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Registros recuperados: 36
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Associated risk factors and pulsed field gel electrophoresis of nasal isolates of Staphylococcus aureus from medical students in a tertiary hospital in Lagos, Nigeria BJID
Adesida,Solayide A.; Abioye,Olusegun A.; Bamiro,Babajide S.; Brai,Bartholomew I.C.; Smith,Stella I.; Amisu,Kehinde O.; Ehichioya,Deborah U.; Ogunsola,Folasade T.; Coker,Akitoye O..
Staphylococcus aureus infections are growing problems worldwide with important implications in hospitals. The organism is normally present in the nasal vestibule of about 35% of apparently healthy individuals and its carriage varies between different ethnic and age groups. Staphylococcal nasal carriage among health workers is particularly important to establish new clones and track origin of infections during outbreak situations. To determine the carriage rate and compare the pulsed field gel patterns of the strains, nasal swabs were collected from 185 medical students in a teaching hospital in Lagos, Nigeria. Isolates of S. aureus were tested for heamolysin production, methicillin sensitivity and Pulsed Field Gel Electrophoresis (PFGE) was performed. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; PFGE; Nasal isolates; Carriage rate.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100016
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Caracterização molecular de Staphylococcus aureus isolados de amostras de leite de vacas do município de Prudente de Morais, Minas Gerais. Repositório Alice
RIBEIRO, J. B.; RIBEIRO, G. R.; ALVES, H. T.; SILVA, Y. P.; REIS, L. da S.; FELIX, W. M. B.; QUEIROZ, L. da S.; GUIMARAES, A. S.; LADEIRA, C. V. G.; CERQUEIRA, M. M. O. P..
2016
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Mastite bovina; Produção de leite; PFGE; Qualidade do leite.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1062439
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Carbapenem-resistant Pseudomonas aeruginosa outbreak in an intensive care unit of a teaching hospital BJID
Gales,Ana C.; Torres,Patrícia L.; Vilarinho,Dominique S. O.; Melo,Renata S.; Silva,Claudinéia F. L.; Cereda,Rosângela F..
The genetic similarity of carbapenem-resistant Pseudomonas aeruginosa strains isolated in the Hospital Universitário São Francisco, Bragança Paulista, São Paulo, Brasil, was evaluated by pulsed field gel electrophoresis (PFGE). A unique clone was detected among 5 of 7 isolates, suggesting that cross-contamination might have played a role in the spread of carbapenem-resistant P. aeruginosa strains. Interestingly, a similar PFGE pattern was encountered in a P. aeruginosa strain isolated from Hospital São Paulo that was used as a PFGE control.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; PFGE; Carbapenem.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000400001
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Characterization of Listeria monocytogenes isolated from a fresh mixed sausage processing line in Pelotas-RS by PFGE BJM
von Laer,Ana Eucares; Lima,Andréia Saldanha de; Trindade,Paula dos Santos; Andriguetto,Cristiano; Destro,Maria Teresa; Silva,Wladimir Padilha da.
Listeria monocytogenes is a bacterium capable to adhere to the surfaces of equipment and utensils and subsequently form biofilms. It can to persist in the food processing environmental for extended periods of time being able to contaminate the final product. The aim of this study was to trace the contamination route of L. monocytogenes on a fresh mixed sausage processing line, from raw material to the final product. The isolates obtained were characterized by serotyping and molecular typing by pulsed-field gel electrophoresis (PFGE) using the restriction enzymes ApaI and AscI. L. monocytogenes was detected in 25% of the samples. The samples of raw material were not contaminated, however, the microorganism was detected in 21% of the environmental samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; PFGE; Serotyping; Persistent strains.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300021
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Characterization of Vibrio cholerae isolates from 1976 to 2013 in Shandong Province, China BJM
Lü,Hui; Yuan,Yuqi; Sun,Na; Bi,Zhenwang; Guan,Bing; Shao,Kun; Wang,Tongzhan; Bi,Zhenqiang.
