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Registros recuperados: 18
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Analysis of microbial diversity in Shenqu with different fermentation times by PCR-DGGE BJM
Liu,Tengfei; Jia,Tianzhu; Chen,Jiangning; Liu,Xiaoyu; Zhao,Minjie; Liu,Pengpeng.
Abstract Shenqu is a fermented product that is widely used in traditional Chinese medicine (TCM) to treat indigestion; however, the microbial strains in the fermentation process are still unknown. The aim of this study was to investigate microbial diversity in Shenqu using different fermentation time periods. DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) profiles indicated that a strain of Pediococcus acidilactici (band 9) is the predominant bacteria during fermentation and that the predominant fungi were uncultured Rhizopus, Aspergillus oryzae, and Rhizopus oryzae. In addition, pathogenic bacteria, such as Enterobacter cloacae, Klebsiella oxytoca, Erwinia billingiae, and Pantoea vagan were detected in Shenqu. DGGE analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial diversity; PCR-DGGE; Shenqu; Molecular cloning.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200246
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Application of molecular fingerprinting for analysis of a PAH-contaminated soil microbiota growing in the presence of complex PAHs - DOI: 10.4025/actascibiolsci.v32i1.7575 Biological Sciences
Silva, Ísis Serrano; UNICAMP; Menezes, Cristiano Ragagnin de; UNICAMP; Faria, Andréia Fonseca de; UNICAMP; Costa, Josemar Gurgel da; UFRN; Pepper, Susan; The University of Melbourne; Britz, Margaret; The University of Melbourne; Durrant, Lucia Regina; UNICAMP.
Polycyclic aromatic hydrocarbons (PAHs) constitute a group of priority pollutants which are present at high concentrations in the soils of many industrial contaminated sites. Pollution by these compounds may stimulate growth of organisms able to live in these environments causing changes in the structure of the microbial community due to some cooperative process of metabolization of toxic compounds. A long-term PAH-contaminated soil was stored for several years and used to analyze the native microbiota regarding their ability to grow on pyrene, benzo[a]pyrene, as well as in mixtures of LMW- and HMW-PAHs. Molecular profiles of the microbial community was assessed by PCR-DGGE of 16S rRNA gene, and the number of bands observed in DGGE analyses was interpreted...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polycyclic aromatic hydrocarbons (PAHs); Biodegradation; PCR-DGGE; Microbial community Polycyclic aromatic hydrocarbons (PAHs); Biodegradation; PCR-DGGE; Microbial community.
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7575
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Arbuscular mycorrhizal fungal communities in the rhizosphere of a continuous cropping soybean system at the seedling stage BJM
Cui,Jiaqi; Bai,Li; Liu,Xiaorui; Jie,Weiguang; Cai,Baiyan.
Abstract Arbuscular mycorrhizae (AM) fungi play a crucial role in the growth of soybean; however, the planting system employed is thought to have an effect on AM fungal communities in the rhizosphere. This study was performed to explore the influence of continuous soybean cropping on the diversity of Arbuscular mycorrhizal (AM) fungi, and to identify the dominant AM fungus during the seedling stage. Three soybean cultivars were planted under two and three years continuous cropping, respectively. The diversity of AM fungi in the rhizosphere soil at the seedling stage was subsequently analyzed using polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). The results showed that an increase in cropping years improved the colonization...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soybean; Continuous cropping; Arbuscular mycorrhizae; PCR-DGGE; Genetic diversity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200240
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Bacteria diversity and microbial biomass in forest, pasture and fallow soils in the southwestern Amazon basin Rev. Bras. Ciênc. Solo
Cenciani,Karina; Lambais,Marcio Rodrigues; Cerri,Carlos Clemente; Azevedo,Lucas Carvalho Basílio de; Feigl,Brigitte Josefine.
It is well-known that Amazon tropical forest soils contain high microbial biodiversity. However, anthropogenic actions of slash and burn, mainly for pasture establishment, induce profound changes in the well-balanced biogeochemical cycles. After a few years the grass yield usually declines, the pasture is abandoned and is transformed into a secondary vegetation called "capoeira" or fallow. The aim of this study was to examine how the clearing of Amazon rainforest for pasture affects: (1) the diversity of the Bacteria domain evaluated by Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis (PCR-DGGE), (2) microbial biomass and some soil chemical properties (pH, moisture, P, K, Ca, Mg, Al, H + Al, and BS), and (3) the influence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Land use changes; PCR-DGGE; Bacterial diversity; Microbial biomass; Principal component analysis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832009000400015
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Comparison of DNA extraction protocols for microbial communities from soil treated with biochar. Repositório Alice
LEITE, D. C. A.; BALIEIRO, F. C.; C. A. PIRES; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. C.; PEIXOTO, R. S..
