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| Registros recuperados: 43 | |
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| De Sousa, Joana Teixeira; Milan, Massimo; Pauletto, Marianna; Bargelloni, Luca; Joaquim, Sandra; Matias, Domitilia; Matias, Ana Margarete; Quillien, Virgile; Leitao, Alexandra; Huvet, Arnaud. |
| A microarray-based analysis was performed with the objective of describing genomic features of oocytes and looking for potential markers of oocyte quality in the economically important European clam, Ruditapes decussatus. Oocytes of 25 females from Ria de Aveiro, Western coast of Portugal (40°42′N; 08°40′W) were selected for this study and oocyte quality was estimated by success of D-larval rate under controlled conditions, which appeared to vary from 0 to 95 %. By genome-wide expression profiling with a DNA microarray, 526 probes appeared differentially expressed between two groups representing the largest and smallest value of D larval rates, named good (represented by a mean D-larval yield of 57 ± 22%) and poor (9 ± 5%) quality oocytes. Enrichment... |
| Tipo: Text |
Palavras-chave: Ruditapes decussatus; Oocyte; Developmental success; Gene expression; Marine bivalve; Transcriptomics. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00260/37147/35676.pdf |
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| Leal,C.L.V.; Méo,S.C.; Garcia,J.M.. |
| O objetivo deste trabalho foi avaliar as taxas de ativação e de clivagem de oócitos bovinos tratados com estrôncio (10mm de SrCl2), após maturação in vitro por 27-28 horas. No experimento 1, os tratamentos foram: S4 - ativação pelo estrôncio por 4 horas; S12 - ativação pelo estrôncio por 12 horas; S30 - ativação pelo estrôncio por 30 horas; e P - ativação por pulso elétrico (3 pulsos de 1,0kv/cm). No experimento 2 os tratamentos foram: PS4 - ativação combinada pelo pulso elétrico e pelo estrôncio por 4 horas; S4P - ativação pelo estrôncio por 4 horas e pelo pulso elétrico; e PS30 - ativação pelo pulso elétrico e pelo estrôncio por 30 horas. No experimento 1, todos os tratamentos apresentaram taxas similares de ativação (83-90%; P>0,05). Para clivagem, P... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cattle; Activation; Parthenogenesis; Oocyte; Strontium; Electric pulse. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300020 |
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| Tsai,S.; Lin,C.. |
| Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking. Cryopreservation of fish embryo is not viable, mainly because of the same limitations as in fish oocytes, i.e., high chilling sensitivity and low membrane permeability. However, cryopreservation of isolated embryonic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryopreservation; Sperm; Embryo; Oocyte; Blastomere. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300014 |
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| Adona,P.R.; Leal,C.L.V.. |
| The effect of concentration and exposure period of bovine oocytes to butyrolactone I (BLI) on meiotic block and in vitro maturation (IVM) kinetics was studied. In experiment 1, all oocytes were at germinal vesicle stage (GV), after 6h in culture with 0, 50 and 100µM BLI. After 12h, all oocytes cultured with 50 and 100µM BLI remained in GV. After 24h, less oocytes were in GV with 50µM (82%) than with 100µM BLI (99%, P<0.05). In experiment 2, after 6h IVM, 93% of control oocytes (IVM only) were in GV, while treated oocytes (100µM BLI for 6, 12 or 24h prior to IVM) showed less oocytes in GV with increased exposure period to BLI prior to IVM (83 and 73%, for 6h and 12h, P<0.05). For a 24h inhibition, GV rates were similar to 12h (70%, P>0.05). After... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Meiosis; Oocyte; Butyrolactone I; Kinetics; Nuclear maturation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300011 |
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| Santana,B.B.; Sobral,G.G.; Gomes,E.T.; Batista,A.M.; Teixeira,L.P.R.; Tavares,K.C.S.; Bertolini,M.; Carneiro,G.F.. |