Abstract Cholera continues to be a serious public health issue in developing countries. We analyzed the epidemiological data of cholera from 1976 to 2013 in Shandong Province, an eastern coastal area of China. A total of 250 Vibrio cholerae isolates were selected for PCR analysis of virulence genes and pulsed-field gel electrophoresis (PFGE). The analysis of the virulence genes showed that the positive rates for tcpA and tcpI were the highest among strains from the southwest region, which had the highest incidence rate of cholera. Low positive rates for tcpA, tcpI and ctxAB among isolates from after 2000 may be an influencing factor contributing to the contemporary decline in cholera incidence rates. Spatiotemporal serotype shifts (Ogawa, Inaba, Ogawa,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vibrio cholerae; Epidemiology; PFGE; Virulence genes.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100173
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Chronic infection of cystic fibrosis patient airways by a single clone of Burkholderia cepacia: replacement of non-mucoid to mucoid morphotype BJM
Carvalho,Ana Paula D'Alincourt; Ventura,Grasiella Maria de Carvalho; Folescu,Tânia Wrobel; D'Amoed,Renato Farme; Higa,Laurinda; Teixeira,Lucia Martins; Merquior,Vânia Lúcia Carreira; Marques,Elizabeth de Andrade.
Mucoid Burkholderia cepacia morphotype emerged within a nine year follow-up of a cystic fibrosis patient. Clinical data suggested a linkage between the mucoid phenotype isolation and the deterioration of the patient's condition. Despite of the phenotypic variation, molecular typing showed that the patient was chronically infected with B. cepacia complex isolates belonging to a same genetic clone.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cystic Fibrosis; Burkholderia cepacia complex; PFGE; RAPD-PCR.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500014
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Description of Methicillin-resistant Staphylococcus pseudintermedius from canine pyoderma in Minas Gerais state, Brazil Arq. Bras. Med. Vet. Zootec.
Bourguignon,E.; Viçosa,G.N.; Corsini,C.M.M.; Moreira,M.A.S.; Nero,L.A.; Conceição,L.G..
Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is of worldwide concern in veterinary medicine. The identification of resistant strains is necessary for proper treatment and the prevention of its propagation among animals. This study aimed to identify S. pseudintermedius isolated from canine pyoderma and evaluate their resistance profiles. Lesions from 25 dogs with pyoderma were sampled. Bacterial isolates were subjected to phenotypic and genotypic analysis for identification of the causative agent. S. pseudintermedius isolates were subjected to SmaI macrorestriction analysis and PFGE for genetic grouping, and PCR to identify the presence of the mecA gene. Their resistance profiles against 12 antimicrobials were also assessed. According to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus pseudintermedius; Canine pyoderma; MecA; PFGE; Antimicrobial resistance.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200299
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DNA Profiles of Salmonella Spp. Isolated from Chicken Products and From Broiler and Human Feces Rev. Bras. Ciênc. Avic.
Tejada,TS; Silva,CSJ; Lopes,NA; Silva,DT; Agostinetto,A; Silva,EF; Menezes,DB; Timm,CD.
ABSTRACT Salmonella species have been isolated from various kinds of food and are accountable for outbreaks of foodborne diseases in humans. This study aimed at identifying the similarities between the DNA profiles of Salmonella isolated from chicken feces, chicken products, and human feces in southern Brazil. Six hundred samples were collected (200 from chicken products, 200 from broiler chicken feces, and 200 from human feces) and tested for the presence of Salmonella. Isolates proven to be Salmonella compatible by biochemical and serological tests were tested by the Polymerase Chain Reaction. Their DNA profiles were then analyzed by PFGE and rep-PCR. Salmonella was isolated from 16 out of 600 analyzed samples, with Schwarzengrund serotype presenting the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mbandaka; Schwarzengrund; Panama; PFGE; Rep-PCR.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400693
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Epidemiological and molecular characterization of community and hospital acquired Staphylococcus aureus strains prevailing in Shenyang, Northeastern China BJID
Sun,Dan Dan; Ma,Xiao Xue; Hu,Jian; Tian,Yuan; Pang,Long; Shang,Hong; Cui,Long Zhu.
