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| Registros recuperados: 34 | |
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| FUJlHIRA, Takuma; KINOSHITA, Mikio; SASAKI, Motoki; OHNISHI, Masao; ISHIKAWA, Hajime; OHSUMI, Seiji; FUKUI, Yutaka; 木下, 幹朗; 佐々木, 基樹; 大西, 正男. |
| The present study was conducted to clarify the difference in the color of the cytoplasm in immature follicular oocytes from prepubertal and adult minke whales. The four lipid contents (triglyceride，total cholesterol phospholipids and non-esterified fatty acids) in vitrified immature oocytes from prepubertal and adult minke whales，and also in fresh and vitrified immature porcine oocytes，were measured. The lipid contents in vitrified-warmed minke whale oocytes were similarly high compared with those in vitrified-warmedp orcine oocytes. In particular，the total cholesterol and phospholipid contents in the vitrified immature oocytes from prepubertal and adult minke whales were significantly( P<0.05) higher than those from prepubertal pigs. Furthermore，the... |
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Palavras-chave: Minke whale; Porcine; Oocytes; Lipid; Sexual maturity. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2731 |
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| CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M.. |
| Tipo: Artigo de periódico |
Palavras-chave: Activation; Embryos; Maturation; Aquaporin 3; Hsp70 Protein; Ionomycin; Heat Shock; Cleavage; Blastocyst; In vitro fertilization; Parthenogenesis; Apoptosis; Gene expression; Gametocytes; Oocytes. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1113387 |
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| CAMARGO, L. S. A.; COSTA, F. Q.; MUNK, M.; WOHLRES-VIANA, S.; SERAPIÃO, R. V.; CARVALHO, B. C.; CAMPOS JÚNIOR, P. H.; VIEIRA, A. C.; NOGUEIRA, L. A. G.; VIANA, J. H. M.. |
| Abstract This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus?oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleav‐age at 48 hpia. Heat shock had a negative impact (p... |
| Tipo: Artigo de periódico |
Palavras-chave: Activation; Embryos; Maturation; Aquaporin 3; Hsp70 Protein; Ionomycin; Heat Shock; Cleavage; Blastocyst; In vitro fertilization; Parthenogenesis; Apoptosis; Gene expression; Gametocytes; Oocytes. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118469 |
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| BRAGANÇA, G. M.; SOUZA-FABJAN, J. M. G.; BATISTA, R. I. T. P.; RIBEIRO, L. S.; BRAIR, V. L.; CÔRTES, L. R.; SOUZA, C. V.; TRAIRA, A. R.; FONSECA, J. F. da; MENCHACA, A.; BRANDÃO, F. Z.. |
| In attempt to develop a hormonal protocol more suitable to produce good quality oocytes for use in biotechnologies, an earlier study demonstrated that FSH applied in multiple decreasing doses produced better quality oocytes. However, different progestagens implants used during ovarian stimulation have not yet been tested. The aim of this study was to investigate the effect of different progestagens during ovarian stimulation on follicular population and oocyte morphological quality in Santa Inês ewes. In conclusion, the source of progestagens used during the ovarian stimulation protocol does not affect the follicular population, nor the oocyte quality. Exogenous progestagens may not be necessary when postsynchronization ovulation is confirmed by... |
| Tipo: Artigo de periódico |
Palavras-chave: Animal reproduction; Sheep; Ewes; Oocytes. |
| Ano: 2018 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1095105 |
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| Cruz,Maria Helena Coelho; Saraiva,Naiara Zoccal; Cruz,Jurandir Ferreira da; Oliveira,Clara Slade; Del Collado,Maite; Fernandes,Hugo; Castro,Fernanda Cavallari de; Garcia,Joaquim Mansano. |
| This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo development; Inner cell mass; In vitro fertilization; Oocytes. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300003 |
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| MENEZES, V. G.; MONTE, A. P. O.; BARBERINO, R. de S.; GOUVEIA, B. B.; LINS, T. L. B.; MACEDO, T. J. S. e; OLIVEIRA JÚNIOR, J. L. de; ARAÚJO, C. de A.; CAMPOS, F. S.; OLIVEIRA FILHO, E. F. de; BATISTA, A. M.; QUEIROZ, M. A. A.; ARAUJO, G. G. L. de; MATOS, M. H. T. de; WISCHRA , A.. |