| ABSTRACT The aim of this work was to evaluate the effect of the Rolipram during the maturation of bovine oocytes and gene expression of embryos produced in vitro. Bovine ovaries were collected in slaughterhouse. The COCs were selected and divided into 5 groups: Control 0 time; Control: IVM for 24 hours; Rolipram treatments with IVM blocking for 24 hours in maturation medium containing (100, 150 and 200µM). After 24 hours all groups were reseated in IVM for another 24 hours. Subsequently COCs were subjected to the same IVM system and fertilized, being checked for cleavage post fertilization and for blastocyst. In addition, performed expression of the following genes: Mater, BMP15 and Bax. No difference was found in gene expression. Of oocytes evaluated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Rolipram; IVF; IVM; Oocyte. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501433 |
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| SALES, J. N. S.; IGUMA, L. T.; BATISTA, R. I. T. P.; QUINTAO, C. C. R.; GAMA, M. A. S. da; FREITAS, C. de; PEREIRA, M. M.; CAMARGO, L. S. de A.; VIANA, J. H. M.; SOUZA, J. C.; BARUSELLI, P. S.. |
| Tipo: Separatas |
Palavras-chave: Bovine; Oocyte; Ovum pick-up in vitro production.; Cattle; Nutrition.. |
| Ano: 2015 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035432 |
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| Bombardelli,Robie Allan; Sanches,Eduardo Antônio; Baggio,Diego Mendes; Sykora,Renata Marino; Souza,Bruno Estevão de; Tessaro,Lucélia; Piana,Pitágoras Augusto. |
| The objective of this study was to evaluate the effects of water volume and water temperature on the sperm motility duration and the number of spermatozoa, and the water volume on the fertilization rates of oocytes of Rhinelepis aspera. Experiments were carried out to evaluate the effect of semen dilutions (1.74×10-5, 1.74×10-4, 1.74×10-3, 1.74×10-2, 1.74×10-1 and 1.00 mL of sperm.mL-1 of water) and water temperature (5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 ºC) on spermatozoa motility duration. In addition, the effects of insemination dose (7×10³, 7×10(4), 7×10(5), 7×10(6) and 7×10(7) spermatozoa.oocyte-1) and water volume (1.0, 30.0, 60.0, 90.0 and 120.0 mL water.2.0 mL-1 oocytes) on the artificial fertilization rates of oocytes were evaluated. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish; Loricariidae; Oocyte; Reproduction; Semen. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000100001 |
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| Leitão,Cintia Camurça Fernandes; Costa,José Jackson Nascimento; Saraiva,Márcia Viviane Alves; Araújo,Valdevane Rocha; Figueiredo,José Ricardo; Hurk,Robert van den; Silva,José Roberto Viana. |
| This study investigated the levels of messenger ribonucleic acids (mRNA) for inhibin-ßA subunit in goat primordial, primary and secondary follicles, as well as in cumulus-oocyte complexes (COCs) and mural granulosa / theca cells of antral follicles. The effects of activin-A (100ng mL-1) and/or follicle stimulating hormone (FSH, 50ng mL-1) on growth and expression of mRNA for activin-A and FSH receptor (FSH-R) in secondary follicles cultured for six days were evaluated. The data showed that the expression of inhibin-ßA is lower in secondary follicles than in primary follicles and is higher in large antral follicles than in small antral follicles. After culture, activin-A and/or FSH promoted growth of secondary follicles, while FSH increased the levels of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pre-antral follicles; Caprine; Culture; Oocyte; Antrum formation; FSH-R. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100018 |
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| Araújo,Francisco G.; Nascimento,Aparecida A.; Gomes,Iracema D.; Sales,Armando; Oliveira,Beatriz A. Chagas de. |
| ABSTRACT The gonadal development of the characin Astyanax aff. bimaculatus from Funil Reservoir, an impoundment in the middle reaches of the Paraiba do Sul River in southeastern Brazil was analysed through histological, histochemical and histometric techniques, and the spawning season was determined. Unbalanced sex ratio was found, with females outnumbering and reaching larger sizes than males. The following stages of oogenic cell development were identified for females: primary growth, pre-vitellogenic (cortical alveolar) and vitellogenic. In males, the following stages were determined: spermatogonia primary and secondary, spermatocyte primary and secondary, spermatid and spermatozoa. The spawning occurs most of the year, peaking in January-February, as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Freshwater fish; Histology; Oocyte; Spawning type; Spermatocyte. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702019000100326 |
