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| Thekisoe, Oriel M. M.; Honda, T; Fujita, H; Battsetseg, B; Hatta, T; Fujisaki, Kozo; Sugimoto, Chihiro; Inoue, Noboru. |
| Common arthropod vectors for trypanosomes are flies, fleas and bugs. This study reports on an unknown trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks, hereby, referred to as Trypanosoma KG1 isolate. The parasite has been successfully cultured in vitro with L929 or HEK 293T cell line as feeder cells. This trypanosome cannot survive in vitro without feeder cells. Following experimental infections of ticks, the trypomastigote-like and the epimastigote-like forms of this trypanosome could be detected by Giemsa-stained smears of the midgut and salivary glands of Ornithodoros moubata ticks which were made to feed on a culturing medium containing Trypanosoma KG1 isolate through an artificial membrane. Trypanosoma KG1 isolate... |
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Palavras-chave: Trypanosoma KG1 isolate; Haemaphysalis hystricis; Ornithodoros moubata. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1047 |
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| Ruheta, Martin R.; Inoue, Noboru; Fujisaki, Kozo. |
| Ornithodoros moubata was fed artificially through a parafilm membrane with two types of artificial meals. The first type was composed of bovine and horse red blood cells suspended in phosphate buffered saline or fetal bovine serum (FBS). The second type was composed of FBS alone. Engorgement and post engorgement survival rates of the ticks after feeding on the two types of meals were compared and analysed statistically. FBS was found to be superior to red blood cell meals. Eventually two batches of FBS from different manufacturers were used to feed the ticks through artificial membrane. Engorgement and post engorgement survival rates were again compared and analysed statistically. There was no significant difference between the two lots. Finally O. moubata... |
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Palavras-chave: Ornithodoros moubata; Fetal bovine serum; Bovine red blood cells; Horse red blood cells; Artificial membrane feeding. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/152 |
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