|
|
|
|
| |
|
|
| Scire, A.; Marabotti, A.; Staiano, M.; Briand, L.; Varriale, A.; Bertoli, E.; Tanfani, F.; D’Auria, S.. |
| The effect of temperature on the structure of the rat odorant-binding protein was investigated by spectroscopic and in silico methodologies. In particular, in this work, we examined the structural features of the rat OBP-1F by Fourier-transform infrared spectroscopy and molecular dynamics investigations. The obtained spectroscopic results were analyzed using the following three different methods based on the unexchanged amide hydrogens of the protein sample: (1) the analysis of difference spectra; (2) the generalized 2D-IR correlation spectroscopy; (3) the phase diagram method. The three methods indicated that at high temperatures the rOBP-1F structure undergoes a relaxation process involving the protein tertiary organization before undergoing the... |
| Tipo: Journal Article |
Palavras-chave: ODORANT-BINDING PROTEIN; FOURIER TRANSFORM INFRARED SPECTROSCOPIE; LIPOCALIN. |
| Ano: 2009 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010bef21c07&uri=/notices/prodinra1/2010/07/ |
| |
|
|
|
