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A 31P NMR study of phosphate levels in roots of ectomycorrhizal and nonmycorrhizal Castanea sativa IPB - Escola Superior Agrária
Martins, Anabela; Santos, M.M.; Santos, Helena; Pais, M.S..
31 P-Nuclear Magnetic Resonance (NMR) was used to assess phosphate distribution in ectomycorrhizal and nonmycorrhizal roots of Castanea sativa Mill. as well as in the mycorrhizal fungus Pisolithus tinctorius in order to gain insight into phosphate trafficking in these systems. The fungus P. tinctorius accumulated high levels of polyphosphates during the rapid phase of growth. Mycorrhizal and nonmycorrhizal roots accumulate orthophosphate. Only mycorrhizal roots presented polyphosphates. The content in polyphosphates increased along the 3 months of mycorrhiza formation. In mycorrhizal roots of plants cultured under axenic conditions, the orthophosphate pool decreased along the culture time. In nonmycorrhizal roots the decrease in the orthophosphate content...
Tipo: Article Palavras-chave: Castanea sativa; Pisolithus tinctorius; Ectomycorrhiza; Phosphate; NMR.
Ano: 1999 URL: http://hdl.handle.net/10198/6420
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A di-O-dihydrogeranylgeranyl glycerol from Thermococus S 557, a novel ether lipid, and likely intermediate in the biosynthesis of diethers in Archaea ArchiMer
Gonthier, Isabelle; Rager, Marie-noëlle; Metzger, Pierre; Guezennec, Jean; Largeau, Claude.
The lipids of a deep-sea hydrothermal vent arch ae on, Thermococcus S 557, were isolated, purified and structurally determined. Based on acid methanolysis and spectroscopic studies, the polar lipids were shown to comprise diphytanyl glycerol and dibiphytanyl diglycerol, typical membrane lipids of Arch aea. From the neutral lipids, 2,3-di-O-dihydro-14,15-geranylgeranyl glycerol was isolated. This novel ether lipid is, very likely, a close intermediate in the biosynthesis of diphytanyl glycerol diether in Arch aea.
Tipo: Text Palavras-chave: Mass spectrometry; NMR; Novel glycerol diether lipid; Thermococcus sp; Archaea.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-736.pdf
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Análise e identificação das antocianinas de couve rôxa (Brassica spp.) IPB - Escola Superior Agrária
Lopes, Dulce; Jesus, Benvinda; Cabrita, Luís.
A couve rôxa é uma espécie comestível com uma composição em antocianinas rica e complexa. Sabe-se que contem antocianinas poliglicosiladas, aciladas com um ou mesmo dois grupos acilo aromáticos, nomeadamente com ácidos coumárico e caféico. Este padrão de acilação é particularmente interessante, porque confere estabilidade às antocianinas e aumenta o seu potencial de utilização em aplicações famacêuticas, alimentares e cosméticas. Este estudo tem por objectivo determinar a estrutura das antocianinas de variedades de couve rôxa cultivadas em Portugal, com vista ao seu aproveitamento industrial.
Tipo: ConferenceObject Palavras-chave: Anthocyanins; HPLC; NMR; Brassica spp; Red cabbage.
Ano: 2004 URL: http://hdl.handle.net/10198/3865
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Analysis of Water in the Chicken Eggshell Using the 1H Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy Rev. Bras. Ciênc. Avic.
Szeleszczuk,Ł; Pisklak,DM; Wawer,I.
ABSTRACT The water content of the chicken eggshell has a major influence on gas (CO2 and O2) permeability. Inappropriate water loss during the incubation period increases embryo mortality and decreases chick quality. So far only the procedures that enable to determine the total water content in the eggshell have been described and developed. Our analysis of the 1H MAS NMR spectra of the chicken eggshell samples revealed three signals, differing significantly in the chemical shift and relaxation times (T1) parameters. In this work we have assigned those signals and described the changes in their intensities that occur during the incubation period. Using 1H MAS NMR it is possible to distinguish two types of water reservoirs in the chicken eggshell. This...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Water; Chicken eggshell; NMR; Incubation.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000600027
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Bovine metabolomics: a NMR approach. Repositório Alice
POSTIGO, M. P.; CHAGAS, A. C. de S.; OLIVEIRA, M. C. de S.; COLNAGO, L. A..
2009
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: NMR.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/48900
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Characterisation of humic substances extracted from soil treated with charcoal. Repositório Alice
NOVOTNY, E. H.; AUCCAISE, R.; LIMA, L. B.; MADARI, B. E..
