SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Bianchini,A.A.C.; Petroni,T.F.; Fedatto,P.F.; Bianchini,R.R.; Venancio,E.J.; Itano,E.N.; Ono,M.A..

The dimorphic fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, a human granulomatous disease. Recently the first case of natural disease in dogs was reported. The complement system is an important effector component of humoral immunity against infectious agents. Therefore, the aim of this study was to evaluate the activation of the dog alternative complement pathway by P. brasiliensis. Initially, the ability of erythrocytes of guinea pig, rabbit, sheep, chicken and swine to activate the dog alternative pathway was evaluated. The guinea pig erythrocytes showed the greatest capacity to activate dog alternative pathway. The alternative (AH50) hemolytic activity was evaluated in 27 serum samples from healthy dogs and the...

An unusual case of paracoccidioidomycosis in Brazil

Palavras-chave: Paracoccidioidomycosis; Dog; Complement.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200005

Macêdo,Danielle Patrícia Cerqueira; Neves,Rejane Pereira; Magalhães,Oliane Maria Correia; Lacerda Filho,Armando Marsden.

This paper reports an unusual case of paracoccidioidomycosis with ulcerations in the head and nose in a Brazilian man. For diagnosis, direct microscopic examination of ulcer secretion and skin samples treated with potassium hydroxide was performed. The isolation of Paracoccidioides brasiliensis occurred at 28ºC and 37ºC on Sabouraud agar with chloranphenicol. Diagnosis was established by the presence of isolated and multiple budding yeast-like cells and typical fungus growth of P. brasiliensis.

Biochemical responses in mice experimentally infected with Paracoccidioides brasiliensis and treated with Canova

Palavras-chave: Paracoccidioidomycosis; Paracoccidioides brasiliensis; Unusual head lesion.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100017

BABT

Takahachi,Gisele; Maluf,Márcia Luzia Ferrarezi; Svidzinski,Terezinha Inez Estivalet; Akimoto-Günther,Luciene Setsuko; Hübler,Márcia Rosangela Neves de Oliveira; Bersani-Amado,Ciomar Aparecida; Cuman,Roberto Kenji Nakamura.

The objective of this work was to evaluate biochemical parameters in Paracoccidioides brasiliensis infected mice and the effect of Canova medicine on these parameters. Mice infected with the isolate Paracoccidioides brasiliensis Pb18 and treated with Canova for 17 weeks were used. The biochemical parameters analysed were the levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and amylase, and the concentration of total proteins, albumin and globulins. The results suggested that the animals that were treated with Canova had less functional alterations in their internal organs.

Central Nervous System Paracoccidioidomycosis: an overview

Palavras-chave: Paracoccidioidomycosis; Biochemical profile; Canova; Paracoccidioides brasiliensis.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700006

Almeida,Sérgio Monteiro de.

Paracoccidioidomycosis (PCM) is an infectious disease, endemic to subtropical areas of Central and South America, caused by the dimorphic fungus Paracoccidioides brasiliensis. It is a chronic disease, mostly affecting adult males, with a mean patient age of 44 years. Central nervous system involvement (CNS PCM) has been found in 13% of the patients with systemic disease. We reviewed the clinical presentation, diagnosis techniques and treatments for CNS PCM.

Natural antibodies in paracoccidioidomycosis

Palavras-chave: Paracoccidioidomycosis; Neuroparacoccidioidomycosis; Cerebrospinal fluid; Paracoccidioides brasiliensis.

Ano: 2005

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000200002

Unterkircher,Carmelinda S.; Leão,Mariella V.P.; Blotta,Maria Heloisa S.L.; Camargo,Zoilo Pires de.

Recent attention has been focused on the natural antibodies as a component of natural immunity and as integral part of the idiotypic network. However, their functional role in different infections has rarely been studied. This work was undertaken to investigate the presence of natural antibodies in paracoccidioidomycosis (PCM). In addition, we analyzed anti-P. brasiliensis antibodies and their distribution in IgG subclasses in order to acquire better knowledge about the humoral immune response in this mycosis. Our findings show that the natural antibody response is not very much increased in PCM when compared with other parasite infections and this response is restricted to a few specificities, suggesting that P. brasiliensis moderately triggers CD5+ B...