2014
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Biochar; Extração de DNA; PCR-DGGE; Comunidades microbianas; Índice de pureza do DNA.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1007986
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Comparison of DNA extraction protocols for microbial communities from soil treated with biochar BJM
Leite,D.C.A.; Balieiro,F.C.; Pires,C.A.; Madari,B.E.; Rosado,A.S.; Coutinho,H.L.C.; Peixoto,R.S..
Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercial DNA extraction...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Biochar; DNA extraction; PCR-DGGE; Microbial communities; DNA purity indices.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100023
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Deslocamento miscível de um efluente de indústria de explosivo em colunas de solo Bragantia
Garcia,William Vilar; Alcântara,Marco Aurélio Kondracki de; Camargo,Otávio Antonio de; Izário Filho,Hélcio José; Andreote,Fernando Dini.
Este estudo teve como objetivo avaliar alguns impactos decorrentes do deslocamento miscível de efluente de nitração de uma indústria de explosivos aplicado em colunas de um Latossolo Amarelo, horizonte B (LA-B), submetido aos tratamentos: adição de carbonatos (BASE), ácidos (ACID), fosfato (FOSF), carbonatos e fosfato (BASE-FOSF) e ácidos e fosfatos (ACID-FOSF). A recuperação de nitrogênio em relação ao total aplicado varia entre 10,1 (ACID) e 65,5% (BASE). Há correlação significativa entre as curvas de transposição de N obtidas experimentalmente e as simuladas pelo aplicativo STANMOD para a maioria das colunas (p<0,001). A exceção ocorreu para ACID-FOSF (p=0,202). Não há correlação entre carga eletrostática líquida (CEL) e as variáveis de ajuste do...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Deslocamento miscível; Nitrogênio; Curvas de transposição; PCR-DGGE.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052012000100015
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Diversidade bacteriana da rizosfera de genótipos de milho contrastantes na eficiência de uso de fósforo. Repositório Alice
OLIVEIRA, C. A. de; MARRIEL, I. E.; GOMES, E. A.; LANA, U. G. de P.; SCOTTI, M. R.; ALVES, V. M. C..
Resumo - O objetivo deste trabalho foi avaliar a diversidade funcional e genética de bactérias associadas à rizosfera de genótipos de milho contrastantes quanto à eficiência de uso de fósforo, por meio do teste de fontes de carbono no sistema EcoPlate e da eletroforese em gel de gradiente desnaturante (DGGE) dos fragmentos amplificados dos genes 16S ribossomais (rDNA) das bactérias. Foram coletadas amostras de solo da rizosfera de linhagens e híbridos contrastantes quanto à eficiência de uso de fósforo, cultivados em Latossolo Vermelho-Escuro fase cerrado, com baixo e alto teor de P. Bactérias da rizosfera de híbridos e linhagens eficientes, sob estresse de P, analisadas pelo sistema EcoPlate, tenderam a se agrupar conforme a análise de componentes...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Deficiência de fósforo; PCR-DGGE; Sistema EcoPlate; Zea mays; Microbial ecology.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/662024
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Diversidade bacteriana da rizosfera de genótipos de milho contrastantes na eficiência de uso de fósforo PAB
Oliveira,Christiane Abreu de; Marriel,Ivanildo Evódio; Gomes,Eliane Aparecida; Lana,Ubiraci Gomes de Paula; Scotti,Maria Rita; Alves,Vera Maria Carvalho.
O objetivo deste trabalho foi avaliar a diversidade funcional e genética de bactérias associadas à rizosfera de genótipos de milho contrastantes quanto à eficiência de uso de fósforo, por meio do teste de fontes de carbono no sistema EcoPlate e da eletroforese em gel de gradiente desnaturante (DGGE) dos fragmentos amplificados dos genes 16S ribossomais (rDNA) das bactérias. Foram coletadas amostras de solo da rizosfera de linhagens e híbridos contrastantes quanto à eficiência de uso de fósforo, cultivados em Latossolo Vermelho-Escuro fase cerrado, com baixo e alto teor de P. Bactérias da rizosfera de híbridos e linhagens eficientes, sob estresse de P, analisadas pelo sistema EcoPlate, tenderam a se agrupar conforme a análise de componentes principais, o...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Deficiência de fósforo; Ecologia microbiana; PCR-DGGE; Sistema EcoPlate.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009001100015
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Early Changes in Soil Metabolic Diversity and Bacterial Community Structure in Sugarcane under Two Harvest Management Systems Rev. Bras. Ciênc. Solo
Azevedo,Lucas Carvalho Basilio; Morais,Marcio; Lambais,Marcio Rodrigues.
