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Registros recuperados: 23
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Assisted reproductive techniques in small animals MV&Z
Lopes, Maria Denise.
The objective of this discussion is to bring information about some of the assisted reproductive techniques used in domestic animals, and the impact of this technology on reproduction of dogs and cats.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatozoa; Oocytes; Fertilization; Reproductive techniques espermatozóide; Oócito; Fertilização; Técnicas reprodutivas; Cão; Gato.
Ano: 2001 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3341
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Biologia reproductiva de la saraquita Ramnogaster arcuata (Jenyns, 1842) del estuario del Rio de la Plata. OceanDocs
Rodrigues, K.A..
The reproductive biology of Ramnogaster arcuata inhabiting waters of the Rio de la Plata estuary (Argentina - Uruguay) was studied by using macroscopic and histological analysis of ovaries. R. Arcuata specimens were collected from two research cruises of the National Institute of Fisheries Research and Development (INIDEP) carried out in June 1998 and June 1999. During these surveys, bottom salinity and temperature data were also registered. In addition, information about annual catches of this species in the Rio de la Plata, obtained from different INIDEP cruises performed during the last ten years, was analyzed. During winter, a prevalence of R. arcuata individuals was observed in the Rio de la Plata estuary. In summer, this species apparently moves...
Tipo: Theses and Dissertations Palavras-chave: Sexual reproduction; Ovaries; Fecundity; Spawning seasons; Histology; Fish eggs; Oocytes; Environmental conditions; Ovaries; Sexual reproduction; Fecundity; Oocytes; Environmental conditions; Http://aims.fao.org/aos/agrovoc/c_5456; Http://aims.fao.org/aos/agrovoc/c_26876; Http://aims.fao.org/aos/agrovoc/c_15725; Http://aims.fao.org/aos/agrovoc/c_29343; Http://aims.fao.org/aos/agrovoc/c_32980.
Ano: 2005 URL: http://hdl.handle.net/1834/1485
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Comparative Studies on lipid Analysis and Ultrastructure in Porcine and Southern Minke Whale (Balaenoptera Bonaerensis) Oocytes OAK
FUJlHIRA, Takuma; KINOSHITA, Mikio; SASAKI, Motoki; OHNISHI, Masao; ISHIKAWA, Hajime; OHSUMI, Seiji; FUKUI, Yutaka; 木下, 幹朗; 佐々木, 基樹; 大西, 正男.
The present study was conducted to clarify the difference in the color of the cytoplasm in immature follicular oocytes from prepubertal and adult minke whales. The four lipid contents (triglyceride,total cholesterol phospholipids and non-esterified fatty acids) in vitrified immature oocytes from prepubertal and adult minke whales,and also in fresh and vitrified immature porcine oocytes,were measured. The lipid contents in vitrified-warmed minke whale oocytes were similarly high compared with those in vitrified-warmedp orcine oocytes. In particular,the total cholesterol and phospholipid contents in the vitrified immature oocytes from prepubertal and adult minke whales were significantly( P<0.05) higher than those from prepubertal pigs. Furthermore,the...
Palavras-chave: Minke whale; Porcine; Oocytes; Lipid; Sexual maturity.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2731
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Donnees cytologiques, electrophysiologiques et biochimiques relatives au phenomene de fecondation chez le homard (Homarus gammarus ). Implication du processus d'activation sur le mecanisme d'attache des oeufs pondus ArchiMer
Goudeau, M; Lachaise, F; Kubisz, P; Goudeau, H.
Electrophysiological measurements and related cytological controls were done on immature and submature oocytes of Homarus gammarus . By chromatography (HPLC) and radio-immunoassays (RIA) it was established that submature oocytes displayed different ecdysteroids. Furthermore, the structure of the stalk (funiculus) for the laid eggs attachment was analyzed. it consisted of the two superimposed vitelline envelopes which were likely modified by the cortical reaction exudate during the fertilization process. In addition, the successive secretory phases, regarding to the embryonic envelopes were evidenced. Finally, the ecdysteroid titers of embryonic stages were measured.
Tipo: Text Palavras-chave: Homarus gammarus; Malacostraca; Biological attachment; Hormones; Electrophysiology; Biochemistry; Cytology; Eggs; Embryonic development; Cell membranes; Oocytes; Fecundity; Homard; Decapode; Crustacé; Funicule; Mues embryonnaires; Enveloppe de fécondation; Ecdystéroîdes; Potentiel membranaire; Ovocyte.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1190.pdf
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Effect of follicular fluid supplementation during in vitro maturation on total cell number in bovine blastocysts produced in vitro R. Bras. Zootec.