Nuclear medicine in tropical diseases

Palavras-chave: Natural antibodies; Paracoccidioides brasiliensis; Paracoccidioidomycosis.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100009

BABT

Braga,Francisco José Hossri Nogueira.

Tropical diseases affect more people in the world than any other kind of disease, but scintigraphic data on that matter are not so frequent in the literature. Since the geographic regions where such diseases occur are normally very poor, scintillation cameras may not be available. We present a resumed summary of part of what has been done on this subject to-date. Leprosy affects circa 12 million people worldwide and has already been studied by means of the following scintigraphic exams: Gallium-67, 99mTc-MDP or HMDP, 99mTc-colloid or Dextran, 99mTc-DTPA and 99mTc-WBC. Paracoccidiodomycosis is a deep mycosis and such cases may be evaluated by means of Gallium-67, bone scintigraphy, lymphoscintigraphy, hepato-billiary, bone marrow and liver / spleen...

Paracoccidioidomycosis: description of two cases autochthonous in Ceará

Palavras-chave: Nuclear medicine; Scintigraphy; Tropical diseases; Leprosy; Paracoccidioidomycosis; Blastomycosis; Mucormycosis; Tuberculosis; Neurocysticercosis; Cutaneous and mucous leishmaniasis; Chagas disease.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000500002

Façanha,Mônica Cardoso; Souza,Anastácio de Queiroz; Café,Vaulice Sales; Wanke,Bodo.

Paracoccidioidomycosis is endemic in most countries of Latin America. Brazil has the greatest number of cases, with no autochthonous case registered in the state of Ceará. The cases of two patients that had never gone outside the State of Ceará and lived at least some years in the municipality of Palmácia.

Tipo: Info:eu-repo/semantics/report

Palavras-chave: Paracoccidioidomycosis; Ceará; Systemic mycosis.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100017

Protein-losing enteropathy in paracoccidioidomycosis identified by scintigraphy: report of three cases

Berni,Aline Dayara de Mendonça; Elias Júnior,Erivaldo; Arão Filho,Adalberto; Paniago,Anamaria Mello Miranda.

Paracoccidioidomycosis (PCM) or South American Blastomycosis is a systemic fungal infection caused by a dimorphic fungus, Paracoccidioides brasiliensis. It represents the most important systemic mycosis in South America and with higher prevalence among male inhabitants of the rural area. PCM usually affects the lungs, and rarely the intestines. The authors report three cases of PCM, treated at the University Hospital of Universidade Federal de Mato Grosso do Sul whose scintigraphy with technetium-99 labeled human albumin revead intestinal protein loss

Tipo: Info:eu-repo/semantics/report

Palavras-chave: Radionuclide imaging; Paracoccidioidomycosis; Intestinal diseases; Technetium Tc99m aggregated albumin.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500020

Recombinant 60-kDa heat shock protein from Paracoccidioides brasiliensis: is it a good antigen for serological diagnosis of paracoccidioidomycosis?

BJMBR

Peron,G.; Fernandes,F.F.; Landgraf,T.N.; Martinez,R.; Panunto-Castelo,A..

Paracoccidioides brasiliensis and P. lutzii are fungi that cause paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in South America. For serological diagnosis, although 43-kDa glycoprotein (gp43) is regarded as highly specific for PCM, the occurrence of false negative reactions in sera from patients infected with P. lutzii suggests that preparation with only one antigen is not recommended. Heat shock proteins are feasible alternatives as a second antigen because they are often highly immunogenic. In this study, we evaluated the usefulness of recombinant 60-kDa heat shock protein from P. brasiliensis (rPbHsp60) for the serological diagnosis of PCM. Using western blotting assay, we observed that 77.3% of the sera from PCM patients were...

