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Registros recuperados: 16
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Antimicrobial peptides and nitric oxide production by neutrophils from periodontitis subjects BJMBR
Mariano,F.S.; Campanelli,A.P.; Nociti Jr.,F.H.; Mattos-Graner,R.O.; Gonçalves,R.B..
Neutrophils play an important role in periodontitis by producing nitric oxide (NO) and antimicrobial peptides, molecules with microbicidal activity via oxygen-dependent and -independent mechanisms, respectively. It is unknown whether variation in the production of antimicrobial peptides such as LL-37, human neutrophil peptides (HNP) 1-3, and NO by neutrophils influences the pathogenesis of periodontal diseases. We compared the production of these peptides and NO by lipopolysaccharide (LPS)-stimulated neutrophils isolated from healthy subjects and from patients with periodontitis. Peripheral blood neutrophils were cultured with or without Aggregatibacter actinomycetemcomitans-LPS (Aa-LPS), Porphyromonas gingivalis-LPS (Pg-LPS) and Escherichia coli-LPS...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial peptides; Nitric oxide; Neutrophils; Periodontitis; Innate immunity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100004
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Chloramphenicol restores the polymorphonuclear accumulation in carrageenin-induced pleurisy in diabetic rats Braz. J. Vet. Res. Anim. Sci.
MORAES,Julieta Rodini Engrácia de; MORAES,Flávio Ruas de; ENGRÁCIA FILHO,Jair Rodini.
We investigated the effect of chloramphenicol succinate (30 mg/kg, every 12 h for 4 days, ip) on the reduced polymorphonuclear leukocytes (PMN) accumulation in carrageenin-induced pleurisy (150 mug) in diabetic (alloxan, 40 mg/kg, IV) rats (Wistar, males, 180-320 g, n = 12). Chloramphenicol produced an increase (p<0.05) on PMN count in pleural exudate of 36% in normal animals. In the animals non-treated with chloramphenicol the diabetes state reduced (p<0.05) the accumulation of PMN cells by 45%. In diabetic animals treated with chloramphenicol the number of PMN in pleural exudate was similar to the one observed in the control group. Total and differential counts of leukocytes in the peripheral blood were similar between groups before and 4 h after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chloramphenicol; Diabetes; Pleurisia; Neutrophils.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000600002
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Cytotoxic activity of the dichloromethane fraction from Vernonia scorpioides (Lam.) Pers. (Asteraceae) against Ehrlich's tumor cells in mice BJMBR
Pagno,T.; Blind,L.Z.; Biavatti,M.W.; Kreuger,M.R.O..
Vernonia scorpioides has been widely used in Brazil to treat skin problems and chronic wounds, such as ulcers of the lower limbs and diabetic lesions. In the present study, we investigated the effect of a dichloromethane (DCM) fraction of V. scorpioides leaf extract on Ehrlich ascitic and solid tumor-bearing mice. The animals were treated once a day with the DCM fraction at a concentration of 5 mg/kg, administered ip during and after the development of the tumor. The lifespan, weight, number and type of leukocytes, number of tumor cells, volume of solid and ascitic tumors were measured. The development of the tumor with pre-treated tumor cells in vitro with the DCM fraction (5 mg/kg) was analyzed and the animals were sacrificed after 7 days. The DCM...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vernonia scorpioides; Leaf extract; Antitumoral; Ehrlich ascitic tumor; Neutrophils.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100012
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Effect of acupuncture on TNF-alpha, IL-1b and IL-10 concentrations in the peritoneal exudates of carrageenan-induced peritonitis in rats Ciência Rural
Scognamillo-Szabó,Márcia Valéria Rizzo; Bechara,Gervásio Henrique; Cunha,Fernando de Queiroz.
Acupuncture is an ancient and empirical therapeutic procedure known by its efficacy in the treatment of pain. However, the influence of acupuncture on inflammatory process is still poorly understood and additional research is needed. In this work, we investigated the mechanism of action of manual acupuncture on the inhibition of neutrophil migration to the peritoneal cavity induced by the inflammatory stimulus carrageenan in Wistar rats. Previous results from our laboratory showed that this anti-inflammatory effect is not due to endogenous corticoid release. Furthermore, the concentration of IL-<FONT FACE=Symbol>1b</FONT>, but not of TNF-alpha or IL-10 in the carrageenan-induced exudates was reduced in the acupuncture group. Further research...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acupuncture; Inflammation; Cytokines; Neutrophils; Rats; Carrageenan.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000100016
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Effect of pentoxifylline on lung inflammation and gas exchange in a sepsis-induced acute lung injury model BJMBR
Oliveira-Junior,I.S.; Brunialti,M.K.C.; Koh,I.H.J.; Junqueira,V.B.C.; Salomão,R..