2012
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: BIOCHAR; PYROGENIC CARBON; HUMIC ACIDS; HUMIN; NMR; CHEMOMETRY.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/951535
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Complete assignments of NMR data and assessment of trypanocidal activity of new eremantholide C derivatives Anais da ABC (AABC)
SAÚDE-GUIMARÃES,DÊNIA A.; RASLAN,DÉLIO S.; CHIARI,EGLER; OLIVEIRA,ALAÍDE B. DE.
Chemical transformations of eremantholide C (1), a sesquiterpene lactone that was isolated from Lychnophora trichocarpha Spreng. led to five new derivatives: 1′,2′- epoxyeremantholide C (2), 5-n-propylamine-4,5-dihydro-1′,2′-epoxyeremantholide C (3), 5-n-propylammonium-4,5-dihydro-1′,2′-epoxyeremantholide C chloride (4), 5-n-propylammonium-4,5-dihydroeremantolide C chloride (5) and 16-O-ethyleremantholide C (6). The structures of all these derivatives were assigned on the basis of IR, MS, 1H and 13C NMR data by 1D and 2D techniques. Eremantholide C and the derivatives 2, 4 and 5 were evaluated against trypomastigotes Y and CL strains of Trypanosoma cruzi. Eremantholide C completely inhibited the growth of both the parasites strains while all derivatives...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eremantholide C derivatives; Lychnophora trichocarpha; NMR; Sesquiterpene lactones; Trypanocidal activity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401563
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Complete hydrolysis of myo-inositol hexakisphosphate by a novel phytase from Debaryomyces castellii CBS 2923 Inra
Ragon, M.; Aumelas, A.; Chemardin, P.; Galvez, S.; Moulin, G.; Boze, H..
Debaryomyces castellii phytase was purified to homogeneity in a single step by hydrophobic interaction chromatography. Its molecular mass is 74 kDa with 28.8% glycosylation. Its activity was optimal at 60 C and pH 4.0. The Km value for sodium phytate was 0.532 mM. The enzyme exhibited a low specificity and hydrolyzed many phosphate esters. The phytase fully hydrolyzed myo-inositol hexakisphosphate ( or phytic acid, Ins P-6) to inositol and inorganic phosphate. The sequence of Ins P-6 hydrolysis was determined by combining results from high-performance ionic chromatography and nuclear magnetic resonance. D. castellii phytase is a 3-phytase that sequentially releases phosphate groups through Ins ( 1,2,4,5,6) P-5, Ins ( 1,2,5,6) P-4, Ins ( 1,2,6) P-3, Ins (...
Tipo: Journal Article Palavras-chave: DEBAROMYCES CASTELLII; YEAST; PHYTASE; ENZYME CHARACTERIZATION; INOSITOL PHOSPHATE; PHYTATE DEGRADATION; NMR; HPLC.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009150d6498&uri=/notices/prodinra1/2010/09/
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Cytotoxic and Anti-Inflammatory Effects of Ent-Kaurane Derivatives Isolated from the Alpine Plant Sideritis hyssopifolia ArchiMer
Aimond, Axelle; Calabro, Kevin; Audoin, Coralie; Olivier, Elodie; Dutot, Mélody; Buron, Pauline; Rat, Patrice; Laprévote, Olivier; Prado, Soizic; Roulland, Emmanuel; Thomas, Olivier P.; Genta-jouve, Grégory.
This paper reports the isolation and structural characterization of four new ent-kaurane derivatives from the Lamiaceae plant Sideritis hyssopifolia. Planar structures and relative configurations were determined using both mass spectrometry and nuclear magnetic resonance (1D and 2D). Absolute configurations were determined by comparing experimental and theoretical electronic circular dichroism spectra. The cytotoxic and microbial activities of all new compounds were tested. Compounds that were non-cytotoxic were further evaluated for anti-inflammatory activity.
Tipo: Text Palavras-chave: Sideritis hyssopifolia; Ent-kaurane; Anti-inflammatory; NMR.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00610/72214/71012.pdf
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Distribution and mobility of phosphates and sodium ions in cheese by solid-state 31P and double-quantum filtered 23Na NMR spectroscopy Inra
Gobet, M.; Rondeau-Mouro, C.; Buchin, S.; Le Quéré, J.L.; Guichard, E.; Foucat, L.; Moreau, C..
