira,F.; Mermelstein,C.S.. |
| Desmin is the intermediate filament (IF) protein occurring exclusively in muscle and endothelial cells. There are other IF proteins in muscle such as nestin, peripherin, and vimentin, besides the ubiquitous lamins, but they are not unique to muscle. Desmin was purified in 1977, the desmin gene was characterized in 1989, and knock-out animals were generated in 1996. Several isoforms have been described. Desmin IFs are present throughout smooth, cardiac and skeletal muscle cells, but can be more concentrated in some particular structures, such as dense bodies, around the nuclei, around the Z-line or in costameres. Desmin is up-regulated in muscle-derived cellular adaptations, including conductive fibers in the heart, electric organs, some myopathies, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Desmin; Intermediate filaments; Muscle; Myogenesis; Myopathy. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200007 |
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| Fukushima,M.G.; Furlan,I.; Chiavegatti,T.; Kiyomoto,B.H.; Godinho,R.O.. |
| The present study analyzes the ectopic development of the rat skeletal muscle originated from transplanted satellite cells. Satellite cells (10(6) cells) obtained from hindlimb muscles of newborn female 2BAW Wistar rats were injected subcutaneously into the dorsal area of adult male rats. After 3, 7, and 14 days, the transplanted tissues (N = 4-5) were processed for histochemical analysis of peripheral nerves, inactive X-chromosome and acetylcholinesterase. Nicotinic acetylcholine receptors (nAChRs) were also labeled with tetramethylrhodamine-labeled alpha-bungarotoxin. The development of ectopic muscles was successful in 86% of the implantation sites. By day 3, the transplanted cells were organized as multinucleated fibers containing multiple clusters of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Skeletal muscle; Satellite cells; Stem cells; Transplantation; Myogenesis; 2BAW rats. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000300007 |
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| Reis,Evelyze Pinheiro dos; Paixão,Débora Martins; Brustolini,Otávio José Bernardes; Silva,Fabyano Fonseca e; Silva,Walmir; Araújo,Flávio Marcos Gomes de; Salim,Anna Christina de Matos; Oliveira,Guilherme; Guimarães,Simone Eliza Facioni. |
| Abstract This study used qRT-PCR to examine variation in the expression of 13 myogenes during muscle development in four prenatal periods (21, 40, 70 and 90 days post-insemination) in commercial (the three-way Duroc, Landrace and Large-White cross) and local Piau pig breeds that differ in muscle mass. There was no variation in the expression of the CHD8, EID2B, HIF1AN, IKBKB, RSPO3, SOX7 and SUFU genes at the various prenatal ages or between breeds. The MAP2K1 and RBM24 genes showed similar expression between commercial and Piau pigs but greater expression (p < 0.05) in at least one prenatal period. Pair-wise comparisons of prenatal periods in each breed showed that only the CSRP3, LEF1, MRAS and MYOG genes had higher expression (p < 0.05) in at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Commercial line; Gene expression; Myogenesis; Pig; Piau breed. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400589 |
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