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Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 5
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A large-scale analysis of resistance gene analogs (RGAs) encoding NBS domains in the genus Elaeis. Repositório Alice
SANTOS, M. de L.; SANTOS, M. de L.; COTTA, M. G.; FONSECA, F. C. A.; TOGAWA, R. C.; COSTA, M. M. do C.; ALVES, A. A.; MILLER, R. N. G.; SOUZA JUNIOR, M. T..
bitstream/item/173848/1/CBFITO-ePoster637.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: NBS-LRR; Elaeis guineenses; Elaeis oleífera; Biotic stress.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1088997
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Cloning and analysis of a NBS-LRR disease resistance gene candidate PnAG1 from peanut (Arachis hypogaea L.) Electron. J. Biotechnol.
Shan,Shi-hua; Zhang,Ting-ting; Li,Chun-juan; Yang,Chen; Yan,Cai-xia; Wan,Shu-bo.
Background: Based on the conserved sequences of a known NBS resistance gene, a pair of degenerate primers was designed to amplify the NBS-LRR resistance gene from peanut using PCR and RACE methods. Results: Analyzing the amino acid sequence by BLAST on NCBI, which was deduced from the 1088bp-long gene named PnAG1-2, showed that it had a certain homology with some resistance proteins, among which Arachis cardenasii resistance protein gene had the highest homology (66%). Relative quantification PCR analysis indicated that PnAG1-2 gene expresses more in J11 (an A. flavus-resistant variety) than in JH1012 (an A. flavus-susceptible variety) when the harvest time was coming. Conclusions: In this study, the NBS-LRR resistance sequence was successfully cloned from...
Tipo: Journal article Palavras-chave: Bioinformatics; NBS-LRR; Peanut; Real-time fluorescence quantitative PCR.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600006
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Cloning and characterization of resistance gene analogs from under-exploited plant species Electron. J. Biotechnol.
Thirumalaiandi,Ramasubramanian; Selvaraj,Michael Gomez; Rajasekaran,Raghu; Subbarayalu,Mohankumar.
Genomic DNA sequences sharing homology with NBS region of resistance gene analogs were isolated and characterized from Pongamia glabra, Adenanthera pavonina, Clitoria ternatea and Solanum trilobatum using PCR based approach with primers designed from conserved regions of NBS domain. The presence of consensus motifs viz., kinase 1a, kinase 2, kinase 3a and hydrophobic domain provided evidence that the cloned sequences may belong to the NBS-LRR gene family. Conservation of tryptophan as the last residue of kinase-2 motif further confirms their position in non-TIR NBS-LRR family of resistance genes. The Resistance Gene Analogs (RGAs) cloned from P. glabra, A. pavonina, C. ternatea and S. trilobatum clustered together with well- characterized non-TIR-NBS-LRR...
Tipo: Journal article Palavras-chave: Adenanthera pavonina; Clitoria ternatea; NBS-LRR; Pongamia glabra; Resistance gene analogs; Solanum trilobatum.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400004
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Genome-wide identification of NBS resistance genes in Populus trichocarpa Inra
Kohler, A.; Rinaldi, C.; Duplessis, S.; Baucher, M.; Geelen, D.; Duchaussoy, F.; Meyers, BC; Boerjan, W.; Martin, F..
As the largest class of disease resistance R genes, the genes encoding nucleotide binding site and leucine-rich repeat proteins (“NBS-LRR genes”) play a critical role in defending plants from a multitude of pathogens and pests. The diversity of NBS-LRR genes was examined in the Populus trichocarpa draft genome sequence. The NBS class of genes in this perennial tree is large and diverse, comprised of ∼400 genes, at least twice the complement of Arabidopsis. The NBS family can be divided into multiple subfamilies with distinct domain organizations. It includes 119 Coiled-Coil-NBS-LRR genes, 64 TIR-NBS-LRR genes, 34 BED-finger-NBS-LRR, and both truncated and unusual NBS- and NBS-LRR-containing genes. The transcripts of only 34 NBS-LRR genes were detected in...
Tipo: Journal Article Palavras-chave: Populus; NBS-LRR; Resistance.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010b08a4238&uri=/notices/prodinra1/2010/10/
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Molecular diversity at the Vat/Pm-W resistance locus in melon National Institute of Agronomic Research
Dogimont, C.; Chovelon, V.; Tual, S.; Boissot, N.; Rittener-Rüff, V.; Giovinazzo, N.; Bendahmane, A..
The resistance Vat gene of melon was isolated by map-based cloning. It encodes a protein with a coiled coil domain (CC), a nucleotide binding site (NBS) and leucine–rich repeats (LRR). Whereas most resistance genes confer resistance to a single pathogen, two alleles with different resistance specificities were identified at the locus. The Vat allele confers resistance to plant colonization by the aphid Aphis gossypii and to transmission of unrelated viruses by this vector specifically; the Pm-W allele confers resistance to the powdery mildew Podosphaera xanthii. Here we identified Vat-analogs in several melon genotypes using Long Range PCR, cloning and sequencing of full-length fragments. Several sources of variability were identified: (i) a different...
Tipo: Conference Paper Palavras-chave: Aphid resistance; Aphis gossypii; Powdery mildew resistance; Podosphaera xanthii; NBS-LRR; Disease resistance; Cucumis melo; Virus vector; PCR; Cloning; Sequencing; Molecular diversity.
Ano: 2008 URL: http://hdl.handle.net/2174/211
Registros recuperados: 5
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