The feasibility of solid-state magic angle spinning (MAS) 31P nuclear magnetic resonance (NMR) spectroscopy and 23Na NMR spectroscopy to investigate both phosphates and Na+ ions distribution in semi-hard cheeses in a non-destructive way was studied. Two semi-hard cheeses of known composition were made with two different salt contents. 31P Single-pulse excitation and cross-polarization MAS experiments allowed, for the first time, the identification and quantification of soluble and insoluble phosphates in the cheeses. The presence of a relatively mobile fraction of colloidal phosphates was evidenced. The detection by 23Na single-quantum NMR experiments of all the sodium ions in the cheeses was validated. The presence of a fraction of bound sodium ions was...
Tipo: Journal Article Palavras-chave: NMR; 23NA; 31P; DOUBLE-QUANTUM FILTER; MAGIC ANGLE SPINNING; SOLID STATE; BOUND SODIUM IONS; PHOSPHATE DISTRIBUTION; SEMI-HARD CHEESE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20109877c5f4&uri=/notices/prodinra1/2010/07/
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Evaluation of the cultivation age of dried ginseng radix and its commercial products by using 1H-NMR fingerprint analysis Taiwan Agricultural Research Institute
Lin, W. N.; Lu, H. Y. ; Lee, M. S. ;Yang, S. Y. ; Chen, H. J. ; Chang, Y. S.;Chang, W. T..
Palavras-chave: Ginseng; Cultivation Age; Metabolomic; NMR; Principal Component Analysis; Cluster Analysis [[classification]]6.
Ano: 2010
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Extraction and characterization of organic fractions isolated from a China Ball Clay quarried in Devon Sout West England. Repositório Alice
McINERNEV, R.; STUART, J.; NOVOTNY, E. H.; HAYES, M. H. B.; YOUNG, K..
2008
Tipo: Artigo em anais de congresso (ALICE) Palavras-chave: China Ball Clay; Organic fraction; Humic; NMR.
Ano: 2008 URL: http://www.alice.cnptia.embrapa.br/handle/doc/334712
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Genotype evaluation of cowpea seeds (Vigna unguiculata) using 1H qNMR combined with exploratory tools and solid-state NMR. Repositório Alice
ALVES FILHO, E. G.; SILVA, L. M. A. e; TEOFILO, E. M.; LARSEN, F. H.; BRITO, E. S. de.
bitstream/item/167028/1/ART17019.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Genótipo de sementes de caupi; Oligosacarídeos; NMR; Chemometria; CP-MAS; SP/MAS; Cowpea seeds genotype; Chemometrics; Oligosaccharides.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1073517
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Glutathionylation induces the dissociation of 1-Cys D-peroxiredoxin non-covalent homodimer Inra
Noguera-Mazon, V.; Lemoine, J.; Walker, O.; Rouhier, N.; Salvador, A.; Jacquot, J.P.; Lancelin, J.M.; Krimm, I..
1-Cys peroxiredoxins (1-Cys Prxs) are antioxidant enzymes that catalyze the reduction of hydroperoxides into alcohols using a strictly conserved cysteine. 1-Cys B-Prxs, homologous to human PrxVI, were recently shown to be reactivated by glutathione S-transferase (GST) {pi} via the formation of a GST-Prx heterodimer and Prx glutathionylation. In contrast, 1-Cys D-Prxs, homologous to human PrxV, are reactivated by the glutaredoxin-glutathione system through an unknown mechanism. To investigate the mechanistic events that mediate the 1-Cys D-Prx regeneration, interaction of the Prx with glutathione was studied by mass spectrometry and NMR. This work reveals that the Prx can be glutathionylated on its active site cysteine. Evidences are reported that the...
Tipo: Journal Article Palavras-chave: PEROXIREDOXINE; HYDROPEROXYDE; CYSTEINE; RMN; RESONANCE MAGNETIQUE NUCLEAIRE PEROXIREDOXIN; HYDROPEROXIDE; CYSTEINE; NMR; SPECTROMETRY.
Ano: 2006 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20074bcd41f3&uri=/notices/prodinra1/2010/11/
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High resolution magic angle spinning and solid-state NMR spectroscopy methods to explore the metabolome of soybean genotypes upon abiotic stress Repositório Alice
COUTINHO, I. D.; HENNING, L. M. M.; NEPOMUCENO, A,; SANTAGNELID, S.; COLNAGO, L. A..
bitstream/item/169628/1/P-High-Resolution-Magic-Angle-....pdf
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: HR MAS; NMR; Soybean; Metabolome.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1083288
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Increasing the detection distance of remote NMR using wireless inductive coupling coil. Repositório Alice
KILLNER, M. H. M.; TOSIN, G.; CARVALHO, A. S.; BERNARDES, D. F.; COLNAGO, L. A..