Cruz,Maria Helena Coelho; Saraiva,Naiara Zoccal; Cruz,Jurandir Ferreira da; Oliveira,Clara Slade; Del Collado,Maite; Fernandes,Hugo; Castro,Fernanda Cavallari de; Garcia,Joaquim Mansano.
This study evaluated the influence of follicular fluid (FF) added to the maturation medium on the quality of bovine embryos produced in vitro. In the first experiment, oocytes were matured in media containing five different FF concentrations with different maturation times and classified according to meiotic progression and migration of cortical granules. In the second experiment, oocytes matured in the same media were fertilized at three different maturation times; thereafter, cleavage and blastocyst rates were evaluated. In the third experiment, oocytes were matured in media containing three different FF concentrations at two different maturation times, and embryo quality, inferred by the ratio of inner cell mass and trophectoderm cells compared with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Embryo development; Inner cell mass; In vitro fertilization; Oocytes.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300003
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Effect of two ram sperm capacitating media on acrosome reaction and zona-free hamster oocyte penetration test Braz. J. Vet. Res. Anim. Sci.
FERRARI,Silvia; BARNABE,Valquíria Hyppolito; ZÜGE,Roberta Mara; ZOGNO,Maria Amélia.
An in vitro zona-free hamster oocyte penetration test was utilized in 24 trials in order to evaluate the capacitation media used for ram sperm. A pool of fresh semen was collected from three crossed breed rams. Two semen drops were washed by centrifugation and incubated in high ionic strength treatment (HIS) or in a defined medium with HEPES, on heat ewe serum and heparin. After the incubation to promote capacitation, simplified triple-stain technique was used to evaluate the spontaneous acrosome reaction of the capacitated sperm. Superovulation in 96 golden hamsters was induced by PMSG and hCG. The oocytes were treated with hyaluronidase and trypsin to remove, respectively, the cumulus cells and the zona pellucida. Oocytes and capacitated sperm were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Capacitation; Spermatozoa; Rams; Oocytes; Hamsters.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000300010
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Histological characterization of cellular types during Scinax fuscovarius oogenesis (Lutz) (Anura, Hylidae) Zoologia
Oliveira,Classius de; Santos,Lia Raquel de Souza.
This paper describes morphological characteristics of the ovarian germinative cells of the Scinax fuscovarius (Lutz, 1925). The ovary is organized in primordial germinative cells (oogonia) and follicular structures (ovarian follicle) - oocytes surrounded by follicular cells. Oogonia: their nests are peripherically localized, containing cells with large and oval nucleus. Oocytes I: basophilic cytoplasm; the spherical nucleus presents few nucleoli, one or two; the follicular cells (one tenuous layer) surround these previtellogenic cells. Oocytes II: the cell is larger and the cytoplasm becomes more basophilic; the nucleus presents few nucleoli and contains many chromosomes in the periphery (beginning the perinucleolar stage). Oocytes III: the cytoplasm...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oocytes; Oogonia; Ovary; Pigment; Vitellogenesis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752004000400029
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Influence of climatic conditions on in vitro production of bovine embryos Arq. Bras. Med. Vet. Zootec.
Pessoa,G.A; Navarro,R.B; Rauber,L.P; Brass,K.E; Silva,C.A.M; Rubin,M.I.B.
Temperature and rainfall were analyzed daily during six years to evaluate their influence on in vitro production of bovine embryos. Weekly replications (n=480) were performed on 14,778 ovaries collected at slaughterhouses. Cumulus oocyte complexes (n=19,180) were fertilized with a pool of Bos taurus taurus semen in one incubator with 5% CO2. Presumable zygotes were cultured in gasified plastic bags with 5% CO2, 5% O2, and 90% N2. In the first year, cleavage and embryo yield were 60.3% and 15.6%, respectively, being lower (P<0.05) than in the following years. Average cleavage rates were always lower in winter (P<0.0001), thus producing less embryos. Winter climatic conditions had a negative influence on in vitro production, when cleavage and embryo...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cow; Oocytes; Embryo; Climate; In vitro prodution.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000600013
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Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish Neotropical Ichthyology
Sanches,Eduardo Antônio; Caneppele,Danilo; Okawara,Renan Yoshiharu; Damasceno,Danielle Zanerato; Bombardelli,Robie Allan; Romagosa,Elizabeth.
