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| Registros recuperados: 23 | |
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| FUJlHIRA, Takuma; KINOSHITA, Mikio; SASAKI, Motoki; OHNISHI, Masao; ISHIKAWA, Hajime; OHSUMI, Seiji; FUKUI, Yutaka; 木下, 幹朗; 佐々木, 基樹; 大西, 正男. |
| The present study was conducted to clarify the difference in the color of the cytoplasm in immature follicular oocytes from prepubertal and adult minke whales. The four lipid contents (triglyceride，total cholesterol phospholipids and non-esterified fatty acids) in vitrified immature oocytes from prepubertal and adult minke whales，and also in fresh and vitrified immature porcine oocytes，were measured. The lipid contents in vitrified-warmed minke whale oocytes were similarly high compared with those in vitrified-warmedp orcine oocytes. In particular，the total cholesterol and phospholipid contents in the vitrified immature oocytes from prepubertal and adult minke whales were significantly( P<0.05) higher than those from prepubertal pigs. Furthermore，the... |
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Palavras-chave: Minke whale; Porcine; Oocytes; Lipid; Sexual maturity. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2731 |
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| Cruz,Maria Helena Coelho; Saraiva,Naiara Zoccal; Cruz,Jurandir Ferreira da; Oliveira,Clara Slade; Del Collado,Maite; Fernandes,Hugo; Castro,Fernanda Cavallari de; Garcia,Joaquim Mansano. |
| This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo development; Inner cell mass; In vitro fertilization; Oocytes. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300003 |
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| Oliveira,Classius de; Santos,Lia Raquel de Souza. |
| This paper describes morphological characteristics of the ovarian germinative cells of the Scinax fuscovarius (Lutz, 1925). The ovary is organized in primordial germinative cells (oogonia) and follicular structures (ovarian follicle) - oocytes surrounded by follicular cells. Oogonia: their nests are peripherically localized, containing cells with large and oval nucleus. Oocytes I: basophilic cytoplasm; the spherical nucleus presents few nucleoli, one or two; the follicular cells (one tenuous layer) surround these previtellogenic cells. Oocytes II: the cell is larger and the cytoplasm becomes more basophilic; the nucleus presents few nucleoli and contains many chromosomes in the periphery (beginning the perinucleolar stage). Oocytes III: the cytoplasm... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oocytes; Oogonia; Ovary; Pigment; Vitellogenesis. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752004000400029 |
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| Pessoa,G.A; Navarro,R.B; Rauber,L.P; Brass,K.E; Silva,C.A.M; Rubin,M.I.B. |
| Temperature and rainfall were analyzed daily during six years to evaluate their influence on in vitro production of bovine embryos. Weekly replications (n=480) were performed on 14,778 ovaries collected at slaughterhouses. Cumulus oocyte complexes (n=19,180) were fertilized with a pool of Bos taurus taurus semen in one incubator with 5% CO2. Presumable zygotes were cultured in gasified plastic bags with 5% CO2, 5% O2, and 90% N2. In the first year, cleavage and embryo yield were 60.3% and 15.6%, respectively, being lower (P<0.05) than in the following years. Average cleavage rates were always lower in winter (P<0.0001), thus producing less embryos. Winter climatic conditions had a negative influence on in vitro production, when cleavage and embryo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cow; Oocytes; Embryo; Climate; In vitro prodution. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600013 |
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| Sanches,Eduardo Antônio; Caneppele,Danilo; Okawara,Renan Yoshiharu; Damasceno,Danielle Zanerato; Bombardelli,Robie Allan; Romagosa,Elizabeth. |
| Abstract The Steindachneridion parahybae is an endangered catfish from Brazil and strategies applied for gametes optimization are necessary. The aim of this study was to assess inseminating doses and water volume upon the fertilization, hatching rates and percentage of normal larvae in S. parahybae . Was used a randomized design in factorial scheme (4×4) with four inseminating doses: 1.0×104, 1.0×105, 1.0×106, 1.0×107spermatozoa oocyte-1 and four volumes of water: 1, 35, 65 and 95mL of water g-1 of oocytes. The combination of doses and volumes were performed in triplicates (n=48). Each incubator (1.5L of useful volume) with 1g of oocytes was considered as an experimental unit. Significant interaction between inseminating doses and volumes of water to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artificial reproduction; Freshwater fish; Oocytes; Sperm; Surubim-do-Paraíba. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000100204 |
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| Max, Marilu Constantino; Andrade, Evelyn Rabelo de; Basso, Andrea Cristina; de Figueiredo, José Ricardo; Seneda, Marcelo Marcondes. |
| Objective: To disclose a new biotechnique, listing some of its aspects and addressing its main stages, isolation, cryopreservation and culture of preantral follicles. Data Sources: The information was obtained from CAB, MEDLINE, PUBMED, Science Direct and CAPES Portal. Data Synthesis: The preantral follicles store about 90% of the oocytes within the ovary. The biotechnique described is based on recovering immature oocytes retained inside preantral follicles before atresia takes place. The cryopreservation of these cells enables the creation of germoplasm banks, preserving the genetic material of women submitted to chemotherapy and/or radiotherapy, as well as that endangered animal species. Conclusions: Being a recent biotechnique, its application is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biotechonology; Reproductive techniques; Oocytes; Transplantation; Ovarianfollicles; Cryopreservation Biotecnologia; Técnicas reproductivas; Oocitos; Transplantación; Foliculo ovárico; Criopreservación biotecnologia; Técnicas reprodutivas; Oócitos; Transplante; Folículo ovariano; Criopreservação. |