Preharvest burning is widely used in Brazil for sugarcane cropping. However, due to environmental restrictions, harvest without burning is becoming the predominant option. Consequently, changes in the microbial community are expected from crop residue accumulation on the soil surface, as well as alterations in soil metabolic diversity as of the first harvest. Because biological properties respond quickly and can be used to monitor environmental changes, we evaluated soil metabolic diversity and bacterial community structure after the first harvest under sugarcane management without burning compared to management with preharvest burning. Soil samples were collected under three sugarcane varieties (SP813250, SP801842 and RB72454) and two harvest management...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 16S rRNA gene; Harvest management; Metabolic profile; Microbial activity; PCR-DGGE.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832015000300701
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Extraction of bulk DNA from Thar Desert soils for optimization of PCR-DGGE based microbial community analysis Electron. J. Biotechnol.
Kumar Gothwal,Raj; Kumar Nigam,Vinod; Mohan,M. Krishna; Sasmal,Dinakar; Ghosh,Purnendu.
A reliable method for characterizing microbial communities on the basis of their differences in the 16S ribosomal RNA (rRNA) gene sequences in the hot arid zone sandy soils has been optimized. A desert plant (Calligonum polygonoides) was chosen to provide the rhizospheric soil samples, collected from three different agro-ecological locations. Total community DNA was efficiently extracted at small-scale level using direct lysis with hot sodium dodecyl sulphate (SDS), glass bead beating and finally subjecting the sandy soil to liquid nitrogen freeze-thaw cycles. To amplify V3 region of bacterial 16S rRNA gene, universal conserved primers were used. Second round polymerase chain reaction (PCR) was attempted to increase product concentration and to minimize...
Tipo: Journal article Palavras-chave: Arid soils; Microbial community; PCR-DGGE; Rhizosphere; Soil DNA extraction.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300007
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Identification and assessment of kefir yeast potential for sugar/ethanol-resistance BJM
Miguel,M.G.C.P.; Cardoso,P.G.; Magalhães-Guedes,K.T.; Schwan,R.F..
Biochemical and molecular analysis was used for identification of different kefir yeasts species from Brazil, Canada and the United States of America. The sugar/ethanol-resistant activity of the yeasts was evaluated. Saccharomyces cerevisiae and Kluyveromyces marxianus had the highest growth rates, suggesting biotechnological applications possible for these strains.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kefir; Sugar/ethanol-resistant yeasts; Yeasts diversity; Rep-PCR; PCR-DGGE.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100016
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Inventory and monitoring of wine microbial consortia Inra
Renouf, V.; Claisse, O.; Lonvaud-Funel, A..
The evolution of the wine microbial ecosystem is generally restricted to Saccharomyces cerevisiae and Oenococcus oeni, which are the two main agents in the transformation of grape must into wine by acting during alcoholic and malolactic fermentation, respectively. But others species like the yeast Brettanomyces bruxellensis and certain ropy strains of Pediococcus parvulus can spoil the wine. The aim of this study was to address the composition of the system more precisely, identifying other components. The advantages of the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) approach to wine microbial ecology studies are illustrated by bacteria and yeast species identification and their monitoring at each stage of wine production....
Tipo: Journal Article Palavras-chave: SACCHAROMYCES CEREVISIAE; ASCOMYCOTINA; ENDOMYTALES; SACCHAROMYCES; OENOCOCCUS OENI; BRETTANOMYCES BRUXELLENSIS; PEDIOCOCCUS PARVULUS; BACTERIA; STREPTOCOCCACEAE; PEDIOCOCCUS; PCR-DGGE; INVENTAIRE; IDENTIFICATION.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20077b2366db&uri=/notices/prodinra1/2010/09/
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Is the nitrification a redundant process in arid regions?: activity, abundance and diversity of nitrifier microorganisms RChHN
NOE,LAURA; ABRIL,ADRIANA.
We tested if the microbial functional redundancy concept (theory relating changes in ecosystem functioning by species loss) apply for changes in the ammonium-oxidizing community (nitrifier bacteria) due to: a) grazing disturbance, b) seasonal variations (dry season vs. wet season), c) habitat type (soil vs. organic residues), and d) ecological conditions (two eco-regions), in an arid region of central-western of Argentina. We determined: a) abundance of culturable nitrifier bacteria, b) nitrification activity and efficiency, and c) nitrifier community genetic structure by PCR-DGGE (richness and similarity index). Grazing did not change the nitrifier abundance, activity and richness, while seasonally and eco-region effects were scarce. Contrarily, the...