Experimental models of sepsis-induced pulmonary alterations are important for the study of pathogenesis and for potential intervention therapies. The objective of the present study was to characterize lung dysfunction (low PaO2 and high PaCO2, and increased cellular infiltration, protein extravasation, and malondialdehyde (MDA) production assessed in bronchoalveolar lavage) in a sepsis model consisting of intraperitoneal (ip) injection of Escherichia coli and the protective effects of pentoxifylline (PTX). Male Wistar rats (weighing between 270 and 350 g) were injected ip with 10(7) or 10(9) CFU/100 g body weight or saline and samples were collected 2, 6, 12, and 24 h later (N = 5 each group). PaO2, PaCO2 and pH were measured in blood, and cellular influx,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acute lung injury; Sepsis; Inflammatory mediators; Pentoxifylline; Neutrophils.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006001100009
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Effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs on the production of reactive oxygen species by activated rat neutrophils BJMBR
Paino,I.M.M.; Ximenes,V.F.; Fonseca,L.M. da; Kanegae,M.P.P.; Khalil,N.M.; Brunetti,I.L..
The release of reactive oxygen specie (ROS) by activated neutrophil is involved in both the antimicrobial and deleterious effects in chronic inflammation. The objective of the present investigation was to determine the effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs (NSAIDs) on the production of ROS by stimulated rat neutrophils. Diclofenac (3.6 µM), indomethacin (12 µM), naproxen (160 µM), piroxicam (13 µM), and tenoxicam (30 µM) were incubated at 37ºC in PBS (10 mM), pH 7.4, for 30 min with rat neutrophils (1 x 10(6) cells/ml) stimulated by phorbol-12-myristate-13-acetate (100 nM). The ROS production was measured by luminol and lucigenin-dependent chemiluminescence. Except for naproxen, NSAIDs reduced ROS production:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Non-steroidal anti-inflammatory drugs; Neutrophils; Myeloperoxidase; Hypochlorous acid; Reactive oxygen species; Chemiluminescence.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400007
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Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia Biol. Res.
TIIDUS,PETER M; DELLER,MIRADA; BOMBARDIER,ERIC; GÜL,MUSTAFA; LIU,X. LINDA.
This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sacrificed at 24, 48 or 72 hours post-ischemia. Control animals were sacrificed without ischemia. Plantaris and red and white gastrocneimus muscles were removed and assayed for myeloperoxidase (MPO), a marker of neutrophil infiltration, and glucose-6-phosphate dehydrogenase (G6PD) and ß-glucuronidase (<img border=0 width=8 height=15 id="_x0000_i1026" src="http://fbpe/img/bres/v38n2-3/existe.gif" align=absbottom>GLU), as markers of muscle damage. Significant...
Tipo: Journal article Palavras-chave: Ischemia; Estrogen; Muscle damage; Skeletal muscle; Neutrophils.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200011
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Evidence for the participation of nitric oxide in pemphigus BJMBR
Siebra,M.X.; Santos,M.A.; Almeida,T.L.P.; Leite,A.C.R.M.; Cunha,F.Q.; Rocha,F.A.C..
Pemphigus is an inflammatory autoimmune disorder of the skin. Nitric oxide (NO) is an inflammatory mediator linked to a variety of physiological and pathophysiological phenomena that include skin tumors, psoriasis, urticaria, and atopic dermatitis. Inflammatory cells present in pemphigus lesions are important sources of NO production. We investigated whether NO is involved in pemphigus. A prospective cohort study was conducted at the Dermatology Service of the Hospital Universitário Walter Cantídio of the Federal University of Ceará. All patients seen at the outpatient clinic between August 2000 and July 2001, with a clinically and histologically confirmed diagnosis of pemphigus were included. The median age was 42.5 years (range: 12-69 years) with a male...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Autoimmunity; Inflammation; Neutrophils; Nitric oxide; Pemphigus; Inducible nitric oxide synthase.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000500014
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Lipopolysaccharide-induced expression of cell surface receptors and cell activation of neutrophils and monocytes in whole human blood BJMBR
Gomes,N.E.; Brunialti,M.K.C.; Mendes,M.E.; Freudenberg,M.; Galanos,C.; Salomão,R..