In order to obtain adequate information for the treatment of methicillin resistant Staphylococcus aureus (MRSA) infections, it is crucial to identify trends in epidemiological and antimicrobial resistance patterns of local S. aureus strains. Community and hospital acquired S. aureus isolates (n = 202) were characterized using staphylococcal cassette chromosome mec (SCCmec) typing, pulse field gel electrophoresis (PFGE) analysis, spa typing and minimal inhibitory concentration (MIC) determination. The prevalence of the Panton-Valentine leukocidine (pvl) and several antibiotic resistance genes among the isolates were also detected by PCR. All of the S. aureus isolates were susceptible to vancomycin, daptomycin and linezolid. Three hospital isolates were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CA-and HA-MRSA; SCCmec; PFGE; Spa; Pvl gene.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600010
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Epidemiological Survey of Listeria monocytogenes in a gravlax salmon processing line BJM
Cruz,C.D.; Silvestre,F.A.; Kinoshita,E.M.; Landgraf,M.; Franco,B.D.G.M.; Destro,M.T..
Listeria monocytogenes is a cause of concern to food industries, mainly for those producing ready-to-eat (RTE) products. This microorganism can survive processing steps such as curing and cold smoking and is capable of growing under refrigeration temperatures. Its presence in RTE fish products with extended shelf life may be a risk to the susceptible population. One example of such a product is gravlax salmon; a refrigerated fish product not exposed to listericidal processes and was the subject of this study. In order to evaluate the incidence and dissemination of L. monocytogenes 415 samples were collected at different steps of a gravlax salmon processing line in São Paulo state, Brazil. L. monocytogenes was confirmed in salmon samples (41%), food contact...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Listeria monocytogenes; Gravlax salmon; PFGE.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200032
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Evaluation of the Susceptibility profiles, genetic similarity and presence of qnr gene in Escherichia coli resistant to ciprofloxacin isolated in Brazilian hospitals BJID
Pereira,Andrea S.; Andrade,Soraya S.; Monteiro,Jussimara; Sader,Helio S.; Pignatari,Antonio C.C.; Gales,Ana C..
Increasing quinolone resistance has been reported worldwide, mainly among clinical isolates of Escherichia coli. The objectives of this study were to determine the susceptibility profile, the genetic relatedness, and the prevalence of the qnr gene among ciprofloxacin-resistant Escherichia coli isolated from distinct Brazilian hospitals. A total of 144 ciprofloxacin-resistant Escherichia coli were isolated from 17 Brazilian hospitals between January/2002 and June/2003. The antimicrobial susceptibility testing was performed by microdilution according to NCCLS. The presence of the qnr gene was initially screened by colony blotting, and then confirmed by PCR followed by DNA sequencing. Ninety-five urinary ciprofloxacin-resistant Escherichia coli were further...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Quinolone resistance; Escherichia coli; PFGE; Qnr.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100011
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Genetic diversity and antimicrobial resistance in Staphylococcus aureus and coagulase?negative Staphylococcus isolates from bovine mastitis in Minas Gerais, Brazil. Repositório Alice
DORNELES, E. M. S.; FONSECA, M. D. A. M.; ABREU, J. A. P.; LAGE, A. P.; BRITO, M. A. V. P.; PEREIRA, C. R.; BRANDAO, H. de M.; GUIMARÃES, A. S.; HEINEMANN, M. B..
Abstract The aims of this study were to determine the antimicrobial susceptibility profile and genetic diversity of Staphylococcus spp. isolated from dairy cows in Minas Gerais, Brazil, and to assess the relationship among the isolates? susceptibility profiles and pulsed‐field gel electrophoresis (PFGE) genotypes. Seventy‐nine isolates were used, including S. aureus (n = 71) and coagulase‐negative staphylococci (CoNS) (n = 8). Susceptibility to 12 antimicrobial agents was performed. All Staphylococcus spp. were subjected to PFGE. Staphylococcus aureus and CoNS isolates exhibited full susceptibility only to cephalothin. The greatest percentages of resistance among Staphylococcus spp. were observed to penicillins, folate pathway...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Antimicrobial resistance; Coagulase-negative staphylococci; Methicillin-resistant S aureus; PFGE; Staphylococci.