| This study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were... |
| Tipo: Artigo de periódico |
Palavras-chave: Foliculogênese; Folículos primordiais; Oócito; Hormônios sexuais; Ovelha; Água Potável; Drinking water; Oocytes; Sex hormones; Sheep. |
| Ano: 2023 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781 |
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| SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; OLIVEIRA, J. C.; GARCIA, J. M.. |
| The developmental competence of enucleated oocytes is a key factor that determines the overall success of animal cloning. Enucleation is an invasive procedure in traditional nuclear transfer (NT). The objective of this work was to evaluate the effects of demecolcine, a microtubule-depolymerizing agent, on metaphase II (MII) bovine oocytes and to verify the capacity of embryonic development after NT using chemically assisted enucleation. In the first experiment, oocytes after 21 h of IVM were exposed for 2 h to several concentrations of demecolcine: 0 (control), 0.025, 0.05, 0.2, and 0.4 µg mL?1, and evaluated in relation to membrane protrusion formation. After the best concentration of demecolcine was determined, the nuclear and microtubular dynamics of... |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: BOVINO; Oocytes. |
| Ano: 2008 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/48309 |
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| RODRIGUES, S. A. D.; PONTELO, T. P.; KUSSANO, N. R.; KAWAMOTO, T. S.; LEME, L. O.; CAIXETA, F. M. C.; PFEIFER, L. F. M.; FRANCO, M. M.; DODE, M. A. N.. |
| We aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in... |
| Tipo: Artigo de periódico |
Palavras-chave: E2; Oócito; Maturation; F2a.; Bovinocultura; Reprodução Animal; Cultura In Vitro; Prostaglandina.; Maturação; Embriogénese; Ovulo; In vitro studies; Cattle; Animal reproduction; Embryogenesis; Oocytes; Prostaglandins.. |
| Ano: 2020 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1127907 |
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| Oliveira,Classius de; Santos,Lia Raquel de Souza. |
| This paper describes morphological characteristics of the ovarian germinative cells of the Scinax fuscovarius (Lutz, 1925). The ovary is organized in primordial germinative cells (oogonia) and follicular structures (ovarian follicle) - oocytes surrounded by follicular cells. Oogonia: their nests are peripherically localized, containing cells with large and oval nucleus. Oocytes I: basophilic cytoplasm; the spherical nucleus presents few nucleoli, one or two; the follicular cells (one tenuous layer) surround these previtellogenic cells. Oocytes II: the cell is larger and the cytoplasm becomes more basophilic; the nucleus presents few nucleoli and contains many chromosomes in the periphery (beginning the perinucleolar stage). Oocytes III: the cytoplasm... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oocytes; Oogonia; Ovary; Pigment; Vitellogenesis. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752004000400029 |
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| Pessoa,G.A; Navarro,R.B; Rauber,L.P; Brass,K.E; Silva,C.A.M; Rubin,M.I.B. |
| Temperature and rainfall were analyzed daily during six years to evaluate their influence on in vitro production of bovine embryos. Weekly replications (n=480) were performed on 14,778 ovaries collected at slaughterhouses. Cumulus oocyte complexes (n=19,180) were fertilized with a pool of Bos taurus taurus semen in one incubator with 5% CO2. Presumable zygotes were cultured in gasified plastic bags with 5% CO2, 5% O2, and 90% N2. In the first year, cleavage and embryo yield were 60.3% and 15.6%, respectively, being lower (P<0.05) than in the following years. Average cleavage rates were always lower in winter (P<0.0001), thus producing less embryos. Winter climatic conditions had a negative influence on in vitro production, when cleavage and embryo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cow; Oocytes; Embryo; Climate; In vitro prodution. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600013 |