| Ano: 2004 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3239 |
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| Navarrete Gómez,Patricia; Ormeño,David; Miranda,Antonio; Sánchez Gutiérrez,Raúl; Romero Mejía,Fernando; Parodi Rivera,Jorge. |
| Since the 1970s, There have been studies of the venom of Latrodectus sp. spiders, in particular the latrotoxin (LTX) of Latrodectus mactans. Many of the studies were aimed at understanding the action of the venom on the muscular system. Now accepted that LTX is able to generate a calcium-permeable membrane pore and modulate the release of synaptic vesicles that activate a receptor and induce cellular changes. Interestingly, when work began with venom obtained from the Latrodectus sp present in Chile, it generated clinical indications similar to the bite of this spider in another country, with some differences in intensity. The purpose of the first studies was to understand the systemic mechanisms of this venom, and other active compounds were studied for... |
| Tipo: Journal article |
Palavras-chave: Latrodectus venom; Oocytes; Neurons; Spermatozoa; Contraceptive. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300012 |
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| Melo, Gabriele Sauthier Romano de; Santana, Herick Soares de; Tos, Claudenice Dei. |
| The construction of the Itaipu Hydroelectric Power Plant in 1982 led to the formation of a reservoir, which, in turn, leveled the waters of the Paraná River by flooding the geographic barrier Salto de Sete Quedas. This allowed the piranha Serrasalmus marginatus to invade and colonize the upper Paraná River. This study aimed to: i) confirm, through light microscopy, the reproductive phases of S. marginatus females; ii) estimate fecundity and iii) evaluate the reproduction areas of the population. A total of 764 females were collected from nine sampling sites on the upper Paraná River floodplain. Microscopic analysis of the ovaries showed the following phases: early developing subphase, developing phase, spawning capable phase, actively spawning subphase,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.05.02.00-1 Indeterminate fecundity; Type of spawning; Oogenesis; Oocytes; Piranha. Ecologia de Ecossistemas. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33021 |
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| Fernandes,Cezar A. F.; Oliveira,Paulo G. V. de; Travassos,Paulo E. P.; Hazin,Fábio H. V.. |
| Reproductive aspects of the Brazilian snapper Lutjanus alexandrei, were characterized, including a description of the development of oocytes and spermatogenic cells, size at first sexual maturity, and fecundity. A total of 540 fish were analyzed with 250 having their gonads sectioned to allow microscopic evaluation. Six maturity stages were identified for females and males: immature, maturing, mature, spawning, spawned, and resting. Fish standard length (SL) varied from 13.0 to 28.3 cm and sex ratio was 1.6 males: 1.0 females. Monthly distributions of mean Gonadosomatic Index (GSI) and maturity stages suggest that spawning occurs mainly in a protracted period, during the warmer months, from November to March. The size of first sexual maturity was estimated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Multiple spawning; Oocytes; Sexual maturity; Spermatogenic cells. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300012 |
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| Lima,G.L.; Santos,E.A.A.; Lima,L.F.; Luz,V.B.; Rodrigues,A.P.R.; Silva,A.R.. |
| We compare protocols for the short-term preservation of collared peccarie's ovarian preantral follicles (PFs) by using phosphate buffered saline- (PBS) or powdered coconut water- (ACP(r)) based medium. For morphology analysis each pair of ovaries collected from six females was divided into nine fragments. One fragment was destined for morphology analysis (histology and transmission electron microscopy - TEM), constituting the control group and the other fragments were placed in tubes with PBS or ACP(r), packed in 5 L Styrofoam boxes, stored for 4h, 12h, 24h, and 36h, and then analyzed. For viability analysis a pair of ovaries from two additional females was divided into nine fragments; one fragment was immediately destined for viability analysis (Trypan... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Collared peccaries; Preantral follicles; Coconut water; Ovary; Oocytes. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601623 |
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| Dutertre, Mickael; Beninger, Peter G.; Barille, Laurent; Papin, Mathias; Rosa, Philippe; Barille, Anne-laure; Haure, Joel. |
| The proliferation of the voluntarily-introduced cupped oyster, Crassostrea gigas, has attained the proportions of species invasion in many intertidal habitats in Europe, presumably resulting from successful reproduction of farmed individuals. It is thus imperative to better understand the reproductive characteristics of farmed oysters, since they are directly under human control. We quantified the dry tissue mass (DTM), gametosomatic index (GSI), and reproductive cycle of farmed oysters at two sites in Bourgneuf Bay, France, in relation to environmental parameters using continuously-recording probes in 2005 and 2006. The GSI was developed for this study, based on the actual area occupied by gametes, rather than the area of the gonad previously used for... |
| Tipo: Text |
Palavras-chave: Atlantic Ocean; Crassostrea gigas; Oyster farming; Bivalve; Gametosomatic index; Biological invasion; Atresia; Oocytes; Turbidity; Temperature; Reproduction. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6871.pdf |
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| Registros recuperados: 23 | |
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