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Registros recuperados: 10
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Imprime registro no formato completo
CDH1 mutations in gastric cancer patients from northern Brazil identified by Next- Generation Sequencing (NGS) Genet. Mol. Biol.
El-Husny,Antonette; Raiol-Moraes,Milene; Amador,Marcos; Ribeiro-dos-Santos,André M.; Montagnini,André; Barbosa,Silvanira; Silva,Artur; Assumpção,Paulo; Ishak,Geraldo; Santos,Sidney; Pinto,Pablo; Cruz,Aline; Ribeiro-dos-Santos,Ândrea.
Abstract Gastric cancer is considered to be the fifth highest incident tumor worldwide and the third leading cause of cancer deaths. Developing regions report a higher number of sporadic cases, but there are only a few local studies related to hereditary cases of gastric cancer in Brazil to confirm this fact. CDH1 germline mutations have been described both in familial and sporadic cases, but there is only one recent molecular description of individuals from Brazil. In this study we performed Next Generation Sequencing (NGS) to assess CDH1 germline mutations in individuals who match the clinical criteria for Hereditary Diffuse Gastric Cancer (HDGC), or who exhibit very early diagnosis of gastric cancer. Among five probands we detected CDH1 germline...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CDH1; Germline mutations; HDGC; Gastric Cancer; NGS.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200189
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Desenvolvimento de marcadores microssatélites por meio do sequenciamento parcial do genoma de maracujá-azedo (Passiflora Edulis Sims) e transferibilidade para 78 espécies do gênero passiflora. Repositório Alice
ARAYA, S.; FALEIRO, F. G.; JUNQUEIRA, N. T. V.; MARTINS, A. M.; COSTA, A. M.; FERREIRA, M. E..
bitstream/item/171191/1/Anais-Simposio-p19.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: NGS; Genômica; Passiflora; Maracujá; Recurso Genético; Melhoramento Genético Vegetal.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085577
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Effect of vacuum and modified atmosphere packaging on the microbiological, chemical and sensory properties of tropical red drum ( Sciaenops ocellatus ) fillets stored at 4 °C ArchiMer
Silbande, Adele; Adenet, Sandra; Chopin, Christine; Cornet, Josiane; Smith-ravin, Juliette; Rochefort, Katia; Leroi, Francoise.
Aims The effect of vacuum (VP – 4 °C) and CO2/N2–atmosphere (MAP – 4 °C) packaging on the quality of red drum fillets compared with whole gutted iced fish was investigated. Methods and results A metagenomic approach, bacterial enumeration and isolation, biochemical and sensory analyses were carried out. The organoleptic rejection of whole fish was observed at day 15 whereas VP and MAP fillets appeared unacceptable only after 29 days. At these dates, total mesophilic counts reached 107–108 CFU g− 1. According to Illumina MiSeq sequencing, Arthrobacter, Chryseobacterium, Brevibacterium, Staphylococcus and Kocuria were the main genera of the fresh red drum fillets. At the sensory rejection time, lactic acid bacteria (LAB), particularly Carnobacterium sp.,...
Tipo: Text Palavras-chave: Seafood; Biogenic amine; VP; MAP; NGS; 16S rRNA gene.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00407/51858/52468.pdf
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Expressão diferencial de genes em Pleurotus pulmonarius associados a degradação enzimática e destoxificação da torta de pinhão-manso. Repositório Alice
GOMES, T. G.; ALVES, G. S. C.; HADI, S. I. I. A.; COSTA, M. M. do C.; MENDONCA, S.; MILLER, R. N. G.; SIQUEIRA, F. G. de.
bitstream/item/184260/1/EnPI-2018-8.pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Transcritoma; NGS; Pré-tratamento biológico; Macrofungos; Jatropha Curcas.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1097269
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GigaTON: an extensive publicly searchable database providing a new reference transcriptome in the pacific oyster Crassostrea gigas ArchiMer
Riviere, Guillaume; Klopp, Christophe; Ibouniyamine, Nabihoudine; Huvet, Arnaud; Boudry, Pierre; Favrel, Pascal.
Background: The Pacific oyster, Crassostrea gigas, is one of the most important aquaculture shellfish resources worldwide. Important efforts have been undertaken towards a better knowledge of its genome and transcriptome, which makes now C. gigas becoming a model organism among lophotrochozoans, the under-described sister clade of ecdysozoans within protostomes. These massive sequencing efforts offer the opportunity to assemble gene expression data and make such resource accessible and exploitable for the scientific community. Therefore, we undertook this assembly into an up-to-date publicly available transcriptome database: the GigaTON (Gigas TranscriptOme pipeliNe) database. Description: We assembled 2204 million sequences obtained from 114 publicly...
Tipo: Text Palavras-chave: Oyster; Transcriptome; Development; Stress; Tissues; NGS.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00302/41321/40520.pdf
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High-throughput sequencing applied for the identification of viruses infecting grapevines in Brazil and genetic variability analysis. Repositório Alice
FAJARDO, T. V. M.; SILVA, F. N.; EIRAS, M.; NICKEL, O..
