SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Aborto bovino por Neospora caninum no Rio Grande do Sul

Dubey, J. P..

Neospora caninum is a recently recognized protozoan parasite of animals. Until 1988, it was misdiagnosed as Toxoplasma gondii. Neospora caninum or Neospora-like parasites cause paralysis and death in dogs and neonatal mortality and abortion in cattle, sheep, goats and horses. Its life cycle is not known. Tachyzoites and tissue cysts are the only asexual stages known and the carnivorous definitive host is not known. Transplacental transmission is the only known natural route of infection.

Palavras-chave: Neospora caninum; Neosporosis; Tachyzoites; Tissue cysts; Paralysis; Abortion.

Ano: 1992

URL: http://ir.obihiro.ac.jp/dspace/handle/10322/163

Corbellini,Luís Gustavo; Driemeier,David; Cruz,Cláudio; Dias,Marcelo Maronna.

Casos de aborto bovino submetidos ao Setor de Patologia da Faculdade de Veterinária da Universidade Federal do Rio Grande do Sul entre os anos de 1995 e o primeiro trimestre de 1999 foram selecionados e analisados histologicamente. Um total de 30 fetos abortados foram avaliados através da coloração de Hematoxilina e Eosina (HE) e lesões inflamatórias não supurativas foram encontradas principalmente no cérebro e/ou coração de seis casos. Realizou-se imunoistoquímica pela técnica de streptavidina-biotina, utilizando-se anticorpos anti-Neospora caninum e anti-Toxoplasma gondii nesses seis casos. Taquizoítos marcados pelo anticorpo anti-N. caninum foram visualizados em três dos seis fetos bovinos testados e não houve reação com o anticorpo anti-T. gondii....

Anticorpos anti-Neospora caninum em bovinos de leite do sudoeste do estado do Paraná

Palavras-chave: Neospora caninum; Aborto; Bovino; Imunoistoquímica.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000500021

Anticorpos anti-Neospora caninum em bovinos, ovinos e bubalinos no Estado do Rio Grande do Sul

Camillo,G; Cadore,G; Cezar,A.S; Toscan,G; Bräunig,P; Sangioni,L.A; Vogel,F.S.F.

Since November 2007 until May 2009, 1,778 serum samples of cattle from dairy herds of the Southwest of Paraná State, Brazil, were used for search of anti-Neospora caninum antibodies. The frequency of seropositive animals, assessed by IFAT, was 24.2% (431/1,178), showing a relatively high occurrence in the studied population. These results show that Neospora caninum is widely distributed in the dairy cattle in the Southwest of Paraná State. The presence of Neospora caninum in a herd is worrisome, since this protozoan is closely related with reproductive disorders and low milk production in cattle.

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Bovino; Neospora caninum; Prevalência; Imunofluorescência indireta.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600033

Vogel,Fernanda Silveira Flôres; Arenhart,Sandra; Bauermann,Fernando Viçosa.

A infecção pelo Neospora caninum é distribuída mundialmente e tem sido considerada uma importante causa de abortos em bovinos, que são hospedeiros intermediários do protozoário. O presente artigo relata um estudo sorológico da infecção pelo N. caninum em 1.024 amostras de bovinos, ovinos e bubalinos, oriundos de 55 propriedades em 16 municípios do Estado do Rio Grande do Sul (RS). Anticorpos contra o agente foram detectados por um teste imunoenzimático do tipo ELISA em 11,4% (89/781) das amostras de bovinos, em 14,6% (24/164) dos bubalinos e em 3,2% (2/62) dos ovinos. Bovinos soropositivos foram detectados em todos os municípios amostrados. Esses resultados demonstram que a infecção pelo N. caninum encontra-se amplamente difundida no rebanho bovino e...

Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites

Palavras-chave: Neospora caninum; Sorologia; Epidemiologia; Bovinos; Bubalinos; Ovinos.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000600048

Associação entre sorologia para Neospora caninum e taxa de prenhez em vacas receptoras de embriões

Zhang, Houshuang; Compaore, Muller K.A.; Lee, Eung-goo; Liao, Min; Zhang, Guohong; Sugimoto, Chihiro; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan.

The cross-reactive antigens of Neospora caninum and Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite-host interaction. In this study, a gene encoding N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera. from mice immunized with recombinant T gondii apical membrane antigen 1 (TgAMA 1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by...

Palavras-chave: Neospora caninum; Toxoplasma gondii; Apical membrane antigen 1; Cross-reactive; Invasion.

Ano: 2007

URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1034

Paz,G.F.; Leite,R.C.; Rocha,M.A..