Tipo: Journal article Palavras-chave: Arid Chaco; Monte desert; PCR-DGGE; Similarity index; Species replacement.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2013000300009
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Microbial diversity in an Oxisol under no-tillage and conventional tillage in southern Brazil Rev. Ciênc. Agron.
Babujia,Letícia Carlos; Silva,Adriana Pereira; Nogueira,Marco Antonio; Hungria,Mariangela.
The no-tillage (NT) system of soil management is recognized as more sustainable than conventional tillage (CT), with an important role played by soil microorganisms. The objective of this study was to estimate differences in soil microbial diversity under NT and CT at different soil depths. For that, bacterial (16S rDNA) and fungal (18S rDNA) communities were evaluated by denaturing gradient gel electrophoresis (DGGE) in a 20-year field experiment established on an Oxisol in southern Brazil on which soybean has been grown in the summer and wheat in the winter. Soil samples were collected at the depths of 0-5, 5-10, 10-20 and 20-30 cm, and submitted to DGGE analyses. The results revealed lower similarity (28%) between bacterial communities in the NT and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Fungal diversity; PCR-DGGE; Soil tillage.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000500001
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Microbial dynamics during azo dye degradation in a UASB reactor supplied with yeast extract BJM
Silva,S.Q.; Silva,D.C.; Lanna,M.C.S.; Baeta,B.E.L.; Aquino,S.F..
The present work aimed to investigate the microbial dynamics during the anaerobic treatment of the azo dye blue HRFL in bench scale upflow anaerobic sludge bed (UASB) reactor operated at ambient temperature. Sludge samples were collected under distinct operational phases, when the reactor were stable (low variation of color removal), to assess the effect of glucose and yeast extract as source of carbon and redox mediators, respectively. Reactors performance was evaluated based on COD (chemical oxygen demand) and color removal. The microbial dynamics were investigated by PCR-DGGE (Polimerase Chain Reaction - Denaturing Gradient of Gel Electrophoresis) technique by comparing the 16S rDNA profiles among samples. The results suggest that the composition of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azo dye; UASB reactor; PCR-DGGE; Wastewater treatment; Microbial profile.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400005
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Molecular and cultural analysis of seasonal actinomycetes in soils from Artemisia tridentata habitat Phyton
Gonzalez-Franco,AC; Robles-Hernandez,L; Nuñez-Barrios,A; Strap,JL; Crawford,DL.
In order to understand the temporal dynamics of actinomycete communities of the rhizosphere of the desert plant Artemisia tridentata (sagebrush), two complementary methods were used. They were: (1) 16S rDNA-based PCR coupled with denaturing gradient gel electrophoresis (DGGE), and (2) an agar plate enumeration methodology in which three different media were used to quantify total bacteria, actinomycetes, and fungi. The objective of this research were: (1) to obtain a comprehensive picture of the structure of actinomycete populations, and (2) their dynamics in the rhizosphere of young and old sagebrush plants during two distinct seasons. PCR-DGGE analysis showed that actinomycete groups were less diverse in rhizosphere soils collected in winter than in bulk...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinomycete populations; Seasonal actinomycetes; PCR-DGGE; Rhizosphere soil; Artemisia tridentata; Desert plants.
Ano: 2009 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572009000200001
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Temporal dynamics of microbial communities in the rhizosphere of two genetically modified (GM) maize hybrids in tropical agrosystems. Repositório Alice
COTTA, S. R.; DIAS, A. C. F.; MARRIEL, I. E.; GOMES, E. A.; ELSAS, J. D. van; SELDIN, L..
The use of genetically modified (GM) plants still raises concerns about their environmental impact. The present study aimed to evaluate the possible effects of GM maize, in comparison to the parental line, on the structure and abundance of microbial communities in the rhizosphere. Moreover, the effect of soil type was addressed. For this purpose, the bacterial and fungal communities associated with the rhizosphere of GM plants were compared by culture-independent methodologies to the near-isogenic parental line. Two different soils and three stages of plant development in two different periods of the year were included. As evidenced by principal components analysis (PCA) of the PCR-DGGE profiles of evaluated community, clear differences occurred in these...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: PCR-DGGE; Milho; Zea mays; Rizosfera; Planta transgênica.
Ano: 2013 URL: http://www.alice.cnptia.embrapa.br/handle/doc/950568
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