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| Registros recuperados: 14 | |
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| Nakaghi,Andréa Cristina Higa; Machado,Rosangela Zacarias; Costa,Mirela Tinucci; André,Marcos Rogério; Baldani,Cristiane Divan. |
| The aim of the present study was to compare the direct detection methods of Ehrlichia canis (blood smears and nested PCR), serological tests (Dot-ELISA and Immunofluorescent Antibody Test - IFAT), and demonstrate the most suitable test for the diagnosis of different stages of infection. Blood samples and clinical data were collected from 30 dogs examined at the Veterinary Teaching Hospital, UNESP, Jaboticabal, SP, Brazil. The clinical signs most frequently observed were apathy, anorexia, pale mucous membrane, fever, lymphadenopathy, splenomegaly, hemorrhages and uveitis. Evaluating the humoral immune response, 63.3% of the sera were IFAT positive, while 70% were Dot-ELISA positive. By nestedPCR 53.3% of the samples were positive. Comparing these techniques... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ehrlichia canis; Dog; IFAT; Dot-ELISA; Nested PCR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000300027 |
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| Mares-Guia,Maria Angélica M.M.; Guterres,Alexandro; Rozental,Tatiana; Ferreira,Michelle dos Santos; Lemos,Elba R.S.. |
| ABSTRACT Q fever is a worldwide zoonosis caused by Coxiella burnetii—a small obligate intracellular Gram-negative bacterium found in a variety of animals. It is transmitted to humans by inhalation of contaminated aerosols from urine, feces, milk, amniotic fluid, placenta, abortion products, wool, and rarely by ingestion of raw milk from infected animals. Nested PCR can improve the sensitivity and specificity of testing while offering a suitable amplicon size for sequencing. Serial dilutions were performed tenfold to test the limit of detection, and the result was 10× detection of C. burnetti DNA with internal nested PCR primers relative to trans-PCR. Different biological samples were tested and identified only in nested PCR. This demonstrates the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Q fever; Coxiella burnetii; Molecular diagnosis; Nested PCR; IS1111. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100138 |
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| Makino,Herica; Sousa,Valéria Régia Franco; Fujimori,Mahyumi; Rodrigues,Juliana Yuki; Dias,Alvaro Felipe Lima Ruy; Dutra,Valéria; Nakazato,Luciano; Almeida,Arleana do Bom Parto Ferreira de. |
| ABSTRACT: The objective of this study was to compare the DNA detection of Ehrlichia canis in blood and bone marrow to determine the prevalence of the agent in Várzea Grande, Mato Grosso. Blood samples and bone marrow from 80 dogs of both sexes, different breeds and age, were collected and processed for a cross-sectional study performed using nested PCR. Of the 80 dogs, 61 (76.3%) had E. canis DNA in one of the samples. The buffy coat was positive in 42 dogs (52.5%) and the bone marrow was positive in 33 (41.3%). There was no significant association between the positive biological samples of either the buffy coat or bone marrow and the presence or absence of clinical signs (P=0.49). No risk factor was associated with infection in the studied area. The bone... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine ehrlichiosis; Diagnosis; Nested PCR; Biological samples.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200310 |
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| Tozetti,Inês Aparecida; Scapulatempo,Ilzia Doraci Lins; Kawski,Vicky Liege; Ferreira,Antonio Walter; Levi,José Eduardo. |
| We analyzed 87 cervical samples from Campo Grande, Mato Grosso do Sul, with a PGMY/GP+ nested PCR system. Positive samples were typed using E7 type-specific primer pairs for HPV 6/11, 16, 18, 45 and 66. Eighteen samples (22%) were infected with HPV6/11, 18 samples (22%) with HPV66, 13 samples (15.9%) with HPV45, 8 samples (9.8%) with HPV18 and 7 samples (8.5%) with HPV16. Seventeen samples (20.7%) were infected by two HPV types, and five samples (6.1%) by three HPV types. We conclude that infection with multiple types is present at a high frequency in our population and that there is a relation between some types and cytological finds. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human papillomavirus; Multiple types; Nested PCR; TS-PCR. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500001 |