Retrospective seroepidemiological analysis of patients with suspicion of paracoccidioidomycosis in São Paulo State, Brazil

Palavras-chave: Paracoccidioidomycosis; Heat shock protein; Serodiagnosis; ELISA; Western blotting.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400705

J. Venom. Anim. Toxins incl. Trop. Dis.

Kamikawa,CM; Kohara,VS; Passos,AN; Vicentini,AP.

The geographic distribution of paracoccidioidomycosis (PCM) in the Brazilian state of São Paulo was evaluated in a retrospective study using secondary data from serological analyses, carried out by double immunodiffusion assay of patients with PCM suspicion, from January 1999 to May 2010. Sixty percent of 10,176 patients, from 239 cities, were serologically reactive to P. brasiliensis. The cities that showed the most serological reactivity among patients were São João da Boa Vista (85%), Piracicaba (75%), Sorocaba (73%), Campinas (72%) and São Paulo (62%). São Paulo state has an area of 248,209.4 km²; the climate is tropical and sub-tropical with annual temperatures between 18 and 24ºC, high rainfall (900 to 1800 mm/year), rainy summers and mild winters....

Seroepidemiology of Paracoccidioides brasiliensis infection in horses from Rio Grande do Sul, Brazil

Palavras-chave: Paracoccidioides brasiliensis; Paracoccidioidomycosis; Immunological tests; Seroepidemiological studies.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300003

Albano,Ana Paula Neuschrank; Klafke,Gabriel Baracy; Brandolt,Tchana Martinez; Da Hora,Vanusa Pousada; Nogueira,Carlos Eduardo Wayne; Xavier,Melissa Orzechowski; Meireles,Mário Carlos Araújo.

Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and...

Standardization and validation of Dot-ELISA assay for Paracoccidioides brasiliensis antibody detection

Palavras-chave: ELISA; Gp-43; Protein G-peroxidase; Paracoccidioidomycosis.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200513

J. Venom. Anim. Toxins incl. Trop. Dis.

Kamikawa,Camila Mika; Mendes,Rinaldo Poncio; Vicentini,Adriana Pardini.

Abstract Background Paracoccidioidomycosis (PCM) is a neglected systemic mycosis caused by a dimorphic fungus of the Paracoccidioides genus. The standard diagnosis is based on isolation of the fungi in culture, and by microscopic visualization of characteristic multiple budding yeast cells in biological samples. However, in some situations, access to the site of injury prevents the collection of biological material. A variety of immuno-serological techniques has proven useful for allowing inferring diagnosis with a certain degree of certainty, thus optimizing time. The aim of this study was to standardize and validate the Dot-ELISA (DE) assay, comparing it with the serological standard, double immunodiffusion (DI). Methods In order to standardize the...

The left lung is preferentially targeted during experimental paracoccidioidomycosis in C57BL/6 mice

Palavras-chave: Paracoccidioidomycosis; Paracoccidioides brasiliensis; Immunodiagnostic tool; Dot-ELISA; Validation.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100305

BJMBR

Tristão,F.S.M.; Rocha,F.A.; Dias,F.C.; Rossi,M.A.; Silva,J.S..

Paracoccidioidomycosis (PCM) is a chronic systemic mycosis caused by the inhalation of the thermally dimorphic fungus Paracoccidioides brasiliensis as well as the recently described P. lutzii. Because the primary infection occurs in the lungs, we investigated the differential involvement of the right and left lungs in experimental P. brasiliensis infection. Lungs were collected from C57BL/6 mice at 70 days after intravenous infection with 1×106 yeast cells of a virulent strain of P. brasiliensis (Pb18). The left lung, which in mice is smaller and has fewer lobes than the right lung, yielded increased fungal recovery associated with a predominant interleukin-4 response and diminished synthesis of interferon-γ and nitric oxide compared with the right lung....

The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response

Palavras-chave: Lung; Paracoccidioidomycosis; Paracoccidioides brasiliensis.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000839

BJMBR

Almeida,S.R.; Lopes,J.D..