Ano: 2019 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118368
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Genetic diversity of Xanthomonas axonopodis pv. citri based on plasmid profile and pulsed field gel electrophoresis Genet. Mol. Biol.
Carvalho,Flávia Maria de Souza; Caramori,Lázara Pereira Campos; Leite Júnior,Rui Pereira.
Xanthomonas axonopodis pv. citri strains that cause disease in citrus were investigated by pulsed field and plasmid profile analysis. For the first method, genomic DNA was digested by the rare-cutting enzymes Xba I and Vsp I. The strains evaluated were collected in seven different States of Brazil and in Argentina, Bolivia, Paraguay and Uruguay. Genetic variability was found among strains of X. axonopodis pv. citri from different geographical areas Argentina, Bolivia and Uruguay, with similarities varying from 0.62 to 0.83. However, the strains collected in Brazil, despite being from different States, have shown a genetic similarity ranging from 0.83 to 1.00. Cluster analysis showed a relationship between genomic similarity and geographical origin of the...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Genetic variability; Bacterium; Citrus; PFGE.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300020
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Genetic relatedness among clinical strains of Stenotrophomonas maltophilia in tertiary care hospital settings in São Paulo State, Brazil BJM
Almeida,Margarete Teresa Gottardo; Rubio,Fernando Gôngora; Garcia,Doroti Oliveira; Pavarino-Bertelli,Érika Cristina; Rossit,Andrea Regina Baptista; Bando,Silvia Yano; Silbert,Suzane; Goloni-Bertollo,Eny Maria; Soares,Márcia Maria Costa Nunes; Martinez,Marina Baquerizo.
Stenotrophomonas maltophilia is a Gram-negative bacillus, which is becoming widely recognized as an important nosocomial pathogen. The main objective of this study was to evaluate the genetic relatedness, by random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of 86 clinical isolates of S. maltophilia (colonization 22, infection 64) obtained from 79 hospitalized patients, from different geographic regions of São Paulo State. The genotypic analysis performed by RAPD and PFGE was used in 24 isolates for genetic identity confirmation. The results were congruent between the two methods but it was not possible to link genetic profiles with the studied variables, clinical state and geographic area, probably due to the great...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stenotrophomonas maltophilia; RAPD; PFGE.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200017
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Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients BJID
Pretto,Luana; de-Paris,Fernanda; Machado,Alice Beatriz Mombach Pinheiro; Martins,Andreza Francisco; Barth,Afonso Luís.
Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients. The aim of this work was to perform pulsed-field gel electrophoresis-based molecular typing of B. cenocepacia isolates to evaluate the epidemiology of this species at our hospital. A total of 28 isolates from 23 cystic fibrosis patients were analyzed. Initially, we compared isolates obtained from the same patient at different periods of time. We then compared the pulsed-field gel electrophoresis profiles of 15 IIIA isolates, and in a third analysis, evaluated the genetic profile of 8 IIIB isolates from...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Burkholderia cenocepacia; Cystic fibrosis; PFGE; Genetic similarity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000100014
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Genome analysis of thirteen Colombian clostridial strains by pulsed field gel electrophoresis Electron. J. Biotechnol.
Quilaguy Ayure,Diana Milena; Suárez Moreno,Zulma Rocío; Aristizábal Gutierrez,Fabio Ancizar; Bernal Morales,Jose Mauricio; Montoya Castaño,Dolly.