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| Giacomazzi, Sophie; Leroi, Francoise; L'Henaff, Cecile; Joffraud, Jean-jacques. |
| Aims: To evaluate rpoB gene as a biomarker of microbial biodiversity associated to cold-smoked salmon by a novel nested-polymerase chain reaction/temporal temperature gradient gel electrophoresis (PCR/TTGE) technique applied on pure cultures of reference strains. Methods and Results: DNA obtained from pure cultures of reference strains was used in a succession of a first PCR amplification of rpoB fragment with degenerated nonclamped primers and a nested-PCR with nondegenerated clamped primers. PCR products were then applied on a TTGE gel in order to analyse strains profile. High quantity of nested-PCR products were obtained for each tested strain and TTGE profiles showed a good separation between the different reference bacteria and an easy way to... |
| Tipo: Text |
Palavras-chave: TTGE; RpoB; Nested PCR; Cold smoked salmon flora. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-579.pdf |
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| Costa,L.F.; Nozaki,C.N.; Lira,N.S.C.; Antunes,J.M.A.P.; Xavier,M.N.; Costa,E.A.; Paixão,T.A.; Santos,R.L.; Megid,J.. |
| The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams. The performance of the species-specific nested PCR was compared with the results of a genus-specific PCR. Fourteen rams were experimentally infected with the Brucella ovis REO 198 strain and samples of semen and urine were collected every week up to 180 days post infection. Out of 83 semen samples collected, 42 (50.6%) were positive for the species-specific nested PCR, and 23 (27.7%) were positive for the genus-specific PCR. Out of 75 urine samples, 49 (65.3%) were positive for the species-specific nested PCR, whereas 11 (14.6%) were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella ovis; Species-specific; Nested PCR; Semen; Urine. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100009 |
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| ÁVILA, A. A.; SIDER, L. H.; VERAS, A. K. A.; PINHEIRO, R. R.; OLIVEIRA, M. L. M.; SILVA, P. A. F.; SOUSA, S. D.; ANDRIOLI, A.. |
| Resumo: Neste estudo, 67 ejaculados foram avaliados, antes e depois da técnica de swim-up, em relação à qualidade seminal e à presença do CAEV. Das 67 amostras testadas por PCRn, antes do swim-up, 47 (70,15%) foram positivas para o DNA pró-viral. No entanto, quatro amostras adicionais foram positivas ao RT-nested PCR após o swim-up, o que permite dizer que, pelo menos, 76,12% (51/67) delas estavam infectadas antes da lavagem. Todavia, em 23,88% (16/67) das amostras não foi detectada a presença do CAEV. Após a aplicação da técnica de swim-up, constatou-se, pela PCRn e RT-nested PCR, que houve uma redução significativa (X²= 9,078; p<0,001) da presença do CAEV nas amostras seminais, pois 28 de 51 amostras positivas resultaram livres do vírus (54,90%),... |
| Tipo: Artigo de periódico |
Palavras-chave: CAEV; PCRn; Lavagem seminal; Artrite Encefalite Caprina; Seminal washing; Nested PCR; RT-nested PCR; Percoll.; Caprino; Sêmen; DNA.; Goats.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032730 |
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| Rivera,Vanessa; Gaviria,Marcela; Muñoz-Cadavid,Cesar; Cano,Luz; Naranjo,Tonny. |
| ABSTRACT The diagnosis of cryptococcosis is usually performed based on cultures of tissue or body fluids and isolation of the fungus, but this method may require several days. Direct microscopic examination, although rapid, is relatively insensitive. Biochemical and immunodiagnostic rapid tests are also used. However, all of these methods have limitations that may hinder final diagnosis. The increasing incidence of fungal infections has focused attention on tools for rapid and accurate diagnosis using molecular biological techniques. Currently, PCR-based methods, particularly nested, multiplex and real-time PCR, provide both high sensitivity and specificity. In the present study, we evaluated a nested PCR targeting the gene encoding the ITS-1 and ITS-2... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptococcus neoformans/Cryptococcus gattiicomplex; Nested PCR; Cryptococcosis; Molecular diagnosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600563 |
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| Registros recuperados: 14 | |
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