bitstream/item/166380/1/PROCI-Increasing-the-detection....pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Unilateral nuclear magnetic resonance; NMR; Petrochemistry; Materials science; Process control.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079179
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Lessons from the Terra Preta de Índios of the Amazon region for the utilisation of charcoal for soil amendment. Repositório Alice
NOVOTNY, E. H.; HAYES, M. H. B.; MADARI, B. E.; BONAGAMBA, T. J.; AZEVEDO, E. R. de; SOUZA, A. A. de; SONG, G.; NOGUEIRA, C. M.; MANGRICH, A. S..
Neste trabalho faz-se a divulgação do potencial de carvões e resíduos orgânicos parcialmente carbonizados visando obter materiais que mimetizam a matéria orgânica do solo das Terras Pretas de Índio da Amazônia, e que sirvam como condicionadores de solo e seqüestrem carbono de forma recalcitrante e reativa. Pesquisas desenvolvidas por grupos brasileiros e estrangeiros têm contribuído para o entendimento do surgimento e utilização das Terras Pretas de Índio da Amazônia. Aqui são divulgados resultados de estudos químicos no sentido do desenvolvimento do conhecimento científico e tecnológico e de inovação no aproveitamento de subprodutos orgânicos, principalmente de indústrias de biocombustíveis, carvão vegetal metalúrgico e outros, buscando imitar a excelente...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Terra preta de índios; Amazônia; NMR; Carbono; Charcoal; Metabolic sequestration; Nuclear magnetic resonance spectroscopy.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/659439
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Letter to the Editor: 1H, 13C and 15N NMR assignment of the homodimeric poplar phloem type II peroxiredoxin Inra
Bouillac, S.; Rouhier, N.; Tsan, P.; Jacquot, J.P.; Lancelin, J.M..
Tipo: Journal Article Palavras-chave: DEUTERATION; GLUTAREDOXINE; HYDROPEROXIDE; RMN; RESONANCE MAGNETIQUE NUCLEAIRE; PEROXIREDOXINE; PLANTE DEUTERATION; GLUTAREDOXIN; HYDROPEROXIDE; NMR; PEROXIREDOXIN; PLANT.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007238dad88&uri=/notices/prodinra1/2010/11/
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Métabolisme énergétique du spermatozoïde d'huître creuse (Crassostrea gigas) ArchiMer
Boulais, Myrina.
The present study aims at describing the energy metabolism of Pacific oyster, Crassostrea gigas, spermatozoon. The main objectives are: (1) describing the kinetic of cellular and biochemical characteristics of Pacific oyster spermatozoon during its movement phase, (2) identifying the metabolic pathways to produce energy and their substrates, (3) determining the involvement of energy metabolism in fertilization success. The results showed the long duration (ranging 24 to 72h) of the movement of Pacific oyster spermatozoon and the maintenance of a high intracellular ATP content until the end of the movement phase. The role of oxidative phosphorylation (OXPHOS) in ATP production was highlighted. Alternative ATP producing pathways might contribute to sustain...
Tipo: Text Palavras-chave: Fécondation; Sperme; Analyse du mouvement; Métabolisme énergétique; RMN; Cytométrie en flux; Crassostrea gigas; Reproduction; Fertilization; Sperm; Movement analysis; Energetic metabolism; NMR; Flow cytometry.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00498/60940/64334.pdf
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Metabolomics as a promising tool for early osteoarthritis diagnosis BJMBR
de Sousa,E.B.; dos Santos Junior,G.C.; Duarte,M.E.L.; Moura Neto,V.; Aguiar,D.P..
Osteoarthritis (OA) is the main cause of disability worldwide, due to progressive articular cartilage loss and degeneration. According to recent research, OA is more than just a degenerative disease due to some metabolic components associated to its pathogenesis. However, no biomarker has been identified to detect this disease at early stages or to track its development. Metabolomics is an emerging field and has the potential to detect many metabolites in a single spectrum using high resolution nuclear magnetic resonance (NMR) techniques or mass spectrometry (MS). NMR is a reproducible and reliable non-destructive analytical method. On the other hand, MS has a lower detection limit and is more destructive, but it is more sensitive. NMR and MS are useful...
Tipo: Info:eu-repo/semantics/article Palavras-chave: NMR; Metabolomics; Osteoarthritis; Synovial Fluid.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100301
Registros recuperados: 39
Primeira ... 12 ... Última
 

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