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Registros recuperados: 6
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Genetics of resistance to transgenic Bacillus thuringiensis poplars in the poplar leaf beetle, Chrysomela tremulae F. (Coleoptera: Chrysomelidae) Inra
Augustin, S.; Courtin, C.; Rejasse, A.; Lorme, P.; Genissel, A.; Bourguet, D..
The area under genetically engineered plants producing Bacillus thuringiensis (Bt) toxins is steadily increasing. This increase has magnified the risk of alleles conferring resistance to these toxins being selected in natural populations of target insect pests. The speed at which this selection is likely to occur depends on the genetic characteristics of Bt resistance. We selected a strain of the beetle Chrysomela tremulae Fabricius on a transgenic Bt poplar clone Populus tremula L. x Populus tremuloides Michx producing high levels of B.thuringiensis Cry3Aa toxin. This strain was derived from an isofemale line that generated some F-2 offspring that actively fed on this Bt poplar clone. The resistance ratio of the strain was >6,400. Susceptibility had...
Tipo: Journal Article Palavras-chave: PEUPLIER TRANSGENIQUE; RESISTANCE AUX FACTEURS NUISIBLES; ALLELE DE RESISTANCE; BT RESISTANCE; TRANSFORMATION GENETIQUE; EXPRESSION DES GENES COLEOPTERA; CHRYSOMELIDAE; CHRYSOMELA TREMULAE FABRICIUS.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007dbf4c9f9&uri=/notices/prodinra1/2007/08/
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High tolerance against Chrysomela tremulae of transgenic poplar plants expressing a synthetic cry3Aa gene from Bacillus thuringiensis ssp tenebrionis Inra
Génissel, A.; Leplé, J.C.; Millet, N.; Augustin, S.; Jouanin, L.; Pilate, G..
Hybrid poplars (Populus tremula ×Populus tremuloides) have been genetically engineered viaAgrobacterium tumefaciens, to express a syntheticcry3Aa gene derived from the native Bacillusthuringiensis subsp. tenebrionis cry3Aa gene.The presence and the expression of the transgene have been verified in fourtransgenic poplar lines, using Southern, northern and western analyses. Thetransgenic poplar's toxicity towards the phytophagous beetleChrysomela tremulae (Coleoptera, Chrysomelidae) has beenassessed on six month-old greenhouse-grown selected plants in laboratoryconditions. Laboratory experiments consisted of feeding tests of fresh detachedleaves on C. tremulae at all developmental stages. Ourresults indicate that the transgenic poplar leaves, expressing a...
Tipo: Journal Article Palavras-chave: PEUPLIER TRANSGENIQUE; RESISTANCE AUX INSECTES NUISIBLES; EXPRESSION DES GENES; TOXICITE DES FEUILLES BACILLUS THURINGIENSIS; CHRYSOMELA TREMULAE.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007fc73fa80&uri=/notices/prodinra1/2007/06/
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Modified lignification in the cell walls of cad depressed poplars Inra
Yoshinaga, A.; Wada, M.; Fujita, M.; Chabbert, B.; Pilate, G..
Ultraviolet microscopic spectrophotometry was used to investigate lignificationin the secondary cell walls of wood fibers and vessel elementsfrom either wild type or transgenic poplars with depressed activity ofcinnamyl alcohol dehydrogenase (CAD). A distinct shoulder at 330 nmwas characteristic of the transgenic poplar. Measurements performedafter 1) a mild alkali treatment or 2) a reduction with sodium borohydrideindicated that this shoulder merely resulted from the occurrence of conjugatedcarbonyl groups in the lignin polymer rather than alkali-solublecell wall bound phenolic aldehydes. UV absorbance ratios (A330 /A280)measured in the center of secondary walls in the differentiating xylemclearly showed that the structural changes observed in the lignin...
Tipo: Journal Article Palavras-chave: PEUPLIER TRANSGENIQUE; ALCOOL CINNAMYLIQUE DESHYDROGENASE; SPECTROPHOTOMETRIE TRANSGENIC POPLAR; CELL WALL; WOOD FIBER; CINNAMYL ALCOHOL DESHYDROGENASE; CAD.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20097e76961a&uri=/notices/prodinra1/2009/09/
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Quantitative 31P NMR spectroscopy of lignins from transgenic poplars Inra
Akim, L. G.; Argyropoulos, D. S.; Jouanin, L.; Leplé, J.C.; Pilate, G.; Pollet, B.; Lapierre, C..
Milled wood lignins isolated from genetically modified poplar trees were studied by quantitative 31P NMR spectroscopy, in combination with thioacidolysis and mild alkaline hydrolysis. The genetictransformation of the trees included down regulation of CAD and COMT enzyme activities.These analyses confirmed that moderate CAD down-regulation does not substantially alter ligninstructure. In contrast, severe CAD deficiency alters the lignin structure more profoundly by decreasingthe syringyl/guaiacyl ratio and increasing the degree of condensation of the lignin. Themost severe alterations were observed in the lignins from the COMT-transformed lines.
Tipo: Journal Article Palavras-chave: PEUPLIER TRANSGENIQUE; RMN; RESONANCE MAGNETIQUE NUCLEAIRE; THIOACIDOLYSE; HYDROLYSE ALCALINE TRANSGENIC POPLAR; NMR; NUCLEAR MAGNETIC RESONANCE; THIOACIDOLYSIS; ALKALINE HYDROLYSIS.
Ano: 2001 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20071e4afa41&uri=/notices/prodinra1/2008/12/
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Stability of transgene expression in poplar: A model forest tree species Inra
Hawkins, S.; Leplé, J.C.; Cornu, D.; Jouanin, L.; Pilate, G..
Notre étude a pour but d'évaluer la stabilité de l'expression d'un transgène chez un peuplier hybride (Populus tremula x P. alba) transformé avec le gène reporter uidA sous le contrôle soit du promoteur 35S, soit du promoteur du gène cad2 de l'eucalyptus (EuCAD) à spécificité vasculaire. L'expression du transgène a été suivie quantitativement par fluorimétrie et qualitativement par histochimie à partir de matériel collecté in vitro, en serre ou en champ sur quelques centaines d'arbres, issus de 44 lignées transgéniques. Tandis qu'il existe d'importantes variations dans les niveaux d'expression du transgène selon les lignées, les arbres d'une même lignée, l'organe analysé ou la date de prélèvement, l'expression du transgène s'est révélée être stable sur une...
Tipo: Journal Article Palavras-chave: PEUPLIER TRANSGENIQUE; ESPECE MODELE; STABILITE; EXPRESSION DES GENES; TRANSGENE.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007245d82c2&uri=/notices/prodinra1/2009/12/
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Transgenic poplar and the poplar leaf beetle: state-of-the-art on the risk of evolution of insect resistance Inra
Augustin, S.; Carré, G.; Courtin, C.; Dubois, V.; Genissel, A.; Lorme, P.; Wenes, A.L.; Rejasse, A.; Pilate, G.; Leplé, J.C.; Bourguet, D..
Tipo: Meeting Paper Palavras-chave: PEUPLIER TRANSGENIQUE; RESISTANCE AUX RAVAGEURS; ALLELE DE RESISTANCE; COUT DE LA RESISTANCE TRANSGENIC POPLAR; HARDWOOD; PEST RESISTANCE; RESISTANCE ALLELE; RESISTANCE COST; GMO.
Ano: 2004 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2007a544caaa&uri=/notices/prodinra1/2009/08/
Registros recuperados: 6
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