The association between seropositivity for Neospora caninum and pregnancy rate in cows belonging to a surrogate herd submitted to embryo transfer technology was determined. The serological status was evaluated in 275 heifers, aging from 14 to 20-month-old. For N. caninum serology analysis of a monoclonal competitive ELISA test Kit was used, and 81 animals (29.5%) showed seropositive. Thus, two groups were randomly formed selecting 33 seropositive heifers and other 33 seronegative animals out of the remaining 194 animals. Seronegative animals were followed up by serological analysis until the end of the trial in order to identify persistently infected individuals. The pregnancy rate was 72.7% in the group of N. caninum-positive sera, and, 81.8% in the...

Canine distemper virus and Toxoplasma gondii co-infection in dogs with neurological signs

Palavras-chave: Bovino; Neospora caninum; Transferência de embriões; Taxa de prenhez.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500034

Aguiar,D.M.; Amude,A.M.; Santos,L.G.F.; Ribeiro,M.G.; Ueno,T.E.H.; Megid,J.; Paes,A.C.; Alfieri,A.F.; Alfieri,A.A.; Gennari,S.M..

O presente estudo relata a ocorrência de co-infecção entre o vírus da cinomose canina (CDV) e Toxoplama gondii em cães com sinais neurológicos. Amostras de soro e tecido nervoso (pos-mortem) de 21 cães, suspeitos de cinomose canina foram analisadas pela Reação de Imunofluorecência indireta (RIFI) para pesquisa de anticorpos contra T. gondii e N. caninum e por RT-PCR para CDV. Dezessete (80,9%) cães foram positivos para o CDV pela RT-PCR e 8 (38,1%) foram positivos para anticorpos contra T. gondii. Sete cães (41,1%) apresentaram-se positivos para ambos agentes, caracterizando processo de co-infecção. Somente 1 (4,7%) cão foi soropositivo para N. caninum (RIFI=100), entretanto este mesmo animal foi positivo para T. gondii (RIFI=4096) e para CDV (RT-PCR).

Dairy cow abortion associated with Neospora caninum and other infectious agents

Palavras-chave: Dogs; Canine distemper; Toxoplasmosis; Neospora caninum; Co-infection.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000100032

Santos,A.P.M.E.; Navarro,I.T.; Bracarense,A.P.F.R.L.; Freire,R.L.; Marana,E.R.M.; Ogawa,L.; Alfieri,A.A.; Freitas,J.C.; Vidotto,O..

Trinta e cinco vacas de rebanhos leiteiros da região Norte do estado do Paraná, com histórico de abortamento, foram pesquisadas sorologicamente para verificar a presença de anticorpos contra Neospora caninum, Toxoplasma gondii, Leptospira spp., Brucella abortus, BHV-1 e BVDV. Vinte e uma vacas apresentaram títulos sorológicos compatíveis com infecção. Todas elas, soropositivas para N. caninum, foram também soropositivas para outros agentes infecciosos, sugerindo a possibilidade de associação desses agentes nos problemas reprodutivos de bovinos, no estado do Paraná.

Detección de Neospora caninum en fetos bovinos abortados espontáneamente en el nordeste argentino

Palavras-chave: Cattle; Abortion; Neospora caninum; Reproductive disorder.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000400017

InVet

Lertora,W.J.; Mohr Betiana,N.; Mosqueda,M.G.; Sánchez Negrette,M..

El objetivo del presente trabajo fue detectar Neospora caninum en neonatos y fetos bovinos de abortos espontáneos del nordeste argentino. Sobre un total de 26 fetos bovinos abortados espontáneamente (23 de cría y 3 de tambo) se detectaron lesiones histopatológicas compatibles con neosporosis en 4 de ellos. La presencia de Neospora caninum fue confirmada mediante inmunohistoquímica en el encéfalo de 3/4 fetos (2 de cría y 1 de tambo) con lesiones microscópicas compatibles con neosporosis. Las lesiones histopatológicas más relevantes fueron la asociación de encefalitis, miocarditis y hepatitis no purulenta multifocales. De 10 neonatos bovinos procesados (8 de cría y 2 de tambo), no se detectaron animales con lesiones compatibles con neosporosis. Este trabajo...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Neospora caninum; Aborto bovino; Histopatología; Inmunohistoquímica.

Ano: 2010

URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982010000200007

Effects of Thai piperaceae plant extracts on Neospora caninum infection

Leesombun, Arpron; Boonmasawai, Sookruetai; Nishikawa, Yoshifumi.