In the present study we evaluated T cell proliferation and Th lymphokine patterns in response to gp43 from Paracoccidioides brasiliensis presented by isolated dendritic cells from susceptible and resistant mice. T cell proliferation assays showed that dendritic cells from susceptible mice were less efficient than those from resistant mice. The pattern of T cell lymphokines stimulated by dendritic cells was always Th1, although the levels of IL-2 and IFN-gamma were lower in T cell cultures from susceptible mice. To determie whether different antigen-presenting cells such as macrophages and dendritic cells stimulated different concentrations of Th1 lymphokines, the production of IFN-gamma and IL-2 was measured. It was observed that dendritic cells were more...

USE OF HONEY AS NUTRITIONAL AND THERAPEUTIC SUPPLEMENT IN THE TREATMENT OF INFECTIOUS DISEASES

Palavras-chave: Dendritic cells; Paracoccidioidomycosis; Paracoccidioides brasiliensis; Th1/Th2.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400014

J. Venom. Anim. Toxins

PEREIRA,P.C.M.; BARRAVIERA,B.; BURINI,R.C.; SOARES,A.M.V.C.; BERTANI,M.A..

Honey, royal jelly, propolis, materials produced and/or gathered by Apis mellifera honeybees have been used as food and medication for centuries(6,7,8). Although extensively used, honeybee products have become a matter of interest and controversy for years. Honey, well known as healthy and natural food, has shown therapeutic properties in the treatment of digestive, respiratory, cardiac and rheumatic disorders, among others(4). Several studies have reported honey's immunological, antibacterial, antiinflammatory, antipyretic properties besides its importance in terms of energy intake(3,5). Furthermore, honey has proved to possesses wound healing and analgesic actions(1,2). Honey and propolis are also known to be effective in the treatment of respiratory...

Tipo: Info:eu-repo/semantics/report

Palavras-chave: Honey; Nutritional supplement; Apis mellifera; Paracoccidioidomycosis; Tuberculosis.

Ano: 1995

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000200006

Use of immunoblotting assay improves the sensitivity of paracoccidioidomycosis diagnosis

J. Venom. Anim. Toxins incl. Trop. Dis.

Silva,D. F.; Assis,C. M.; Zamboni,I. M.; Barreto,L. C.; Kohara,V. S.; Vicentini-Moreira,A. P..

The purpose of this work was to evaluate two serological assays: double immunodiffusion (DI) and immunoblotting (IB) in immunodiagnosis of paracoccidioidomycosis (PCM). We evaluated by IB assay 23 sera samples from patients with clinical confirmation of PCM, all of them with negative DI results against culture filtrate from Paracoccidioides brasiliensis isolate 113. For IB, as well as for comparative DI assay, we employed soluble components of the cell wall outer surface (SCCWOS) from P. brasiliensis isolate 113 cultivated at 36°C in Fava-Neto's agar medium for 5 and 10 days. Among the 20 sera samples analyzed by DI, 13 (65%) were negative and 7 (35%) were positive against SCCWOS obtained on the 5th and 10th days. By IB assay, 95.4% and 100% of sera...

Validation and clinical application of a nested PCR for paracoccidioidomycosis diagnosis in clinical samples from Colombian patients

Palavras-chave: Paracoccidioidomycosis; Immunodiffusion; Immunoblotting.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200009

Gaviria,Marcela; Rivera,Vanessa; Muñoz-Cadavid,Cesar; Cano,Luz Elena; Naranjo,Tonny Williams.

Paracoccidioidomycosis is a systemic and endemic mycosis, restricted to tropical and subtropical areas of Latin America. The infection is caused by the thermal dimorphic fungus Paracoccidioides brasiliensisand Paracoccidioides lutzii. The diagnosis of paracoccidioidomycosis is usually performed by microscopic examination, culture and immunodiagnostic tests to respiratory specimens, body fluids and/or biopsies; however these methods require laboratory personnel with experience and several days to produce a result. In the present study, we have validated and evaluated a nested PCR assay targeting the gene encoding the Paracoccidioides gp43membrane protein in 191 clinical samples: 115 samples from patients with proven infections other than...