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| Xiong,XP; Song,Q; Han,CC; Gan,W; He,F; Wei,SH; Liu,HH; Xu,HY. |
| ABSTRACT To identify what makes insulin have an activating or inhibiting role in gluconeogenesis in goose hepatocytes and whether insulin regulates PEPCK and G6Pase through the PI3k/Akt/mTOR pathway or not, goose primary hepatocytes were isolated and cultured in vitro. After 12h cultured in serum-free medium, hepatocytes were incubated for 24 h in the medium with no addition (control) or with the addition of50, 100, and 150 nM of insulin, 1000 nM NVP-BEZ235, or co-addition of 150nM insulin and 1000nM NVP-BEZ235. Glucose concentration and PEPCK and G6Pase expression were determined. The results showed that PEPCK and G6Pase mRNA levels and activities were up regulated in the 50, 100, and 150nM insulin treatments, while glucose concentration was not... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: G6Pase; Insulin; PEPCK; PI3k/Akt/mTOR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300395 |
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| González-Santos,Rosalinda; Delgado-Alvarado,Adriana; Zavaleta-Mancera,H. Araceli; Herrera-Cabrera,B. Edgar. |
| Las citocininas disminuyen su concentración durante la senescencia foliar pero la aspersión de la citocinina bencilaminopurina (BAP) retrasa este proceso, lo cual puede tener aplicaciones en el control de la senescencia de pastos forrajeros, ornamentales de follaje y hortalizas verdes. El objetivo del presente trabajo fue investigar el efecto de la aplicación de BAP en el metabolismo primario durante la senescencia foliar de Triticum aestivum L. cv. Temporalera mediante el estudio del contenido de pigmentos fotosintéticos, proteínas solubles, Rubisco, carbohidratos, actividad enzimática de fosfoenolpiruvato carboxilasa (PEPC) y fosfoenolpiruvato carboxicinasa (PEPCK), componentes importantes del metabolismo primario vegetal. Plántulas de 21 d después de la... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum aestivum L.; Citocininas; Hexosas; Fosfoenolpiruvato carboxicinasa; PEPCK; Fosfoenolpiruvato carboxilasa; PEPC. |
| Ano: 2009 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952009000400005 |
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| Tang,B; Yang,C; Hu,S; Sun,W; Pan,Z; Li,L; Wang,J. |
| ABSTRACT Over-accumulation of triglycerides (TGs) in goose hepatocytes leads to the formation of fatty acid liver. Phosphoenolpyruvate carboxylase kinase 1 (PEPCK) is regarded as the rate-limiting enzyme for gluconeogenesis, and there is evidence that PEPCK is involved in regulating hepatic glucolipid metabolism. Hence, we proposed that PEPCK may have a role in goose hepatic steatosis. To test our hypothesis, the present study was conducted to firstly determine the sequence characteristics of goose PEPCK and then to explore its role in overfeeding-induced fatty liver. Our results showed that goose PEPCK encodes a 622-amino-acids protein that contains highly conserved oxaloacetate-binding domain, kinase-1 and kinase-2 motifs. PEPCK had higher mRNA levels in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goose; PEPCK; Sequence characteristics; Hepatic steatosis; Primary hepatocytes. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000400302 |
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