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Le Bourhis, A.G.; Saunier, K.; Dore, J.; Carlier, J.P.; Chamba, J.F.; Popoff, M.R.; Tholozan, J.L.. |
A nested-PCR temporal temperature gradient gel electrophoresis (TTGE) approach was developed for the detection of bacteria belonging to phylogenetic cluster I of the genus Clostridium (the largest clostridial group, which represents 25% of the currently cultured clostridial species) in cheese suspected of late blowing. Primers were designed based on the 16S rRNA gene sequence, and the specificity was confirmed in PCRs performed with DNAs from cluster I and non-cluster I species as the templates. TTGE profiles of the PCR products, comprising the V5-V6 region of the 16S rRNA gene, allowed us to distinguish the majority of cluster I species. PCR-TTGE was applied to analyze commercial cheeses with defects. All cheeses gave a signal after nested PCR, and on the... |
Tipo: Journal Article |
Palavras-chave: CHEESE; PCR-TTGE; CLASSIFICATION; RIBOSOMAL DNA. |
Ano: 2005 |
URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD200894b4c2a1&uri=/notices/prodinra1/2008/06/ |
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Mondeguer, Florence; Lepinay, Alexandra; Capiaux, Hervé; Turpin, Vincent; Baron, Regis; Hess, Philipp; Lebeau, Thierry; Boug,. |
The marine diatom Haslea ostrearia [1] produces a water-soluble blue-pigment named marennine [2] of economic interest. But the lack of knowledge of the ecological conditions, under which this microalga develops in its natural ecosystem, more especially bacteria H. ostrearia interactions, prevents any optimization of its culture in well-controlled conditions. The structure of the bacterial community was analyzed by PCR-TTGE before and after the isolation of H. ostrearia cells recovered from 4 localities, to distinguish the relative part of the biotope and the biocenose and eventually to describe the temporal dynamic of the structure of the bacterial community at two time-scales. The differences in genetic fingerprints, more especially high between two H.... |
Tipo: Text |
Palavras-chave: Haslea ostrearia; Marine diatom; Co-culture microalgae; Bacterial community; PCR-TTGE; High Resolution Mass Spectrometry; Untargeted metabolomics. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00342/45367/44862.pdf |
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