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Implementation of a Method to Determine Lolitrem-B in Ryegrass (Lolium perenne L.) by Liquid Chromatography (HPLC) Chilean J. Agric. Res.
Moyano A,Stella; Lanuza,Francisco; Torres B,Alfredo; Cisternas A,Ernesto; Fuentes V,Marcela.
Lolitrem-B is a neurotoxic substance that causes the ryegrass staggers syndrome in cattle, sheep, and horses consuming ryegrass (Lolium perenne L.) infected with endophytic fungus Neotyphodium lolii (Latch, Christensen, and Samuels) Glenn, Bacon, and Hanlin. Due to the increasing use of ryegrass pasture in southern Chile, it is necessary to know the content of lolitrem-B to optimize grazing management and reduce the effect on animal health. The objective of this study was to implement a method to determine lolitrem-B in ryegrass. Ryegrass samples were used without the endophyte, freeze-dried, and kept in darkness at -18 ºC to establish the method. Then, a pure lolitrem-B standard was added and the toxin was extracted with a mixture of chloroform-methanol...
Tipo: Journal article Palavras-chave: Mycotoxin; Neurotoxin; Endophyte fungi.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300019
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Partial purification and functional characterization of Ts19 Frag-I, a novel toxin from Tityus serrulatus scorpion venom J. Venom. Anim. Toxins incl. Trop. Dis.
Lima,Priscila C.; Bordon,Karla C. F.; Pucca,Manuela B.; Cerni,Felipe A.; Zoccal,Karina F.; Faccioli,Lucia H.; Arantes,Eliane C..
Abstract Background The yellow scorpion Tityus serrulatus (Ts) is responsible for the highest number of accidents and the most severe scorpion envenoming in Brazil. Although its venom has been studied since the 1950s, it presents a number of orphan peptides that have not been studied so far. The objective of our research was to isolate and identify the components present in the fractions VIIIA and VIIIB of Ts venom, in order to search for a novel toxin. The major isolated toxins were further investigated for macrophage modulation. Methods The fractions VIIIA and VIIIB, obtained from Ts venom cation exchange chromatography, were rechromatographed on a C18 column (4.6 × 250 mm) followed by a reversed-phase chromatography using another C18 column (2.1 ×...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-KTx; Cytokine; Interleukin; Neurotoxin; Pro-inflammatory; Scorpion venom; Tityus serrulatus.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100353
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