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| Rosa,Paulo Roberto Antunes da; Bohrer,Rodrigo Camponogara; Ludke,Charles Alencar; Cesaro,Matheus Pedroti De; Pereira,Gabriel Ribas; Mondadori,Rafael Gianela; Antoniazzi,Alfredo Quites; Gonçalves,Paulo Bayard Dias. |
| The objective of this study was to investigate the mRNA expression and protein localization of Grb10 gene in bovine cumulus-oocyte complexes (COCs) from different follicle sizes. Firstly, it was investigated the mRNA expression to correlate with maturation rates. COCs from follicles at 1-3, 4-6, 6-8 and >8mm were used to evaluate Grb10 gene expression by qRT-PCR assay and nuclear maturation rates. It was observed that more competent oocytes (from follicles at 6-8 and >8mm; P>0.05), had lower Grb10 mRNA expression levels when compared to the oocytes from follicles at 1-3 and 4-6mm (P>0.05). After it was performed an immunofluorescence analysis in COCs from different follicle sizes (1-3, 4-6, 6-8 and >8mm) to investigate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: : mRNA; Protein; Maturation; Oocyte; Grb10.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500898 |
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| Dupré,Enrique M; Barros,Claudio. |
| This review compiles all the research done on gametes and fertilization in the rock shrimp, R. typus, and describes the sequence of events from the first gamete interaction to zygote formation and the first cleavage of the embryo, with light, fluorescence confocal and electron microscopes. Early studies showed that sperm from the vas deferens have a tack-shape with a "needle-like process" or rigid spike (RS) that extends from a semi-spherical body that contains the arms with chromatin and spines. Upon contact with seawater and by action of Na +, the arms and spines extend, producing an inverted umbrella form of the spermatozoa. The first sperm-oocyte interaction occurs between protein receptors type lectins of the sperm RS and oocyte chorion sperm ligands.... |
| Tipo: Journal article |
Palavras-chave: Spermatic ultrastructure; Oocyte; Sperm-egg interaction. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200003 |
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| Comtet, T; Garcia, Celine; Le Coguic, Yvette; Joly, Jean-pierre. |
| We report the occurrence of the microsporidian parasite Steinhausia sp. in the oocytes of the common cockle Cerastoderma edule in a natural population in France, where high mortalities occurred. Steinhausia sp. appeared primarily as sporocysts containing many small spores, and putative earlier developmental stages were also observed. Both its prevalence and infection intensity were low, and no host defence reaction was recognized, suggesting that Steinhausia sp. had no detrimental effect on C. edule. Its prevalence was higher in cockles lying on the sediment surface, but the significance of this observation could not be explained given the poor knowledge of the Steinhausia life cycle. The present data did not allow specific identification of the parasite,... |
| Tipo: Text |
Palavras-chave: Cerastoderma edule; Steinhausia sp.; Microsporida; Oocyte; Baie des veys. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00000/10762/7586.pdf |
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| Barros,F.F.P.C.; Teixeira,P.P.M.; Uscategui,R.A.R.; Coutinho,L.N.; Brito,M.B.S.; Kawanami,A.E.; Almeida,V.T.; Mariano,R.S.G.; Nociti,R.P.; Machado,M.R.F.; Vicente,W.R.R.. |
| ABSTRACT The aim of this work is study the laparoscopic ovum pick-up (LapOPU) technique in spotted paca, describing surgery details, complications and oocyte recovery rate. Nine healthy adult non-pregnant captive females were used, in a total of 39 procedures. When the surgical plane of anaesthesia was achieved, the females were positioned at 20º Trendelenburg. Three 6mm trocars were placed on right and left inguinal and hypogastric regions. Abdomen was inflated with CO2 and the intra-abdominal pressure was stablished in 10mmHg. Follicular punctures were performed moving the ovaries with atraumatic forceps. For punctures, an 18-gauge 3.5 inch long needle attached to a vacuum system with pressure not exceeding 65mmHg was used. Oocytes were recovered into... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Follicular aspiration; Laparoscopy; Oocyte; Rodent; Wild animals. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000400858 |
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| Registros recuperados: 43 | |
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