Lipopolysaccharide (LPS) activates neutrophils and monocytes, inducing a wide array of biological activities. LPS rough (R) and smooth (S) forms signal through Toll-like receptor 4 (TLR4), but differ in their requirement for CD14. Since the R-form LPS can interact with TLR4 independent of CD14 and the differential expression of CD14 on neutrophils and monocytes, we used the S-form LPS from Salmonella abortus equi and the R-form LPS from Salmonella minnesota mutants to evaluate LPS-induced activation of human neutrophils and monocytes in whole blood from healthy volunteers. Expression of cell surface receptors and reactive oxygen species (ROS) and nitric oxide (NO) generation were measured by flow cytometry in whole blood monocytes and neutrophils. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipopolysaccharide; Neutrophils; Monocytes; Cell surface receptors; Reactive oxygen species; Nitric oxide.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900007
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Metabolic fate of glutamine in lymphocytes, macrophages and neutrophils BJMBR
Curi,R.; Newsholme,P.; Pithon-Curi,T.C.; Pires-de-Melo,M.; Garcia,C.; Homem-de-Bittencourt Jr.,P.I.; Guimarães,A.R.P..
Eric Newsholme's laboratory was the first to show glutamine utilization by lymphocytes and macrophages. Recently, we have found that neutrophils also utilize glutamine. This amino acid has been shown to play a role in lymphocyte proliferation, cytokine production by lymphocytes and macrophages and phagocytosis and superoxide production by macrophages and neutrophils. Knowledge of the metabolic fate of glutamine in these cells is important for the understanding of the role and function of this amino acid in the maintenance of the proliferative, phagocytic and secretory capacities of these cells. Glutamine and glucose are poorly oxidized by these cells and might produce important precursors for DNA, RNA, protein and lipid synthesis. The high rate of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutamine; Lymphocytes; Macrophages; Neutrophils; Metabolic fate.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100002
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Neutrophil function and metabolism in individuals with diabetes mellitus BJMBR
Alba-Loureiro,T.C.; Munhoz,C.D.; Martins,J.O.; Cerchiaro,G.A.; Scavone,C.; Curi,R.; Sannomiya,P..
Neutrophils act as first-line-of-defense cells and the reduction of their functional activity contributes to the high susceptibilityto and severity of infections in diabetes mellitus. Clinical investigations in diabetic patients and experimental studies in diabetic rats and mice clearly demonstrated consistent defects of neutrophil chemotactic, phagocytic and microbicidal activities. Other alterations that have been reported to occur during inflammation in diabetes mellitus include: decreased microvascular responses to inflammatory mediators such as histamine and bradykinin, reduced protein leakage and edema formation, reduced mast cell degranulation, impairment of neutrophil adhesionto the endothelium and migration to the site of inflammation, production...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neutrophils; Inflammation; Diabetes mellitus; Insulin; Metabolism and cellular function.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800003
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Role of previous stimulation on the neutrophil migration protects against the lethal effect of Salmonella typhimurium Braz. J. Vet. Res. Anim. Sci.
MORAES,Flávio Ruas de; KLEIN Jr.,Manoel Henrique; MORAES,Julieta Rodini Engrácia de.
The intraperitoneal inoculation of 3.8 x 10(9) CFU of alive Salmonella typhimurium caused 100% mortality in rats. In these animals a small number of neutrophils migrated to the peritoneal cavity. The intraperitoneal inoculation of the animals with thioglycollate (Tg, 90 mg), carrageenin (Cg, 500 mu g), or killed S. typhimurium (KS, 1.9 x 10(9) CFU) 24 hours before challenge with alive S. typhimurium caused a significant neutrophil migration to the peritoneal cavity (determined 6 hours after challenge) and protected the animals against the lethal effect of the bacteria at a rate of 50%, 83% and 80%, respectively. However, similar treatment of the animals with brain heart infusion broth (BHI, S. typhimurium grout medium) protected the animals by only 23%....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Migration; Neutrophils; Salmonella typhimurium; Peritonitis; Septicemia; Phagocytosis.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000100002
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Structure and function of the selectin ligand PSGL-1 BJMBR
Cummings,R.D..