Abstract The Steindachneridion parahybae is an endangered catfish from Brazil and strategies applied for gametes optimization are necessary. The aim of this study was to assess inseminating doses and water volume upon the fertilization, hatching rates and percentage of normal larvae in S. parahybae . Was used a randomized design in factorial scheme (4×4) with four inseminating doses: 1.0×104, 1.0×105, 1.0×106, 1.0×107spermatozoa oocyte-1 and four volumes of water: 1, 35, 65 and 95mL of water g-1 of oocytes. The combination of doses and volumes were performed in triplicates (n=48). Each incubator (1.5L of useful volume) with 1g of oocytes was considered as an experimental unit. Significant interaction between inseminating doses and volumes of water to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artificial reproduction; Freshwater fish; Oocytes; Sperm; Surubim-do-Paraíba.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000100204
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Main aspeets of the manipulation of ooeytes enelosed in preantral ovarian follieles MV&Z
Max, Marilu Constantino; Andrade, Evelyn Rabelo de; Basso, Andrea Cristina; de Figueiredo, José Ricardo; Seneda, Marcelo Marcondes.
Objective: To disclose a new biotechnique, listing some of its aspects and addressing its main stages, isolation, cryopreservation and culture of preantral follicles. Data Sources: The information was obtained from CAB, MEDLINE, PUBMED, Science Direct and CAPES Portal. Data Synthesis: The preantral follicles store about 90% of the oocytes within the ovary. The biotechnique described is based on recovering immature oocytes retained inside preantral follicles before atresia takes place. The cryopreservation of these cells enables the creation of germoplasm banks, preserving the genetic material of women submitted to chemotherapy and/or radiotherapy, as well as that endangered animal species. Conclusions: Being a recent biotechnique, its application is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechonology; Reproductive techniques; Oocytes; Transplantation; Ovarianfollicles; Cryopreservation Biotecnologia; Técnicas reproductivas; Oocitos; Transplantación; Foliculo ovárico; Criopreservación biotecnologia; Técnicas reprodutivas; Oócitos; Transplante; Folículo ovariano; Criopreservação.
Ano: 2004 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3239
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Molecular Characterization, Electrophysiological and Contraceptive Effect of Chilean Latrodectus Venom International Journal of Morphology
Navarrete Gómez,Patricia; Ormeño,David; Miranda,Antonio; Sánchez Gutiérrez,Raúl; Romero Mejía,Fernando; Parodi Rivera,Jorge.
Since the 1970s, There have been studies of the venom of Latrodectus sp. spiders, in particular the latrotoxin (LTX) of Latrodectus mactans. Many of the studies were aimed at understanding the action of the venom on the muscular system. Now accepted that LTX is able to generate a calcium-permeable membrane pore and modulate the release of synaptic vesicles that activate a receptor and induce cellular changes. Interestingly, when work began with venom obtained from the Latrodectus sp present in Chile, it generated clinical indications similar to the bite of this spider in another country, with some differences in intensity. The purpose of the first studies was to understand the systemic mechanisms of this venom, and other active compounds were studied for...
Tipo: Journal article Palavras-chave: Latrodectus venom; Oocytes; Neurons; Spermatozoa; Contraceptive.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000300012
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Ovarian histology and fecundity in the evaluation of the reproduction of the invasive species Serrasalmus marginatus (Characidae) on a neotropical floodplain Biological Sciences
Melo, Gabriele Sauthier Romano de; Santana, Herick Soares de; Tos, Claudenice Dei.
 The construction of the Itaipu Hydroelectric Power Plant in 1982 led to the formation of a reservoir, which, in turn, leveled the waters of the Paraná River by flooding the geographic barrier Salto de Sete Quedas. This allowed the piranha Serrasalmus marginatus to invade and colonize the upper Paraná River. This study aimed to: i) confirm, through light microscopy, the reproductive phases of S. marginatus females; ii) estimate fecundity and iii) evaluate the reproduction areas of the population. A total of 764 females were collected from nine sampling sites on the upper Paraná River floodplain. Microscopic analysis of the ovaries showed the following phases: early developing subphase, developing phase, spawning capable phase, actively spawning subphase,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.05.02.00-1 Indeterminate fecundity; Type of spawning; Oogenesis; Oocytes; Piranha. Ecologia de Ecossistemas.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33021
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Preventing Effect of Vitamin E on Oocytes Apoptosis in Morphine-treated Mice International Journal of Morphology
Asadi,Ebrahim; Shabani,Ronak; Ghafari,Soraya; Golalipour,Mohammad Jafar.