Pulsed field gel electrophoresis was used for estimating the size of the genome and evaluating the presence of megaplasmids in 13 native Colombian solventogenic Clostridium strains. DNA preparation and purification were optimised for obtaining differentiated restriction fragments in electrophoresis. Genomic DNA was digested with ApaI, Eco52I, SmaI and XhoI enzymes. Estimated genome size for native strains ranged from 4.0 to 4.2 mega base pairs. Larger sized plasmids were detected and the presence of genes related to megaplasmid pSOL1 was determined by polymerase chain reaction. adc gene region amplification suggested that genes related to solventogenesis in native strains may be located in an extra-chromosomal element. Determining genome size provides...
Tipo: Journal article Palavras-chave: Clostridium; Genome size; PFGE; PSOL1.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500009
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Healthcare-associated vancomycin resistant Enterococcus faecium infections in the Mansoura University Hospitals intensive care units, Egypt BJM
Moemen,Dalia; Tawfeek,Doaa; Badawy,Wafaa.
Vancomycin resistant Enterococcus faecium (VREF) ia an emerging and challenging nosocomial pathogen. This study aimed to determine the prevalence, risk factors and clonal relationships between different VREF isolates in the intensive care units (ICUs) of the university hospitals in our geographic location. This prospective study was conducted from July, 2012 until September, 2013 on 781 patients who were admitted to the ICUs of the Mansoura University Hospitals (MUHs), and fulfilled the healthcare-associated infection (HAI) criteria. Susceptibility testing was determined using the disk diffusion method. The clonal relationships were evaluated with pulsed field gel electrophoresis (PFGE). Out of 52 E. faecium isolates, 12 (23.1%) were vancomycin resistant....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus faecium; ICU; Vancomycin; Risk factors; PFGE.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300777
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Identification, antimicrobial resistance and genotypic characterization of Enterococcus spp. isolated in Porto Alegre, Brazil BJM
Bender,Eduardo André; Freitas,Ana Lúcia Peixoto de; Reiter,Keli Cristine; Lutz,Larissa; Barth,Afonso Luís.
In the past two decades the members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. In the present study, we evaluated the antimicrobial resistance and genotypic characteristics of 203 Enterococcus spp. recovered from different clinical sources from two hospitals in Porto Alegre, Rio Grande do Sul, Brazil. The species were identified by conventional biochemical tests and by an automated system. The genetic diversity of E. faecalis presenting high-level aminoglycoside resistance (HLAR) was assessed by pulsed-field gel electrophoresis of chromosomal DNA after SmaI digestion. The E. faecalis was the most frequent specie (93.6%), followed by E. faecium (4.4%). The antimicrobial resistance profile was: 2.5% to ampicillin,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus; Resistance; HLAR; PFGE.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300035
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Imipenem-resistant Acinetobacter baumannii carrying the ISAba1-bla OXA-23, 51 and ISAba1-bla ADC-7 genes in Monteria, Colombia BJM
Martínez,Pedro; Mattar,Salim.
The purpose of this study was to identify the genes coding for resistance to ceftazidime and imipenem and describe the molecular epidemiology of A. baumannii strains isolated from a clinical center in Colombia. Twenty isolates of imipenem-resistant A. baumannii from an equal number of patients with nosocomial infections were obtained. Primers were used to amplify genes bla IMP, bla VIM, bla OXA-23, bla OXA-24, bla OXA-58, bla OXA-51 and bla ADC-7. To detect insertion sequences ISAba1/bla OXA-23, ISAba1/bla OXA-51 and ISAba1/bla ADC-7, mapping by PCR using combinations of reverse primers ISAba1 and reverse primers of bla OXA-23, bla OXA-51 and bla ADC-7 were used. The amplification products were purified and cloned into PCR 2.1-TOPO vector and transformed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nosocomial pathogens; Antimicrobial resistance; PFGE.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400006
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Molecular characterization of the first leptospires isolated from goats in Brazil BJM
Lilenbaum,Walter; Kremer,Frederico; Ristow,Paula; Dellagostin,Odir; Bourhy,Pascale; Hartskeerl,Rudy; Vasconcellos,Silvio.
Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospira; Serogrouping; Sequencing; PFGE; Rrs.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050
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