P-selectin glycoprotein ligand-1 (PSGL-1) is a dimeric mucin-like 120-kDa glycoprotein on leukocyte surfaces that binds to P- and L-selectin and promotes cell adhesion in the inflammatory response. The extreme amino terminal extracellular domain of PSGL-1 is critical for these interactions, based on site-directed mutagenesis, blocking monoclonal antibodies, and biochemical analyses. The current hypothesis is that for high affinity interactions with P-selectin, PSGL-1 must contain O-glycans with a core-2 branched motif containing the sialyl Lewis x antigen (NeuAc<FONT FACE="Symbol">a</font>2<FONT FACE="Symbol">®</font>3Galß1<FONT FACE="Symbol">®</font>4[Fuc<FONT FACE="Symbol">a</font>1<FONT...
Tipo: Info:eu-repo/semantics/article Palavras-chave: P-selectin; L-selectin; E-selectin; PSGL-1; O-glycosylation; Glycoprotein; Mucin; Tyrosine sulfate; Cell adhesion; Leukocytes; Neutrophils.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500004
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The use of reverse transcription-PCR for the diagnosis of X-linked chronic granulomatous disease BJMBR
Agudelo-Flórez,P.; López,J.A.; Redher,J.; Carneiro-Sampaio,M.M.S.; Costa-Carvalho,B.T.; Grumach,A.S.; Condino-Neto,A..
Chronic granulomatous disease (CGD) is an inherited disorder of the innate immune system characterized by a defective oxidative burst of phagocytes and subsequent impairment of their microbicidal activity. Mutations in one of the NADPH-oxidase components affect gene expression or function of this system, leading to the phenotype of CGD. Defects in gp91-phox lead to X-linked CGD, responsible for approximately 70% of CGD cases. Investigation of the highly heterogeneous genotype of CGD patients includes mutation analysis, Northern blot or Western blot assays according to the particular case. The aim of the present study was to use reverse transcription (RT)-PCR for the analysis of molecular defects responsible for X-linked CGD in eight Brazilian patients and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superoxide; Phagocytes; Primary immunodeficiency; Respiratory burst; Neutrophils; Human.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500001
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Treatment of hemorrhagic shock with hypertonic saline solution modulates the inflammatory response to live bacteria in lungs BJMBR
Fernandes,C.I.; Llimona,F.; Godoy,L.C.; Negri,E.M.; Pontieri,V.; Moretti,A.I.; Fernandes,T.R.; Soriano,F.G.; Velasco,I.T.; Souza,H.P..
Shock and resuscitation render patients more susceptible to acute lung injury due to an exacerbated immune response to subsequent inflammatory stimuli. To study the role of innate immunity in this situation, we investigated acute lung injury in an experimental model of ischemia-reperfusion (I-R) followed by an early challenge with live bacteria. Conscious rats (N = 8 in each group) were submitted to controlled hemorrhage and resuscitated with isotonic saline (SS, 0.9% NaCl) or hypertonic saline (HS, 7.5% NaCl) solution, followed by intratracheal or intraperitoneal inoculation of Escherichia coli. After infection, toll-like receptor (TLR) 2 and 4 mRNA expression was monitored by RT-PCR in infected tissues. Plasma levels of tumor necrosis factor α and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toll-like receptors; Immune system; Pneumonia; Escherichia coli; Neutrophils.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001000004
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Ureases display biological effects independent of enzymatic activity: Is there a connection to diseases caused by urease-producing bacteria? BJMBR
Olivera-Severo,D.; Wassermann,G.E.; Carlini,C.R..
Ureases are enzymes from plants, fungi and bacteria that catalyze the hydrolysis of urea to form ammonia and carbon dioxide. While fungal and plant ureases are homo-oligomers of 90-kDa subunits, bacterial ureases are multimers of two or three subunit complexes. We showed that some isoforms of jack bean urease, canatoxin and the classical urease, bind to glycoconjugates and induce platelet aggregation. Canatoxin also promotes release of histamine from mast cells, insulin from pancreatic cells and neurotransmitters from brain synaptosomes. In vivo it induces rat paw edema and neutrophil chemotaxis. These effects are independent of ureolytic activity and require activation of eicosanoid metabolism and calcium channels. Helicobacter pylori, a Gram-negative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Urease; Canatoxin; Helicobacter pylori; Inflammation; Neutrophils; Eicosanoids.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700002
Registros recuperados: 16
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