Several studies have shown that Morphine Sulfate affects on fertility, embryogenesis and consequent pregnancy loss and ultrastructural alterations of oocytes in animal model. This study was done to determine the effect of morphine sulfate on oocytes apoptosis and preventive role of daily supplementation of Vitamin E on oocytes apoptosis in morphine sulfate -treated mice. Twenty-four NMARI female mice were randomly allocated into four experimental groups. For 15 days, control group received saline (0.2 ml/day by subcutaneous injection), group I Vitamin E (60 mg/kg/day orally), group II Morphine Sulfate (10 mg/kg/day by subcutaneous injection) and group III Morphine Sulfate with Vitamin E (60 mg/kg/day orally). Then, animals were superovulated with PSMG (10...
Tipo: Journal article Palavras-chave: Oocytes; Morphine sulfate; Vitamin E; Apoptosis; TUNEL assay; Mice.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200029
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PRODUCTION OF OVOGONIA BY GERMINATIVE EPITHELIUM OF THE OVARY AT SOME MAMMALIA AND BIRDS Ciência Rural
Sincai,Mariana; Marcu,Adrian.
The studies were effected on ovaries from sows, cows, bitches, dous and hens. Our investigations revealed the generation of cellular rows of germinative epithelium into the cortex not only during the embrionary period but also in the adult ovary. The histological investigations revealed an intense proliferative process of the germinative epithelium of adult ovary which generated groups of ovogonia under the lamina basalis. From ovogonia developed oocytes and then ovarian follicles. The cause for the proliferation of ovary germinative epithelium could be: the multiple folliculary dehiscences, the premature degeneration of ovarian follicles and formation of the corpus luteum, phenomena that reduce continually the number of embrionary ovogonia.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovary; Epithelium; Mammalian; Hens; Proliferation; Ovogonia; Oocytes; Ovarian follicles.
Ano: 1993 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000300018
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Relations entre le contenu lipidique des ovocytes de Pecten maximus (Mollusque, bivalve) et les performances des larves en élevage ArchiMer
Le Pennec, Marcel; Gueguen,.
The lipid content of spawned oocytes of Pecten maximus was studied by image analysis of semi -thin histological sections and a lipid index was defined. The female gametes were obtained by thermic stimulations of mature adults scallops from different populations after out of season conditioning or sampled in situ during the spawning season. After fertilization, the larval development was followed. The results reveal a large variability in lipid content of normal oocytes. The lipid content can be regarded as homogeneous inside a spawning but the heterogeneity is greater for wild scallops than for conditioned ones. A strong relationship appears between the lipid index and the hatching success, and the number of abnormal larvae after two days rearing. But no...
Tipo: Text Palavras-chave: Mollusques; Bivalves; Pecten maximus; Ovocytes; Lipides; Elevages larvaires; Mollusc; Bivalve; Pecten maximus; Oocytes; Lipids; Larval development.
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00022/13345/10379.pdf
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Reproduction and growth of the deep-sea brittlestar ophiura-ljungmani (lyman) ArchiMer
Tyler, Pa; Gage, Jd.
Reproductive biology and population dynamics of Ophiura Ijungmani were studied by measuring disk diameters and by examining oocyte size-frequencies and fecundity from a sequential seasonal series of large samples from a 2900 m deep Permanent Station in the Rockall Trough (Northeast Atlantic). Egg size (max. 90 !lm) and fecundity (up to 57000 eggs per individual) suggests indirect planktotrophic development, while a clear annual cycle in gametogenesis, with probably synchronous spawning in JanuaryFebruary, confirms a marked seasonality that is also seen as an annual, summertime set of postlarvae on to the bottom. Analysis of size structure of the population indicates annually discrete year classes with a maximum longevitv of around 10 years. lndividuals...