The application of high-throughput sequencing technologies (HTS) enables the recovery of many nucleotide sequence fragments from diseased plants and may help in pathogen identification. This study was designed to identify viruses infecting 15 grapevine (Vitis spp.) samples collected from experimental fields and vine collections and assess the genetic variability of the identified viruses. The virus-enriched dsRNAs were extracted from bark scrapings and sequenced using an Illumina platform. The paired-end reads were analyzed, assembled contigs were generated and identified as related to viruses. Contigs of 14 viruses have been identified, some of them covering large extensions of viral genomes or resulting in assembly of near-complete or complete genomes....
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Diagnosis; HTS; Next-generation sequencing; NGS; Vitis; Variability.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1077833
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Impact of NGS in the medical sciences: genetic syndromes with an increased risk of developing cancer as an example of the use of new technologies Genet. Mol. Biol.
Lapunzina,Pablo; López,Rocío Ortiz; Rodríguez-Laguna,Lara; García-Miguel,Purificación; Martínez,Augusto Rojas; Martínez-Glez,Víctor.
The increased speed and decreasing cost of sequencing, along with an understanding of the clinical relevance of emerging information for patient management, has led to an explosion of potential applications in healthcare. Currently, SNP arrays and Next-Generation Sequencing (NGS) technologies are relatively new techniques used to scan genomes for gains and losses, losses of heterozygosity (LOH), SNPs, and indel variants as well as to perform complete sequencing of a panel of candidate genes, the entire exome (whole exome sequencing) or even the whole genome. As a result, these new high-throughput technologies have facilitated progress in the understanding and diagnosis of genetic syndromes and cancers, two disorders traditionally considered to be separate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: NGS; New technologies; Developmental syndrome; Cancer predisposition.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200010
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Imprime registro no formato completo
Next Generation Sequencing (NGS) for discovery of droughtresponsive genes in wild Arachis. Repositório Alice
BRASILEIRO, A. C. M.; MORGANTE, C. V.; SILVA, A. K.; MARTINS, A. C. Q.; PAPPAS JUNIOR, G. J.; SILVA JUNIOR, O. B. da; BERTIOLI, D. J.; BERTIOLI, S. C. de M. L.; GUIMARAES, P. M..
2011
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: NGS; Amemdoim; Diversidade genética; Marcador molecular; Seca; Amemdoim.
Ano: 2011 URL: http://www.alice.cnptia.embrapa.br/handle/doc/886665
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Imprime registro no formato completo
Quality assessment of ice-stored tropical yellowfin tuna (Thunnus albacares) and influence of vacuum and modified atmosphere packaging ArchiMer
Dauchy Silbande, Adele; Adenet, Sandra; Smith-ravin, Juliette; Joffraud, Jean-jacques; Rochefort, Katia; Leroi, Francoise.
Metagenomic, microbial, chemical and sensory analyses of Thunnus albacares from Martinique and stored in ice (AIR – 0 °C), vacuum (VP – 4/8 °C) and modified atmosphere packaging (MAP – 4/8 °C) (70% CO2 – 30% O2) were carried out. The organoleptic rejection of AIR tuna was observed at day 13 when total bacterial counts equaled 106–107 CFU g−1. No extension of shelf-life was provided by VP and MAP. According to 16S rRNA gene sequence analyzed by Illumina MiSeq and PCR-TTGE, Rhodanobacter terrae was the main species of the freshly caught tuna. At the sensory rejection time, Brochothrix thermosphacta and Pseudomonas dominated the AIR products while B. thermosphacta alone or a mix of B. thermosphacta, Enterobacteriaceae and lactic acid bacteria (LAB) dominated...
Tipo: Text Palavras-chave: Seafood; Fish; Microbiology; Sensory; NGS; 16S rRNA gene.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00344/45504/45056.pdf
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Sobreexpresión de genes en la interacción de Spongospora subterranea (Wallr.) Lagerh. y dos cultivares de Solanum phureja Juz. et. Buk Rev. Protección Veg.
Rodríguez Fuerte,Verónica; Marín Montoya,Mauricio; Morales Osorio,Juan Gonzalo; Cotes Torres,José Miguel; Gutiérrez Sánchez,Pablo Andrés.
Con el fin de contribuir a un mejor conocimiento de los mecanismos que rigen la interacción entre Solanum phureja Juz. et. Buk. y Spongospora subterranea (Wallr.) Lagerh, agente causal de la sarna polvosa de la papa, se realizó en esta investigación el análisis del transcriptoma de dos cultivares: Criolla Colombia (susceptible) y Criolla Latina (tolerante), mediante pirosecuenciación 454. Los resultados indicaron diferencias entre los genes sobreexpresados en cada cultivar ante la infección con el patógeno. En el cultivar susceptible, la respuesta ocurre a partir de la activación transcripcional de genes asociados a la integridad de la pared celular, transducción de señales y genes involucrados en la respuesta a diferentes tipos de estrés. Los genes que...
Tipo: Journal article Palavras-chave: NGS; PCR en tiempo real; Sarna polvosa; Transcriptoma.
Ano: 2014 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000100003
Registros recuperados: 10
Primeira ... 1 ... Última
 

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