Neosporosis has a worldwide distribution and causes economic losses in farming, particularly by increasing the risk of abortion in cattle. This study investigated the effects of Thai piperaceae (Piper betle, P. nigrum, and P. sarmentosum) extracts on Neospora caninum infections in vitro and in vivo. In an in vitro parasite growth assay based on the green fluorescent protein (GFP) signal, P. betle was the most effective extract at inhibiting parasite growth in human foreskin fibroblast cells (IC50 of GFP-expressing N. caninum parasites, 22.1 mu g/ml). The P. betle extract, at 25 mu g per ml, inhibited parasite invasion into host cells. Furthermore, in two independent experiments, treating N. caninum-infected mice with the P. betle extract for 7 days...

Palavras-chave: Neospora caninum; Neosporosis; Piper betle; Piper nigrum; Piper sarmentosum; Ethanol extract.

Ano: 2017

URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4578

ENCUESTA SEROLÓGICA DE AGENTES INFECCIOSOS, RELACIONADOS CON ABORTOS EN VACAS LECHERAS

Rev Salud Anim.

Sánchez-Castilleja,Yolanda M; Pedroso,Miriam; Rodríguez Diego,J.G.

Con el objetivo de realizar una pesquisa serológica de agentes infecciosos causantes de abortos en vacas lecheras Holstein en etapa de producción pertenecientes a la Cuenca Lechera de Tizayuca, Hgo., México, se tomaron 261 muestras para análisis por ELISA indirecto para la determinación de anticuerpos contra Neospora caninum, los virus IBR, PI3, RVSB y la prueba de tarjeta para Brucella abortus. Los resultados obtenidos evidencian la circulación de anticuerpos contra Neospora caninum (48.65%), PI3 (44.44%), RVSB (35. 43%), IBR (35.18%) y Brucella abortus (32.18%), que indican la necesidad de establecer programas de prevención y control, así como realizar el diagnóstico diferencial en productos del aborto.

Tipo: Journal article

Palavras-chave: Neospora caninum; IBR; PI3; RVSB; Abortos; Evaluación; Serológica.

Ano: 2008

URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2008000100011

Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey

Estimating the abortion risk difference in Neospora caninum seropositive dairy cattle in Brazil

Zhou, Mo; Cao, Shinuo; Sevinc, Ferda; Sevinc, Mutlu; Ceylan, Onur; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Suzuki, Hiroshi; Nishikawa, Yoshifumi; Xuan, Xuenan.

Considering the scarce information on occurrences of Toxoplasma gondii and Neospora caninum in domestic animals from Turkey, the aim of this study was to investigate the seroprevalence of these parasite infections in cattle, horses, sheep, goats and dogs in Turkey. The specific antibodies against T gondii and N. caninum were detected by iELISAs based on the recombinant TgSAG2 or NcSAG1 in a total of 2,039 serum samples from eleven provinces. The seroprevalence of T. gondii infections was 46.3%, 4.0%, 20.0%, 12.9% and 19.8%, that of N. caninum infections was 0.3%, 7.4%, 2.1%, 3.2% and 16.6% in the horses, cattle, sheep, goats and dogs, respectively. These results indicated that T gondii and N. caninum infections are prevalent in Turkish domestic animals.

Palavras-chave: ELISA; Neospora caninum; Toxoplasma gondii; Turkey.

Ano: 2016

URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4422

Nicolino,Rafael Romero; Capanema,Renato Oliveira; Oliveira,Camila Stefanie Fonseca de; Pastrana,Misael Enrique Oviedo; Lopes,Luciano Bastos; Haddad,João Paulo Amaral.

Neosporosis in cattle herds is associated with large economic losses, with abortion being the only clinical sign perceptible to the producer. Losses are estimated at over one billion dollars worldwide. This study aimed to estimate the abortion risk difference in seropositive animals using specific data for dairy herds in Brazil. Differences in the risk of abortion between seropositive and seronegative animals were calculated through a meta-analysis of previous data from several Brazilian states, and an increase of 10.04% (0.091 to 0.118) in the specific risk was identified. This finding indicates that more than 474,000 abortions caused by neosporosis may be occurring only in dairy cattle herds in Brazil, causing a major economic loss in the milk production...

Estudio retrospectivo de agentes infecciosos que afectan la reproducción bovina en el departamento del Caquetá, Colombia

Palavras-chave: Abortion risk; Neospora caninum; Dairy herd; Economic modeling; Brazil.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000901629

Rev Salud Anim.

Motta Giraldo,J.L; Waltero García,Indira; Abeledo,Ma. Antonia; Fernández,O.

Se analizaron los resultados de las investigaciones serológicas para brucelosis, leptospirosis, herpesvirus bovino -1 (HVB-1), diarrea viral bovina (DVB) y Neospora caninum, de los casos enviados al laboratorio por los propietarios de los hatos bovinos en el departamento del Caquetá, Colombia, en los años 1999 - 2009. La información fue obtenida de la base de datos del ICA (Instituto Colombiano Agropecuario), seccional Caquetá. Se consideraron las variables: agentes infecciosos, años, municipios, zonas y sexos. El porcentaje promedio de brucelosis fue de 6,4% y los mayores valores se obtuvieron para herpesvirus bovino-1 (HVB-1). En el caso de Neospora, las investigaciones comenzaron en 2007 y en los 3 años solo se trabajaron 36 muestras, de las cuales 12...