Tipo: Text Palavras-chave: Ovocytes; Fécondité; Structure de la répartition des classes de tailles; Recrutement; Capacité de survie; Oocytes; Fecundity; Population structure; Recruitment; Survivorship.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/00323/43424/43087.pdf
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Reproduction of the Brazilian snapper, Lutjanus alexandrei Moura &amp; Lindeman, 2007 (Perciformes: Lutjanidae), off the northern coast of Pernambuco, Brazil Neotropical Ichthyology
Fernandes,Cezar A. F.; Oliveira,Paulo G. V. de; Travassos,Paulo E. P.; Hazin,Fábio H. V..
Reproductive aspects of the Brazilian snapper Lutjanus alexandrei, were characterized, including a description of the development of oocytes and spermatogenic cells, size at first sexual maturity, and fecundity. A total of 540 fish were analyzed with 250 having their gonads sectioned to allow microscopic evaluation. Six maturity stages were identified for females and males: immature, maturing, mature, spawning, spawned, and resting. Fish standard length (SL) varied from 13.0 to 28.3 cm and sex ratio was 1.6 males: 1.0 females. Monthly distributions of mean Gonadosomatic Index (GSI) and maturity stages suggest that spawning occurs mainly in a protracted period, during the warmer months, from November to March. The size of first sexual maturity was estimated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Multiple spawning; Oocytes; Sexual maturity; Spermatogenic cells.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300012
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Short-term preservation of Pecari tajacu ovarian preantral follicles using phosphate buffered saline (PBS) or powdered coconut water (ACP(r)) media Arq. Bras. Med. Vet. Zootec.
Lima,G.L.; Santos,E.A.A.; Lima,L.F.; Luz,V.B.; Rodrigues,A.P.R.; Silva,A.R..
We compare protocols for the short-term preservation of collared peccarie's ovarian preantral follicles (PFs) by using phosphate buffered saline- (PBS) or powdered coconut water- (ACP(r)) based medium. For morphology analysis each pair of ovaries collected from six females was divided into nine fragments. One fragment was destined for morphology analysis (histology and transmission electron microscopy - TEM), constituting the control group and the other fragments were placed in tubes with PBS or ACP(r), packed in 5 L Styrofoam boxes, stored for 4h, 12h, 24h, and 36h, and then analyzed. For viability analysis a pair of ovaries from two additional females was divided into nine fragments; one fragment was immediately destined for viability analysis (Trypan...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Collared peccaries; Preantral follicles; Coconut water; Ovary; Oocytes.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601623
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Structural and Ultrastructural Aspects of Folliculogenesis in Didelphis albiventris, the South-American Opossum International Journal of Morphology
Cesario,Maria Dalva; Matheus,Selma M. Michelin.
The ovarian histology, the structural and the ultrastructural characteristics of the folliculogenesis in Didelphis albiventris were described in detail. Recent studies suggest that methatherian mammals have unusual reproductive cycle but there are few informations regarding the marsupials reproductive life. Despite of the opossum folliculogenesis pattern resembles methatherian and eutherian pattern in many aspects, the analysis shows some peculiar features of the oocyte structure and ultrastructure that make available new data on the reproductive biology of marsupials.
Tipo: Journal article Palavras-chave: Folliculogenesis; Oocytes; Opossum; Marsupial.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000100019
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Temperature and seston quantity and quality effects on field reproduction of farmed oysters, Crassostrea gigas, in Bourgneuf Bay, France ArchiMer
Dutertre, Mickael; Beninger, Peter G.; Barille, Laurent; Papin, Mathias; Rosa, Philippe; Barille, Anne-laure; Haure, Joel.
The proliferation of the voluntarily-introduced cupped oyster, Crassostrea gigas, has attained the proportions of species invasion in many intertidal habitats in Europe, presumably resulting from successful reproduction of farmed individuals. It is thus imperative to better understand the reproductive characteristics of farmed oysters, since they are directly under human control. We quantified the dry tissue mass (DTM), gametosomatic index (GSI), and reproductive cycle of farmed oysters at two sites in Bourgneuf Bay, France, in relation to environmental parameters using continuously-recording probes in 2005 and 2006. The GSI was developed for this study, based on the actual area occupied by gametes, rather than the area of the gonad previously used for...
Tipo: Text Palavras-chave: Atlantic Ocean; Crassostrea gigas; Oyster farming; Bivalve; Gametosomatic index; Biological invasion; Atresia; Oocytes; Turbidity; Temperature; Reproduction.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6871.pdf
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