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| Sanches,Eduardo Antônio; Caneppele,Danilo; Okawara,Renan Yoshiharu; Damasceno,Danielle Zanerato; Bombardelli,Robie Allan; Romagosa,Elizabeth. |
| Abstract The Steindachneridion parahybae is an endangered catfish from Brazil and strategies applied for gametes optimization are necessary. The aim of this study was to assess inseminating doses and water volume upon the fertilization, hatching rates and percentage of normal larvae in S. parahybae . Was used a randomized design in factorial scheme (4×4) with four inseminating doses: 1.0×104, 1.0×105, 1.0×106, 1.0×107spermatozoa oocyte-1 and four volumes of water: 1, 35, 65 and 95mL of water g-1 of oocytes. The combination of doses and volumes were performed in triplicates (n=48). Each incubator (1.5L of useful volume) with 1g of oocytes was considered as an experimental unit. Significant interaction between inseminating doses and volumes of water to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Artificial reproduction; Freshwater fish; Oocytes; Sperm; Surubim-do-Paraíba. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000100204 |
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| Max, Marilu Constantino; Andrade, Evelyn Rabelo de; Basso, Andrea Cristina; de Figueiredo, José Ricardo; Seneda, Marcelo Marcondes. |
| Objective: To disclose a new biotechnique, listing some of its aspects and addressing its main stages, isolation, cryopreservation and culture of preantral follicles. Data Sources: The information was obtained from CAB, MEDLINE, PUBMED, Science Direct and CAPES Portal. Data Synthesis: The preantral follicles store about 90% of the oocytes within the ovary. The biotechnique described is based on recovering immature oocytes retained inside preantral follicles before atresia takes place. The cryopreservation of these cells enables the creation of germoplasm banks, preserving the genetic material of women submitted to chemotherapy and/or radiotherapy, as well as that endangered animal species. Conclusions: Being a recent biotechnique, its application is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biotechonology; Reproductive techniques; Oocytes; Transplantation; Ovarianfollicles; Cryopreservation Biotecnologia; Técnicas reproductivas; Oocitos; Transplantación; Foliculo ovárico; Criopreservación biotecnologia; Técnicas reprodutivas; Oócitos; Transplante; Folículo ovariano; Criopreservação. |
| Ano: 2004 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3239 |
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| Navarrete Gómez,Patricia; Ormeño,David; Miranda,Antonio; Sánchez Gutiérrez,Raúl; Romero Mejía,Fernando; Parodi Rivera,Jorge. |
| Since the 1970s, There have been studies of the venom of Latrodectus sp. spiders, in particular the latrotoxin (LTX) of Latrodectus mactans. Many of the studies were aimed at understanding the action of the venom on the muscular system. Now accepted that LTX is able to generate a calcium-permeable membrane pore and modulate the release of synaptic vesicles that activate a receptor and induce cellular changes. Interestingly, when work began with venom obtained from the Latrodectus sp present in Chile, it generated clinical indications similar to the bite of this spider in another country, with some differences in intensity. The purpose of the first studies was to understand the systemic mechanisms of this venom, and other active compounds were studied for... |
| Tipo: Journal article |
Palavras-chave: Latrodectus venom; Oocytes; Neurons; Spermatozoa; Contraceptive. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300012 |
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| CASTRO, A. P.; SIMÕES, L. M. S.; BOTTINO, M. P.; PONTES, T. T.; RODRIGUES, S. de A. D.; CAIXETA, F. M. C.; QUINTAO, C. C. R.; CAMARGO, L. S. de A.; DODE, M. A. N.; SALES, J. N. de S.. |
| Tipo: Anais e Proceedings de eventos |
Palavras-chave: Intrafollicular; Immature; Injection; Oocytes. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085854 |
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| Registros recuperados: 34 | |
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