Tipo: Journal article

Palavras-chave: Brucellosis; Leptospirosis; Herpesvirus bovino-1; Diarrea viral bovina; Neospora caninum.

Ano: 2012

URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000300004

Etiologic diagnosis of bovine infectious abortion by PCR

Silva,Teane Milagres Augusto da; Oliveira,Raquel Gonzaga de; Mol,Juliana Pinto da Silva; Xavier,Mariana Noyma; Paixão,Tatiane Alves da; Cortez,Adriana; Heinemann,Marcos Bryan; Richtzenhain,Leonardo José; Lage,Andrey Pereira; Santos,Renato de Lima.

Infectious abortion is a significant cause of reproductive failure and economic losses in cattle. The goal of this study was to detect nucleic acids of several infectious agents known to cause abortion including Arcanobacterium pyogenes, Bovine Herpesvirus 1, Brucella abortus, Campylobacter fetus subsp. venerealis, Chlamydophila abortus, Leptospira sp., Listeria monocytogenes, Salmonella sp., Mycoplasma bovis, Mycoplasma bovigenitalium, Neospora caninum, and Tritrichomonas foetus. Tissue homogenates from 42 fetuses and paraffin-embedded tissues from 28 fetuses and 14 placentas/endometrium were included in this study. Brucella abortus was detected in 14.2% (12/84) of the samples. Salmonella sp. DNA was amplified from 2 fetuses, and there was one positive...

Expression of recombinant dense granule protein 7 of Neospora caninum and evaluation of its diagnostic potential for canine neosporosis

Palavras-chave: Cattle; Abortion; Brucella abortus; Salmonella sp.; Neospora caninum; Listeria monocytogenes.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900028

Freqüência de cães reagentes para Neospora caninum em São Luís, Maranhão

Hara, Olgga A; Liao, Min; Baticados, Waren; Bannai, Hiroshi; Zhang, Guohong; Zhang, Sofa; Lee, Eung-goo; Nishikawa, Yoshifumi; Claveria, Florencia G.; Igarashi, Makoto; Nagasawa, Hideyuki; Xuan, Xuenan.

Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were...

Palavras-chave: Canine; ELISA; Neospora caninum; NcGRA7.

Ano: 2006

URL: http://ir.obihiro.ac.jp/dspace/handle/10322/995

Teixeira,W.C.; Silva,M.I.S.; Pereira,J.G.; Pinheiro,A.M.; Almeida,M.A.O.; Gondim,L.F.P..

The frequency of antibodies to Neospora caninum (Protozoa: Apicomplexa) in dog sera was investigated. Blood samples from 100 mongrel dogs, captured in the streets of São Luís, State of Maranhão, were analized using imunofluorescent antibody test. Forty five percent of the dogs were positive, and the titers ranged from 1:50 to 1:800. No sex difference was observed for frequency of N. caninum (60% in males and 40% in females). This is the first report of antibodies to N. caninum in dogs from Maranhão.

Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii

Palavras-chave: Cão; Freqüência; Neospora caninum; Imunoflorescência indireta; Maranhão.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000400038

Identification of ribosomal phosphoprotein P0 of Neospora caninum as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis

Liao, Min; Xuan, Xuenan; Huang, Xiaohong; Shirafuji, Hiroaki; Fukumoto, Shinya; Hirata, Haruyuki; Suzuki, Hiroshi; Fujisaki, Kozo.

Murine monoclonal antibodies (mAbs) against Neospora caninum tachyzoites were produced to identify the cross-reactive antigens between N. caninum and Toxoplasma gondii. Ten mAbs recognizing cross-reactive antigens of both parasites were obtained and tentatively classified into 6 different groups based on their reactivity patterns in an indirect fluorescent antibody test and Western blot analysis. Three mAbs in group I recognized antigens located on the surface of parasites with molecular masses ranging from 28 to 76 kDa; one mAb in group 2 recognized antigens located on interior organelles of parasites with a molecular mass of 50 kDa; one mAb in group 3 recognized antigens located on interior organelles of parasites with molecular masses of 35 kDa and 14...

Palavras-chave: Neospora caninum; Toxoplasma gondii; Cross-reactive antigen; Protein disulfide isomerase; Heat-shock protein; Ribosomal protein 1.

Ano: 2005

URL: http://ir.obihiro.ac.jp/dspace/handle/10322/816